LOCUS       NM_001347582            2984 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus echinoderm microtubule associated protein like 2
            (Eml2), transcript variant 3, mRNA.
ACCESSION   NM_001347582 XM_011250713
VERSION     NM_001347582.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Chai,G., Webb,A., Li,C., Antaki,D., Lee,S., Breuss,M.W., Lang,N.,
            Stanley,V., Anzenberg,P., Yang,X., Marshall,T., Gaffney,P.,
            Wierenga,K.J., Chung,B.H., Tsang,M.H., Pais,L.S., Lovgren,A.K.,
            VanNoy,G.E., Rehm,H.L., Mirzaa,G., Leon,E., Diaz,J., Neumann,A.,
            Kalverda,A.P., Manfield,I.W., Parry,D.A., Logan,C.V., Johnson,C.A.,
            Bonthron,D.T., Valleley,E.M.A., Issa,M.Y., Abdel-Ghafar,S.F.,
            Abdel-Hamid,M.S., Jennings,P., Zaki,M.S., Sheridan,E. and
            Gleeson,J.G.
  TITLE     Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause
            Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly
  JOURNAL   Neuron 109 (2), 241-256 (2021)
   PUBMED   33220177
REFERENCE   3  (bases 1 to 2984)
  AUTHORS   Jiang,D., Burger,C.A., Casasent,A.K., Albrecht,N.E., Li,F. and
            Samuel,M.A.
  TITLE     Spatiotemporal gene expression patterns reveal molecular
            relatedness between retinal laminae
  JOURNAL   J Comp Neurol 528 (5), 729-755 (2020)
   PUBMED   31609468
REFERENCE   4  (bases 1 to 2984)
  AUTHORS   Aryal,S., Anand,D., Hernandez,F.G., Weatherbee,B.A.T., Huang,H.,
            Reddy,A.P., Wilmarth,P.A., David,L.L. and Lachke,S.A.
  TITLE     MS/MS in silico subtraction-based proteomic profiling as an
            approach to facilitate disease gene discovery: application to lens
            development and cataract
  JOURNAL   Hum Genet 139 (2), 151-184 (2020)
   PUBMED   31797049
REFERENCE   5  (bases 1 to 2984)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 2984)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 2984)
  AUTHORS   Terami,H., Hidaka,K., Shirai,M., Narumiya,H., Kuroyanagi,T.,
            Arai,Y., Aburatani,H. and Morisaki,T.
  TITLE     Efficient capture of cardiogenesis-associated genes expressed in ES
            cells
  JOURNAL   Biochem Biophys Res Commun 355 (1), 47-53 (2007)
   PUBMED   17286962
REFERENCE   8  (bases 1 to 2984)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC145199.3.
            
            On Feb 20, 2023 this sequence version replaced NM_001347582.1.
            
            Transcript Variant: This variant (3) encodes the longest isoform
            (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.689539.1,
                                           SRR17784647.1357683.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-337               AC145199.3         33101-33437         c
            338-556             AC145199.3         30417-30635         c
            557-680             AC145199.3         30213-30336         c
            681-785             AC145199.3         30007-30111         c
            786-814             AC145199.3         28125-28153         c
            815-944             AC145199.3         27463-27592         c
            945-1094            AC145199.3         24907-25056         c
            1095-1164           AC145199.3         23691-23760         c
            1165-1275           AC145199.3         23491-23601         c
            1276-1371           AC145199.3         19049-19144         c
            1372-1506           AC145199.3         18144-18278         c
            1507-1606           AC145199.3         15754-15853         c
            1607-1761           AC145199.3         13511-13665         c
            1762-1887           AC145199.3         13244-13369         c
            1888-2019           AC145199.3         9231-9362           c
            2020-2087           AC145199.3         8671-8738           c
            2088-2176           AC145199.3         8209-8297           c
            2177-2274           AC145199.3         7935-8032           c
            2275-2362           AC145199.3         7266-7353           c
            2363-2458           AC145199.3         6790-6885           c
            2459-2589           AC145199.3         5830-5960           c
            2590-2984           AC145199.3         3382-3776           c
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 9.46 cM"
     gene            1..2984
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /note="echinoderm microtubule associated protein like 2"
                     /db_xref="GeneID:72205"
                     /db_xref="MGI:MGI:1919455"
     exon            1..337
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..2715
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /note="isoform 3 is encoded by transcript variant 3"
                     /codon_start=1
                     /product="echinoderm microtubule-associated protein-like 2
                     isoform 3"
                     /protein_id="NP_001334511.1"
                     /db_xref="CCDS:CCDS85223.1"
                     /db_xref="GeneID:72205"
                     /db_xref="MGI:MGI:1919455"
                     /translation="MATGGRAWGGDRARAGAAGAGGGCGGAMAERGPAFCGLYDTSSL
                     LQYCNDDNLSGTSGMEVDDRVSALEQRLQLQEDELAVLKAALADALRRLRACEEQGAA
                     LRARSTPKGRAPPRLGTTASVCQLLKGLPTRTPLNGSGPPRRVGGYATSPSSPKKEAS
                     SGRSSRRYLSPERLASVRREDPRSRTTSSSSNCSAKKEGKTKEVIFSMEEGSVKMFLR
                     GRPVPMLIPDELAPTYSLDTRSELPSSRLKLDWVYGYRGRDCRANLYLLPTGEVVYFV
                     ASVAVLYSVEEQRQRHYLGHNDDIKCLAVHPDMVTIATGQVAGTTKEGKPLPPHVRVW
                     DSVSLSTLHVLGLGVFDRAVCCVAFSKSNGGNLLCAVDESNDHVLSVWDWAKESKVVD
                     SKCSNEAVLVATFHPTDPSLLITCGKSHIYFWSLEGGSLSKRQGLFEKHEKPKYVLCV
                     TFLEGGDVVTGDSGGNLYVWGKGGNRITQEVQGAHDGGVFALCALRDGTLVSGGGRDR
                     RVVLWGSDYSKVQEVEVPEDFGPVRTVAEGRGDTLYVGTTRNSILLGSVHTGFSLLVQ
                     GHVEELWGLATHPSRAQFVTCGQDKLVHLWSSETHQPVWSRSIEDPARSAGFHPSGSV
                     LAVGTVTGRWLLLDTETHDLVAIHTDGNEQISVVSFSPDGAYLAVGSHDNLVYVYTVD
                     QGGRKVSRLGKCSGHSSFITHLDWAQDSTCFVTNSGDYEILYWDPVTCKQITSADTVR
                     NVEWATATCVLGFGVFGIWPEGADGTDINAVARSHDGKLLVSADDFGKVHLFSYPCCQ
                     PRALSHKYGGHSSHVTNVAFLWDDSMALTTGGKDTSVLQWRVA"
     exon            338..556
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            557..680
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            681..785
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            786..814
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            815..944
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1094
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1164
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1275
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1371
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1506
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..1606
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1761
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1887
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2019
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2087
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2176
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2274
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2362
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2458
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2459..2589
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2590..2984
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2965..2970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /note="hexamer: AATACA"
     polyA_site      2984
                     /gene="Eml2"
                     /gene_synonym="1600029N02Rik; EMAP-2"
                     /note="major polyA site"
ORIGIN      
        1 agtacctagg gggaccccga cgcagccaat cctgacctga agactgttgc gggggttggg
       61 ggaacggaca gagcagtctc tcgacaccct tacagggcat cctaggggat gacccctgtg
      121 ccaggctcag agccggccgc ctgatgctgg gtgggccgtg ctggggatgc tggaacgaag
      181 ggcgttgcta tggcaacggg aggcagggcc tggggggggg accgggcccg agctggggct
      241 gcgggggccg gcggtggctg tggtggggca atggcggagc gcggcccggc cttctgcggc
      301 ctctacgaca cgtcctctct gctgcaatac tgcaacgatg ataatctgtc cggcaccagt
      361 ggcatggagg tggacgaccg cgtgtcggcg ctggagcagc ggctgcagct gcaagaagat
      421 gaactggcgg tcctaaaggc ggcgctggcg gacgcgctgc gtcgcctgcg ggcatgcgaa
      481 gagcaaggcg cggcgctgcg tgcgaggagc acccctaagg gccgggcgcc cccgcgtctg
      541 ggcaccaccg cctcggtgtg tcagctcctg aaaggccttc ccaccaggac gcccctcaat
      601 ggctccggac ccccgcgacg cgtgggcggc tatgccacct ccccatcttc ccccaagaaa
      661 gaggcgagct ctggccgtag cagccgtcgc tacttatcac ctgagcgcct ggcctctgtg
      721 cgtcgcgagg acccccgtag ccgtaccaca tcttccagta gtaattgtag cgccaaaaaa
      781 gaagggaaaa ccaaagaagt tatcttcagc atggaggagg gatccgtaaa gatgtttctg
      841 cggggacgcc ctgtgcccat gctgatcccg gacgaactgg cgcccaccta tagcctggac
      901 actcgttctg agctgccctc cagccggcta aagctagact gggtctatgg ctatcgaggc
      961 cgggactgca gggccaacct ttacctcctg cccactgggg aggtggtgta ctttgtggcc
     1021 tctgtggccg tactgtacag cgtggaggag cagagacaac gacactacct ggggcacaac
     1081 gatgacatca aatgtctggc tgtccatcca gatatggtca ccatcgccac aggacaggtg
     1141 gcaggcacca caaaggaagg caagcctctg ccaccccatg tgcgtgtgtg ggactcggtt
     1201 tccctttcca ccttacacgt cctgggcctg ggggtgtttg acagagctgt gtgctgtgtg
     1261 gccttctcca aatccaatgg gggcaacctg ctatgtgcag tggacgaatc caacgaccat
     1321 gtgctctctg tgtgggactg ggccaaggag agcaaagtgg tggatagcaa gtgctctaac
     1381 gaggcagtgc tagtggccac cttccacccc actgatccca gcctgctgat aacctgtggc
     1441 aaatcccaca tctacttctg gagcctggag ggaggcagtc tgagcaagag gcagggcctg
     1501 ttcgagaaac acgagaaacc aaagtacgtg ctgtgtgtga ccttcctgga aggtggcgat
     1561 gtggtcactg gagactctgg ggggaacctc tatgtctggg gcaaaggtgg aaaccgcatc
     1621 acccaggaag ttcaaggagc ccatgatggt ggcgtgtttg ctctctgcgc cctgcgggac
     1681 gggacgctgg tgtctggagg gggccgcgat cgtagggtgg tcctctgggg ttcggactac
     1741 agcaaggtgc aggaggtgga ggtcccagag gacttcggcc ctgtacgcac cgtggcagag
     1801 ggccggggag acacactgta cgtggggacc acccgtaact ccatcttgct aggctcggtg
     1861 cacacagggt tttcgctgct tgtccaggga catgtggagg agctgtgggg cctggccaca
     1921 catcctagca gggcacagtt tgtgacctgt gggcaggaca agctggtgca tctgtggagt
     1981 tccgagaccc accagccagt gtggagcagg agcatcgagg accctgcccg ttcggctggc
     2041 ttccacccca gtggctccgt cctagcggtg ggcacagtga ccggcaggtg gctgctactg
     2101 gacacagaga cccatgacct ggtggctatc cacacagatg gcaatgagca gatctccgtg
     2161 gtcagctttt caccagacgg ggcgtacctg gctgtgggct cccacgacaa cttggtgtac
     2221 gtgtacacgg tggaccaggg tggccgcaag gtcagccgcc tgggcaagtg ctcgggccat
     2281 tccagtttta tcacccacct ggattgggcc caggacagca cctgctttgt caccaactct
     2341 ggagactacg agattctgta ctgggacccc gtcacctgta agcaaatcac cagtgcagac
     2401 accgtgagaa acgtggaatg ggctaccgcc acttgtgtgt tgggttttgg agtgtttggg
     2461 atttggcccg agggagcaga tggcacagac atcaatgctg tggcccgctc tcatgatggg
     2521 aagttgctgg tttctgctga cgactttggc aaagtgcacc tgttcagcta cccctgctgt
     2581 cagccgagag ccctcagcca caaatacggt ggacacagca gccatgtgac taatgtggcc
     2641 ttcttgtggg atgacagcat ggccctaacc actgggggca aagacacgag cgtgctgcag
     2701 tggcgggtgg cctgaagcag gggtgtgggg ccagggtagc gtcctgtggc agggttgaga
     2761 tctatttttg ggagatgtct attgcggagt acagtaatat atacccagag tatgtctata
     2821 gaagaggggg ttatggggcg ggcgggagca gacagaggtc tctatttatc ggggtgggac
     2881 gaatgggcca ccatgccttg tgggagccat tggtgtttgg tttggggtat ttttttttaa
     2941 gtttattctt ttatatcatt cagaaataca gacttatgca ctga
//
