LOCUS       NM_028093               2306 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ectonucleoside triphosphate diphosphohydrolase 8
            (Entpd8), mRNA.
ACCESSION   NM_028093 XM_619737 XM_913447 XM_975922 XM_975956 XM_975996
            XM_976205 XM_986744
VERSION     NM_028093.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Salem,M., Lecka,J., Pelletier,J., Gomes Marconato,D., Dumas,A.,
            Vallieres,L., Brochu,G., Robaye,B., Jobin,C. and Sevigny,J.
  TITLE     NTPDase8 protects mice from intestinal inflammation by limiting
            P2Y6 receptor activation: identification of a new pathway of
            inflammation for the potential treatment of IBD
  JOURNAL   Gut 71 (1), 43-54 (2022)
   PUBMED   33452178
  REMARK    GeneRIF: NTPDase8 protects mice from intestinal inflammation by
            limiting P2Y6 receptor activation: identification of a new pathway
            of inflammation for the potential treatment of IBD.
REFERENCE   3  (bases 1 to 2306)
  AUTHORS   Tani,H., Li,B., Kusu,T., Okumura,R., Nishimura,J., Okuzaki,D.,
            Motooka,D., Arakawa,S., Mori,A., Yoshihara,T., Ogino,T., Tsai,S.H.,
            Furuta,Y., Muneta,M., Nakamura,S., Fukusaki,E., Yamamoto,K.,
            Yagita,H., Kayama,H. and Takeda,K.
  TITLE     The ATP-hydrolyzing ectoenzyme E-NTPD8 attenuates colitis through
            modulation of P2X4 receptor-dependent metabolism in myeloid cells
  JOURNAL   Proc Natl Acad Sci U S A 118 (39) (2021)
   PUBMED   34548395
  REMARK    GeneRIF: The ATP-hydrolyzing ectoenzyme E-NTPD8 attenuates colitis
            through modulation of P2X4 receptor-dependent metabolism in myeloid
            cells.
REFERENCE   4  (bases 1 to 2306)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 2306)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 2306)
  AUTHORS   Munkonda,M.N., Kauffenstein,G., Kukulski,F., Levesque,S.A.,
            Legendre,C., Pelletier,J., Lavoie,E.G., Lecka,J. and Sevigny,J.
  TITLE     Inhibition of human and mouse plasma membrane bound NTPDases by P2
            receptor antagonists
  JOURNAL   Biochem Pharmacol 74 (10), 1524-1534 (2007)
   PUBMED   17727821
REFERENCE   7  (bases 1 to 2306)
  AUTHORS   Fausther,M., Lecka,J., Kukulski,F., Levesque,S.A., Pelletier,J.,
            Zimmermann,H., Dranoff,J.A. and Sevigny,J.
  TITLE     Cloning, purification, and identification of the liver canalicular
            ecto-ATPase as NTPDase8
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 292 (3), G785-G795 (2007)
   PUBMED   17095758
REFERENCE   8  (bases 1 to 2306)
  AUTHORS   Kukulski,F., Levesque,S.A., Lavoie,E.G., Lecka,J., Bigonnesse,F.,
            Knowles,A.F., Robson,S.C., Kirley,T.L. and Sevigny,J.
  TITLE     Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3
            and 8
  JOURNAL   Purinergic Signal 1 (2), 193-204 (2005)
   PUBMED   18404504
  REMARK    Erratum:[Purinergic Signal. 2005 Jul 29;1(3):293]
REFERENCE   9  (bases 1 to 2306)
  AUTHORS   Bigonnesse,F., Levesque,S.A., Kukulski,F., Lecka,J., Robson,S.C.,
            Fernandes,M.J. and Sevigny,J.
  TITLE     Cloning and characterization of mouse nucleoside triphosphate
            diphosphohydrolase-8
  JOURNAL   Biochemistry 43 (18), 5511-5519 (2004)
   PUBMED   15122917
  REMARK    GeneRIF: Enzymatic properties of NTPDase8 (Entpd8) differ from
            those of other NTPDases and suggest an alternative way to modulate
            nucleotide levels and consequently P2Y and P2X receptor activation.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB610017.1, AY364442.1 and AV026718.2.
            
            On or before Aug 25, 2006 this sequence version replaced
            XM_913447.2, XM_986744.1, XM_619737.3, XM_976205.1, XM_975996.1,
            XM_975922.1, XM_975956.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY364442.1, SRR5189682.80515.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-138               BB610017.1         21-158
            139-1924            AY364442.1         1-1786
            1925-2306           AV026718.2         226-607
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 16.97 cM"
     gene            1..2306
                     /gene="Entpd8"
                     /note="ectonucleoside triphosphate diphosphohydrolase 8"
                     /db_xref="GeneID:72090"
                     /db_xref="MGI:MGI:1919340"
     exon            1..229
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            230..371
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..1739
                     /gene="Entpd8"
                     /EC_number="3.6.1.5"
                     /note="NTPDase8; NTPDase 8; E-NTPDase 8"
                     /codon_start=1
                     /product="ectonucleoside triphosphate diphosphohydrolase
                     8"
                     /protein_id="NP_082369.1"
                     /db_xref="CCDS:CCDS15748.1"
                     /db_xref="GeneID:72090"
                     /db_xref="MGI:MGI:1919340"
                     /translation="MGLSWKERVFMALLGVAAASGLTMLVLILVKAINVLLPADTKFG
                     IVFDAGSSHTSLFVYQWPANKEKDTGVVSQALTCQIEGPGISSYTSDPTQAGESLKSC
                     LEEALALIPQAQHPETPTFLGSTAGMRLLSQKNSSQARDILAAVSQTLSKSPVDFWGA
                     KILAGQDEGAFGWITINYVLGMLLKYSSGQWILPEEGMLVGALDLGGASTQISFVPQG
                     PILDQSTQVTFRLYGANYSVYTHSYLCFGRDQILNRLLAKLAQDRLSSQVAPVRHPCY
                     HSGYQAILPLSSLYDSPCIHTTDSLNHTQNLTVEGTGDPGNCVVALRSLFNFSSCKGQ
                     KDCAFNGIYQPPVHGQFYAFSNFYYTFHFLNLTSRQSLNTVNDTVWKFCQKPWKLVEV
                     SYPGQERWLRDYCASGLYILVLLLEGYKFSEETWPNIQFQKQAGDTDIGWTLGFMLNL
                     TGMIPAEAPTHWRAQSYSIWTAGVVFAVLTLVAILGAAAIQIFWTQD"
     exon            372..489
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            490..640
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            641..800
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            801..1037
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1301
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1412
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1547
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..2306
                     /gene="Entpd8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2280..2285
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Entpd8"
                     /note="hexamer: ATTAAA"
     polyA_site      2303
                     /gene="Entpd8"
                     /note="major polyA site"
ORIGIN      
        1 acaactaggg acgctgctct tcctccctcc acaggagcaa gatgtaagcc tggaattaat
       61 gagatagagt aaaattagta gatgccagag cagcagctag agagcagcct gctcctccca
      121 ccacagcccc aggttcaagt aggtggagac ggggtgtgga ggacagcagg tgtgcaggtc
      181 agaagcagaa cttcctcaag ctatccttga aagttggctc ccagtgcagc tcagaccctc
      241 tcaccatggg actctcctgg aaggaacggg tcttcatggc tctgttggga gttgcagcag
      301 cctctggcct caccatgctc gtcctcatcc tggtgaaggc aatcaatgtt ctcttgcctg
      361 cagacaccaa gtttgggatt gtgtttgatg ccggctcctc ccacacatcc ctgtttgtgt
      421 accagtggcc agcaaacaag gagaaggaca caggagtggt cagccaggcc ctgacttgcc
      481 agatagaagg acctggaatc tcttcctata cctctgaccc gacacaggct ggggaaagcc
      541 tgaagagctg cctggaggag gcgctggcgt tgatcccaca ggcccagcat ccagagacgc
      601 ccacattctt ggggtccaca gcaggaatga ggctgctcag ccagaagaac agctctcagg
      661 caagagacat cctagctgca gtctcccaga cactaagcaa gtctcctgtg gatttttggg
      721 gtgctaagat cttggctggg caggatgaag gtgcctttgg ttggatcacc atcaactatg
      781 tcctgggaat gctcctgaag tattcctctg gacagtggat cctgcctgaa gaggggatgc
      841 tagttggtgc tctggacctt ggtggagcct ccacgcagat cagcttcgtg cctcagggcc
      901 ccatcctgga ccagagcacc caagtcacct tccgcctgta cggtgccaac tacagtgtct
      961 acactcacag ctacctctgc tttgggcggg accagatcct gaacaggctc ctggctaagc
     1021 tggcacagga caggttgagc agccaggtgg ccccggtcag acacccatgc taccacagtg
     1081 gctaccaggc catactgcca ctgagttcct tgtatgactc accctgcatc cacactacag
     1141 attccctgaa ccacacccag aacctcacag ttgaagggac aggcgaccct gggaactgtg
     1201 tggtagctct ccgaagtctc ttcaacttct ccagctgtaa gggccagaag gattgtgctt
     1261 tcaatggcat ctaccagcct cctgtgcacg gccagttcta tgcattttcc aacttttact
     1321 acaccttcca tttcctgaac ctcacgtcca ggcaatcact gaacactgtc aacgacactg
     1381 tctggaagtt ctgtcagaaa ccctggaaac tggtggaagt cagctatcct gggcaggagc
     1441 gctggttacg ggactactgt gcctcgggtc tgtacatcct cgtattgctg ctggagggct
     1501 acaaattcag tgaggagacc tggcccaaca tccagttcca gaagcaggca ggtgacacag
     1561 acattggctg gacactgggc ttcatgctga acctgacagg catgattcca gctgaggcac
     1621 cgacccactg gcgggctcag agctacagca tctggacggc tggagtagta ttcgcagtgc
     1681 tgacccttgt ggccattctt ggggcagctg ccatccagat cttctggacc caggactagg
     1741 tagaaaccaa gctgaagtag atcacagtca acaatatcta aagcagaagt gttgagtcaa
     1801 cagtgacaac acagctgttt gtgttgtcaa ccttgtgatc caccatcaac tgttcttagg
     1861 aaagaaggcc tttggcacac aggaccttct gagcagatcc tagatgcctg cccttatgaa
     1921 cccctgacct ttaaggactc cagtccatca attgggccag gagggccaat tgctgatagt
     1981 actgactcct ttatgccctc aggatagcac tggctatggg gactgatgtt gtccatagga
     2041 tagaaacagg agaggcagct gttggaatag tgccacctca gaaggctgtg gccagcccag
     2101 cagagactcc ctgcttcctc actatatcag actagaccag acagtcagag tccagctctg
     2161 gggccagccc agcttcttca cacaagagcc tgtggttgga ttctctttat catcaatccc
     2221 agaatataga tcaagttggg aatgagggaa tcatttttgt ttgcttgttt gcttgtttaa
     2281 ttaaagctgg ttttgtcatg gtattt
//
