LOCUS       NM_008813               6791 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1
            (Enpp1), transcript variant 2, mRNA.
ACCESSION   NM_008813
VERSION     NM_008813.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Sun,F., Yang,Y., Jia,L., Dong,Q.Q., Hu,W., Tao,H., Lu,C. and
            Yang,J.J.
  TITLE     TET3 boosts hepatocyte autophagy and impairs non-alcoholic fatty
            liver disease by increasing ENPP1 promoter hypomethylation
  JOURNAL   Free Radic Biol Med 218, 166-177 (2024)
   PUBMED   38582229
  REMARK    GeneRIF: TET3 boosts hepatocyte autophagy and impairs non-alcoholic
            fatty liver disease by increasing ENPP1 promoter hypomethylation.
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Arima,T., Sugimoto,K., Taniwaki,T., Maeda,K., Shibata,Y.,
            Tateyama,M., Karasugi,T., Tokunaga,T., Sueyoshi,T., Hisanaga,S.,
            Masuda,T., Uehara,Y., Yugami,M., Matsushita,K., Yonemitsu,R.,
            Kawakami,J., Yoshimura,N., Tanimura,S., Kato,H., Ito,N., Inoue,K.,
            Bando,K., Nakamura,T. and Miyamoto,T.
  TITLE     Cartilage tissues regulate systemic aging via ectonucleotide
            pyrophosphatase/phosphodiesterase 1 in mice
  JOURNAL   J Biol Chem 300 (1), 105512 (2024)
   PUBMED   38042486
  REMARK    GeneRIF: Cartilage tissues regulate systemic aging via
            ectonucleotide pyrophosphatase/phosphodiesterase 1 in mice.
REFERENCE   3  (bases 1 to 6791)
  AUTHORS   Wang,S., Bohnert,V., Joseph,A.J., Sudaryo,V., Skariah,G.,
            Swinderman,J.T., Yu,F.B., Subramanyam,V., Wolf,D.M., Lyu,X.,
            Gilbert,L.A., Van't Veer,L.J., Goodarzi,H. and Li,L.
  TITLE     ENPP1 is an innate immune checkpoint of the anticancer cGAMP-STING
            pathway in breast cancer
  JOURNAL   Proc Natl Acad Sci U S A 120 (52), e2313693120 (2023)
   PUBMED   38117852
REFERENCE   4  (bases 1 to 6791)
  AUTHORS   Attalla,S.S., Boucher,J., Proud,H., Taifour,T., Zuo,D.,
            Sanguin-Gendreau,V., Ling,C., Johnson,G., Li,V., Luo,R.B.,
            Kuasne,H., Papavasiliou,V., Walsh,L.A., Barok,M., Joensuu,H.,
            Park,M., Roux,P.P. and Muller,W.J.
  TITLE     HER2Delta16 Engages ENPP1 to Promote an Immune-Cold
            Microenvironment in Breast Cancer
  JOURNAL   Cancer Immunol Res 11 (9), 1184-1202 (2023)
   PUBMED   37311021
  REMARK    GeneRIF: HER2Delta16 Engages ENPP1 to Promote an Immune-Cold
            Microenvironment in Breast Cancer.
REFERENCE   5  (bases 1 to 6791)
  AUTHORS   Gao,Z., Wang,Q., Guo,K., Li,X. and Huang,Y.
  TITLE     Enpp1 deficiency caused chondrocyte apoptosis by inhibiting AMPK
            signaling pathway
  JOURNAL   J Orthop Surg Res 18 (1), 462 (2023)
   PUBMED   37370114
  REMARK    GeneRIF: Enpp1 deficiency caused chondrocyte apoptosis by
            inhibiting AMPK signaling pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6791)
  AUTHORS   Buckley,M.F. and Goding,J.W.
  TITLE     Plasma cell membrane glycoprotein gene Pca-1 (alkaline
            phosphodiesterase I) is linked to the proto-oncogene Myb on mouse
            chromosome 10
  JOURNAL   Immunogenetics 36 (3), 199-201 (1992)
   PUBMED   1351877
REFERENCE   7  (bases 1 to 6791)
  AUTHORS   Rebbe,N.F., Tong,B.D., Finley,E.M. and Hickman,S.
  TITLE     Identification of nucleotide pyrophosphatase/alkaline
            phosphodiesterase I activity associated with the mouse plasma cell
            differentiation antigen PC-1
  JOURNAL   Proc Natl Acad Sci U S A 88 (12), 5192-5196 (1991)
   PUBMED   1647027
REFERENCE   8  (bases 1 to 6791)
  AUTHORS   Buckley,M.F., Loveland,K.A., McKinstry,W.J., Garson,O.M. and
            Goding,J.W.
  TITLE     Plasma cell membrane glycoprotein PC-1. cDNA cloning of the human
            molecule, amino acid sequence, and chromosomal location
  JOURNAL   J Biol Chem 265 (29), 17506-17511 (1990)
   PUBMED   2211644
REFERENCE   9  (bases 1 to 6791)
  AUTHORS   van Driel,I.R. and Goding,J.W.
  TITLE     Plasma cell membrane glycoprotein PC-1. Primary structure deduced
            from cDNA clones
  JOURNAL   J Biol Chem 262 (10), 4882-4887 (1987)
   PUBMED   3104326
REFERENCE   10 (bases 1 to 6791)
  AUTHORS   Dumont,F.J., Habbersett,R.C., Coker,L.Z., Nichols,E.A. and
            Treffinger,J.A.
  TITLE     High level expression of the plasma cell antigen PC.1 on the T-cell
            subset expanding in MRL/MpJ-lpr/lpr mice: detection with a
            xenogeneic monoclonal antibody and alloantisera
  JOURNAL   Cell Immunol 96 (2), 327-337 (1985)
   PUBMED   2943419
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC158616.8, AK164619.1,
            AK029600.1, AC099695.15 and AI428932.1.
            
            On May 5, 2015 this sequence version replaced NM_008813.3.
            
            Summary: This gene encodes a member of the nucleoside
            pyrophosphatase/phosphodiesterase family of enzymes that catalyzes
            the hydrolysis of pyrophosphate and phosphodiester bonds in
            nucleotide triphosphates and oligonucleotides, respectively, to
            generate nucleoside 5'-monophosphates. The encoded protein is a
            type II transmembrane glycoprotein that negatively regulates bone
            mineralization. Mice harboring a nonsense mutation in this gene,
            termed tiptoe walking (ttw), exhibit ectopic ossification of the
            spinal ligaments. The encoded protein binds to the insulin
            receptor, inhibits downstream signaling events and induces insulin
            resistance and glucose tolerance. This gene is located adjacent to
            a paralog on chromosome 10. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088857.1, AK145233.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AC158616.8         128254-128415
            163-257             AK164619.1         3-97
            258-2340            AK029600.1         2-2084
            2341-2341           AC099695.15        110594-110594       c
            2342-2906           AK029600.1         2086-2650
            2907-6384           AC099695.15        100807-104284       c
            6385-6791           AI428932.1         1-407               c
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 12.26 cM"
     gene            1..6791
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /note="ectonucleotide pyrophosphatase/phosphodiesterase 1"
                     /db_xref="GeneID:18605"
                     /db_xref="MGI:MGI:97370"
     exon            1..354
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..2886
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /EC_number="3.1.4.1"
                     /EC_number="3.6.1.9"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ectonucleotide pyrophosphatase/phosphodiesterase family
                     member 1; lymphocyte antigen 41; plasma-cell membrane
                     glycoprotein PC-1; tiptoe walking; phosphodiesterase
                     I/nucleotide pyrophosphatase 1; NPPase; nucleotide
                     diphosphatase; alkaline phosphodiesterase I"
                     /codon_start=1
                     /product="ectonucleotide pyrophosphatase/phosphodiesterase
                     family member 1 isoform 2"
                     /protein_id="NP_032839.3"
                     /db_xref="CCDS:CCDS35870.1"
                     /db_xref="GeneID:18605"
                     /db_xref="MGI:MGI:97370"
                     /translation="MERDGDQAGHGPRHGSAGNGRELESPAAASLLAPMDLGEEPLEK
                     AERARPAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNC
                     RCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCI
                     NYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVIS
                     KLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKE
                     KFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILA
                     VLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDL
                     GLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYS
                     FNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSE
                     RKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPN
                     NGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLN
                     LTTEDDDIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQ
                     FSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTS
                     NIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSR
                     VIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESS
                     WVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED"
     exon            355..427
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            428..544
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            545..670
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            671..731
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            732..829
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            830..909
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1029
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1139
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1205
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1278
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1387
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1519
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1551
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1679
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1749
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1837
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..2004
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            2005..2056
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2208
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            2209..2338
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2419
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            2420..2552
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2715
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..6778
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6079..6084
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /note="hexamer: AATAAA"
     polyA_site      6099
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /note="major polyA site"
     regulatory      6761..6766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /note="hexamer: AATAAT"
     polyA_site      6778
                     /gene="Enpp1"
                     /gene_synonym="4833416E15Rik; CD203c; E-NPP 1; E-NPP1;
                     Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw;
                     twy"
                     /note="major polyA site"
ORIGIN      
        1 cgcgcccagg gcccgaaatc agacaggaag ccaaataccc ggggcgttga gcggctcggg
       61 ccggagcgct ggccgagggc tgggagccaa gggtctggcg ggggcggcgc ggggcggggc
      121 ggggctgcga gcctattaaa gctcggcccg cgggggcaga gcggggcgat ggagcgcgac
      181 ggcgaccagg cagggcacgg gccccggcac gggtcagcgg gaaacggtcg cgagctcgag
      241 tcaccagccg cggcttcgct gctcgcgccc atggacctag gagaggagcc gctggagaag
      301 gcggagcgtg cccgccctgc caaagacccc aacacctaca aagtgctgtc gctggttttg
      361 tcagtatgtg tgctaacaac aattcttggt tgtatatttg ggttgaaacc aagctgcgcc
      421 aaagaagtaa aaagttgcaa aggccgctgc tttgaaagga cgttcagcaa ctgtcgctgt
      481 gatgctgcct gtgtcagcct tgggaactgc tgtctggatt tccaggagac ctgtgtggaa
      541 ccaacacata tatggacttg caacaaattc aggtgcggtg agaagaggct gtccaggttc
      601 gtgtgctcct gtgcagacga ctgcaaaacc cacaatgact gttgcatcaa ctacagttct
      661 gtgtgccaag ataagaagag ttgggtagaa gaaacatgtg aaagcatcga taccccggag
      721 tgtccagcag agtttgaatc accccctact ctcttgtttt ctttggatgg attcagagct
      781 gagtatttgc acacctgggg tggacttctt cctgtcatta gcaagctgaa aaactgtgga
      841 acatacacta aaaacatgag gcctatgtac cctaccaaga cgtttcccaa tcattacagc
      901 atcgtcacag gactttatcc ggaatcccat ggcataattg ataacaagat gtatgatccc
      961 aaaatgaatg cttctttctc gcttaaaagt aaagagaaat tcaacccttt gtggtacaaa
     1021 ggacagccga tttgggtgac cgctaatcat caggaggtca agtccggcac atacttttgg
     1081 ccaggatcag atgtggagat tgacgggatt ctaccagata tctacaaagt gtataatggg
     1141 tcagtaccat ttgaagaaag gattttagct gttcttgagt ggctgcagct tcctagccat
     1201 gaaagaccac acttttacac tctgtattta gaagaaccag attcttcagg gcattcacat
     1261 ggaccagtca gcagcgaggt catcaaggcc ttgcagaagg ttgaccgcct ggtcggcatg
     1321 ctgatggacg gcctgaagga cctgggcttg gataaatgcc tgaacctcat cctcatttca
     1381 gatcacggca tggaacaagg cagctgtaag aagtatgtgt acctgaataa gtacttgggg
     1441 gatgtgaaca atgtgaaagt tgtgtatgga cctgctgctc ggttgagacc cactgatgtt
     1501 ccagagacat actattcatt taactatgaa gcccttgcaa aaaatctttc ttgccgggag
     1561 ccaaaccagc atttccggcc ttacctgaaa cccttcttac ccaagcgctt acacttcgct
     1621 aaaagtgacc ggattgagcc cctgaccttc tacctggacc ctcagtggca acttgcgttg
     1681 aatccatcag agaggaaata ttgtggaagt ggatttcatg gctctgacaa cttgttttca
     1741 aacatgcaag ctctttttat tggctatgga cctgccttca agcatggtgc tgaagttgac
     1801 tcctttgaaa atattgaagt ctataactta atgtgtgact tactgggttt gatcccagct
     1861 cccaataatg gaagtcatgg cagccttaac caccttctaa agaaacccat ttacaaccca
     1921 agtcatccca aagaagaggg cttcctgtct cagtgtccaa tcaaatcaac atccaatgac
     1981 ctcggctgca catgtgaccc ttggattgtg ccaatcaagg actttgagaa acagcttaat
     2041 ctgaccacag aagatgatga catttaccat atgactgtac cctacgggag gccccggatt
     2101 ctactgaagc agcaccgcgt ctgtctactc cagcagcaac agttcttgac cggatacagc
     2161 ctggacctcc tcatgcccct ctgggcgtct tacaccttcc tcagtaatga ccaattctcc
     2221 agagatgact tttccaactg tctgtaccag gatcttcgga ttccccttag tcctgtgcat
     2281 aaatgttcct attacaaaag caattctaag ctaagttatg ggttcctcac gccaccgaga
     2341 ctaaatagag tttcaaatca catatactct gaagcgctgc ttacatctaa tatagtgcca
     2401 atgtatcaga gttttcaagt tatatggcac taccttcatg acaccctact gcaaaggtat
     2461 gcccatgaaa ggaacggcat caatgttgtc agcggtcccg tgtttgactt tgattatgac
     2521 ggacgctatg attccttaga gatcctgaaa caaaacagca gagtcatccg aagccaggaa
     2581 attctgattc cgactcactt cttcattgtg ctcaccagct gcaagcagct gtctgagact
     2641 cccttggagt gctccgcctt agagtcctca gcttacatac tgcctcatag gcctgataac
     2701 attgagagct gtacgcatgg gaagcgggag tcttcatggg tcgaggagtt gctgaccttg
     2761 cacagagctc gggtcacaga cgtggaactc atcactggcc tcagcttcta ccaggaccga
     2821 caagagtcag tttcagaact gctgaggttg aaaacacatt tgccaatctt cagccaagaa
     2881 gactgattgt ttttttatta aaaaacaaaa gaaaacaaaa caaacaccat agatcctttt
     2941 gaaagagtct tatatttgat acagtcctct acacttttgc attgtttgga aacggtcgag
     3001 tggagttaca actgggactc cctgtgtggt gtcgatgtcc ctggctgggt tgtgtgacga
     3061 ctcagcacat ctgcagagtg ttcctgtcct gtgccatgca gacttcctgt ctaagaatta
     3121 gatgtgtcac taacgcacgg ggagtaaaga cacttcacct cacacctgga agtgttctta
     3181 agggacgagg ggcagtgtac gtggtctggg gacctgatgt tggaatccta ttgttgttaa
     3241 taaaactgag taaaggactg gggtagctca tgtcccattt taaaaaaaat ggaaaatggt
     3301 tctgtattga ttttcacttg gagtttcagt tttagctaaa tgaggcccca tttctatttt
     3361 ttctgtctcc tccaataacc aaagtcttgt gcttttaaaa caaattgtga ttgggtctct
     3421 tcaggtttag tggctttcca tcaaaaaact ggacttttga tggaaaattt gtgagttgag
     3481 gatatctagc ttttgtaaaa atctgactct atgtttatac acacacgcac acatacacat
     3541 gtacacacgc acacacatgt gcacatgcat gtatgcatgc acacacacat gtaacacatg
     3601 tgcacacaca tgtacacaca tacacatgta acacacccac acacgtgcat gctcttgaat
     3661 atgcacatgc acacacatat gcacatgcac atcccagtcc tctttactag aaataaatga
     3721 agactgaaaa gagccctgag catggttctc tcaggccatt aaaattgggc tttgtttgga
     3781 tgaaccccga gagaactcat tggcacttgc aaagtgactt ttttttccac tgtgaagagt
     3841 gttttctctg cacaagagag cccccctccc ccacagtgat gggagaagag ctttgcctgg
     3901 cttagttctt cagtttgaag ctgtgtagat ctgaagagaa ttcgtcactc atcacttaac
     3961 atcttcactg tgcacacgac aaatgacaca gtgatgtaac ttcccctcac cgacattgcc
     4021 actgacattg atgtaataac ttagaaggtg ttgttttgtg ttacccatag cctctggcat
     4081 gcatatcttt cagtctgttc tttccaagtt tgctacttcc tgccagtctg tctaaggttt
     4141 tgtttggttt ttatgctacc ttttatccat tctgttgttc ccagtaggtt tatatatagg
     4201 ctactgtttt atttgtaaaa tgttatttgt gttttgcttc tttatgtctt ctatttgctg
     4261 aaatttttta ttgcttccat tgagtatcaa tttcttcatt tgtttcatgt gcatttgcgg
     4321 ttgctggttg gaacattttt acgatgctgt tgtacagtgc tcgttggtac atcctcactt
     4381 cctctgtcaa tgtttgtttc tatgtgtagt gggtttctct tgtcttcaac atattttttt
     4441 tgcttctcat gattgtttta aattaaaacc tggatatttt aggcattcta tcttagcatt
     4501 ctgcatcttc ggtaaatctc ctctgacatc atccctgcag aaatactggg gactcggcct
     4561 ccttcttact ctctgggaga tgaagtattt cacctcctcc attgatatta ggatgagagg
     4621 aggtaagcgg agtggggaat tcagatctct gttcgttcat tgctgatgcc tcccgtgctg
     4681 ggatgtgaac agtaccactt actgctcctg gcgggtctcc agtgatgcag gcatcctgga
     4741 gaaagcttca tcaccactgg atagagatga ccctctcagg ctactcagcc tccactggca
     4801 ctgccctggc agcggagctg gagagctggg tatggctttt acagcttgcc agccgtagaa
     4861 gtgtaggctc cacccccttg gctggtgtgg gagggtcttt tttgtgtttc acagaagcaa
     4921 agtagattct ggtgagctcc gccctttcct gccctttgga tggggagagt aagccgtgtt
     4981 ttccactccc tgccctgtca ttctggcatc ttcatgctcc ttcatcaagt ctgagggaaa
     5041 tgagctaaaa ggaaaacttg ggacacggaa cgttactctt gagttctgag gtacttagcc
     5101 tgcctatgtg gtactcagtc ctcctgggtt tgtttttatg tataatataa aggctttgtc
     5161 acccaattag cagtatatgt gcttagttac acgacccact tggcctgaag catcctagtg
     5221 gggcttcatt aaatggatag gctcccgttc tctgagctag acactgaagt cgtaaagagg
     5281 agctggttac aattcctata atcccgagga aacaggcagc ggtggcagaa gtgtgagtgg
     5341 gttcctaagg agcaggaaat gtcactgaac catgaaagat atactgggta cttaattcag
     5401 agtccatagt tcttagggtc tacctgtagg tgatggtggc tttgtcctac tttttctgac
     5461 aaggaccaat atgcaaaaag aacccccccc cccactttaa atcctaaatc tgacttctgt
     5521 accttgggag tccatacttc ttaaggaagg ttccttagaa aatgacccgt gtggtcagta
     5581 ccttcattgc tgagaaagaa ccaagcagga gtttttaaac aatgttttca agaacaggac
     5641 cacagtggga agaaggtgaa ggctcagagt ctgtagggaa aggatatctg aagcgtttaa
     5701 ggactgttta aatggaggct gtggggagcc atgtgttcag gagatgtgat gaggtgaggc
     5761 aggtgactgc ccgcagacgt ttggtcctcc ttgccctttc ccctgttatt agccagggta
     5821 acttctgtcc tgtgggcaca tacttatctg ccagaggaac tcacacttga actttgaaac
     5881 aattaagctg attacccttc gtgtagaagc ttgtagcagg aagagagtga tgtaaacgca
     5941 ttcctaagcg aaaattttag catggttaca aagcagggac atttgggggc cggttttggt
     6001 cagtctaaat attgtaagac aaccaaaaat tattctacag gatgtaggtg tgtctatatt
     6061 gagaaatgtc aatttcttaa taaaactcta ttatattgag tcttggcagc ttgcctatgt
     6121 ggctgtgtgt atgtgtgtgt tggggtgggg gaggggtgga gcttcctaca gaaaaatgat
     6181 accaagtcca ggcattgttg acattaaacc gcagctgcgt tgttggaggg agaaaaggaa
     6241 ttaaagttca cttcaggttc gcagagcaaa cagtgccagc ctgcagcctc tgtcctcttt
     6301 ggggacaagg gcatgtgact gtcatttcag agtcagaaag agtctgaaat tagaggaaag
     6361 gtgctaactt gtgacaacta tagacatttt cagtgtgaag tgtttgactg tattccttcc
     6421 tctgtaagta atggttctag gaaagtgctg ctttctagaa attatatcta tcatcagaat
     6481 ctacaaacac atgaaatatc atgaaatgtg atgtcttagt tccgtacaaa tggcagaatc
     6541 ggcctgaatg acacagaaat tgacaaagca aggctcagct ttttttctct tgtaactttg
     6601 catagtattc tcagcgtctt cctgttttgt gaaaagatct cttaccagtt tacaatgcaa
     6661 ctgacctgta ctttactgtg cttctcaatg ctcttccagc ttaactctat gttgaagcat
     6721 aaactatctg gtcaggggat aatttcaagg ttttaataaa aataatgctt tgacttaaaa
     6781 aaaaaaaaaa a
//
