LOCUS       NM_001163522            1476 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus endomucin (Emcn), transcript variant 1, mRNA.
ACCESSION   NM_001163522
VERSION     NM_001163522.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Chen,X., Tober,J., Dominguez,M., Tang,A.T., Bockman,J., Yang,J.,
            Mani,S., Lee,C.N., Chen,M., Thillaikumaran,T., Mericko-Ishizuka,P.,
            Mainigi,M., Speck,N.A. and Kahn,M.L.
  TITLE     Lineage tracing studies suggest that the placenta is not a de novo
            source of hematopoietic stem cells
  JOURNAL   PLoS Biol 23 (1), e3003003 (2025)
   PUBMED   39874373
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Fan,W., Li,Z., He,X., Wang,X., Sun,M. and Yang,Z.
  TITLE     SLC25A1 regulates placental development to ensure embryonic heart
            morphogenesis
  JOURNAL   Development 151 (22) (2024)
   PUBMED   39591637
REFERENCE   3  (bases 1 to 1476)
  AUTHORS   Brookins,E., Serrano,S.E., Hyder,Z., Yacu,G.S., Finer,G. and
            Thomson,B.R.
  TITLE     Non-endothelial expression of endomucin in the mouse and human
            choroid
  JOURNAL   Exp Eye Res 247, 110054 (2024)
   PUBMED   39153592
  REMARK    GeneRIF: Non-endothelial expression of endomucin in the mouse and
            human choroid.
REFERENCE   4  (bases 1 to 1476)
  AUTHORS   Van Wauwe,J., Mahy,A., Craps,S., Ekhteraei-Tousi,S., Vrancaert,P.,
            Kemps,H., Dheedene,W., Donate Puertas,R., Trenson,S.,
            Roderick,H.L., Beerens,M. and Luttun,A.
  TITLE     PRDM16 determines specification of ventricular cardiomyocytes by
            suppressing alternative cell fates
  JOURNAL   Life Sci Alliance 7 (12), e202402719 (2024)
   PUBMED   39304345
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1476)
  AUTHORS   He,H., Bell,S.M., Davis,A.K., Zhao,S., Sridharan,A., Na,C.L.,
            Guo,M., Xu,Y., Snowball,J., Swarr,D.T., Zacharias,W.J. and
            Whitsett,J.A.
  TITLE     PRDM3/16 regulate chromatin accessibility required for NKX2-1
            mediated alveolar epithelial differentiation and function
  JOURNAL   Nat Commun 15 (1), 8112 (2024)
   PUBMED   39284798
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1476)
  AUTHORS   Brandau,O., Aszodi,A., Hunziker,E.B., Neame,P.J., Vestweber,D. and
            Fassler,R.
  TITLE     Chondromodulin I is dispensable during enchondral ossification and
            eye development
  JOURNAL   Mol Cell Biol 22 (18), 6627-6635 (2002)
   PUBMED   12192060
REFERENCE   7  (bases 1 to 1476)
  AUTHORS   Brachtendorf,G., Kuhn,A., Samulowitz,U., Knorr,R., Gustafsson,E.,
            Potocnik,A.J., Fassler,R. and Vestweber,D.
  TITLE     Early expression of endomucin on endothelium of the mouse embryo
            and on putative hematopoietic clusters in the dorsal aorta
  JOURNAL   Dev Dyn 222 (3), 410-419 (2001)
   PUBMED   11747076
REFERENCE   8  (bases 1 to 1476)
  AUTHORS   Ueno,M., Igarashi,K., Kimura,N., Okita,K., Takizawa,M.,
            Nobuhisa,I., Kojima,T., Kitamura,T., Samulowitz,U., Vestweber,D.,
            Shimomura,T., Suda,T., Nakashima,K. and Taga,T.
  TITLE     Endomucin is expressed in embryonic dorsal aorta and is able to
            inhibit cell adhesion
  JOURNAL   Biochem Biophys Res Commun 287 (2), 501-506 (2001)
   PUBMED   11554756
REFERENCE   9  (bases 1 to 1476)
  AUTHORS   Kinoshita,M., Nakamura,T., Ihara,M., Haraguchi,T., Hiraoka,Y.,
            Tashiro,K. and Noda,M.
  TITLE     Identification of human endomucin-1 and -2 as membrane-bound
            O-sialoglycoproteins with anti-adhesive activity
  JOURNAL   FEBS Lett 499 (1-2), 121-126 (2001)
   PUBMED   11418125
REFERENCE   10 (bases 1 to 1476)
  AUTHORS   Morgan,S.M., Samulowitz,U., Darley,L., Simmons,D.L. and
            Vestweber,D.
  TITLE     Biochemical characterization and molecular cloning of a novel
            endothelial-specific sialomucin
  JOURNAL   Blood 93 (1), 165-175 (1999)
   PUBMED   9864158
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA822842.1 and BC003706.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003706.1, AK029348.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                AA822842.1         1-39
            40-1476             BC003706.1         1-1437
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 63.65 cM"
     gene            1..1476
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /note="endomucin"
                     /db_xref="GeneID:59308"
                     /db_xref="MGI:MGI:1891716"
     exon            1..235
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..948
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; mucin 14, endothelial; endomucin-1; MUC-14;
                     mucin-14; endomucin-1/2"
                     /codon_start=1
                     /product="endomucin isoform 1 precursor"
                     /protein_id="NP_001156994.1"
                     /db_xref="CCDS:CCDS51075.1"
                     /db_xref="GeneID:59308"
                     /db_xref="MGI:MGI:1891716"
                     /translation="MRLLQATVLFFLLSNSLCHSEDGKDVQNDSIPTPAETSTTKASV
                     TIPGIVSVTNPNKPADGTPPEGTTKSDVSQTSLVTTINSLTTPKHEVGTTTEGPLRNE
                     SSTMKITVPNTPTSNANSTLPGSQNKTENQSSIRTTEISVTTQLLDALPKITATSSAS
                     LTTAHTMSLLQDTEDRKIATTPSTTPSYSSIILPVVIALVVITLLVFTLVGLYRICWK
                     RDPGTPENGNDQPQSDKESVKLLTVKTISHESGEHSAQGKTKN"
     exon            236..358
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            359..433
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            434..547
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            548..586
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            587..676
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            677..730
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            731..826
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            827..851
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            852..913
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            914..987
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1461
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1426..1431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /note="hexamer: AATAAA"
     polyA_site      1461
                     /gene="Emcn"
                     /gene_synonym="0610012K22Rik; Muc14"
                     /note="major polyA site"
ORIGIN      
        1 ctctagttct ttatcagaat ccccaaaata agtaggaatg ggcagcgtct gctcacagtc
       61 agtacacctt tcccatttgc taatacggcc tggccaggct gagaggaatc cttccctgcc
      121 ttaccctgga cagtgaagcc actgagacca tcggtaggca ccatgcggct gcttcaagcg
      181 actgttcttt tcttcctttt gtccaacagt ctctgccaca gtgaggacgg caaagatgtt
      241 cagaatgact ctattccaac accggcagaa acttccacaa caaaagcctc tgtcacaata
      301 ccaggcatcg tgtcagtgac aaatcctaac aaaccagcag atggaacacc tcccgaagga
      361 acgaccaaaa gtgacgtatc ccaaacatct ttggtgacaa caattaattc actgacaaca
      421 ccaaaacatg aagtgggaac aacaactgaa ggtcccctaa ggaatgaaag ttccactatg
      481 aaaataacag tgccaaatac tccaacttca aatgctaatt caacgctacc aggttcccag
      541 aacaagactg agaatcagag ttcaatcaga acaacggaaa tatcagttac tactcaacta
      601 ctggatgcct tgccgaaaat cactgcaaca tcctctgcat ctctaacaac tgcacacacc
      661 atgtcactgc ttcaagatac tgaggatagg aagattgcaa ccactccatc aaccaccccc
      721 tcctattcca gtatcatttt gcctgtggtt atcgcgctgg ttgttataac actcttggta
      781 tttactctgg tgggtttgta tcgaatatgc tggaagagag acccaggcac tccagaaaac
      841 ggaaatgatc agcctcagtc cgataaagag agtgtgaagc tgctcactgt taagacaatc
      901 tctcatgagt ctggcgaaca ctctgcacag gggaaaacca agaactgaca tcgtgatgga
      961 tcctccctac acctgggcaa taaatatgct tcatcttcag cttctaaact ccaagggtgg
     1021 aaagaagaaa aagtcctgag gaaccaacac aatttccagt cttctcacca gtctacacca
     1081 gtcacctgtc ttagcaaagg aatgccatat caccacctga tagtaccata aagagctctg
     1141 gatatctcct gtgtgagatg tagaaatcga cctagttttc agcgactttg ctaggaggaa
     1201 ctgtgatccc cccagcccag gcaggtgggt tcctggaaaa catgccgtag ttgagtttag
     1261 actttccagg ttttcttagc ctcgatccgt ggagaaactt gcctgttttc tttgagttct
     1321 tgtgttagcc agtgtcagta tttattgttt cccactgtgt taacacagga agagttttgc
     1381 aagtgtgtta attaatgtta aatgataact agaaatgttt cctttaataa agaatggctg
     1441 tactcagcat gctcaacatc aaaaaaaaaa aaaaaa
//
