LOCUS       NM_177304               4984 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 6
            (Enpp6), mRNA.
ACCESSION   NM_177304
VERSION     NM_177304.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Jing,J., Feng,J., Yuan,Y., Guo,T., Lei,J., Pei,F., Ho,T.V. and
            Chai,Y.
  TITLE     Spatiotemporal single-cell regulatory atlas reveals neural crest
            lineage diversification and cellular function during tooth
            morphogenesis
  JOURNAL   Nat Commun 13 (1), 4803 (2022)
   PUBMED   35974052
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Talley,M.J., Nardini,D., Shabbir,N., Ehrman,L.A., Prada,C.E. and
            Waclaw,R.R.
  TITLE     Generation of a Mouse Model to Study the Noonan Syndrome Gene Lztr1
            in the Telencephalon
  JOURNAL   Front Cell Dev Biol 9, 673995 (2021)
   PUBMED   34222248
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4984)
  AUTHORS   Morita,J., Kano,K., Kato,K., Takita,H., Sakagami,H., Yamamoto,Y.,
            Mihara,E., Ueda,H., Sato,T., Tokuyama,H., Arai,H., Asou,H.,
            Takagi,J., Ishitani,R., Nishimasu,H., Nureki,O. and Aoki,J.
  TITLE     Structure and biological function of ENPP6, a choline-specific
            glycerophosphodiester-phosphodiesterase
  JOURNAL   Sci Rep 6, 20995 (2016)
   PUBMED   26888014
  REMARK    GeneRIF: The crystal structure of ENPP6 in complex with
            phosphocholine revealed that the choline moiety of the
            phosphocholine is recognized by a choline-binding pocket formed by
            conserved aromatic and acidic residues. The present study provides
            the molecular basis for ENPP6-mediated choline metabolism at
            atomic, cellular and tissue levels.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4984)
  AUTHORS   Morita,J., Kato,K., Mihara,E., Ishitani,R., Takagi,J.,
            Nishimasu,H., Aoki,J. and Nureki,O.
  TITLE     Expression, purification, crystallization and preliminary X-ray
            crystallographic analysis of Enpp6
  JOURNAL   Acta Crystallogr F Struct Biol Commun 70 (Pt 6), 794-799 (2014)
   PUBMED   24915096
REFERENCE   5  (bases 1 to 4984)
  AUTHORS   Song,Y.B., An,Y.R., Kim,S.J., Park,H.W., Jung,J.W., Kyung,J.S.,
            Hwang,S.Y. and Kim,Y.S.
  TITLE     Lipid metabolic effect of Korean red ginseng extract in mice fed on
            a high-fat diet
  JOURNAL   J Sci Food Agric 92 (2), 388-396 (2012)
   PUBMED   21918993
REFERENCE   6  (bases 1 to 4984)
  AUTHORS   Kawaguchi,M., Okabe,T., Okudaira,S., Hanaoka,K., Fujikawa,Y.,
            Terai,T., Komatsu,T., Kojima,H., Aoki,J. and Nagano,T.
  TITLE     Fluorescence probe for lysophospholipase C/NPP6 activity and a
            potent NPP6 inhibitor
  JOURNAL   J Am Chem Soc 133 (31), 12021-12030 (2011)
   PUBMED   21721554
REFERENCE   7  (bases 1 to 4984)
  AUTHORS   Sakagami,H., Aoki,J., Natori,Y., Nishikawa,K., Kakehi,Y., Natori,Y.
            and Arai,H.
  TITLE     Biochemical and molecular characterization of a novel
            choline-specific glycerophosphodiester phosphodiesterase belonging
            to the nucleotide pyrophosphatase/phosphodiesterase family
  JOURNAL   J Biol Chem 280 (24), 23084-23093 (2005)
   PUBMED   15788404
  REMARK    GeneRIF: NPP6 has a specific role through the hydrolysis of
            polyunsaturated LPC, glycerophosphorylcholine, or
            sphingosylphosphorylcholine in kidney, brain and heart
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC123751.6, AK046797.1,
            AC160460.5 and BE991175.1.
            
            On Jul 21, 2016 this sequence version replaced NM_177304.3.
            
            Summary: This gene encodes a member of the nucleotide
            pyrophosphatase/phosphodiesterase family of enzymes that play an
            important role in regulating extracellular nucleotide metabolism.
            The encoded preproprotein undergoes proteolytic processing to
            generate a glycosylphosphatidylinositol (GPI)-anchored membrane
            protein that hydrolyzes choline-containing lysophospholipids such
            as glycerophosphocholine. Mice lacking the encoded protein develop
            fatty liver and myelin sheath abnormalities. [provided by RefSeq,
            Jul 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046797.1, AK046881.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AC123751.6         158768-158805
            39-3103             AK046797.1         2-3066
            3104-4943           AC160460.5         142801-144640
            4944-4984           BE991175.1         1-41                c
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 26.48 cM"
     gene            1..4984
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /note="ectonucleotide pyrophosphatase/phosphodiesterase 6"
                     /db_xref="GeneID:320981"
                     /db_xref="MGI:MGI:2445171"
     exon            1..325
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1407
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /EC_number="3.1.4.38"
                     /note="ectonucleotide pyrophosphatase/phosphodiesterase
                     family member 6; choline-specific glycerophosphodiester
                     phosphodiesterase; glycerophosphocholine
                     cholinephosphodiesterase ENPP6"
                     /codon_start=1
                     /product="glycerophosphocholine cholinephosphodiesterase
                     ENPP6 preproprotein"
                     /protein_id="NP_796278.1"
                     /db_xref="CCDS:CCDS40335.1"
                     /db_xref="GeneID:320981"
                     /db_xref="MGI:MGI:2445171"
                     /translation="MAAKLWTFLLGFGLSWVWPASAHRKLLVLLLDGFRSDYISEDAL
                     ASLPGFREIVNRGVKVDYLTPDFPSLSYPNYYTLMTGRHCEVHQMIGNYMWDPRTNKS
                     FDIGVNRDSLMPLWWNGSEPLWITLMKARRKVYMYYWPGCEVEILGVRPTYCLEYKTV
                     PTDINFANAVSDALDSLKSGRADLAAIYHERIDVEGHHYGPSSPQRKDALRAVDTVLK
                     YMIQWIQDRGLQQDLNVILFSDHGMTDIFWMDKVIELSNYISLDDLQQVKDRGPVVSL
                     WPVPGKHSEIYHKLRTVEHMTVYEKESIPNRFYYKKGKFVSPLTLVADEGWFIAESRE
                     MLPFWMNSTGKREGWQRGWHGYDNELMDMRGIFLAIGPDFKSNFRAAPIRSVDVYNIM
                     CHVAGITPLPNNGSWSRVVCMLKGQTSSAPPTPLNSCALVLILLLYFV"
     exon            326..505
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     exon            506..617
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     exon            618..759
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     exon            760..939
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1077
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1201
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..4970
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1441..1446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /note="hexamer: AGTAAA"
     polyA_site      1463
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /note="major polyA site"
     regulatory      1487..1492
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
                     /note="hexamer: AATAAT"
     polyA_site      1508
                     /gene="Enpp6"
                     /gene_synonym="4833421B01Rik; B830047L21Rik; D8Ertd514e;
                     E-NPP 6; GPC-Cpde; Npp6"
ORIGIN      
        1 agatgagaga ataactccag actccatcct caggctttgc aagggcacag ctgtgccagc
       61 tcaccctggc agactcctgg cagcatggca gcaaagctct ggaccttcct gctgggcttt
      121 gggctcagct gggtgtggcc ggcttctgcc caccggaagc tcctggtgtt gctcctggat
      181 ggttttcgct cagactacat cagtgaggat gctctagcat ccttgcctgg cttcagagag
      241 attgtgaaca gaggcgtcaa agtggattac ttgactccag actttcccag cctctcctat
      301 cccaattact acaccctcat gactggccgc cactgtgagg tccaccagat gatcggcaac
      361 tacatgtggg atcccagaac caacaagtca tttgacatcg gggtcaaccg agacagcctg
      421 atgcccctgt ggtggaacgg gtcagaaccg ctgtggatca ctctgatgaa agccaggagg
      481 aaagtctaca tgtattactg gcccggctgt gaagttgaga ttcttggtgt cagaccaact
      541 tactgcctag aatataaaac tgtcccaaca gatatcaact ttgcgaatgc agttagcgat
      601 gctctcgact cattaaagag tggccgagcg gatctagcag ccatatacca tgagcgcatc
      661 gatgtagaag gtcaccacta cggcccttca tcacctcaga gaaaagatgc cctcagagct
      721 gtggacactg tcctgaagta tatgatccag tggattcagg accgaggcct gcagcaggac
      781 ctaaacgtca tcctcttctc agaccatggg atgactgaca tcttctggat ggataaagtg
      841 attgagctga gcaactacat cagcctggac gacctgcagc aagtgaaaga ccgagggccc
      901 gttgtgagcc tgtggccagt tcctggaaaa cactctgaga tatatcacaa actccgcaca
      961 gtggaacaca tgaccgtgta tgagaaagaa tcaataccca acaggttcta ttacaagaaa
     1021 ggaaaatttg tctctccttt gaccctggtg gctgatgaag gatggttcat agcagagagt
     1081 cgagagatgc ttccattttg gatgaacagc acgggcaagc gtgaaggctg gcagcgagga
     1141 tggcacggat atgacaacga actcatggac atgagaggca tcttcctggc catcggacct
     1201 gatttcaagt ccaacttcag agctgctcca atcagatccg tggatgtcta caacatcatg
     1261 tgccacgtcg caggcatcac cccactgccc aacaacgggt cctggtccag ggtggtatgc
     1321 atgctgaagg gccagaccag ctctgctcca cccaccccac tgaacagctg tgcactggtc
     1381 ttgattctcc tcttatactt tgtatagctg gccctatggc tcattccaaa gcactgttgc
     1441 agtaaagcct gcttccaaca tgggacagtt ttcattttct ttatggaata atagctttat
     1501 taacacaatc aaggccgtta aagttgtgaa tatattattc ttgggtgatt ctacccacaa
     1561 aagtcccttc tggggaaaaa aactgcaaaa ttcgtatact ttgttttacc taaaaagttt
     1621 gaaatttgca tcttctcatt cacttttcta catagttctc tgctttgttt atacatcctt
     1681 actgaagatg aacagtgaga gccatgcttt gcccctgcaa caggcaaaca ttaaacgggt
     1741 atcctgtagt catcttggac cctctcatac tgccttgccc tgtcaagcag gctctctgtg
     1801 atctgtccac atgtaagagc tcacacttgg aggtctgcag ccataatgtt atattttcta
     1861 ttctattcca gatagagaca gtctatctac aggaaagcaa tatgtgttgt ctggttcttc
     1921 cccaatgcat ggtggttttt ttttgttttt tgttttttgt ttttttaaca acacaatctc
     1981 agaaagcaca tagaccagta gaaatcacaa ccaattgaag tatcaataac aattagacaa
     2041 ctcatagaac tggcagaggc catgacacct ctcaaaattt gagaacagaa tagcttctcc
     2101 ccttcattga atgccaagtt tcaaggttct atgtaactaa aaacacaatc tctcataata
     2161 caaagccttg tgagccacag gccctgaaca ttgacatcca catctgcctg tcggtgacca
     2221 ctaacttccc tcagacactg ctactctttt agtttttgag tcaaggcaca tgtctgatat
     2281 gtcagtgaag gcttggaaag gaagccttag tatcatagta tgaacatcat aaatttaact
     2341 ctttgctagg aatagtgata tatcaaatgc aagttccata cttcagtagt tcagaaggac
     2401 cttgtaaaac aaggactgat tgtactttta aacaattaga agagagccta catgtacaca
     2461 cacatgcaca cacatttaca cataaacaca tatacacatg cacacacaca tgtatgcaca
     2521 tgcacagaca taaacatata catgaatata aacacacaca cacgtgtgtg aacacatgcc
     2581 ctccacatat tatggtgata cctagaattt ttatcttttt tatgatgaag aagcatcata
     2641 aagcatcaaa ttcaaaagaa tttgagtttt gaatttgtcc attttctcag ctactgttat
     2701 gccatgtgac actgtctcac caggctggcc agaagcagca acaaaccgga gctcccaacc
     2761 agccacatga tcctggctgt gggcctcaat atcctagagc acacggtgcc gctaaactat
     2821 gagggtctgt agggtggtgt atgaaagtcc tgttgactaa ataatagttt cagttcatga
     2881 taggcttatg ggcatgtgac ctcatcctat gttgaggaga ttatgaatcc tgttgccttt
     2941 gaaatgagtt ggcactaaat gggtcattaa aagtgacact gtgtcaggaa aagacaaaac
     3001 atcagctctc aggaggagga agcctttatt tggtgcttgc cctgaaaaaa aaacaaaaaa
     3061 caaaacaaaa caaaacaaaa aaccccaaaa ccgaaaacaa aacaaaacaa aacaaaaaaa
     3121 actaacaaaa caaaacaaca aacaaacaaa aagctcaata gcacctgagt ttcgcaaaga
     3181 tggagttgct gcagtgtctt gaactaagag agcaaaagat cactgcttaa aaaaatgttg
     3241 agtttattct cacaaaagat ttctaaggat ttaggtaatg tgtctttaaa gctccaacaa
     3301 accatggaat caactgggaa ggaggcgaat tagcctttgt tgtgtggttg aaagcaaatt
     3361 agtgctttca gaacactggt aggggctgtt gaaacagaca tctattgttc aaagttttct
     3421 tttcccttca agaatggtat tttgttgaat tccaggaagc cagtttgact tcctctttag
     3481 aagaaggatt tctgagacac attcaagtga ttaaattcaa agcaaattgt caacatcaca
     3541 agggggatgc aatgttgcat ctttctttct ggttctttcc ttcactggtc tttaggttat
     3601 ataacaaaat ctcagatatc taccttacaa agtataatgt tcactaaatg aatgaggaaa
     3661 aagataccaa aggaaaaaag tgtagaggca cataataata atacaattta gggttaaaaa
     3721 aagtcttttg cctaaaaata atgcatagat tctaatgaca taattagttg gctgaaagga
     3781 tcttcctatt aattttgctt taaagtctaa tttttttccc cagttaatat ctcccaccta
     3841 ctattaaccc tccctgcata gcctctgcaa ttcactgaca gtatattcct ggtgccaggg
     3901 ctgtagactt cactttctct aaacgtgatg tgctcagtag aagtaagatg tgctaggaat
     3961 atgcacgaac atgctccctc tttaagtttc ctgagaattt cctgctaagc ccaggaggaa
     4021 gataactggg tggacctacc acagagaaat gaaagggatg gtctttaaat taaagatgag
     4081 actaagggct actttctcta cccatgtgcc tcatctccag gatccaattt aattaagttc
     4141 tgaagaccta accataaact ataaaaggtc cagggaattc ttgcaagcct agaagagtct
     4201 taaattataa aggcccaatt ctcttcactt taaaagaaac tttggaaatc catatcagat
     4261 ctgagttcaa ccacagacag taccttgcaa tctctcccag agttgcagag acagggtctc
     4321 aggcaaccct tgtaagtcat aataatgaag gtttgggctt gctccaagga gaagtggggt
     4381 ttgcttcaat cctggattat ctgaatcctc agagagctat ttcataatta gccagttctt
     4441 ctggggctca tcagctgaca ctggccactg accaacccca tatggatcaa gcttccatcc
     4501 ctccaacacc aaagaggagg tctcaaagct cttaagggga acgaaacttg tagagaaaaa
     4561 aaaaaggcag gtggcttgcc tttcacatgt ccctccttgt ggctctagga gtccccatgg
     4621 tgctatcaaa ccaacttgga cattattcaa gaagcacaac tcttcagtgg aattttctaa
     4681 gtgtttcaca tttgtatgaa tgtcaagaga tttccaagtg tgatttgcat ttttaccatt
     4741 ataagcaacc tgtgatggat caaagtcaga tgctataaat tatttgctac agaaaatgag
     4801 aacatagcca atgcccactg gctgttgaac atttcaaaac ttggttttta gcttattctc
     4861 aggagaatac gtgttgctta gaaatcataa aatgtcacct agtacaatgt attaaatgtt
     4921 tatattttga atgaaaaaaa aaagaataaa tgtcaacagg gagacttaaa aaaaaaaaaa
     4981 aaaa
//
