LOCUS       NM_177752               2247 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus essential meiotic structure-specific endonuclease 1
            (Eme1), transcript variant 2, mRNA.
ACCESSION   NM_177752
VERSION     NM_177752.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2247)
  AUTHORS   Ulmke,P.A., Sakib,M.S., Ditte,P., Sokpor,G., Kerimoglu,C., Pham,L.,
            Xie,Y., Mao,X., Rosenbusch,J., Teichmann,U., Nguyen,H.P.,
            Fischer,A., Eichele,G., Staiger,J.F. and Tuoc,T.
  TITLE     Molecular Profiling Reveals Involvement of ESCO2 in Intermediate
            Progenitor Cell Maintenance in the Developing Mouse Cortex
  JOURNAL   Stem Cell Reports 16 (4), 968-984 (2021)
   PUBMED   33798452
REFERENCE   4  (bases 1 to 2247)
  AUTHORS   Simonson,L., Vold,S., Mowers,C., Massey,R.J., Ong,I.M.,
            Longley,B.J. and Chang,H.
  TITLE     Keratin 13 deficiency causes white sponge nevus in mice
  JOURNAL   Dev Biol 468 (1-2), 146-153 (2020)
   PUBMED   32758484
REFERENCE   5  (bases 1 to 2247)
  AUTHORS   Toledo,M., Sun,X., Brieno-Enriquez,M.A., Raghavan,V., Gray,S.,
            Pea,J., Milano,C.R., Venkatesh,A., Patel,L., Borst,P.L., Alani,E.
            and Cohen,P.E.
  TITLE     A mutation in the endonuclease domain of mouse MLH3 reveals novel
            roles for MutLgamma during crossover formation in meiotic prophase
            I
  JOURNAL   PLoS Genet 15 (6), e1008177 (2019)
   PUBMED   31170160
  REMARK    GeneRIF: Double mutant mice homozygous for the Mlh3DN/DN and
            Mus81-/- mutations show losses in chiasmata close to those observed
            in Mlh3-/- males, indicating that the MUS81-EME1-regulated
            crossover pathway can only partially account for the increased
            residual chiasmata in Mlh3DN/DN spermatocytes
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2247)
  AUTHORS   Albino,D., Brizzolara,A., Moretti,S., Falugi,C., Mirisola,V.,
            Scaruffi,P., Di Candia,M., Truini,M., Coco,S., Bonassi,S. and
            Tonini,G.P.
  TITLE     Gene expression profiling identifies eleven DNA repair genes
            down-regulated during mouse neural crest cell migration
  JOURNAL   Int J Dev Biol 55 (1), 65-72 (2011)
   PUBMED   21425081
REFERENCE   7  (bases 1 to 2247)
  AUTHORS   Mistry,H., Gibson,L., Yun,J.W., Sarras,H., Tamblyn,L. and
            McPherson,J.P.
  TITLE     Interplay between Np95 and Eme1 in the DNA damage response
  JOURNAL   Biochem Biophys Res Commun 375 (3), 321-325 (2008)
   PUBMED   18692478
  REMARK    GeneRIF: These findings link Eme1 with the replication-associated
            chromatin modifier functions of Np95 in the cellular response to
            DNA damage.
REFERENCE   8  (bases 1 to 2247)
  AUTHORS   Chang,J.H., Kim,J.J., Choi,J.M., Lee,J.H. and Cho,Y.
  TITLE     Crystal structure of the Mus81-Eme1 complex
  JOURNAL   Genes Dev 22 (8), 1093-1106 (2008)
   PUBMED   18413719
REFERENCE   9  (bases 1 to 2247)
  AUTHORS   Hanada,K., Budzowska,M., Modesti,M., Maas,A., Wyman,C., Essers,J.
            and Kanaar,R.
  TITLE     The structure-specific endonuclease Mus81-Eme1 promotes conversion
            of interstrand DNA crosslinks into double-strands breaks
  JOURNAL   EMBO J 25 (20), 4921-4932 (2006)
   PUBMED   17036055
  REMARK    GeneRIF: Mus81-Eme1- and Rad54-mediated homologous recombination
            are involved in the same DNA replication-dependent interstrand
            crosslinks repair pathway
REFERENCE   10 (bases 1 to 2247)
  AUTHORS   Abraham,J., Lemmers,B., Hande,M.P., Moynahan,M.E., Chahwan,C.,
            Ciccia,A., Essers,J., Hanada,K., Chahwan,R., Khaw,A.K.,
            McPherson,P., Shehabeldin,A., Laister,R., Arrowsmith,C., Kanaar,R.,
            West,S.C., Jasin,M. and Hakem,R.
  TITLE     Eme1 is involved in DNA damage processing and maintenance of
            genomic stability in mammalian cells
  JOURNAL   EMBO J 22 (22), 6137-6147 (2003)
   PUBMED   14609959
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645764.15.
            
            On Jul 6, 2018 this sequence version replaced NM_177752.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089459.1, AK078516.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AL645764.15        71261-71308         c
            49-849              AL645764.15        67788-68588         c
            850-980             AL645764.15        67573-67703         c
            981-1067            AL645764.15        65708-65794         c
            1068-1186           AL645764.15        65509-65627         c
            1187-1307           AL645764.15        65193-65313         c
            1308-1423           AL645764.15        64856-64971         c
            1424-1613           AL645764.15        63381-63570         c
            1614-2247           AL645764.15        62537-63170         c
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 58.9 cM"
     gene            1..2247
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /note="essential meiotic structure-specific endonuclease
                     1"
                     /db_xref="GeneID:268465"
                     /db_xref="MGI:MGI:3576783"
     exon            1..48
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     exon            49..849
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1790
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /note="isoform 2 is encoded by transcript variant 2;
                     crossover junction endonuclease EME1; essential meiotic
                     endonuclease 1 homolog 1"
                     /codon_start=1
                     /product="crossover junction endonuclease EME1 isoform 2"
                     /protein_id="NP_808420.1"
                     /db_xref="CCDS:CCDS25262.1"
                     /db_xref="GeneID:268465"
                     /db_xref="MGI:MGI:3576783"
                     /translation="MALRRLSLSRLSTESDSEDLPTFAFLKKEPSSTNRKPPQRAKNI
                     VVVTSDSEASCPPSPGLKGPPCVPSAAGAPPQAGPVRVLSSSSEDEDVFVPLAERITC
                     KLLTSKQLCPELSSSSLKTGLDGQNNASAPCDWKRQPWPKIPDVPLHGALEKSAANDE
                     DSLLDDQCRQLPTYQATCRELAVSKTNSDRPLPKKRTKHIQTVQSGGSQGCWRPGQAS
                     RKENTPRQHERKKKAEMIKRLKAQRPEECLKHIVVVLDPVLLQMEGGGQLLGALQAME
                     CSCVIEVQAIPRSITWRRRRTELVEDGDDWMEEPTILVLVLAEVFMSMAYNLKQASPS
                     STEKGKETLRSFVTDVTAKTGKALSLVIVDQEKCFRPQNPPRRRKSGMANKQAKAKHQ
                     QRQESSTGLMVSRADMEKALVDLQLYTEAQAWMVQSWKELADFTCAFTKAVAEAPFKK
                     LRDQVTFSFFLEKDWAGGMKVDQSGRGLALIWRRQIQQLNRVSSEMASAIVDAYPSPQ
                     LLVQAYQRCFSEQERQNLLADIQVRRGEGVTATSRRVGPELSRRIYLQMTTAQPDLIL
                     DSVD"
     exon            850..980
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1067
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1186
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1307
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1423
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1613
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..2247
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2201..2206
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /note="hexamer: AATAAA"
     polyA_site      2216
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
     polyA_site      2223
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
                     /note="major polyA site"
     polyA_site      2247
                     /gene="Eme1"
                     /gene_synonym="6820428D13"
ORIGIN      
        1 acttccgggt cttgcggaca attcaaagag tggcgggagc agtagcaggc agttcatttg
       61 agagctctaa tgggttgatg gctctaagaa ggttatccct gtctcgcctc tccacggaga
      121 gtgattctga ggacctgccc acatttgcct ttttgaagaa ggaaccatct tcaaccaaca
      181 ggaagccacc tcagagggcg aagaacatag tggttgtcac ctcagattct gaagcctcct
      241 gtcctccatc accaggcctc aaaggtccac catgtgtccc cagtgcagct ggagctcccc
      301 cacaagcagg gccagtcaga gtgctaagca gtagcagtga ggacgaagat gtatttgtcc
      361 ccctagctga gaggatcaca tgtaagcttt tgacgagcaa gcagctgtgc cctgagctct
      421 ctagctcctc acttaaaaca ggtttggatg gccaaaataa tgccagcgca ccatgtgact
      481 ggaaaaggca accatggcca aagattcccg atgttcccct ccacggtgcc ttagagaaga
      541 gtgctgcaaa tgatgaggac tctctcttag acgatcagtg tcgtcagctt ccaacctacc
      601 aggctacctg cagggagctg gcagtctcca aaacaaattc cgaccgacct ctacccaaga
      661 agagaaccaa gcatattcag acggtccaga gcggaggctc tcagggatgc tggcgaccgg
      721 gacaggcaag caggaaggaa aacaccccga ggcagcatga aagaaaaaag aaggcagaga
      781 tgatcaagag gctcaaagcc cagaggccag aggaatgcct gaagcacatc gtggtggtgc
      841 tggatccagt gcttttacag atggaaggtg ggggccagct cctgggagcg ctgcaggcca
      901 tggagtgcag ctgtgtgatt gaagtccagg ccatacctcg aagcatcact tggaggagga
      961 ggaggacaga gctggtagag gatggagatg actggatgga agagccaaca atcctggtgt
     1021 tggtcctggc agaggtcttc atgtccatgg cctacaactt aaagcaggca agtcctagca
     1081 gcaccgagaa agggaaggag acccttcgga gctttgtaac tgatgtcaca gcaaagacgg
     1141 ggaaagcatt gtcactggtg attgtggacc aggagaaatg cttcaggcct cagaatcccc
     1201 cgaggagaag gaaatcagga atggcaaata aacaggccaa agcgaagcat cagcagaggc
     1261 aagagtctag cacgggactc atggtgtcca gggcagacat ggagaaggca ctggtggatc
     1321 tgcagcttta tacagaagcc caggcgtgga tggtgcagag ctggaaggag ctggcggact
     1381 tcacctgtgc atttacaaag gctgtggctg aagcaccctt caagaagctc cgggatcaag
     1441 tcaccttttc cttcttcctg gagaaagact gggctggagg gatgaaagtg gaccagtccg
     1501 gcaggggact ggcactgatc tggaggcggc agatccagca gctgaaccga gtcagctcgg
     1561 agatggccag tgctattgtg gacgcctatc cctcaccaca gctcctggtg caggcttatc
     1621 agcggtgttt ctctgagcaa gaacgtcaga atttgctggc tgacatacaa gtgcgacgtg
     1681 gggaaggtgt gacagccacc tcccgccgtg ttgggccaga attatccagg cggatctacc
     1741 ttcaaatgac cacagcgcaa ccagatctca tcttagacag tgttgactga tatcccacct
     1801 tggctattga cagtagacag gccagaagta cctccttggt acagttaggg agcaagtctc
     1861 tcatctctgg gaacagagaa ggctgagtca cttgaatcta cctccaccat agcactcatg
     1921 ttcctctccc ggcaagcgag atgccatgga cagccatccc accccatccc agttgaccct
     1981 cacagcacaa aggagaaagg cagaccactc agacatggat ttaataattg tagaaatcca
     2041 agaaataagc atcaaatctc gaagtcagag tgaactcttg cctgcgggtt ggcttgacta
     2101 cgcccagcca ctgagctgcc tcaaccagct agggagctat gatgaggctg actcctgttt
     2161 tcatgatgtc accatatgta tgtagtatgt attttgtctc aataaagcct ttgtaccaat
     2221 ggctgtggag cttggaggaa gactaaa
//
