LOCUS       NM_025318               1353 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ER membrane protein complex subunit 6 (Emc6),
            transcript variant 2, mRNA.
ACCESSION   NM_025318
VERSION     NM_025318.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Li,R., Wang,X., Zhang,X., Yu,J., Feng,J., Lv,P., Lou,Y. and Chen,Y.
  TITLE     Ad5-EMC6 mediates antitumor activity in gastric cancer cells
            through the mitochondrial apoptosis pathway
  JOURNAL   Biochem Biophys Res Commun 513 (3), 663-668 (2019)
   PUBMED   30982575
  REMARK    GeneRIF: Adenovirus-mediated ectopic overexpression of EMC6
            (Ad5-EMC6) decreases the activity of ERK1/2, down-regulates the
            levels of BCL-2 and increases the ratio of BAX/BCL-2. Ad5-EMC6
            mediates anti-tumor activity through the mitochondrial apoptosis
            pathway. Ad5-EMC6 enhances the sensitivity of gastric cancer cells
            to the etoposide.
REFERENCE   3  (bases 1 to 1353)
  AUTHORS   Guna,A., Volkmar,N., Christianson,J.C. and Hegde,R.S.
  TITLE     The ER membrane protein complex is a transmembrane domain insertase
  JOURNAL   Science 359 (6374), 470-473 (2018)
   PUBMED   29242231
REFERENCE   4  (bases 1 to 1353)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 1353)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL670399.4, AK004149.1 and AK003198.1.
            
            On Dec 16, 2009 this sequence version replaced NM_025318.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022104.1, AK003198.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AL670399.4         200899-200903       c
            6-498               AK004149.1         2-494
            499-1323            AK003198.1         494-1318
            1324-1353           AL670399.4         199364-199393       c
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.25 cM"
     gene            1..1353
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /note="ER membrane protein complex subunit 6"
                     /db_xref="GeneID:66048"
                     /db_xref="MGI:MGI:1913298"
     exon            1..104
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /inference="alignment:Splign:2.1.0"
     exon            105..1353
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..485
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /note="transmembrane protein 93"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 6"
                     /protein_id="NP_079594.1"
                     /db_xref="CCDS:CCDS24999.1"
                     /db_xref="GeneID:66048"
                     /db_xref="MGI:MGI:1913298"
                     /translation="MAAVVAKREGPPFISEAAVRGNAAVLDYCRTSVSALSGATAGIL
                     GLTGLYGFIFYLLASVLLSLLLILKAGRRWNKYFKSRRPLFTGGLIGGLFTYVLFWTF
                     LYGMVHVY"
     regulatory      643..648
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /note="hexamer: ATTAAA"
     polyA_site      660
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /note="major polyA site"
     regulatory      1308..1313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /note="hexamer: AATAAA"
     regulatory      1332..1337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /note="hexamer: AGTAAA"
     polyA_site      1337
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
                     /note="major polyA site"
     polyA_site      1353
                     /gene="Emc6"
                     /gene_synonym="0610009E20Rik; 0610025L18Rik; Tmem93"
ORIGIN      
        1 aaaccgagcc ctcccagcag cctccagcgc aagatggcgg cgcgggactc tccgcttttt
       61 cctgtctgag ggaacacgag ggactgtgtg ccgcggcgtc tcagctccgc gtgagtcggc
      121 gtgaaggcag aagcggagcc ccggcgggtg cgatggccgc ggtggtggcc aagcgggaag
      181 ggccgccgtt catcagcgag gcagccgtgc gaggcaacgc cgcggtcctg gattactgcc
      241 ggacctcagt gtcagcgctg tccggggcca ccgccggcat cctcggcctc accggcctct
      301 acggcttcat cttctacctg cttgcctccg tcctgctctc cctgctcctc attctcaaag
      361 cgggaaggag gtggaacaaa tattttaagt cacgaagacc tctctttacg ggaggcctca
      421 ttggaggcct cttcacctac gtcctctttt ggaccttcct ctatggcatg gtgcacgtct
      481 actgaaatga gggcaggggt gactttaaaa cagatcggca aggctcttgc cagaattaac
      541 accgggtaga cttacttaga ttttaaattg ttggaattcg ctgcttgttc tgtactgtta
      601 taatttatat ctgaagacag agcctgtaat attattcttc agattaaatg aagtgtgaca
      661 ccttgtcgtt ccttgcttag ccaagctttg aagtcgccaa gatttgctcc ctggagagca
      721 agtacgaggc cacagcctgt atattttttt ggatttaacg cctagtaagt ccaaagggac
      781 ctctagagta tacatgagag aatttaacgt taacatctta agaactggta acagttcgtg
      841 ttactaagct ttcactgtgt accggcattg tgtgttttca ttctcgccat agcaatggag
      901 atgttactgc taactttctg ttaatgaaaa acctgaagca cagagaagtt agttgtctct
      961 gccagaagtg gctcgtctgg aagggggcag ggccgaggtc cagttgcaag tctgtttgct
     1021 tttgctggat tgggtaagag tcctcttcta gcaccccctc ccactaacca ctgctttccc
     1081 acgtgagtga aatcagctaa gttccagtta caccaggttt agccagtcgc ctttcctata
     1141 acaaaaaatg caagctattt ttctaaatcg ttgtcctctg gcgattaggt tatgtggaca
     1201 tttaatcaaa tgagtttgtt tcagttgaaa tgcctctgac tatatgttca ttcataatcc
     1261 ttccttgaga ggagggtttt agaatgaaat acatgtttaa ctgaaaaaat aaatacttgt
     1321 gttagacttg tagtaaacat gttgcgcatg gaa
//
