LOCUS       NM_010137               5352 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus endothelial PAS domain protein 1 (Epas1), mRNA.
ACCESSION   NM_010137 XM_918126 XM_925520
VERSION     NM_010137.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Zou,X., Wang,K., Deng,Y., Guan,P., Pu,Q., Wang,Y., Mou,J., Du,Y.,
            Lou,X., Wang,S., Jiang,N., Zhou,S., Wang,H., Du,D., Liu,X., Hu,H.
            and Zhang,H.
  TITLE     Hypoxia-inducible factor 2alpha promotes pathogenic polarization of
            stem-like Th2 cells via modulation of phospholipid metabolism
  JOURNAL   Immunity 57 (12), 2808-2826 (2024)
   PUBMED   39609127
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Sekimoto,A., Takaso,Y., Saruyama,H., Ookawa,M., Yamamoto,M.,
            Toyohara,T., Saigusa,D., Fukuuchi,T., Otsuka,M., Fushiki,Y.,
            Yamakoshi,S., Tanaka,K., Ikeda,T., Tanaka,T., Takahashi,N.,
            Mishima,E. and Sato,E.
  TITLE     Impacts of low birthweight on kidney development and
            intergenerational growth of the offspring
  JOURNAL   iScience 27 (11), 111159 (2024)
   PUBMED   39524353
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5352)
  AUTHORS   Lee,S.Y., Kim,S.J., Park,K.H., Lee,G., Oh,Y., Ryu,J.H. and Huh,Y.H.
  TITLE     Differential but complementary roles of HIF-1alpha and HIF-2alpha
            in the regulation of bone homeostasis
  JOURNAL   Commun Biol 7 (1), 892 (2024)
   PUBMED   39039245
  REMARK    GeneRIF: Differential but complementary roles of HIF-1alpha and
            HIF-2alpha in the regulation of bone homeostasis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5352)
  AUTHORS   Schreiber,T., Koll,N., Padberg,C., de Los Reyes,B., Quinting,T.,
            Malyshkina,A., Metzen,E., Sutter,K., Fandrey,J. and Winning,S.
  TITLE     Reduced vacuolar ATPase protects mice from Friend virus infection -
            an unintended but instructive effect in Hif-2afl mice
  JOURNAL   J Cell Sci 137 (12) (2024)
   PUBMED   38856651
REFERENCE   5  (bases 1 to 5352)
  AUTHORS   Prajapati,M., Zhang,J.Z., Chiu,L., Chong,G.S., Mercadante,C.J.,
            Kowalski,H.L., Delaney,B., Anderson,J.A., Guo,S., Aghajan,M. and
            Bartnikas,T.B.
  TITLE     Hepatic HIF2 is a key determinant of manganese excess and
            polycythemia in SLC30A10 deficiency
  JOURNAL   JCI Insight 9 (10), e169738 (2024)
   PUBMED   38652538
  REMARK    GeneRIF: Hepatic HIF2 is a key determinant of manganese excess and
            polycythemia in SLC30A10 deficiency.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5352)
  AUTHORS   Jain,S., Maltepe,E., Lu,M.M., Simon,C. and Bradfield,C.A.
  TITLE     Expression of ARNT, ARNT2, HIF1 alpha, HIF2 alpha and Ah receptor
            mRNAs in the developing mouse
  JOURNAL   Mech Dev 73 (1), 117-123 (1998)
   PUBMED   9545558
REFERENCE   7  (bases 1 to 5352)
  AUTHORS   Ladoux,A. and Frelin,C.
  TITLE     Cardiac expressions of HIF-1 alpha and HLF/EPAS, two basic loop
            helix/PAS domain transcription factors involved in adaptative
            responses to hypoxic stresses
  JOURNAL   Biochem Biophys Res Commun 240 (3), 552-556 (1997)
   PUBMED   9398602
REFERENCE   8  (bases 1 to 5352)
  AUTHORS   Ema,M., Taya,S., Yokotani,N., Sogawa,K., Matsuda,Y. and
            Fujii-Kuriyama,Y.
  TITLE     A novel bHLH-PAS factor with close sequence similarity to
            hypoxia-inducible factor 1alpha regulates the VEGF expression and
            is potentially involved in lung and vascular development
  JOURNAL   Proc Natl Acad Sci U S A 94 (9), 4273-4278 (1997)
   PUBMED   9113979
REFERENCE   9  (bases 1 to 5352)
  AUTHORS   Flamme,I., Frohlich,T., von Reutern,M., Kappel,A., Damert,A. and
            Risau,W.
  TITLE     HRF, a putative basic helix-loop-helix-PAS-domain transcription
            factor is closely related to hypoxia-inducible factor-1 alpha and
            developmentally expressed in blood vessels
  JOURNAL   Mech Dev 63 (1), 51-60 (1997)
   PUBMED   9178256
REFERENCE   10 (bases 1 to 5352)
  AUTHORS   Tian,H., McKnight,S.L. and Russell,D.W.
  TITLE     Endothelial PAS domain protein 1 (EPAS1), a transcription factor
            selectively expressed in endothelial cells
  JOURNAL   Genes Dev 11 (1), 72-82 (1997)
   PUBMED   9000051
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK136911.1, AK141185.1, U81983.1, D89787.1 and AC096777.1.
            
            On Apr 8, 2008 this sequence version replaced NM_010137.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK136911.1, SRR1660811.18563.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-264               AK136911.1         1-264
            265-1365            AK141185.1         2-1102
            1366-2424           U81983.1           1049-2107
            2425-3210           D89787.1           2387-3172
            3211-5352           AC096777.1         202978-205119
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 56.9 cM"
     gene            1..5352
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /note="endothelial PAS domain protein 1"
                     /db_xref="GeneID:13819"
                     /db_xref="MGI:MGI:109169"
     exon            1..445
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             420..3044
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /note="mHLF; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-related
                     factor; HIF-1-alpha-like factor; hypoxia-inducible factor
                     2-alpha; HLF (HIF1alpha-like factor); hypoxia inducible
                     transcription factor 2alpha; HIF1 alpha-like factor; Hif
                     like protein"
                     /codon_start=1
                     /product="endothelial PAS domain-containing protein 1"
                     /protein_id="NP_034267.3"
                     /db_xref="CCDS:CCDS37713.1"
                     /db_xref="GeneID:13819"
                     /db_xref="MGI:MGI:109169"
                     /translation="MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLP
                     HSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVV
                     TQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLTLKNGSGFGKKSK
                     DVSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSSLCGSKEPL
                     LSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELIGYHPEELLGRS
                     AYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQP
                     QCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSDDVAVTEKSNYLFTK
                     LKEEPEELAQLAPTPGDAIISLDFGSQNFDEPSAYGKAILPPGQPWVSGLRSHSAQSE
                     SGSLPAFTVPQADTPGNTTPSASSSSSCSTPSSPEDYYSSLENPLKIEVIEKLFAMDT
                     EPRDPGSTQTDFSELDLETLAPYIPMDGEDFQLSPICPEEPLMPESPQPTPQHCFSTM
                     TSIFQPLTPGATHGPFFLDKYPQQLESRKTESEHWPMSSIFFDAGSKGSLSPCCGQAS
                     TPLSSMGGRSNTQWPPDPPLHFGPTKWPVGDQSAESLGALPVGSSQLEPPSAPPHVSM
                     FKMRSAKDFGARGPYMMSPAMIALSNKLKLKRQLEYEEQAFQDTSGGDPPGTSSSHLM
                     WKRMKSLMGGTCPLMPDKTISANMAPDEFTQKSMRGLGQPLRHLPPPQPPSTRSSGEN
                     AKTGFPPQCYASQFQDYGPPGAQKVSGVASRLLGPSFEPYLLPELTRYDCEVNVPVPG
                     SSTLLQGRDLLRALDQAT"
     exon            446..636
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            637..788
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            789..873
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            874..992
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1198
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1305
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1453
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1668
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1859
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1970
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2476
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2603
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2718
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2719..2892
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..5352
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5333..5338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /note="hexamer: ATTAAA"
     polyA_site      5352
                     /gene="Epas1"
                     /gene_synonym="bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2"
                     /note="major polyA site"
ORIGIN      
        1 ttttttttct ttttttcttt ttcttttttt ctttttcttt tttggttggt tggttttgat
       61 ttgtcagatc ccagaaaagt gactcctgtt cggggctaaa cggaactcca ggtcccttgt
      121 cgctgctctc tctctctttt ggcgtcttac aacctcctcc cactcctttc cccggccccg
      181 cctcctcctg caggttcctc cccgtcaccc ccctcctccc tcctcctcct ccgcacctag
      241 ccagccctct gcaaacttcc acctgattga gcgggactct cggacctgcg agcactaaag
      301 acctttcaca cctgcccggg cgacagagag ctgcggaggg ccacagcaaa gagagcggct
      361 gcagccccta cggggttaag gaacccaggt gctccgggtc tcggagggcc acggcgacaa
      421 tgacagctga caaggagaaa aaaaggagca gctcagagct gaggaaggag aaatcccgtg
      481 atgccgcgag gtgccggcgc agcaaggaga cggaggtctt ctatgagttg gctcatgagt
      541 tgcccctgcc tcacagtgtg agctcccacc tggacaaagc ctccatcatg cgcctggcca
      601 tcagcttcct tcggacacat aagctcctgt cctcagtctg ctctgaaaat gaatctgaag
      661 ctgaggccga ccagcaaatg gataacttgt acctgaaagc cttggagggt ttcattgctg
      721 tggtgaccca agacggtgac atgatctttc tgtcggaaaa catcagcaag ttcatgggac
      781 ttactcaggt agaactaaca ggacacagca tctttgactt cactcatcct tgcgaccatg
      841 aggagatccg tgagaacctg actctcaaaa acggctctgg ttttgggaag aagagcaaag
      901 acgtgtccac cgagcgtgac ttcttcatga ggatgaagtg cacggtcacc aacagaggcc
      961 ggactgtcaa cctcaagtcg gccacctgga aggtcctgca ctgcaccggg caagtgagag
     1021 tctacaacaa ctgcccccct cacagtagcc tctgtggctc caaggagccc ctgctgtcct
     1081 gccttatcat catgtgtgag ccaatccagc acccatccca catggacatc cccctggaca
     1141 gcaagacttt cctgagccgc cacagcatgg acatgaagtt cacctactgt gacgacagaa
     1201 tcttggaact gattggttac caccccgagg agctacttgg acgctctgcc tatgagttct
     1261 accatgccct ggattcggag aacatgacca aaagtcacca gaacttgtgc accaaggggc
     1321 aggtggtatc tggccagtac cggatgctag ccaaacacgg aggatatgtg tggctggaga
     1381 cccaggggac ggtcatctac aacccccgca acctgcagcc tcagtgtatc atgtgtgtca
     1441 actatgtgct gagtgagatc gagaagaacg acgtggtgtt ctccatggac cagaccgaat
     1501 ccctgttcaa gccacacctg atggccatga acagcatctt tgacagcagt gacgatgtgg
     1561 ctgtaactga gaagagcaac tacctgttca ccaaactgaa ggaggagccc gaggaactgg
     1621 cccagttggc ccccacccca ggagatgcca ttatttctct cgatttcgga agccagaact
     1681 tcgatgaacc ctcagcctat ggcaaggcca tccttccccc gggccagcca tgggtctcgg
     1741 ggctgaggag ccacagtgcc cagagcgagt ccgggagcct gccagccttc actgtgcccc
     1801 aggcagacac cccagggaac actacaccca gtgcttcaag cagcagtagc tgctccacgc
     1861 ccagcagccc tgaggactac tattcatcct tggagaatcc cttgaagatc gaagtgattg
     1921 agaagctttt cgccatggac acggagccga gggacccggg cagtacccag acggacttca
     1981 gtgaactgga tttggagacc ttggcaccct acatccctat ggacggcgag gacttccagc
     2041 tgagccccat ctgcccagag gagccgctca tgccagagag cccccagccc accccccagc
     2101 actgcttcag taccatgacc agcatcttcc agccgctcac cccgggggcc acccacggcc
     2161 ccttcttcct cgataagtac ccgcagcagt tggaaagcag gaagacagag tctgagcact
     2221 ggcccatgtc ttccatcttc tttgatgctg ggagcaaagg gtccctgtct ccatgctgtg
     2281 gccaggccag cacccctctc tcttctatgg gaggcagatc caacacgcag tggcccccgg
     2341 atccaccatt acatttcggc cctactaagt ggcctgtggg tgatcagagt gctgaatccc
     2401 tgggagccct gccggtgggg tcatcgcagt tggaacctcc gagcgccccg cctcatgtct
     2461 ccatgttcaa gatgaggtct gcaaaggact tcggggcccg aggtccatac atgatgagcc
     2521 cagccatgat cgccctgtcc aacaagctga agctaaagcg gcagctggag tatgaggagc
     2581 aagccttcca agacacaagc gggggggacc ctccaggcac cagcagttca cacttgatgt
     2641 ggaaacgtat gaagagcctc atgggcggga cctgtccttt gatgcctgac aagaccatca
     2701 gtgcgaacat ggcccccgat gaattcaccc aaaaatctat gagaggcctg ggccagccac
     2761 tgagacacct gccacctccc cagccaccat ctaccaggag ctcaggggag aacgccaaga
     2821 ctgggttccc gccacagtgc tatgcctccc agttccagga ctacggtcct ccaggagctc
     2881 aaaaggtgtc aggcgtggcc agtcgactgc tggggccatc gttcgagcct tacctgttgc
     2941 cggaactgac cagatatgac tgtgaggtga acgtgcccgt gcctggaagc tccacactcc
     3001 tgcaggggag agaccttctc agagctctgg accaggccac ctgagccagg gcctctggcc
     3061 gggcatgccc ctgcctgccc cgccgtcttg acctgccagc ttcacttcca tctgtgttgc
     3121 tattaggtat ctctaacacc agcacacttc ttacgagatg tactcaacct ggcctactgg
     3181 ccaggtcacc aagcagtggc ctttatctga catgctcact ttattatcca tgttttaaaa
     3241 atacatagtt gttgtacctg ctatgtttta ccgttgatga aagtgttctg aaattttata
     3301 agatttcccc ctccctccct cccttgaatt acttctaatt tatattcccc aaaggttttt
     3361 ctctctctca ttcatatcca tactaacaag catggtggct ggtgcctctc cctaggaaag
     3421 ctttggcgtc attcaactca agtgttcttg ttcttgttgc caaagagaaa aggattttcc
     3481 tccactgtgg attctccctc tcccccaccc ccacatacac acacacacac acacacccct
     3541 acacacatat acacacatgc acgtatgcgt gcacacacac acacacacac acacacacac
     3601 acacacaccc ctacacacac acacacacac acacatatac acacacacac acacacacac
     3661 acacacccct acacacatat acacacatgc acgtatgcgt gcacacacac acacacacac
     3721 acacacaccc ctacacacat atacacacat gcacgtatgc gtgcacacac acacacacac
     3781 acacacatct aatcaccata ttgtaaaatt ttgtgttttt aaagccaact ctttgctccg
     3841 gttttttcat acgacttagt atggggcaaa aaagcaatgt gaagaatcaa ctctagggtt
     3901 acctgtgaag ccacgcggtg gtgttcgaag ctgtctggta atgcccccat ctctccccgg
     3961 gtccagtgga tttttttaac tattattcaa aagcaaaact gagttttgtt ttgtttggtt
     4021 ttttaagaag aatttatatc cgggtttagt gtttatcata tatatgggta ctctgtaata
     4081 tctaaaagct tagaaatggg atcttgctca caagaatcac ttttaagatc ttttagggct
     4141 gttaatttta tttttctcag tgttctggac actgtagacc tgtgcagtac tcccacaggc
     4201 ctgactaacc gccacagatc tctctttctt tctctctggt gactttttgc ttttgtgata
     4261 tggcagcggt gtgacagtcc caggagagaa gtcattaagg gggaacattg caagctatct
     4321 tttcgtgttc tcagtgtatt atgtacgtgt gtgctgttgc cgctatgagg gctcggggct
     4381 tgcaggctct gagggcatgt cgcaggcgag ctggaaggaa gcgctctcac tttttttcag
     4441 gcaatgttgc tagcccttct agcacactga gctacgagac tgccaatctc tctcctgcgg
     4501 cacgtttggg ttggcacagc taccatgaaa tgtttttaat gggaattcat aaagcaagtt
     4561 aggattcaca aatgtagtga cctagtcctt ggagctaaac tgggagtggg tgccaggtgg
     4621 agggtagcag gtctgaccag ccctacaccc tgccacagat cacagcttgc cgggcaaatg
     4681 accctgcgaa gccacggtgc agcgccccct gctggcgagc agcgggcaga cctccaagga
     4741 cagcctctag ctgaggtctc agtcacatga agtgtgacac tgaacaggta gaaagcactg
     4801 aaaatcgtgt tccaagagca ctttgtaact cactgagggg gaggggaagc accttttggg
     4861 tttgcgatac caatcaacat aaggtgttgt tgactcgggt acaataaaac tctcaacaca
     4921 cacaaagccc atcaggcaag agtacagtaa gcctaagggg acttgctgct caaaccaaaa
     4981 attaactctg aaggtcagtc tacctgacat tacctaggca tggtgttgtc caagctgcgc
     5041 atcggtgcac agtgtggcct ggcacacacg caggcgggcg acggaacttg aagggttatt
     5101 gacatgcaaa tgctggtgtt tgatttcctg tgttgttgcc tcagcgttaa gggcatttcc
     5161 gtttgcagtt ctactaaaga cactctgaga aatattccga gtttcgtatt aacctttcct
     5221 gtccatgtaa caacttcatg accattactg cattgtcaaa ttcctactga cgacattata
     5281 actgtacggg agcttaactt tataaggaaa tgtattttga cactgatatc ttattaaagt
     5341 attctgatcc ta
//
