LOCUS       NM_001395060            3729 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus engrailed 2 (En2), transcript variant 2, mRNA.
ACCESSION   NM_001395060
VERSION     NM_001395060.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Trinh,L.T., Finnel,R.R., Osipovich,A.B., Musselman,J.R.,
            Sampson,L.L., Wright,C.V.E. and Magnuson,M.A.
  TITLE     Positive autoregulation of Sox17 is necessary for gallbladder and
            extrahepatic bile duct formation
  JOURNAL   Development 152 (2) (2025)
   PUBMED   39745200
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Mattioni,L., Barbieri,A., Grigoli,A., Balasco,L., Bozzi,Y. and
            Provenzano,G.
  TITLE     Alterations of Perineuronal Net Expression and Abnormal Social
            Behavior and Whisker-dependent Texture Discrimination in Mice
            Lacking the Autism Candidate Gene Engrailed 2
  JOURNAL   Neuroscience 546, 63-74 (2024)
   PUBMED   38537894
  REMARK    GeneRIF: Alterations of Perineuronal Net Expression and Abnormal
            Social Behavior and Whisker-dependent Texture Discrimination in
            Mice Lacking the Autism Candidate Gene Engrailed 2.
REFERENCE   3  (bases 1 to 3729)
  AUTHORS   Lazzarini,G., Gatta,A., Miragliotta,V., Vaglini,F., Viaggi,C. and
            Pirone,A.
  TITLE     Glial cells are affected more than interneurons by the loss of
            Engrailed 2 gene in the mouse cerebellum
  JOURNAL   J Anat 244 (4), 667-675 (2024)
   PUBMED   38009365
  REMARK    GeneRIF: Glial cells are affected more than interneurons by the
            loss of Engrailed 2 gene in the mouse cerebellum.
REFERENCE   4  (bases 1 to 3729)
  AUTHORS   Song,X., Hu,Q., Xu,X. and Pan,W.
  TITLE     Protein kinase C beta relieves autism-like behavior in EN2 knockout
            mice via upregulation of the FTO/PGC-1alpha/UCP1 axis
  JOURNAL   J Biochem Mol Toxicol 37 (1), e23236 (2023)
   PUBMED   36239013
  REMARK    GeneRIF: Protein kinase C beta relieves autism-like behavior in EN2
            knockout mice via upregulation of the FTO/PGC-1alpha/UCP1 axis.
REFERENCE   5  (bases 1 to 3729)
  AUTHORS   Wang,Y., Herzig,G., Molano,C. and Liu,A.
  TITLE     Differential expression of the Tmem132 family genes in the
            developing mouse nervous system
  JOURNAL   Gene Expr Patterns 45, 119257 (2022)
   PUBMED   35690356
REFERENCE   6  (bases 1 to 3729)
  AUTHORS   Shimamura,K. and Takeichi,M.
  TITLE     Local and transient expression of E-cadherin involved in mouse
            embryonic brain morphogenesis
  JOURNAL   Development 116 (4), 1011-1019 (1992)
   PUBMED   1295725
REFERENCE   7  (bases 1 to 3729)
  AUTHORS   Conlon,R.A. and Rossant,J.
  TITLE     Exogenous retinoic acid rapidly induces anterior ectopic expression
            of murine Hox-2 genes in vivo
  JOURNAL   Development 116 (2), 357-368 (1992)
   PUBMED   1363087
REFERENCE   8  (bases 1 to 3729)
  AUTHORS   Bally-Cuif,L., Alvarado-Mallart,R.M., Darnell,D.K. and Wassef,M.
  TITLE     Relationship between Wnt-1 and En-2 expression domains during early
            development of normal and ectopic met-mesencephalon
  JOURNAL   Development 115 (4), 999-1009 (1992)
   PUBMED   1360404
REFERENCE   9  (bases 1 to 3729)
  AUTHORS   Yamaguchi,T.P., Conlon,R.A. and Rossant,J.
  TITLE     Expression of the fibroblast growth factor receptor FGFR-1/flg
            during gastrulation and segmentation in the mouse embryo
  JOURNAL   Dev Biol 152 (1), 75-88 (1992)
   PUBMED   1321062
REFERENCE   10 (bases 1 to 3729)
  AUTHORS   Wilkie,T.M., Gilbert,D.J., Olsen,A.S., Chen,X.N., Amatruda,T.T.,
            Korenberg,J.R., Trask,B.J., de Jong,P., Reed,R.R., Simon,M.I. et
            al.
  TITLE     Evolution of the mammalian G protein alpha subunit multigene family
  JOURNAL   Nat Genet 1 (2), 85-91 (1992)
   PUBMED   1302014
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129184.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927934.6699686.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164136, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1489              AC129184.3         122405-123893       c
            1490-1650           AC129184.3         121291-121451       c
            1651-3729           AC129184.3         117392-119470       c
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 13.94 cM"
     gene            1..3729
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /note="engrailed 2"
                     /db_xref="GeneID:13799"
                     /db_xref="MGI:MGI:95390"
     exon            1..1489
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             832..1773
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     homeobox protein engrailed-2; homeobox protein en-2"
                     /codon_start=1
                     /product="homeobox protein engrailed-2 isoform 2"
                     /protein_id="NP_001381989.1"
                     /db_xref="GeneID:13799"
                     /db_xref="MGI:MGI:95390"
                     /translation="MEEKDSKPSETAAEAQRQPEPSSGGGSGGGSSPSDSDTGRRRAL
                     MLPEVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGARGGEGGAGTTE
                     GGGGGAGGAEQLLGARESRPNPACAPSAGGTLSAAAGDPAVDGEGGSKTLSLHGGAKK
                     PGDPGGSLDGVLKARGLGGGDLSVSSDSDSSQASATLGAQPMLWPAWVYCTRYSDRPS
                     SGSQSRLALSRARLCEEPASESAPYQALAALGILHDGPRGSPHSFPRLNEIPFLTVPG
                     PENQRRRTLTKRTSGLAQPSLLSSSRGSRLSFRPTGT"
     exon            1490..1650
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..3729
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3706..3711
                     /regulatory_class="polyA_signal_sequence"
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /note="hexamer: AAGAAA"
     polyA_site      3711
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
     polyA_site      3729
                     /gene="En2"
                     /gene_synonym="En-2; mo-En-2"
                     /note="major polyA site"
ORIGIN      
        1 agagtgaaag tgtctcaggt tgcgctaagt ggcctgaaat tttcccgagc ccgcgcgaaa
       61 gccgaggcag cgaaggcgcg cggaccggga gggagggagc gcaggaggcc ctgcccggcc
      121 cggcctcccg cccctcactc atgcggctcg ggccgcgggg ctgcgcgggg ctcagcgggg
      181 gcgcagccgc tcgcccgtgg ggcgccccgg cccgcagcgg ggcggcggcc tggcgcgggg
      241 agccccgggg cgggcggagc gcagcgcggg cccacggccc tccatgtgcg tgctggcggc
      301 tgcgggcgcg ctgagaaccc gcgcccagca gcctcgaggg tcggccggga ccggcgggtg
      361 gcaacggccg agcagtggct gcccgagcgg gcccgagcgg cagtgagcgc gggcggcgcg
      421 ctgcctgcat gcgcgcggca gcggcggagg cggcggcgcc ggagcccgag gctgctgacc
      481 accaaaagga gcgcggagtt gcacggacgt cccgggtccc cggagtggtg cggtgaccat
      541 ctagagtccg gtgcgtcgcc aagaccggga cccgcttccg ttgaatcgcc cggcgtccct
      601 gccggcgggg agactggtga tttgctaacg cactccgtgg atccgaacgt ggcttggctc
      661 cgagcgcgcc cggcgacctg taggactcca gccctggcca ctgtcgccgc tcgcacgctc
      721 ggaggactcg gctgcgtagg agcgctcggg gctcgctgtg tgccgcggat cggccggaga
      781 tcgctttggc ggtgggccgg tggagaggcg atttgggatt ttctgtgaag tatggaggag
      841 aaggattcca agcccagcga gacggcggcg gaggcgcaga gacagccgga acccagctcc
      901 ggcggcggct ctggcggcgg cagcagcccg agcgactcgg acaccggccg ccggcgggct
      961 ctgatgctgc ccgaggtcct acaggcgcca ggcaaccacc agcatccaca tcgcatcacc
     1021 aacttcttca tcgataacat cctgcggcct gagtttggcc gccgaaagga cgcggggact
     1081 tgctgtgcgg gcgcgggcgg agccagggga ggcgaaggcg gcgctggcac taccgaagga
     1141 ggcggcggcg gcgcaggcgg agccgagcag ctactgggcg ccagggagtc ccgaccgaac
     1201 ccagcgtgcg cacccagcgc gggaggaacg ctctccgccg ccgctggcga ccctgcggtc
     1261 gacggagaag gaggttccaa gacgctatca cttcacggtg gtgccaaaaa acccggcgat
     1321 cctgggggtt ccttggatgg agtgctcaaa gcccggggct tgggcggcgg tgacctgtcg
     1381 gtgagctccg actcggacag ctctcaagcc agcgccactc tgggcgcgca gcccatgctc
     1441 tggcccgctt gggtctactg cacgcgctat tctgaccggc cttcttcagg ctcacaaagc
     1501 cgactggcct tgagtcgcgc tcggctttgt gaggagccag cgagtgagtc cgcgccatac
     1561 caagccctgg cggccctggg gatcttgcat gatggcccgc gtggttcgcc tcacagcttt
     1621 cctaggctta acgaaatccc tttcctgact gtcccaggtc ccgaaaacca aagaagaaga
     1681 accctaacaa agaggacaag cggcctcgca cagccttcac tgctgagcag ctccagaggc
     1741 tcaaggctga gtttcagacc aacaggtacc tgacagagca gcggcgccag agtctggcac
     1801 aggagctcag cctgaacgag tctcagatca agatttggtt ccagaacaag cgggccaaaa
     1861 tcaagaaagc cacgggcaac aagaacactt tggcggtgca cctcatggca cagggcctgt
     1921 acaaccattc caccacggcc aaggagggca agtcggacag cgagtagggc gagcagggcg
     1981 ggtgggccgg ccggccggcc aggtctaggg ccgcgagaaa caatgcaata attttaaaac
     2041 cgtgaacaaa aggccagtgt ataaagatta taccagcatt atctgtgaaa atccagtgta
     2101 ttagctatgg ttctacaata atctatgtac atataattta caggtggtgt aaaatccaaa
     2161 aatctgacta taaaatattt ttgagttttt tttttgtgtt tcagaggtta tgctaatttt
     2221 atgttattat tatttttctg caaaggggac tgtttagggt ttcagatctt tctttcattt
     2281 ttcttttctc ccttttttta atcccttaaa ttccattatg ggacattgga cacttcttcc
     2341 cccgaaagaa aaaaaattta aaacaacttg ctgaagtcca aagattttta ttgctgcatt
     2401 tcacaggact gtgaaccgaa taaatagctc ctatttggtc tgtgggctct gccgcttgct
     2461 ttgtgctggc tcggacagca ctgtaggaag cgccagccag agtggtgatg gagagctctg
     2521 gtggggagag agggcgcagt tctttgctga ggtctgagaa agtccttgca ggccagcagc
     2581 tctcccggaa cctctccgcc tgcttcctac gcagcctctc ttctcaacca tctcagaggc
     2641 ccgtgctggc agccaccgtg tgtctgcgtc tgtgtcgatg acccctgtcc gccacagccc
     2701 cgtagcgacc tgccagcgcc aactctcccg ccaccgtggc cagcgttggg gcctggggag
     2761 cagtgggagt gtgtcctgaa ggcttgaagg ctttgctgcc gggacagcta gccctgaccc
     2821 aaaagccctc accgagatag gccttggggc ctggcagagc gagttggaat catcttcctt
     2881 tcctttttct tccctctttt ttctttttca tttatttttg aatggtaaat acatgtatgt
     2941 gtacatactt gtgtatgcat gtgcacgcac accccaggcc tgtgtgaagc tggggttctg
     3001 gagctcacag ctaccttgac tttcaaagga actcgaatcc tcccgaatct agaggacatg
     3061 tgtaaataat catttcttat catgtttttg tcttttttgt ttttttttaa agaaaatata
     3121 cattttattt ttgaaggtgt ggtactgtgt aaattaaata tattcaatat atccttccaa
     3181 gtatatatat atatatatat atatatatat atatatatat atatatacaa aaaacacatt
     3241 atatgtaact ccacaatgtc ttctgtttag tgtatgggaa aaggtgtcca aacatccacc
     3301 tggggccagc acagtggcca gcgtgttctc aggctgcctg ctgaggaccg atggtggatt
     3361 tctctctttg ccttaaacct ctttatttca ctgcgccaac actttctctt tgtacatatt
     3421 cgtgccaacc ttctgaaaag gaagctataa aaacagaagt tgtaatttaa gagtctgtga
     3481 ataaccatct gctgcttggg ggtcggtgga acacatgcct tttagcagga agcaaatctg
     3541 tcctttttta agtttataaa atgtgcattt tacagtatca aatatttata atcttatgag
     3601 aaatgggtga atgttcccta cttaaaactg tggttatcaa aattgtgacc attccaatct
     3661 gtgaattttt gtgtgtttaa attcctgaaa gttacattaa ataaaaagaa ataactcttt
     3721 aatataaaa
//
