LOCUS       NM_032003               2602 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 5
            (Enpp5), transcript variant 1, mRNA.
ACCESSION   NM_032003
VERSION     NM_032003.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Takaya,K. and Kishi,K.
  TITLE     Regulation of ENPP5, a senescence-associated secretory phenotype
            factor, prevents skin aging
  JOURNAL   Biogerontology 25 (3), 529-542 (2024)
   PUBMED   38436793
  REMARK    GeneRIF: Regulation of ENPP5, a senescence-associated secretory
            phenotype factor, prevents skin aging.
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2602)
  AUTHORS   Kasowitz,S.D., Ma,J., Anderson,S.J., Leu,N.A., Xu,Y., Gregory,B.D.,
            Schultz,R.M. and Wang,P.J.
  TITLE     Nuclear m6A reader YTHDC1 regulates alternative polyadenylation and
            splicing during mouse oocyte development
  JOURNAL   PLoS Genet 14 (5), e1007412 (2018)
   PUBMED   29799838
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2602)
  AUTHORS   Gorelik,A., Randriamihaja,A., Illes,K. and Nagar,B.
  TITLE     A key tyrosine substitution restricts nucleotide hydrolysis by the
            ectoenzyme NPP5
  JOURNAL   FEBS J 284 (21), 3718-3726 (2017)
   PUBMED   28898552
REFERENCE   5  (bases 1 to 2602)
  AUTHORS   Liu,X., Jiao,Y., Cao,Y., Deng,N., Ma,Y., Hasty,K.A., Kang,A.,
            Chen,H., Stuart,J.M. and Gu,W.
  TITLE     Decreased expression levels of Ifi genes is associated to the
            increased resistance to spontaneous arthritis disease in mice
            deficiency of IL-1RA
  JOURNAL   BMC Immunol 17 (1), 25 (2016)
   PUBMED   27480124
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2602)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 2602)
  AUTHORS   Ohe,Y., Ohnishi,H., Okazawa,H., Tomizawa,K., Kobayashi,H., Okawa,K.
            and Matozaki,T.
  TITLE     Characterization of nucleotide pyrophosphatase-5 as an
            oligomannosidic glycoprotein in rat brain
  JOURNAL   Biochem Biophys Res Commun 308 (4), 719-725 (2003)
   PUBMED   12927778
REFERENCE   8  (bases 1 to 2602)
  AUTHORS   Gijsbers,R., Ceulemans,H., Stalmans,W. and Bollen,M.
  TITLE     Structural and catalytic similarities between nucleotide
            pyrophosphatases/phosphodiesterases and alkaline phosphatases
  JOURNAL   J Biol Chem 276 (2), 1361-1368 (2001)
   PUBMED   11027689
REFERENCE   9  (bases 1 to 2602)
  AUTHORS   Chambers,D.M. and Abbott,C.M.
  TITLE     Isolation and mapping of novel mouse brain cDNA clones containing
            trinucleotide repeats, and demonstration of novel alleles in
            recombinant inbred strains
  JOURNAL   Genome Res 6 (8), 715-723 (1996)
   PUBMED   8858346
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT030230.13, BY252780.1, AF233377.1 and BC089320.1.
            
            On Jul 21, 2016 this sequence version replaced NM_032003.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF233377.1, AK220510.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                CT030230.13        16370-16413
            45-159              BY252780.1         3-117
            160-2127            AF233377.1         93-2060
            2128-2602           BC089320.1         4088-4562
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 20.3 cM"
     gene            1..2602
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /note="ectonucleotide pyrophosphatase/phosphodiesterase 5"
                     /db_xref="GeneID:83965"
                     /db_xref="MGI:MGI:1933830"
     exon            1..181
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /inference="alignment:Splign:2.1.0"
     exon            182..1045
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..1650
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /EC_number="3.6.1.29"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; ectonucleotide
                     pyrophosphatase/phosphodiesterase family member 5"
                     /codon_start=1
                     /product="ectonucleotide pyrophosphatase/phosphodiesterase
                     family member 5 isoform 1 precursor"
                     /protein_id="NP_114392.1"
                     /db_xref="CCDS:CCDS28802.1"
                     /db_xref="GeneID:83965"
                     /db_xref="MGI:MGI:1933830"
                     /translation="MIPEFLLASCTLATLCHSAPFSLQPEEQKVLVVSFDGFRWDYLY
                     KVPTPHFHYIMKNGVHVNQVTNVFITKTYPNHYTLVTGLFAENHGIVANDMFDPILNK
                     SFSLEHMDIYDSKFWEEATPIWITNQRAGHASGAAMWPGADVKIHDSFPTYYLPYNES
                     VSFEDRVAKIIEWFTAKDPINLGFLYWEEPDDTGHDVGPDSPLMGSVISDVDHKLGYL
                     IKMLKRAKLWNNVNLIVTSDHGMTQCSKQRVIELDRYLDKEHYTLIDHSPVAAILPKE
                     GKFDEVYDALAGAHPNLTVYKKEEIPERWHYKHNDRVQPIVAVADEGWYILQNKSDDF
                     LLGNHGYDNALAEMHPIFLAHGPAFRKNFTKEAMNSTDLYSLLCHLLNLTALPHNGSF
                     WNVQDLLSSATPKPIPYTQSTTLLLGSDKPGEDEQEESYPYYIGVSLGSIIAMVFFVV
                     LIKHLIRSQVHTLQYRQVEVAQPLLQA"
     exon            1046..1222
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..2585
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2458
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /note="major polyA site"
     polyA_site      2568
                     /gene="Enpp5"
                     /gene_synonym="D17Abb1e; E-NPP 5"
                     /note="major polyA site"
ORIGIN      
        1 ctggtaacag tgacaagctg gcgggagcca atggcgttcc agcagcgggg cgggctgcag
       61 cgacgagtca ggtgctctgc gctgaggctg cgtgcaggtg agcgaacagg atcctacagt
      121 gccccttgtt tctgcctcct cttggtccca aacgttccag gacagatctg tctcagcgcg
      181 gattacctta tgctgagctg atcaaggtct tgaaaaatga ttccagagtt tctccttgcg
      241 tcttgcacac tggctactct atgccattcg gctccgtttt ctctacagcc agaagagcag
      301 aaagttctgg tagtttcttt tgatggattc cgctgggatt acttatataa agttccaacc
      361 cctcatttcc attatatcat gaaaaatggt gtccatgtga accaagtcac taatgttttc
      421 atcacaaaga cctaccctaa ccattacact ttggtcactg gtctctttgc agagaatcat
      481 gggattgttg caaatgacat gttcgaccct attctgaaca agtccttctc cttggagcac
      541 atggatattt atgattctaa gttttgggaa gaagccacac caatatggat cacaaaccag
      601 agggcagggc atgctagtgg cgcggccatg tggcccggag cagatgtgaa gatccacgat
      661 agtttcccga cctattatct accttacaat gagtctgttt cctttgaaga cagagttgcc
      721 aaaatcattg agtggtttac agccaaagat cccatcaatc tcggtttcct ctactgggaa
      781 gaacccgatg acacgggcca tgatgtggga cctgacagtc ctctcatggg gtctgttatt
      841 tctgatgttg accacaagtt agggtatctg ataaagatgc tgaaaagggc aaagttgtgg
      901 aataatgtta acctcatcgt cacgagcgac catgggatga cccagtgctc taagcaaagg
      961 gtgatagaac ttgaccgcta cctggacaaa gagcactaca ccttgattga tcactcacct
     1021 gtagctgcca tcctgccaaa agaaggtaaa tttgatgaag tctatgatgc gctggccggt
     1081 gcccatccta atctaacggt ctataaaaag gaggagatac cagaaagatg gcattacaag
     1141 cacaatgaca gagttcaacc aatcgtagct gtggccgatg aagggtggta tattttgcag
     1201 aataagtcag atgattttct gttaggcaac catggttatg ataatgcatt agcagaaatg
     1261 catccaatat ttttagccca cggtcctgcc ttcagaaaga atttcacaaa agaagccatg
     1321 aactccacag atctgtactc actgctctgc cacctcctca atctcaccgc cctgccacac
     1381 aatggatcat tctggaatgt ccaggacctt ctcagttcag caactcccaa gccaattcct
     1441 tacacacaga gtaccacact cctccttggg agtgacaaac caggggagga tgagcaagag
     1501 gaatcgtacc cttattacat aggtgtctct cttggcagca tcatagctat ggtattcttt
     1561 gtagttctca ttaagcattt aatacgcagt caagtgcata ctttacaata taggcaggtc
     1621 gaagttgctc aaccgttact ccaagcttag tgcttctttg acatggaatg gcagatcaaa
     1681 gtggagggtc cgaaattcta tcagcggtga cccttcagct tctcacaagc tagtcacaga
     1741 tatctgcata ggccatcaag cagtttaccc atacgcgggc ccagtcacag gtacatacac
     1801 gcacaccgac agatcgaaac actccctgcc tgcaaatgat caaagacgtt aagggtaccc
     1861 ctccacggct ttctctggct tagtaggtag atggtgctct gtctggggtt ggcacgtaca
     1921 atgtacatac ttaacaactt tgcactattg agagtaccta agtgctatcc tagtaggtac
     1981 tacttctctg acatgcactt tataaatgca tctagtaact tgattgaaca tgatatcctt
     2041 cttcatctct gtcaccaaat actagcattc catgtacaga gtgaaggaag tttctagcaa
     2101 ttcccaaatg atgtagaatt cttgtctcct taaatggaag gaggaggagg aggaggaaga
     2161 ggaggaggag gaggaggagg aagtaaaggt gttgaaagtc agcaggacca agtttgtagc
     2221 ctgcatctgg atagatgctt ttccagcagc aggtcgcact gtggacattt ctagttatgc
     2281 cccccaccca cacctcagtg accatcgttt tcatttgctt cttccctcag ctagcttcca
     2341 gcactgaaag gtgaattcta aagatcttat ctctatacat tagtattgtt ccctgtggat
     2401 gagtctagtc ctgactccgt gatgaggatg tcatgccatg cacatgagtc ttcctttctg
     2461 tgtagaatag cggtggcctg aaaagcgggg ggccagacac catctctgct gtgttttttt
     2521 tttcctgcct gtcatgattt gcattattaa tcatattaaa aagatcaagt gaaatttaaa
     2581 aaaaaaaaaa aaaaaaaaaa aa
//
