LOCUS       NM_025736               1209 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus ER membrane protein complex subunit 2 (Emc2),
            transcript variant 1, mRNA.
ACCESSION   NM_025736
VERSION     NM_025736.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Guna,A., Volkmar,N., Christianson,J.C. and Hegde,R.S.
  TITLE     The ER membrane protein complex is a transmembrane domain insertase
  JOURNAL   Science 359 (6374), 470-473 (2018)
   PUBMED   29242231
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   3  (bases 1 to 1209)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   4  (bases 1 to 1209)
  AUTHORS   Dreger,M., Bengtsson,L., Schoneberg,T., Otto,H. and Hucho,F.
  TITLE     Nuclear envelope proteomics: novel integral membrane proteins of
            the inner nuclear membrane
  JOURNAL   Proc Natl Acad Sci U S A 98 (21), 11943-11948 (2001)
   PUBMED   11593002
REFERENCE   5  (bases 1 to 1209)
  AUTHORS   Ehringer,M.A., Thompson,J., Conroy,O., Xu,Y., Yang,F., Canniff,J.,
            Beeson,M., Gordon,L., Bennett,B., Johnson,T.E. and Sikela,J.M.
  TITLE     High-throughput sequence identification of gene coding variants
            within alcohol-related QTLs
  JOURNAL   Mamm Genome 12 (8), 657-663 (2001)
   PUBMED   11471062
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099621.12 and AC099614.9.
            
            On Mar 18, 2023 this sequence version replaced NM_025736.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK014989.1, BC004716.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AC099621.12        4356-4420           c
            66-179              AC099614.9         37248-37361
            180-244             AC099614.9         39818-39882
            245-330             AC099614.9         40242-40327
            331-388             AC099614.9         42139-42196
            389-474             AC099614.9         52723-52808
            475-534             AC099614.9         52940-52999
            535-616             AC099614.9         55893-55974
            617-727             AC099614.9         56778-56888
            728-832             AC099614.9         58823-58927
            833-1209            AC099614.9         72475-72851
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 16.75 cM"
     gene            1..1209
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /note="ER membrane protein complex subunit 2"
                     /db_xref="GeneID:66736"
                     /db_xref="MGI:MGI:1913986"
     exon            1..65
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..919
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tetratricopeptide repeat protein 35; TPR repeat protein
                     35; tetratricopeptide repeat domain 35"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 2 isoform 1"
                     /protein_id="NP_080012.1"
                     /db_xref="CCDS:CCDS27453.1"
                     /db_xref="GeneID:66736"
                     /db_xref="MGI:MGI:1913986"
                     /translation="MAKVTERYDVTWEEMRDKMRKWREENSRNSEQIMEVGEELINDY
                     ASKLGDDIWIIYEQVMIAALDYGRDDLALFCLQELRRQFPGSHRVKRLTGMRFEAMER
                     YDDAIQLYDRILQEDPTNTAARKRKIAIRKAQGKTVEAIRELNEYLEQFVGDQEAWHE
                     LAELYINEHDYAKAAFCLEELMMTNPHNHLYCQQYAEVKYTQGGLENLELSRKYFAQA
                     LKLNNRNMRALFGLYMSASHIASNPKASAKMKKDNIKYASWAANQINRAYQFAGRSKK
                     ETKYSLKAVEDMLETLQITQS"
     exon            66..179
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            180..244
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            245..330
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            331..388
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            389..474
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            475..534
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            535..616
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            617..727
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            728..832
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     exon            833..1209
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1175..1180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /note="hexamer: AATAAA"
     regulatory      1190..1195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /note="hexamer: ATTAAA"
     polyA_site      1199
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /note="major polyA site"
     polyA_site      1209
                     /gene="Emc2"
                     /gene_synonym="4921531G14Rik; Ttc35"
                     /note="major polyA site"
ORIGIN      
        1 ctcgccgact ccgggttcta ggaagatggc gaaggtcact gagcgttacg atgtgacttg
       61 ggaagaaatg cgagataaaa tgagaaaatg gcgagaagaa aactcaagaa atagtgaaca
      121 aattatggaa gttggagaag aattaattaa tgattatgct tctaagcttg gggatgacat
      181 ttggatcata tatgagcagg tcatgattgc agccctagac tatggtcggg atgacttggc
      241 attgttttgt cttcaggaat taagaagaca attccctggt agtcacagag ttaagcggtt
      301 aacaggcatg cgatttgaag ctatggaaag atatgacgat gctatacaac tgtatgatcg
      361 gattttgcaa gaagatccaa ctaacactgc tgccagaaag cgtaagattg ccattcgaaa
      421 agcccagggg aaaaccgtgg aggccatccg agagctgaat gagtatctgg agcagtttgt
      481 tggagaccaa gaagcctggc atgaacttgc agaactttat atcaatgaac acgactatgc
      541 caaagcagct ttttgcttag aggagctgat gatgacaaat ccacataacc acttatactg
      601 tcagcagtat gcagaagtca aatacaccca aggtggactt gaaaacctcg agctttcaag
      661 aaagtatttt gcacaggcac tgaaactcaa caacaggaac atgagagctc tgtttgggct
      721 ctacatgtct gcaagtcata ttgcttctaa tccaaaagca agtgcaaaaa tgaaaaaaga
      781 caacatcaaa tatgctagtt gggccgctaa tcaaataaac agggcttatc agtttgcagg
      841 tcgaagtaag aaggaaacca aatactctct taaggcagtt gaagacatgt tggagacatt
      901 gcagatcact cagtcttgag gtttcaggaa cccatttgac gttagatttc aaactacaca
      961 gttgaacttt ttggcctgtg actgaattta ctaaatatct agtgctattt gtattctaat
     1021 tttctctgaa gaggttgtta taaagaatgt ttaactaaac ctcagcttgc cttgtactgc
     1081 taggaagcag ggagatggag gtaatccatg ggacagagat ttaagaccta ttgattgtat
     1141 atccatcctc gtatatcacc cctacacctt attaaataaa ccatggttta ttaaactcat
     1201 gtatgaaaa
//
