LOCUS       NM_172573               4083 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus endo-beta-N-acetylglucosaminidase (Engase), transcript
            variant 1, mRNA.
ACCESSION   NM_172573
VERSION     NM_172573.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Yoshida,Y., Asahina,M., Murakami,A., Kawawaki,J., Yoshida,M.,
            Fujinawa,R., Iwai,K., Tozawa,R., Matsuda,N., Tanaka,K. and
            Suzuki,T.
  TITLE     Loss of peptide:N-glycanase causes proteasome dysfunction mediated
            by a sugar-recognizing ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 118 (27) (2021)
   PUBMED   34215698
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Maynard,J.C., Fujihira,H., Dolgonos,G.E., Suzuki,T. and
            Burlingame,A.L.
  TITLE     Cytosolic N-GlcNAc proteins are formed by the action of
            endo-beta-N-acetylglucosaminidase
  JOURNAL   Biochem Biophys Res Commun 530 (4), 719-724 (2020)
   PUBMED   32782141
  REMARK    GeneRIF: Cytosolic N-GlcNAc proteins are formed by the action of
            endo-beta-N-acetylglucosaminidase.
REFERENCE   3  (bases 1 to 4083)
  AUTHORS   Fujihira,H., Masahara-Negishi,Y., Akimoto,Y., Hirayama,H.,
            Lee,H.C., Story,B.A., Mueller,W.F., Jakob,P., Clauder-Munster,S.,
            Steinmetz,L.M., Radhakrishnan,S.K., Kawakami,H., Kamada,Y.,
            Miyoshi,E., Yokomizo,T. and Suzuki,T.
  TITLE     Liver-specific deletion of Ngly1 causes abnormal nuclear morphology
            and lipid metabolism under food stress
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (3), 165588 (2020)
   PUBMED   31733337
REFERENCE   4  (bases 1 to 4083)
  AUTHORS   Fujihira,H., Masahara-Negishi,Y., Tamura,M., Huang,C., Harada,Y.,
            Wakana,S., Takakura,D., Kawasaki,N., Taniguchi,N., Kondoh,G.,
            Yamashita,T., Funakoshi,Y. and Suzuki,T.
  TITLE     Lethality of mice bearing a knockout of the Ngly1-gene is partially
            rescued by the additional deletion of the Engase gene
  JOURNAL   PLoS Genet 13 (4), e1006696 (2017)
   PUBMED   28426790
  REMARK    GeneRIF: These observations strongly suggest that the Ngly1- or
            Ngly1/Engase-deficient mice could serve as a valuable animal model
            for studies related to the pathogenesis of the NGLY1-deficiency,
            and that cytoplasmic ENGase represents one of the potential
            therapeutic targets for this genetic disorder.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4083)
  AUTHORS   Harada,Y., Huang,C., Yamaki,S., Dohmae,N. and Suzuki,T.
  TITLE     Non-lysosomal Degradation of Singly Phosphorylated Oligosaccharides
            Initiated by the Action of a Cytosolic
            Endo-beta-N-acetylglucosaminidase
  JOURNAL   J Biol Chem 291 (15), 8048-8058 (2016)
   PUBMED   26858256
  REMARK    GeneRIF: ENGase efficiently hydrolyzes phosphorylated
            oligosaccharides that are larger than Man4GlcNAc2-P, generating
            GlcNAc-1-P and neutral Gn1-type free oligosaccharides.
REFERENCE   6  (bases 1 to 4083)
  AUTHORS   Huang,C., Harada,Y., Hosomi,A., Masahara-Negishi,Y., Seino,J.,
            Fujihira,H., Funakoshi,Y., Suzuki,T., Dohmae,N. and Suzuki,T.
  TITLE     Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates
            during ER-associated degradation in Ngly1-defective cells
  JOURNAL   Proc Natl Acad Sci U S A 112 (5), 1398-1403 (2015)
   PUBMED   25605922
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY070749.1, AK048667.1 and BC060640.1.
            
            On Jan 3, 2018 this sequence version replaced NM_172573.2.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               BY070749.1         4-131
            129-4050            AK048667.1         3-3924
            4051-4083           BC060640.1         4293-4325
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 83.21 cM"
     gene            1..4083
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /note="endo-beta-N-acetylglucosaminidase"
                     /db_xref="GeneID:217364"
                     /db_xref="MGI:MGI:2443788"
     exon            1..273
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..2359
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /EC_number="3.2.1.96"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cytosolic endo-beta-N-acetylglucosaminidase"
                     /codon_start=1
                     /product="cytosolic endo-beta-N-acetylglucosaminidase
                     isoform 1"
                     /protein_id="NP_766161.1"
                     /db_xref="CCDS:CCDS25704.1"
                     /db_xref="GeneID:217364"
                     /db_xref="MGI:MGI:2443788"
                     /translation="METSSVLTRGAARQRSPAAPEKQARDQTERRPGRRRQGRRINED
                     QEEEAVFREVVSFTPDPLPARYYDKDTTRPISFYLSTLEELLAWTPLMEDGFNVALEP
                     LVCRRPPLSSPRPRTLLCHDMMGGYLEDRFIQGSEVQNPYSFYHWQYIDIFVYFSHHT
                     VTIPPVCWTNAAHRHGVCVLGTFITEWQEGGRLCEAFLAGDEPSFQAVADRLVQIAQF
                     FRFDGWLINIENSLTPAAVRNTPLFLQYLTAQLHQQVPGGLVLWYDSVVQSGQLKWQD
                     ELNDQNRVFFDSCDGFFTNYNWREDHLQRMVAQAGERLADVYVGVDVFARSNVVGGRF
                     DTDKSLELIRKHGFSAALFAPGWVYECLEKSDFFQNQDKFWSLLERFLPTHSICSLPF
                     VTSFCLGLGTRRVCYGKEQAVGPWYHPSAQETQPLFGEHKLAGDSRGWVKTHCCLTDA
                     WHGGSSLLLRGLIPPEVDSVAVRLFSLHIPVPPKVFLSMVYKFEGSTDVQVALELTTG
                     DASSCHVGGMLVLNETGSRHSPRPLRVPPTRLARWASSCGQQLSGGWIQRCYEVNLRG
                     CLLQDLLVSFSRPPGSREEESFICRLGEIQVVDASSLLAPLPRVQNVTISQIRWLPLI
                     TGSEGLPTRLLLSCTLHWSYLLLRARCFRIHCWKRTGSSSSVAESPETEKPTFLGLAF
                     ANQYRVVDLAVEAAGFGQDGRVEFLVEPVPREGFLVPQAEWGKAVLLFSVPQ"
     exon            274..344
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            345..546
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            547..695
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            696..853
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            854..1002
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1168
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1272
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1381
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1572
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1721
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1827
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1942
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..4061
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2608..2613
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /note="hexamer: CATAAA"
     polyA_site      2636
                     /gene="Engase"
                     /gene_synonym="C130099A03; D230014K01Rik"
                     /note="major polyA site"
ORIGIN      
        1 acgtggacag atcctagccg gaactcggtg cgagccaacc gcggcgctgc acactcacca
       61 ttggctgaga gctgcggcag gggcaggccc aggcttgcga ttggctgttg gagcagtgtc
      121 tggagggctt agcggtcggc agtcgctgac agtcatggag acctcgtcgg tgctgacccg
      181 gggagctgca aggcagcgga gccccgcggc cccggagaag caggcgcggg accagacgga
      241 gcggaggccg gggcggcgga ggcaggggcg gaggatcaac gaggatcaag aggaggaagc
      301 agtctttaga gaggtggtca gtttcacccc ggatccttta ccagctagat attatgacaa
      361 ggatactacc agacctatca gcttttacct gtcgacgctg gaggagctcc tggcctggac
      421 cccgctcatg gaggatggct tcaatgtagc cctggaaccc ctggtgtgtc gccggcctcc
      481 actcagcagc cccaggcccc ggaccctgct gtgccacgac atgatgggtg gttacctgga
      541 ggacaggttc attcagggct cagaggtgca gaacccctat tccttctacc actggcagta
      601 tatcgacatc tttgtgtact tcagccacca cacggtcacc atccccccag tgtgctggac
      661 caatgccgca cacaggcacg gggtctgcgt gctggggacc ttcatcacag agtggcaaga
      721 gggaggcagg ctctgcgaag ccttcctggc cggggatgag ccctccttcc aggccgtggc
      781 cgatcggctg gtccagattg ctcagttctt tcgttttgac ggctggctca tcaacatcga
      841 gaactccctg actccagctg ccgtgaggaa cacaccttta tttctccagt accttaccgc
      901 acagctccat cagcaggtcc ccggaggtct ggtgctgtgg tacgacagtg tggtacagag
      961 cgggcagctc aagtggcagg atgaactgaa tgatcagaac cgggtcttct ttgactcctg
     1021 cgatggcttc tttaccaact ataactggcg ggaggatcac ctgcagcgga tggtggcaca
     1081 ggccggggag cgcctggctg acgtgtacgt gggagtggat gtgtttgctc ggagcaatgt
     1141 ggtcggaggc cgttttgata ctgacaagtc cctggagctg atccgcaaac atggcttctc
     1201 ggcggcgctg tttgctcctg ggtgggtgta tgagtgtctg gagaagagcg acttctttca
     1261 gaaccaggac aagttctgga gcctgctgga gcgcttcctt cccacacaca gtatctgctc
     1321 cctgcccttc gtcacctctt tctgcctggg cctgggtact cgaagagtct gctacggcaa
     1381 ggagcaagca gtggggccct ggtaccaccc tagcgcccag gagacacagc ccctctttgg
     1441 tgaacataag ctggctggtg acagtcgggg ctgggtgaag acgcactgct gcctgactga
     1501 tgcctggcac gggggcagct ctctgctgct ccggggcctg atcccgcctg aggttgacag
     1561 tgtagctgtg aggctgttct ctctgcacat cccagtgcca cccaaggttt tcctgtccat
     1621 ggtgtacaag tttgaaggct caacagatgt ccaggtggct ttggagctca caacagggga
     1681 tgccagcagc tgtcatgtgg gtggcatgtt ggtgttaaat gaaactggct cacggcacag
     1741 ccccagaccc ctccgggtac cccccaccag gctggccaga tgggccagca gttgtggcca
     1801 gcagctcagt gggggctgga ttcaacgctg ctatgaggta aaccttcgtg ggtgcctgct
     1861 ccaggacctc ttggtgagct tctcacggcc gccaggcagc cgggaggagg agagcttcat
     1921 ttgtcgcctt ggagagatcc aggtggttga tgccagcagc cttctggccc ctctgccccg
     1981 ggtgcagaat gtcaccattt ctcagatacg atggcttcct ctgatcaccg ggtctgaggg
     2041 cctccctacc cggctcctcc tcagctgcac cctgcactgg tcctacctgc tgctccgagc
     2101 ccgctgcttc cgcattcact gctggaaacg gacaggaagc agcagctctg tggcagagtc
     2161 cccagagaca gagaagccca cgttcctggg cctggctttc gccaaccagt accgtgtggt
     2221 ggacttggct gtggaggctg ctggatttgg gcaggatggt cgtgtggagt tcctggtgga
     2281 gcctgtcccc agggagggtt tcctggtgcc ccaggctgag tggggaaagg cggtcctgct
     2341 cttctcggtc cctcagtgag cccgctccag agcacagcgt ctcctcgccc acctcaagac
     2401 tcgaggtttc ctcttggcca gccgcccaag gctgcgtgga ctgccgagga cagccggatc
     2461 cccaggagat gaggtctcca ggttctggct gtgactcagg aagctgtgcc ccacgggtgc
     2521 taggcgagtt ccccttccac agtgctctcc gtgggatgtg gttctcaagg aaggaggaga
     2581 aagtttgagg tttcttccgg aaaattccat aaaggctgtt tctccgaggc tgaaaaggtg
     2641 cttcttttga gaatggcggc catttatcct gtagctttga caatcagagg caaacggcac
     2701 gggtggcagg cagtaaggat ccctgaagac tgagaggata gtcaggggag gagtgggagt
     2761 cagggttgct cacggagtag ctggagtggt gctccccagg accaggagca ccatcaggtg
     2821 gctcctggca ccacaccccc acctgtataa ggtgggccag tcacatgtca cacttcagac
     2881 taactgtatg gatggtggct ctggcagctg aatgtcccag agaagcccaa attcctcacc
     2941 tgtgtgatat gagggtgcta accaccatct catgtccctc aagaggcagg tgagccccgc
     3001 ctgcttccag gtacagtggc atccttagct gtaccgaagc cctgttgaca ctaggctgcg
     3061 tgggaacttc ctgctgtgtg actgcaacct gggtgatgac cccaccactt tccccccgcc
     3121 tctcccagtg cttcgtgctc agggcacagt ggaactctca tcctttctgt gttccaaatg
     3181 tgaccctctt tgacacagct ctgtggtctg acctgtggcc ctcatgctgt agtatgggga
     3241 gaggccgcaa gatggcagag cagcctgttg ccatcttcca tggtgtaggg actgaatgga
     3301 tggggtttcc tcagattcct gagtgtgggg caaggagaga ggctcctgcc tgcatgggct
     3361 gagcgctgcc ctgtcacccc catcttcctt aatttcctgg gcaccctgtt cctcttgagt
     3421 gaagtataag ggtgaggacc tagaacctga gattgggaaa ttctgcactg ttccatgtgg
     3481 ttctaccagc tctcacatag acagggtgca gggcagggcc tctggtaaag cccaggctac
     3541 tactgtactc tggcatggct ctgccaacct tgtacgggat tggggtaggg agagtacctg
     3601 gagctgtgct ggcctccctc tggtttgtcg tccagaatct gccccgggag ccttggctca
     3661 cccatgccca gccagggttc tgtgtgactg gagtggaatc tgtggggagc agagtggctg
     3721 gctggctctg ggcagggcag caatttctga atgccctgct tccccgtggt acagagtatg
     3781 cttttaaaag tgctggttcc ttcaaatatt ttatgcagag aaggagagaa aatttatagt
     3841 ggcaattatt ccctcacagt ctggtgagca agcaattaga aggaaggggg cttagcggag
     3901 ccacggcgtg aggtagagca gcgcaacctc acttcccagg agcacggccc ccagtcccgg
     3961 caacgctggc tcctgcgcag gaaaattaat ctgccggcat ttaagcctag gcccctctgg
     4021 tggatataat cgctcagaaa taaattatcc aatgtgaaaa aaaaaaaaaa aaaaaaaaaa
     4081 aaa
//
