LOCUS       NM_172813               3549 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ecto-NOX disulfide-thiol exchanger 1 (Enox1),
            transcript variant 2, mRNA.
ACCESSION   NM_172813
VERSION     NM_172813.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Allegre,N., Chauveau,S., Dennis,C., Renaud,Y., Meistermann,D.,
            Estrella,L.V., Pouchin,P., Cohen-Tannoudji,M., David,L. and
            Chazaud,C.
  TITLE     NANOG initiates epiblast fate through the coordination of
            pluripotency genes expression
  JOURNAL   Nat Commun 13 (1), 3550 (2022)
   PUBMED   35729116
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3549)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3549)
  AUTHORS   Smith,C.A., Mont,S., Traver,G., Sekhar,K.R., Crooks,P.A. and
            Freeman,M.L.
  TITLE     Targeting Enox1 in tumor stroma increases the efficacy of
            fractionated radiotherapy
  JOURNAL   Oncotarget 7 (47), 77926-77936 (2016)
   PUBMED   27788492
  REMARK    GeneRIF: Enox1 in tumor stroma significantly enhances the
            effectiveness of 2 Gy fractionated radiotherapy and identifies
            Enox1 as a potential therapeutic target.
REFERENCE   5  (bases 1 to 3549)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 3549)
  AUTHORS   Kromkowski,J., Hignite,H., Morre,D.M. and Morre,D.J.
  TITLE     Response to lithium of a cell surface ECTO-NOX protein with
            time-keeping characteristics
  JOURNAL   Neurosci Lett 438 (1), 121-125 (2008)
   PUBMED   18462883
  REMARK    GeneRIF: Lithium at physiological concentrations induces an
            ECTO-NOX activity previously inactive from plant (soybean), murine
            (3T3 cells) and human (HUVEC and HeLa cells) sources and resets the
            period of the constitutive CNOX.
REFERENCE   7  (bases 1 to 3549)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3549)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK082388.1, AK051821.1 and BC052062.1.
            
            On Dec 2, 2011 this sequence version replaced NM_172813.2.
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            coding region, compared to variant 1. The resulting isoform (2)
            lacks an internal segment, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051821.1, SRR7345562.2820615.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AK082388.1         2-54
            54-3142             AK051821.1         2-3090
            3143-3549           BC052062.1         2711-3117
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 40.63 cM"
     gene            1..3549
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /note="ecto-NOX disulfide-thiol exchanger 1"
                     /db_xref="GeneID:239188"
                     /db_xref="MGI:MGI:2444896"
     exon            1..280
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            281..351
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            352..421
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            422..531
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            532..673
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            674..817
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             748..2679
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /note="isoform 2 is encoded by transcript variant 2; cNOX;
                     constitutive Ecto-NOX"
                     /codon_start=1
                     /product="ecto-NOX disulfide-thiol exchanger 1 isoform 2"
                     /protein_id="NP_766401.1"
                     /db_xref="CCDS:CCDS27290.1"
                     /db_xref="GeneID:239188"
                     /db_xref="MGI:MGI:2444896"
                     /translation="MVDAAGFESVAQCPRELHQMMAAAADGLGSIALDTTQLNMSVTD
                     PTAWATAMNNLGMVPVGLPGQQLVSDSICVPGFDPGLNMMTGITPINPMIPGLGLVPP
                     PPPTEVAVVKEIIHCKSCTLFPQNPNLPPPSTRERPPGCKTVFVGGLPENATEEIIQE
                     VFEQCGDITAIRKSKKNFCHIRFAEEFMVDKAIYLSGYRMRLGSSTDKKDSGRLHVDF
                     AQARDDFYEWECKQRMRAREERHRRKLEEDRLRPPSPPAIMHYSEHEAALLADKLKDD
                     SKFSEAITVLLSWIERGEVNRRSANQFYSMVQSANSHVRRLMNEKATHEQEMEEAKEN
                     FKNALTGILTQFEQIVAVFNASTRQKAWDHFSKAQRKNIDIWRKHSEELRNAQSEQLM
                     GIRREEEMEMSDDENCDSPTKKMRVDESALAAQAYALKEENDSLRWQLDAYRNEVELL
                     KQEKEQLFRTEENLTKDQQLQFLQQTMQGMQQQLLAIQEELNNKKSELEQAKEEQSHT
                     QALLKVLQEQLKGTKDLVETNGHSHEDANEINVLTVALVNQDRENNTEKRSQGLKSEK
                     EALLIGIISTFLHVHPFGANIEYLWSYMQQLDSKISANEIEMLLMRLPRMFKQEFTGV
                     GATLEKRWKLCAFEGIKTT"
     exon            818..955
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1129
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1336
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1570
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1783
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1890
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2008
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2193
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2194..2301
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2358
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2461
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2547
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..3498
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3476..3481
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3498
                     /gene="Enox1"
                     /gene_synonym="B230207J08; D230005D02Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcacgcccgc gcgccccgag ccgcggagtc tccagcgccc ctgagcagat cccactctcc
       61 cgggcgctgc tactcacaca ggcttccagc cgccacgggg cgtcctctgt ctgcggcagc
      121 agcggcggcg cgagcggagg ctgcagtcta gccgggtctg gctgctcggc gccctggaca
      181 gagtccggca gaagcgcgat cgggaggaga cgccgaggca aggaggagcc gcgccgcgcc
      241 cggcgctcgc ttgcctcgaa tccccgcacc gcggccccag gtccttgaca gccaacatga
      301 taaagtattg acaatacgcg gaggagagag agagagactc acatcgctca gactgtgagt
      361 cccagaagtt gatgaccttg tttggtgatt gggggtggaa ggaagaacag accccatgtg
      421 agaattaacg atttgcaaaa ccaaaagaag ccaaagcagg actgaaccgc acgagaggga
      481 tcaagacagg agtgaccagc cactgtaagt gagctggctc ccaagaggct gaagcatttg
      541 gcatgttgga gccatcctgt cttccgtggg acttcctttg aacagcttcc ataaaacact
      601 tctctgcata cattgtttca atcaagaccc gaaaggacag atatccgtct tcttctccac
      661 aggctgtttt aagagtccaa tgatctccga ggggaccctc tgctcagctg tcaaagcacc
      721 ccggcgcccc agggtgcgtg cagttcaatg gtagatgcgg ctggatttga gagcgttgcc
      781 cagtgtccac gggagcttca tcagatgatg gctgcagcag ctgatggttt ggggagcata
      841 gccctagata ccacccagct caacatgtcc gtcaccgatc ccacagcctg ggccacagct
      901 atgaataact tgggcatggt tcccgtgggg ttgcctggac agcagctcgt gtccgactca
      961 atctgcgtgc cgggcttcga tccaggcctc aacatgatga ctggaatcac ccccattaac
     1021 ccgatgatac ccggtctggg gctggtacca cccccacccc caacggaagt ggctgtcgtc
     1081 aaggaaataa tccactgcaa aagctgtact cttttccccc aaaatccaaa tcttccacct
     1141 ccttccacaa gagaacgacc tccagggtgt aagactgtgt ttgttggagg attaccagaa
     1201 aatgcaacgg aagaaattat tcaagaagtc tttgagcagt gtggagatat cacagccatc
     1261 cgaaagagca agaagaattt ctgtcacatt cgctttgccg aggaattcat ggttgacaaa
     1321 gccatttacc tctctggtta cagaatgcga ttagggtcga gcacggataa aaaggattcc
     1381 ggccgcctgc acgtggattt tgctcaggcc agggatgact tctatgagtg ggaatgcaag
     1441 caaaggatgc gcgcccggga ggagcggcat cgacgcaaac tggaagagga caggctgcgg
     1501 cctccctcgc cgccggccat catgcactac tcggagcacg aagctgccct gctggctgac
     1561 aaactgaaag atgacagcaa gttctctgag gctatcacag ttctgctgtc ctggatcgag
     1621 cgtggggagg tgaaccggcg ctcggcaaac cagttctact ccatggttca gtcggccaac
     1681 agccacgtgc gcaggctcat gaatgaaaag gctacccacg agcaggaaat ggaggaagca
     1741 aaggagaact tcaaaaatgc cttaacgggg atcctcactc aatttgagca gattgtggcc
     1801 gttttcaacg cttctaccag acaaaaagct tgggaccatt tctcgaaagc ccagcgcaag
     1861 aacatagaca tttggcgaaa gcactctgag gagctccgga acgctcaaag cgagcagctc
     1921 atgggcatcc gccgtgagga ggagatggaa atgtctgatg atgagaactg tgacagcccg
     1981 actaaaaaaa tgagagtcga tgaatcagcc ctggctgctc aggcctacgc cctcaaagag
     2041 gagaatgaca gtctccggtg gcaactggat gcctacagga acgaggtgga gctgctgaag
     2101 caagagaagg aacagctctt ccggacagaa gaaaacctca ccaaggacca acagctacag
     2161 ttcctgcagc aaaccatgca aggcatgcag cagcaattgc tggccatcca ggaggagctg
     2221 aacaacaaaa agtcggaatt ggagcaggca aaggaggagc agtctcacac gcaagcgttg
     2281 ctgaaagtcc tccaggagca gttaaaaggt accaaggact tggtggagac gaatggccac
     2341 agccacgagg atgcaaatga aatcaatgtg ttgacggtcg cgttggttaa ccaggaccga
     2401 gagaacaaca ctgaaaagag aagccaaggt ttgaaatctg agaaggaggc cctgctgata
     2461 ggcatcatat caacgtttct tcacgttcac ccctttggag ctaatataga gtatctgtgg
     2521 tcatacatgc agcagctgga ctctaagata tccgccaatg aaattgaaat gcttctgatg
     2581 aggctgccac ggatgttcaa acaggaattc actggtgtgg gagcgacgct agagaagaga
     2641 tggaagttgt gtgcctttga aggaattaaa acgacctaac tgacgtccag gagagctgga
     2701 agcgaaaccg tctgaagcca gccagggctg tacagtacag gagcaggagg gctggggtct
     2761 gccagcgtgg gacctgtgcg gagccaccaa agctttgttc agtgacatct gtggcgtttt
     2821 catgtgcgtg gaaatgtaat tgtgtaccag ttccttacac tagaccaagc ctcacactat
     2881 gccagacctg tccctatgaa taccaagcaa agattccaca gtcaatgatc acaaaatctc
     2941 aaactaggcc ttgctgcaga atagagcaaa tgcttacagt taatggcgct gtagcaatgg
     3001 tggttgccag gctacagtta tgtatgtaat gtatagctga caacattaaa tgacattttc
     3061 ctgatggtct ttctgttttc gttaataaaa caacaacaaa acaacaaaaa agtgatttta
     3121 cagtggggga aaaaatcata ccaaaagaaa acttgaatat gtgtttgaac agttattgta
     3181 ttttgtaaat taagttatat gctgttccag ggacttttgc cttattgaag aggcagaaaa
     3241 ctatgattgg actccttgag aatgctttat caaggatatt atttttctaa tgtaacggtt
     3301 ctccatcgcg tattcttttc acatggactg catagcaatt ttcataaaat gtaaatagaa
     3361 ggaacaactg ctgctgtccc gtgcttggta tgtaacattc aggtttatgc attgaaaaaa
     3421 acattcggaa aatattcctg ttacaaattt tatagcaaag atattaattt ttttcaataa
     3481 atcttgactt ctaccataaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3541 aaaaaaaaa
//
