LOCUS       NM_001271447            3977 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ecto-NOX disulfide-thiol exchanger 2 (Enox2),
            transcript variant 1, mRNA.
ACCESSION   NM_001271447
VERSION     NM_001271447.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Kam,N.W., Laczka,O., Li,X., Wilkinson,J., Hung,D., Lai,S.P.H.,
            Wu,K.C., Tsao,S.W., Dai,W., Che,C.M., Lee,V.H. and Kwong,D.L.
  TITLE     ENOX2 inhibition enhances infiltration of effector memory T-cell
            and mediates response to chemotherapy in immune-quiescent
            nasopharyngeal carcinoma
  JOURNAL   J Adv Res 56, 69-86 (2024)
   PUBMED   37061217
  REMARK    GeneRIF: ENOX2 inhibition enhances infiltration of effector memory
            T-cell and mediates response to chemotherapy in immune-quiescent
            nasopharyngeal carcinoma.
REFERENCE   3  (bases 1 to 3977)
  AUTHORS   Tang,X., Kane,V.D., Morre,D.M. and Morre,D.J.
  TITLE     hnRNP F directs formation of an exon 4 minus variant of
            tumor-associated NADH oxidase (ENOX2)
  JOURNAL   Mol Cell Biochem 357 (1-2), 55-63 (2011)
   PUBMED   21625959
  REMARK    GeneRIF: this result suggests that hnRNP F directs formation of the
            exon 4 minus variant of ENOX2
REFERENCE   4  (bases 1 to 3977)
  AUTHORS   Yagiz,K., Snyder,P.W., Morre,D.J. and Morre,D.M.
  TITLE     Cell size increased in tissues from transgenic mice overexpressing
            a cell surface growth-related and cancer-specific hydroquinone
            oxidase, tNOX, with protein disulfide-thiol interchange activity
  JOURNAL   J Cell Biochem 105 (6), 1437-1442 (2008)
   PUBMED   18980249
  REMARK    GeneRIF: The tissue expression of tNOX mRNA was greatest in heart,
            lung and liver. When these tissues were analyzed for cell size.
REFERENCE   5  (bases 1 to 3977)
  AUTHORS   Yagiz,K., Wu,L.Y., Kuntz,C.P., James Morre,D. and Morre,D.M.
  TITLE     Mouse embryonic fibroblast cells from transgenic mice
            overexpressing tNOX exhibit an altered growth and drug response
            phenotype
  JOURNAL   J Cell Biochem 101 (2), 295-306 (2007)
   PUBMED   17115410
  REMARK    GeneRIF: Data show that growth of transgenic mouse embryonic
            fibroblasts overexpressing tNOX is inhibited by low concentrations
            of the anticarcinogenic tNOX inhibitors epigallocatechin-3-gallate
            and phenoxodiol.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK041822.1, AK137736.1 and BX294194.10.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Variants 1, 2, 3,
            and 4 encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK137736.1, AK139897.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AK041822.1         2-31
            31-2170             AK137736.1         2-2141
            2171-3977           BX294194.10        8441-10247          c
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 25.79 cM"
     gene            1..3977
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /note="ecto-NOX disulfide-thiol exchanger 2"
                     /db_xref="GeneID:209224"
                     /db_xref="MGI:MGI:2384799"
     exon            1..142
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            143..202
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            203..346
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            347..481
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     CDS             385..2181
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /note="isoform a is encoded by transcript variant 1;
                     cytosolic ovarian carcinoma antigen 1; APK1 antigen;
                     tumor-associated hydroquinone oxidase"
                     /codon_start=1
                     /product="ecto-NOX disulfide-thiol exchanger 2 isoform a"
                     /protein_id="NP_001258376.1"
                     /db_xref="CCDS:CCDS30115.1"
                     /db_xref="GeneID:209224"
                     /db_xref="MGI:MGI:2384799"
                     /translation="MTLPVSDPAAWATAMNNLGMAPLGIAGQPILPDFDPALGMMTGI
                     PPITPMMPGLGIVPPPIPPDMPVAKEIIHCKSCTLFPPNPNLPPPATRERPPGCKTVF
                     VGGLPENGTEQIIVEVFEQCGEIIAIRKSKKNFCHIRFAEEYMVDKALYLSGYRIRLG
                     SSTDKKDTGRLHVDFAQARDDLYEWECKQRMLAREERHRRRMEEERMRPPSPPPVVHY
                     SDHECSIVAEKLKDDSKFSEAVQTLLTWIERGEVNRRSANHFYSMIQSANSHVRRLVN
                     EKATHEKEMEEAKEKFKQALSGILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISVWC
                     KQAEEIRNIHNDELMGIRREEEMEMSDDEIEETTETKETEESALVSQAEALKEENDSL
                     RWQLDAYRNEVELLKQEQGKAHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKE
                     AELDRIKDDNLQVEQLLENFHEKQENCGSRLCASSQEGEQPLEKTAVSNPVKSEREAL
                     LVGIISTFLHVHPFGASIEYICSYLNRLDNKASYQIPSKLTTSPLLPISTSDVESLMS
                     RLQHTFRQEMTGVGASLEKRWKFCGFEGLKLT"
     exon            482..637
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            638..844
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            845..1078
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1291
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1398
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1513
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1695
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1803
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1915
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2001
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2049
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..3977
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2212..2217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /note="hexamer: AATAAA"
     polyA_site      2236
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /note="major polyA site"
     regulatory      3164..3169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /note="hexamer: AATAAA"
     polyA_site      3169
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /note="major polyA site"
     regulatory      3820..3825
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
                     /note="hexamer: AATAAA"
     polyA_site      3842
                     /gene="Enox2"
                     /gene_synonym="APK1; Cova1; tNOX"
ORIGIN      
        1 atctagtaga aggtgcacgg ttgggaagac agttgtgggg cacaccccac cctgagggca
       61 ctccgggtcc aggaacttcc gagcgggtcc gattttccgc ggctgtttaa ggagtagcgc
      121 ttcggtagcc ggggagctgg aggggaacct gatttgagat caattcttgg agaacacaaa
      181 gaagtgtggt ggtatgttcc aggattgaac cagctgccct ttgaccctgc caacaacaac
      241 gaacccctgc agtttggtag tgccagtggc cctctggttc cagaaggcct cattgagaat
      301 ggaggggaat caagcaagaa aagaaagaga acaaatcctc cttcagctga taatactaga
      361 actctgaatg tagattctgc tgcaatgacg ctgcctgtgt ctgatccagc tgcatgggcc
      421 acagcaatga ataatcttgg aatggctcca ctgggaattg ctggacaacc aattttacct
      481 gacttcgatc ctgcccttgg gatgatgact ggaataccac caataactcc catgatgccg
      541 ggtttgggca tagtcccgcc accgattcct ccagatatgc cggtagcaaa ggagatcata
      601 cactgcaaaa gctgcacgct cttccctccc aacccaaatc ttccaccacc tgcaacacga
      661 gaaaggccac caggctgtaa gacagtgttt gtgggtggcc tgcctgaaaa tgggacagag
      721 cagatcattg tggaagtgtt tgaacagtgt ggagagatta ttgctatccg gaagagcaaa
      781 aagaacttct gtcacattcg ctttgctgag gaatacatgg tggacaaagc cctctatctg
      841 tctggttacc gaattcgtct gggctctagt actgacaaga aggacacagg ccggctccat
      901 gttgactttg cccaggctcg ggatgactta tatgagtggg agtgtaaaca gcgtatgcta
      961 gccagagagg agcggcaccg tagaaggatg gaagaagaaa gaatgcgtcc accatcccca
     1021 cctccagtgg tccactattc agatcatgaa tgcagcattg ttgctgaaaa actcaaagat
     1081 gattccaaat tctcggaagc tgtgcagacc ttgctcacct ggattgagag gggggaagtg
     1141 aaccgccgca gtgccaacca cttctactcc atgatccagt cagccaacag ccatgtccgc
     1201 cgcctggtaa atgagaaagc tacccatgag aaagagatgg aagaagcaaa agagaagttc
     1261 aagcaggccc tttctggaat tctcattcaa tttgagcaga tagtagctgt gtaccattcc
     1321 gcttccaaac agaaggcatg ggaccacttc acaaaagcac aacgtaaaaa catcagtgtt
     1381 tggtgcaaac aagctgagga aattcgcaac attcataatg atgaattaat gggaatcaga
     1441 agagaagaag aaatggaaat gtctgatgat gaaatagaag agacaacaga aacaaaagaa
     1501 acggaggaat cagccttagt gtcacaggca gaagctctga aggaggaaaa tgatagcctc
     1561 cgctggcagc tggacgcata ccggaatgag gtagaactgc tcaaacagga acaaggcaaa
     1621 gcccacaggg aggatgaccc aaacaaggaa cagcagctga aacttctgca gcaagcccta
     1681 caagggatgc aacagcatct gctcaaagtc caagaggaat acaaaaagaa agaagctgag
     1741 cttgaccgaa tcaaagacga caatttacag gtagaacaac tgttggaaaa ttttcatgaa
     1801 aagcaggaaa actgtggttc tagactgtgt gcatcaagcc aagaaggtga gcaacctctt
     1861 gagaagaccg cagtcagcaa tcctgtcaaa tctgaacgtg aggcactgct agtggggatc
     1921 atctccacat tccttcacgt ccacccattt ggagccagca ttgaatacat ctgttcctac
     1981 ttgaatcgtc tcgataataa ggcaagctac caaataccca gcaaattaac cacttctcca
     2041 ctcttaccga tcagcaccag tgatgtggaa tctctcatga gtagactcca gcataccttc
     2101 agacaggaaa tgactggagt tggagccagc ctggagaaga gatggaaatt ctgtggtttt
     2161 gagggcttga agctgacctg aagctctttg cctagaatta gagatcctga aaataaatgt
     2221 gtgcttgaca agcttaggat gtgagttttg tcctcagtgg ttttcaagtg gacattgctt
     2281 tcaatttgtc agttcattcc tgaatctctt tcaagttaaa ataaggatcc tagaatagcg
     2341 tctcaagcta caggtcctaa aaagaaattg cctcaaacct caagtgctgt aactttctcc
     2401 tatttctggc aattggttga cttgtcttat aagtaatagt gaaaaattcc tgagactaaa
     2461 gcgtattgga gtgttttcag tgtctgtact actgctgtag accttgtact ttttcaacgc
     2521 tgttagctgc agtgttttga tggtttgtat gtgctctgtg tttccagatt tctcttcacg
     2581 ggttgctact ggtaaaagga gtgagaggga tgctaggaac cccctgtaac tgccattgtc
     2641 tttctcatct acagtaaaca ctaaaggaat aacccatctg tgtcttctcc aaggtgcttc
     2701 accagttcac ctttgaaata aaaaagcatg aactgtggct ttttgtaaaa atactgtgaa
     2761 ccaaaagttg acaaacgttc caaagttatt ttctctaata gataaaaggt ctatttcaga
     2821 attttaaaac aggaaatcta gatggattca tgggaagtct ccagtatgac tactgtgttt
     2881 gtgcttttca gaactacttg taaatagtct gctttacaaa gagggcatgt ttagttttct
     2941 atgaattaaa atgcagtcag gtgtgggcac atgtgcacac acattggcag aagggattta
     3001 ttttgatagt cctttcctct ggccttccta ctgtctgttg gtcccatttt ttcctgctgt
     3061 tagtgtgctg aaatgcaaac tgtgtattgc tgtaaatact gtttacttca atgctggatg
     3121 tttgtaaaga actgatgtga gtattgaaag taaagaaata atgaataaat gatggactgg
     3181 gctgtgaggc ttcctgcaga tgcgtcagct ccacccagaa tgcttgactg acagggactc
     3241 ctctggttcc ctcataccca cctgtacatt gtaatggcct caccacgtgt aaatggacta
     3301 ttcaggaagt cctgcttggt aggaaaagaa aagaaagggt agacttggat ggaggctgtt
     3361 cctcgaaagt atagatggtc tttattattt tttgtggtgg taatggtggt acttttaatt
     3421 acacaatcat gctctccctt gagctctgtt gaaggtaaga aaatacactg aatgaatata
     3481 gccagtaaga gtatttcctg aaaatacttt gcaaatgaga tgtgatcagt atgcaaagat
     3541 gtatttttaa aatataaata ttccctttta tagctcagct ctttgttccg ttttgaatgg
     3601 cttgcttcct gcgtgataga tccttagatc atctcaggtc ttctctctga gttcccagca
     3661 tgttggaaga agatgccaag cttgcttggc aactagaaag aacattccac aggaaaccat
     3721 tcctgctgaa agttgacgtg cctcttagca ccccactgtg gccccagcag gaacctagag
     3781 ttatttttac actagcttct ttgctggagt atcttcctca ataaaggctg cttctctgga
     3841 aagctttgat tcttcagtct cagaggctcc taaagtatgt tcttttcgac aatatctgta
     3901 aaatcaagag aaatttctcc agtcgtcact tatttcttaa ataggttaaa taaatttcaa
     3961 ggcaacagct gccatac
//
