LOCUS       NM_178689               2592 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus enolase 4 (Eno4), mRNA.
ACCESSION   NM_178689 XM_985284
VERSION     NM_178689.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Nakamura,N., Dai,Q., Williams,J., Goulding,E.H., Willis,W.D.,
            Brown,P.R. and Eddy,E.M.
  TITLE     Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4)
            gene causes sperm structural defects and male infertility
  JOURNAL   Biol Reprod 88 (4), 90 (2013)
   PUBMED   23446454
  REMARK    GeneRIF: ENO4 is required for normal assembly of the fibrous sheath
            and provides most of the enolase activity in sperm.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
REFERENCE   3  (bases 1 to 2592)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102604.13.
            
            On Apr 26, 2022 this sequence version replaced NM_178689.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.112675.1,
                                           SRR17784645.1206169.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-222               AC102604.13        101667-101888       c
            223-351             AC102604.13        99626-99754         c
            352-542             AC102604.13        98446-98636         c
            543-697             AC102604.13        91997-92151         c
            698-867             AC102604.13        89976-90145         c
            868-999             AC102604.13        89739-89870         c
            1000-1053           AC102604.13        87648-87701         c
            1054-1110           AC102604.13        86226-86282         c
            1111-1278           AC102604.13        84998-85165         c
            1279-1404           AC102604.13        82381-82506         c
            1405-1600           AC102604.13        80715-80910         c
            1601-1668           AC102604.13        76719-76786         c
            1669-1786           AC102604.13        76098-76215         c
            1787-2592           AC102604.13        73867-74672         c
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 54.55 cM"
     gene            1..2592
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /note="enolase 4"
                     /db_xref="GeneID:226265"
                     /db_xref="MGI:MGI:2441717"
     exon            1..222
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..1914
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /EC_number="4.2.1.11"
                     /note="enolase-like protein C10orf134 homolog;
                     enolase-like protein ENO4; 2-phospho-D-glycerate
                     hydro-lyase"
                     /codon_start=1
                     /product="enolase 4"
                     /protein_id="NP_848804.2"
                     /db_xref="CCDS:CCDS79725.1"
                     /db_xref="GeneID:226265"
                     /db_xref="MGI:MGI:2441717"
                     /translation="MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTF
                     YLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICA
                     VAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHR
                     LRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDT
                     LLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTF
                     SMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLP
                     PPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVN
                     FHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKED
                     AEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVE
                     ITHLINGKKLLAVFGSTDSESSDDSLVDLAVGFGARFIKLGGLSRGERMTKYNRLLAI
                     EEELIQRGVWGFSEEHNFSFFQEDATATMAEETLGLLDSIFPTEVIEESAKT"
     exon            223..351
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            352..542
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            543..697
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            698..867
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            868..999
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1053
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1110
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1278
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1404
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1600
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1668
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1786
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..2592
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2078..2083
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /note="hexamer: AATATA"
     polyA_site      2104
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /note="major polyA site"
     regulatory      2571..2576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2592
                     /gene="Eno4"
                     /gene_synonym="6430537H07Rik"
ORIGIN      
        1 aaaaggacgc tggcgggccc cctggcaaaa ccccgctgta gccctaagtc tcctaccatg
       61 ggagatgaag acggcggccg cagaggcggg atcactaggg acctgcagaa gctgaagcag
      121 caggcgatgg cgtactacca ggagaacgac gtcccgcgca agctggaaga tctgctcaac
      181 tccaccttct acctccagcc tgcggatgtc tacgggcacc tggcaaacta cttttctaaa
      241 cttgcgaagc ctccctccat atgcaaaata gtagggaaaa ccatactgga tggcctggga
      301 cttcctactc tacaggtgga aatctcctgc accattcaaa atttccccaa gtacatctgt
      361 gccgtggcga tccctacgca ctttgaagtc gtggagaacg ctttgccaga ggcgttggat
      421 gcagaggact cagaaagggc ccaggccgtg aacacagcgg tgcagtggat caatcagtcc
      481 atcacagagg agctgtgggg tctggtcccc tccaaccagg cagaggtgga ccacaggctc
      541 aggaccttct ttgaacataa agtacaagaa gataaagaga gaaaagaatt ggaaaagagc
      601 caggaggagc tggttcccgc acctccacca gtaacgctgc cgccgccgcc gccgccgcct
      661 cccccacctc cctccaaaaa gaaagggcag aaggcaggac ggagggatac gcttttggag
      721 aaacctgttt cacccccgga gccccctgaa ccagtcctcc atggtagcat ggccataggc
      781 gctgtgtccc tggcagtggc caaagccagc gcaaccttgg ccagcgatcc tctctactta
      841 accctggcgt cactgaagca tgatcaggag cagccgtcaa cattctctat gcctttgctg
      901 atgggatctg tactgagctg tgggaagtcg tcacctggga agctacattt aatgaaagaa
      961 gtgatttgta ttcccagtcc tggattaaca gccaaacaaa gtgtcgagtt gcttctggaa
     1021 attcagaaac aagttaacag agcgatggag acgcttccac ctccaaaaca agagaccaaa
     1081 aaagggcata atgggagcaa aagagctcag ccaccgatca ctggcaaggt atctcacctt
     1141 ggctgtttaa ccatcaacta cgacgccata gagcagccac tgctgctgct tcaggggatc
     1201 tgctcaaacc tggggttgga actgggagtg aacttccatc tagccatcaa ctgtgctggg
     1261 catgagctga tggactacag taaagggaag tacgaagtga tggtgggcac ccacaaaagc
     1321 gcagctgaga tggtggagct ctacgtggac ctgatcaaca agtatccttc cataatcgcc
     1381 ttaattgatc ctttcaggaa ggaggacgcc gagcagtggg acagcctcta tgctgctctg
     1441 gcttcccggt gctacctaat tgcaggcgcc gcttccggga gtgtctctaa actcctagag
     1501 tgcagaaata taagcaccct gaaatcccac ggactgatca taaagcacac aaaccaaacc
     1561 acaatgtctg acttggtgga aataacccat cttatcaacg gtaagaagct cctggccgtc
     1621 tttggaagca cagactcgga gtcctctgat gacagccttg tcgatttggc tgttggattc
     1681 ggtgcccggt ttatcaagtt ggggggtctt tctcggggtg aacggatgac caaatacaac
     1741 cgccttcttg ctatagagga agaactcatc cagaggggag tatggggttt cagtgaagaa
     1801 cacaattttt ctttctttca agaggatgct actgccacaa tggctgagga aactcttggg
     1861 ctcctggact ccatcttccc cacagaggtg atagaggaat cggctaaaac atgagcctcc
     1921 ctcccggttc tggactccca gggcacagcc actccaccag tgtgggccag ctggtgtgaa
     1981 gtgcctccac ggtgtggctc cgctctgaac tccacttata tccgtaaatg agtataatca
     2041 ccaacgtgtg catgctttcg gtttcaccgt cgggtaaaat atatgcctgg tgggtttgcc
     2101 tgcagctcat gagctttact ttcgaggcca ctttgccatg ggacactagc tccactgtct
     2161 ccttctgaaa gtcatgacct aagttcatta gaggctaatg tatttccgat gtcgggttca
     2221 cgcccagtca cgcacccctc ataaatcaga gcggctctag caccaaaaga ctgtttccct
     2281 gagtagctcc cggcaccaca caggaatcct ctgattttgt ttctcttgga cctctgtgct
     2341 tggaccacag atgctctgcc tagccaccaa catgcaccaa gccttgtctg cagagatact
     2401 tccatcttag cttccaacat tgattacctg tgcctgtatt gatacttcta tcttagcttc
     2461 tgacacttga ttacctgtgt ctgtgccttt cctcatccct ctggtgtgtt catcatccct
     2521 gtattatttt ttcccttagg tcccttttga tacatgagtg ggattaactt aataaaaaca
     2581 cttgtaaatg aa
//
