LOCUS       NM_010134               3576 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus engrailed 2 (En2), transcript variant 1, mRNA.
ACCESSION   NM_010134
VERSION     NM_010134.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Trinh,L.T., Finnel,R.R., Osipovich,A.B., Musselman,J.R.,
            Sampson,L.L., Wright,C.V.E. and Magnuson,M.A.
  TITLE     Positive autoregulation of Sox17 is necessary for gallbladder and
            extrahepatic bile duct formation
  JOURNAL   Development 152 (2) (2025)
   PUBMED   39745200
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Mattioni,L., Barbieri,A., Grigoli,A., Balasco,L., Bozzi,Y. and
            Provenzano,G.
  TITLE     Alterations of Perineuronal Net Expression and Abnormal Social
            Behavior and Whisker-dependent Texture Discrimination in Mice
            Lacking the Autism Candidate Gene Engrailed 2
  JOURNAL   Neuroscience 546, 63-74 (2024)
   PUBMED   38537894
  REMARK    GeneRIF: Alterations of Perineuronal Net Expression and Abnormal
            Social Behavior and Whisker-dependent Texture Discrimination in
            Mice Lacking the Autism Candidate Gene Engrailed 2.
REFERENCE   3  (bases 1 to 3576)
  AUTHORS   Lazzarini,G., Gatta,A., Miragliotta,V., Vaglini,F., Viaggi,C. and
            Pirone,A.
  TITLE     Glial cells are affected more than interneurons by the loss of
            Engrailed 2 gene in the mouse cerebellum
  JOURNAL   J Anat 244 (4), 667-675 (2024)
   PUBMED   38009365
  REMARK    GeneRIF: Glial cells are affected more than interneurons by the
            loss of Engrailed 2 gene in the mouse cerebellum.
REFERENCE   4  (bases 1 to 3576)
  AUTHORS   Song,X., Hu,Q., Xu,X. and Pan,W.
  TITLE     Protein kinase C beta relieves autism-like behavior in EN2 knockout
            mice via upregulation of the FTO/PGC-1alpha/UCP1 axis
  JOURNAL   J Biochem Mol Toxicol 37 (1), e23236 (2023)
   PUBMED   36239013
  REMARK    GeneRIF: Protein kinase C beta relieves autism-like behavior in EN2
            knockout mice via upregulation of the FTO/PGC-1alpha/UCP1 axis.
REFERENCE   5  (bases 1 to 3576)
  AUTHORS   Wang,Y., Herzig,G., Molano,C. and Liu,A.
  TITLE     Differential expression of the Tmem132 family genes in the
            developing mouse nervous system
  JOURNAL   Gene Expr Patterns 45, 119257 (2022)
   PUBMED   35690356
REFERENCE   6  (bases 1 to 3576)
  AUTHORS   Shimamura,K. and Takeichi,M.
  TITLE     Local and transient expression of E-cadherin involved in mouse
            embryonic brain morphogenesis
  JOURNAL   Development 116 (4), 1011-1019 (1992)
   PUBMED   1295725
REFERENCE   7  (bases 1 to 3576)
  AUTHORS   Conlon,R.A. and Rossant,J.
  TITLE     Exogenous retinoic acid rapidly induces anterior ectopic expression
            of murine Hox-2 genes in vivo
  JOURNAL   Development 116 (2), 357-368 (1992)
   PUBMED   1363087
REFERENCE   8  (bases 1 to 3576)
  AUTHORS   Bally-Cuif,L., Alvarado-Mallart,R.M., Darnell,D.K. and Wassef,M.
  TITLE     Relationship between Wnt-1 and En-2 expression domains during early
            development of normal and ectopic met-mesencephalon
  JOURNAL   Development 115 (4), 999-1009 (1992)
   PUBMED   1360404
REFERENCE   9  (bases 1 to 3576)
  AUTHORS   Yamaguchi,T.P., Conlon,R.A. and Rossant,J.
  TITLE     Expression of the fibroblast growth factor receptor FGFR-1/flg
            during gastrulation and segmentation in the mouse embryo
  JOURNAL   Dev Biol 152 (1), 75-88 (1992)
   PUBMED   1321062
REFERENCE   10 (bases 1 to 3576)
  AUTHORS   Wilkie,T.M., Gilbert,D.J., Olsen,A.S., Chen,X.N., Amatruda,T.T.,
            Korenberg,J.R., Trask,B.J., de Jong,P., Reed,R.R., Simon,M.I. et
            al.
  TITLE     Evolution of the mammalian G protein alpha subunit multigene family
  JOURNAL   Nat Genet 1 (2), 85-91 (1992)
   PUBMED   1302014
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK157766.1 and AI844870.1.
            
            On May 9, 2018 this sequence version replaced NM_010134.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK157766.1, L12705.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3537              AK157766.1         3-3539
            3538-3576           AI844870.1         1-39                c
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 13.94 cM"
     gene            1..3576
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /note="engrailed 2"
                     /db_xref="GeneID:13799"
                     /db_xref="MGI:MGI:95390"
     exon            1..1489
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             832..1806
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     homeobox protein engrailed-2; homeobox protein en-2"
                     /codon_start=1
                     /product="homeobox protein engrailed-2 isoform 1"
                     /protein_id="NP_034264.1"
                     /db_xref="CCDS:CCDS19143.1"
                     /db_xref="GeneID:13799"
                     /db_xref="MGI:MGI:95390"
                     /translation="MEEKDSKPSETAAEAQRQPEPSSGGGSGGGSSPSDSDTGRRRAL
                     MLPEVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGARGGEGGAGTTE
                     GGGGGAGGAEQLLGARESRPNPACAPSAGGTLSAAAGDPAVDGEGGSKTLSLHGGAKK
                     PGDPGGSLDGVLKARGLGGGDLSVSSDSDSSQASATLGAQPMLWPAWVYCTRYSDRPS
                     SGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNE
                     SQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE"
     exon            1490..3561
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3550
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
ORIGIN      
        1 agagtgaaag tgtctcaggt tgcgctaagt ggcctgaaat tttcccgagc ccgcgcgaaa
       61 gccgaggcag cgaaggcgcg cggaccggga gggagggagc gcaggaggcc ctgcccggcc
      121 cggcctcccg cccctcactc atgcggctcg ggccgcgggg ctgcgcgggg ctcagcgggg
      181 gcgcagccgc tcgcccgtgg ggcgccccgg cccgcagcgg ggcggcggcc tggcgcgggg
      241 agccccgggg cgggcggagc gcagcgcggg cccacggccc tccatgtgcg tgctggcggc
      301 tgcgggcgcg ctgagaaccc gcgcccagca gcctcgaggg tcggccggga ccggcgggtg
      361 gcaacggccg agcagtggct gcccgagcgg gcccgagcgg cagtgagcgc gggcggcgcg
      421 ctgcctgcat gcgcgcggca gcggcggagg cggcggcgcc ggagcccgag gctgctgacc
      481 accaaaagga gcgcggagtt gcacggacgt cccgggtccc cggagtggtg cggtgaccat
      541 ctagagtccg gtgcgtcgcc aagaccggga cccgcttccg ttgaatcgcc cggcgtccct
      601 gccggcgggg agactggtga tttgctaacg cactccgtgg atccgaacgt ggcttggctc
      661 cgagcgcgcc cggcgacctg taggactcca gccctggcca ctgtcgccgc tcgcacgctc
      721 ggaggactcg gctgcgtagg agcgctcggg gctcgctgtg tgccgcggat cggccggaga
      781 tcgctttggc ggtgggccgg tggagaggcg atttgggatt ttctgtgaag tatggaggag
      841 aaggattcca agcccagcga gacggcggcg gaggcgcaga gacagccgga acccagctcc
      901 ggcggcggct ctggcggcgg cagcagcccg agcgactcgg acaccggccg ccggcgggct
      961 ctgatgctgc ccgaggtcct acaggcgcca ggcaaccacc agcatccaca tcgcatcacc
     1021 aacttcttca tcgataacat cctgcggcct gagtttggcc gccgaaagga cgcggggact
     1081 tgctgtgcgg gcgcgggcgg agccagggga ggcgaaggcg gcgctggcac taccgaagga
     1141 ggcggcggcg gcgcaggcgg agccgagcag ctactgggcg ccagggagtc ccgaccgaac
     1201 ccagcgtgcg cacccagcgc gggaggaacg ctctccgccg ccgctggcga ccctgcggtc
     1261 gacggagaag gaggttccaa gacgctatca cttcacggtg gtgccaaaaa acccggcgat
     1321 cctgggggtt ccttggatgg agtgctcaaa gcccggggct tgggcggcgg tgacctgtcg
     1381 gtgagctccg actcggacag ctctcaagcc agcgccactc tgggcgcgca gcccatgctc
     1441 tggcccgctt gggtctactg cacgcgctat tctgaccggc cttcttcagg tcccaggtcc
     1501 cgaaaaccaa agaagaagaa ccctaacaaa gaggacaagc ggcctcgcac agccttcact
     1561 gctgagcagc tccagaggct caaggctgag tttcagacca acaggtacct gacagagcag
     1621 cggcgccaga gtctggcaca ggagctcagc ctgaacgagt ctcagatcaa gatttggttc
     1681 cagaacaagc gggccaaaat caagaaagcc acgggcaaca agaacacttt ggcggtgcac
     1741 ctcatggcac agggcctgta caaccattcc accacggcca aggagggcaa gtcggacagc
     1801 gagtagggcg agcagggcgg gtgggccggc cggccggcca ggtctagggc cgcgagaaac
     1861 aatgcaataa ttttaaaacc gtgaacaaaa ggccagtgta taaagattat accagcatta
     1921 tctgtgaaaa tccagtgtat tagctatggt tctacaataa tctatgtaca tataatttac
     1981 aggtggtgta aaatccaaaa atctgactat aaaatatttt tgagtttttt ttttgtgttt
     2041 cagaggttat gctaatttta tgttattatt atttttctgc aaaggggact gtttagggtt
     2101 tcagatcttt ctttcatttt tcttttctcc ctttttttaa tcccttaaat tccattatgg
     2161 gacattggac acttcttccc ccgaaagaaa aaaaatttaa aacaacttgc tgaagtccaa
     2221 agatttttat tgctgcattt cacaggactg tgaaccgaat aaatagctcc tatttggtct
     2281 gtgggctctg ccgcttgctt tgtgctggct cggacagcac tgtaggaagc gccagccaga
     2341 gtggtgatgg agagctctgg tggggagaga gggcgcagtt ctttgctgag gtctgagaaa
     2401 gtccttgcag gccagcagct ctcccggaac ctctccgcct gcttcctacg cagcctctct
     2461 tctcaaccat ctcagaggcc cgtgctggca gccaccgtgt gtctgcgtct gtgtcgatga
     2521 cccctgtccg ccacagcccc gtagcgacct gccagcgcca actctcccgc caccgtggcc
     2581 agcgttgggg cctggggagc agtgggagtg tgtcctgaag gcttgaaggc tttgctgccg
     2641 ggacagctag ccctgaccca aaagccctca ccgagatagg ccttggggcc tggcagagcg
     2701 agttggaatc atcttccttt cctttttctt ccctcttttt tctttttcat ttatttttga
     2761 atggtaaata catgtatgtg tacatacttg tgtatgcatg tgcacgcaca ccccaggcct
     2821 gtgtgaagct ggggttctgg agctcacagc taccttgact ttcaaaggaa ctcgaatcct
     2881 cccgaatcta gaggacatgt gtaaataatc atttcttatc atgtttttgt cttttttgtt
     2941 tttttttaaa gaaaatatac attttatttt tgaaggtgtg gtactgtgta aattaaatat
     3001 attcaatata tccttccaag tatatatata tatatatata tatatatata tatatatata
     3061 tatatacaaa aaacacatta tatgtaactc cacaatgtct tctgtttagt gtatgggaaa
     3121 aggtgtccaa acatccacct ggggccagca cagtggccag cgtgttctca ggctgcctgc
     3181 tgaggaccga tggtggattt ctctctttgc cttaaacctc tttatttcac tgcgccaaca
     3241 ctttctcttt gtacatattc gtgccaacct tctgaaaagg aagctataaa aacagaagtt
     3301 gtaatttaag agtctgtgaa taaccatctg ctgcttgggg gtcggtggaa cacatgcctt
     3361 ttagcaggaa gcaaatctgt ccttttttaa gtttataaaa tgtgcatttt acagtatcaa
     3421 atatttataa tcttatgaga aatgggtgaa tgttccctac ttaaaactgt ggttatcaaa
     3481 attgtgacca ttccaatctg tgaatttttg tgtgtttaaa ttcctgaaag ttacattaaa
     3541 taaaaagaaa taactcttta aaaaaaaaaa aaaaaa
//
