LOCUS       NM_027728               1227 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus enkurin, TRPC channel interacting protein (Enkur),
            mRNA.
ACCESSION   NM_027728 XM_001000176 XM_283704 XM_911600
VERSION     NM_027728.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1227)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   4  (bases 1 to 1227)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   5  (bases 1 to 1227)
  AUTHORS   Jungnickel,M.K., Sutton,K.A., Baker,M.A., Cohen,M.G.,
            Sanderson,M.J. and Florman,H.M.
  TITLE     The flagellar protein Enkurin is required for mouse sperm motility
            and for transport through the female reproductive tract
  JOURNAL   Biol Reprod 99 (4), 789-797 (2018)
   PUBMED   29733335
  REMARK    GeneRIF: Sperm flagella of mice homozygous for a mutated allele
            displayed only asymmetric bending, nonprogressive motility, and a
            loss of Ca2+-responsiveness following demembranation. We propose
            that Enkurin is part of a flagellar Ca2+-sensor that regulates
            bending and that the motility defects following mutation of the
            locus are the proximate cause of subfertility.
REFERENCE   6  (bases 1 to 1227)
  AUTHORS   Sigg,M.A., Menchen,T., Lee,C., Johnson,J., Jungnickel,M.K.,
            Choksi,S.P., Garcia,G. 3rd, Busengdal,H., Dougherty,G.W.,
            Pennekamp,P., Werner,C., Rentzsch,F., Florman,H.M., Krogan,N.,
            Wallingford,J.B., Omran,H. and Reiter,J.F.
  TITLE     Evolutionary Proteomics Uncovers Ancient Associations of Cilia with
            Signaling Pathways
  JOURNAL   Dev Cell 43 (6), 744-762 (2017)
   PUBMED   29257953
REFERENCE   7  (bases 1 to 1227)
  AUTHORS   McClintock,T.S., Glasser,C.E., Bose,S.C. and Bergman,D.A.
  TITLE     Tissue expression patterns identify mouse cilia genes
  JOURNAL   Physiol Genomics 32 (2), 198-206 (2008)
   PUBMED   17971504
  REMARK    GeneRIF: 4933434I06Rik is most abundantly expressed in tissues rich
            in highly ciliated cells, such as olfactory sensory neurons, and is
            predicted to be important to cilia.
REFERENCE   8  (bases 1 to 1227)
  AUTHORS   Sutton,K.A., Jungnickel,M.K., Wang,Y., Cullen,K., Lambert,S. and
            Florman,H.M.
  TITLE     Enkurin is a novel calmodulin and TRPC channel binding protein in
            sperm
  JOURNAL   Dev Biol 274 (2), 426-435 (2004)
   PUBMED   15385169
  REMARK    GeneRIF: Data suggest that enkurin is an adaptor that functions to
            localize a Ca2+ sensitive signal transduction machinery in sperm to
            a Ca2+-permeable ion channel [enkurin]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK017056.1 and BC099519.1.
            
            On or before Feb 3, 2007 this sequence version replaced
            XM_283704.3, XM_911600.2, XM_001000176.1, NM_027728.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017056.1, AY454124.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1126              AK017056.1         2-1127
            1127-1227           BC099519.1         1026-1126
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 14.82 cM"
     gene            1..1227
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /note="enkurin, TRPC channel interacting protein"
                     /db_xref="GeneID:71233"
                     /db_xref="MGI:MGI:1918483"
     exon            1..294
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..985
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /codon_start=1
                     /product="enkurin"
                     /protein_id="NP_082004.1"
                     /db_xref="CCDS:CCDS38055.1"
                     /db_xref="GeneID:71233"
                     /db_xref="MGI:MGI:1918483"
                     /translation="MDSPCTSESIYNLIPSDLKEPPQHPRYTSLFRATIKNDMKKFKT
                     AMKTMGPAKVEIPSPKDFLKKHSKEKTLPPKKKFNRCSPKKPAVPLRTDHPVMGIQSG
                     KNFINTNAADVIMGVAKKPKPIYVDKRTGDKHDLETSGLFPKYINKKDYGITPEYICK
                     RNEDVKKAQEEYDNYIQENLKKAAMKRLSDEEREAVLQGLKKNWEEVHKEFQSLSVFI
                     DSVPKKIRKQKLEKEMKQLEHDISVIEKHKIIYIANK"
     exon            295..440
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            441..664
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            665..811
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            812..1199
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1164..1169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /note="hexamer: TATAAA"
     regulatory      1176..1181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1185
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
     polyA_site      1194
                     /gene="Enkur"
                     /gene_synonym="4933434I06Rik"
                     /note="major polyA site"
ORIGIN      
        1 accacgtggt caacaacgct aaacagactc ggagcagaat tattttcttc ctgaaaaagg
       61 gactgttacg aaagggtcaa agggaaatta cacgaaagag aaattttagt tctttgataa
      121 gaaagtaaga aaagaaaaag gcggtgagag aaggagttga gacagtggga aagtgagcca
      181 aaaagctgag tacttgttaa ggaattcctt ggtggccatg gactcaccct gcacttccga
      241 gagtatttat aacctcatac ccagtgactt gaaggagccg ccccagcatc ctaggtatac
      301 atcactgttt agagcaacta taaaaaatga catgaagaaa tttaaaacgg caatgaaaac
      361 catgggacct gcaaaagtag agataccttc ccccaaggat tttctaaaga agcattccaa
      421 ggaaaaaaca ctaccaccaa aaaaaaagtt taataggtgc tctcccaaga agcctgcagt
      481 gcccttgaga accgatcatc cagttatggg aatacagagt ggaaaaaact tcataaacac
      541 aaatgcagct gacgtcatca tgggcgtggc caaaaagccc aagccgattt atgttgacaa
      601 aagaactgga gataagcatg accttgaaac ttcagggcta ttccccaagt acatcaacaa
      661 aaaggattat ggcatcacgc ctgagtacat atgcaagcga aatgaggatg tgaagaaagc
      721 acaagaagag tatgacaatt acatccagga gaacctcaag aaagcggcca tgaagagact
      781 ctctgacgaa gaaagggagg cagttctgca gggactgaag aagaactggg aagaggtgca
      841 caaagagttc caatccctct cggtcttcat tgattctgta ccaaagaaga ttcgcaagca
      901 gaagctggaa aaagagatga agcagctgga acacgacatc agtgtcattg agaagcacaa
      961 gatcatctac atcgctaaca agtgagccaa ctgttgcagg acagaaaaaa gccacatggc
     1021 catcacacta aacccactct tctcaaagag gactatgaag agaataaagt tttcaccgaa
     1081 aatgtagggg cagtgttgaa agaatagttg aattatttgc ttgctctaga gaaaattttc
     1141 tcctccactg tcagagttct acttataaac aaaccattaa agtcagaagc tgcaccttga
     1201 aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
