LOCUS       NM_026519                941 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus ER membrane protein complex subunit 4 (Emc4),
            transcript variant 1, mRNA.
ACCESSION   NM_026519
VERSION     NM_026519.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 941)
  AUTHORS   Tang,X., Wei,W., Snowball,J.M., Nakayasu,E.S., Bell,S.M.,
            Ansong,C., Lin,X. and Whitsett,J.A.
  TITLE     EMC3 regulates mesenchymal cell survival via control of the mitotic
            spindle assembly
  JOURNAL   iScience 26 (1), 105667 (2022)
   PUBMED   36624844
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 941)
  AUTHORS   Guna,A., Volkmar,N., Christianson,J.C. and Hegde,R.S.
  TITLE     The ER membrane protein complex is a transmembrane domain insertase
  JOURNAL   Science 359 (6374), 470-473 (2018)
   PUBMED   29242231
REFERENCE   4  (bases 1 to 941)
  AUTHORS   Tang,X., Snowball,J.M., Xu,Y., Na,C.L., Weaver,T.E., Clair,G.,
            Kyle,J.E., Zink,E.M., Ansong,C., Wei,W., Huang,M., Lin,X. and
            Whitsett,J.A.
  TITLE     EMC3 coordinates surfactant protein and lipid homeostasis required
            for respiration
  JOURNAL   J Clin Invest 127 (12), 4314-4325 (2017)
   PUBMED   29083321
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL683897.5.
            
            On Mar 22, 2023 this sequence version replaced NM_026519.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK167745.1, BU571265.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AL683897.5         88115-88274         c
            161-275             AL683897.5         87698-87812         c
            276-429             AL683897.5         84448-84601         c
            430-590             AL683897.5         84088-84248         c
            591-941             AL683897.5         83300-83650         c
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 57.02 cM"
     gene            1..941
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /note="ER membrane protein complex subunit 4"
                     /db_xref="GeneID:68032"
                     /db_xref="MGI:MGI:1915282"
     exon            1..160
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..626
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /note="isoform a is encoded by transcript variant 1;
                     transmembrane protein 85"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 4 isoform a"
                     /protein_id="NP_080795.1"
                     /db_xref="CCDS:CCDS16553.1"
                     /db_xref="GeneID:68032"
                     /db_xref="MGI:MGI:1915282"
                     /translation="MTTQGGLVANRGRRFKWAIELSGPGGGSRGRSDRGSGQGDSLYP
                     VGYLDKQVPDTSVQETDRILVEKRCWDIALGPLKQIPMNLFIMYMAGNTISIFPTMMV
                     CMMAWRPIQALMAISATFKMLESSSQKFLQGLVYLIGNLMGLALAVYKCQSMGLLPTH
                     ASDWLAFIEPPERMEFSGGGLLL"
     exon            161..275
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /inference="alignment:Splign:2.1.0"
     exon            276..429
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /inference="alignment:Splign:2.1.0"
     exon            430..590
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /inference="alignment:Splign:2.1.0"
     exon            591..941
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /inference="alignment:Splign:2.1.0"
     regulatory      906..911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /note="hexamer: ATTAAA"
     polyA_site      941
                     /gene="Emc4"
                     /gene_synonym="2610318K02Rik; Tmem85"
                     /note="major polyA site"
ORIGIN      
        1 agttgcagct agttcttacc tgcagaacgc aagcggccgg agggtgtggg tacttttagc
       61 cagcggccgc tgtcatgacg acccaggggg gcctagtggc caaccgaggc cggcggttca
      121 aatgggccat tgagctgagt gggccgggag gaggcagcag gggccgaagt gaccggggca
      181 gtggacaggg agactccctc tatccagtcg gttacttgga caagcaagtg cctgatacca
      241 gcgtgcaaga gaccgaccgg atcctggtgg agaagcgctg ctgggacatc gccttgggtc
      301 ccctcaagca gattcccatg aacctcttca tcatgtacat ggcaggcaac accatttcca
      361 tcttccctac tatgatggtg tgtatgatgg cctggcgacc cattcaagca cttatggcca
      421 tttcagccac tttcaagatg ctggagagtt caagtcagaa gtttcttcaa ggcttggtct
      481 atctcattgg gaacctgatg ggtttggcat tggcagttta caagtgccag tccatgggac
      541 tgttgcctac acatgcatca gactggctag cctttattga gccccctgag agaatggagt
      601 tcagtggagg aggcttactt ctgtgacccc cagaaagcac catgcatatg gacctcatct
      661 gcattttaag ctgttgactc ctaaatatcc tcccttaaaa cagtcatgca tgtggagaag
      721 agcctcctgt ggtgaggcca gtgtgcagca tgttacagca gaaaagaaac tacagcacaa
      781 cttatgactg aaaataatgt agaaaacttt atttatgttc ccagcacaga gcaaaacaaa
      841 caaaaccaga ctctatgtaa acaaaagaat agcagctgct cagtagcagc ttctcctttc
      901 aataaattaa acggttgaga acaatgctta atgtttcaca a
//
