LOCUS       NM_197991               1761 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ER membrane protein complex subunit 10 (Emc10),
            transcript variant 1, mRNA.
ACCESSION   NM_197991 XM_133467
VERSION     NM_197991.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Wang,X., Li,Y., Qiang,G., Wang,K., Dai,J., McCann,M., Munoz,M.D.,
            Gil,V., Yu,Y., Li,S., Yang,Z., Xu,S., Cordoba-Chacon,J., De
            Jesus,D.F., Sun,B., Chen,K., Wang,Y., Liu,X., Miao,Q., Zhou,L.,
            Hu,R., Ding,Q., Kulkarni,R.N., Gao,D., Bluher,M. and Liew,C.W.
  TITLE     Secreted EMC10 is upregulated in human obesity and its neutralizing
            antibody prevents diet-induced obesity in mice
  JOURNAL   Nat Commun 13 (1), 7323 (2022)
   PUBMED   36443308
  REMARK    GeneRIF: Secreted EMC10 is upregulated in human obesity and its
            neutralizing antibody prevents diet-induced obesity in mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1761)
  AUTHORS   Liu,L., Mao,S., Chen,K., Dai,J., Jin,S., Chen,L., Wang,Y., Guo,L.,
            Yang,Y., Zhan,C., Xiong,Z., Diao,H., Zhou,Y., Ding,Q. and Wang,X.
  TITLE     Membrane-Bound EMC10 Is Required for Sperm Motility via Maintaining
            the Homeostasis of Cytoplasm Sodium in Sperm
  JOURNAL   Int J Mol Sci 23 (17), 10069 (2022)
   PUBMED   36077468
  REMARK    GeneRIF: Membrane-Bound EMC10 Is Required for Sperm Motility via
            Maintaining the Homeostasis of Cytoplasm Sodium in Sperm.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1761)
  AUTHORS   Zhou,Y., Wu,F., Zhang,M., Xiong,Z., Yin,Q., Ru,Y., Shi,H., Li,J.,
            Mao,S., Li,Y., Cao,X., Hu,R., Liew,C.W., Ding,Q., Wang,X. and
            Zhang,Y.
  TITLE     EMC10 governs male fertility via maintaining sperm ion balance
  JOURNAL   J Mol Cell Biol 10 (6), 503-514 (2018)
   PUBMED   29659949
  REMARK    GeneRIF: Male mice lacking Emc10 gene are completely sterile.
            Emc10-null spermatozoa exhibit multiple defects including abnormal
            morphology, decreased motility, impaired capacitation, and
            impotency of acrosome reaction, thereby which are incapable of
            fertilizing intact or ZP-free oocytes.
REFERENCE   5  (bases 1 to 1761)
  AUTHORS   Guna,A., Volkmar,N., Christianson,J.C. and Hegde,R.S.
  TITLE     The ER membrane protein complex is a transmembrane domain insertase
  JOURNAL   Science 359 (6374), 470-473 (2018)
   PUBMED   29242231
REFERENCE   6  (bases 1 to 1761)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   7  (bases 1 to 1761)
  AUTHORS   Probert,F., Rice,P., Scudamore,C.L., Wells,S., Williams,R.,
            Hough,T.A. and Cox,I.J.
  TITLE     (1)H NMR metabolic profiling of plasma reveals additional
            phenotypes in knockout mouse models
  JOURNAL   J Proteome Res 14 (5), 2036-2045 (2015)
   PUBMED   25849460
REFERENCE   8  (bases 1 to 1761)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (bases 1 to 1761)
  AUTHORS   Xu,B., Hsu,P.K., Stark,K.L., Karayiorgou,M. and Gogos,J.A.
  TITLE     Derepression of a neuronal inhibitor due to miRNA dysregulation in
            a schizophrenia-related microdeletion
  JOURNAL   Cell 152 (1-2), 262-275 (2013)
   PUBMED   23332760
REFERENCE   10 (bases 1 to 1761)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149607.6.
            
            On Apr 10, 2019 this sequence version replaced NM_197991.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150499.1, AK169010.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-163               AC149607.6         54080-54242
            164-236             AC149607.6         55618-55690
            237-346             AC149607.6         57199-57308
            347-451             AC149607.6         57840-57944
            452-633             AC149607.6         58173-58354
            634-727             AC149607.6         58450-58543
            728-1761            AC149607.6         59528-60561
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.83 cM"
     gene            1..1761
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /note="ER membrane protein complex subunit 10"
                     /db_xref="GeneID:69683"
                     /db_xref="MGI:MGI:1916933"
     exon            1..163
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..826
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; UPF0510 protein INM02; hematopoietic signal
                     peptide-containing membrane domain-containing protein 1"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 10 isoform 1
                     precursor"
                     /protein_id="NP_932108.3"
                     /db_xref="CCDS:CCDS52231.2"
                     /db_xref="GeneID:69683"
                     /db_xref="MGI:MGI:1916933"
                     /translation="MVAAGAGVTRLLVLLLMVAAAPSRARGSGCRVGASARGTGADGR
                     EAEGCGTVALLLEHSFELGDGANFQKRGLLLWNQQDGTLSATQRQLSEEERGRLRDVA
                     AVNGLYRVRVPRRPGTLDGSEAGGHVSSFVPACSLVESHLSDQLTLHVDVAGNVVGLS
                     VVVYPGGCRGSEVEDEDLELFNTSVQLRPPSTAPGPETAAFIERLEMEQAQKAKNPQE
                     QKSFFAKYWMYIIPVVLFLMMSGAPDAGGQGGGGGGGSSR"
     exon            164..236
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /inference="alignment:Splign:2.1.0"
     exon            237..346
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /inference="alignment:Splign:2.1.0"
     exon            347..451
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /inference="alignment:Splign:2.1.0"
     exon            452..633
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /inference="alignment:Splign:2.1.0"
     exon            634..727
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /inference="alignment:Splign:2.1.0"
     exon            728..1761
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1552..1557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /note="hexamer: ATTAAA"
     polyA_site      1575
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /note="major polyA site"
     regulatory      1695..1700
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
                     /note="hexamer: ATTAAA"
     polyA_site      1728
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
     polyA_site      1761
                     /gene="Emc10"
                     /gene_synonym="2310044H10Rik; 5430410O10Rik; Inm02;
                     Mirta22"
ORIGIN      
        1 gtgtttccta gcgggctgta tggctctcgg tttttctcaa cgctcccgta tggtggccgc
       61 gggtgccggg gtgacccggc tgctagtgct cttgctgatg gtagccgcgg ctcctagcag
      121 agcccgaggc agcggctgcc gggtcggggc ctccgcgcgt gggaccgggg ccgatggccg
      181 tgaagctgag ggctgtggca ccgtggcttt gctgctggag cattcatttg agctcggtga
      241 tggagccaac ttccagaagc gaggcttgct gctctggaac cagcaggatg gcaccctgtc
      301 ggcaacacag cgacagctca gtgaggagga gcgtggccga ctccgggatg tggctgctgt
      361 caatggcctc tacagggtcc gggtcccgag gcggcctggg acacttgatg gttcagaagc
      421 tggcggccat gtgtcttcct tcgtcccagc gtgctccctg gtggagtcgc acctttcgga
      481 ccagctgacc ttgcacgtgg atgtggctgg caacgtggtg ggcctgtctg tggtggtgta
      541 ccctgggggc tgccggggct ccgaggtgga agatgaggac ctggagctgt tcaatacatc
      601 tgtgcagctg cggcctccca gcactgctcc aggccccgag actgcagcct tcattgagcg
      661 cctggagatg gagcaggccc agaaggccaa gaacccacag gagcagaagt ctttctttgc
      721 caaatactgg atgtacatca ttccagttgt gctgttcctc atgatgtcgg gagcgccgga
      781 cgctgggggc cagggcggcg gtgggggcgg gggcagcagc cggtgagcag ctgtgccacc
      841 tagagccccc cccagagcca gcccaagaag gagttcctgt ccccacattt ccctattgca
      901 tgaatatgga aggctgtccc ttcagtgagc cctctggcct tcctgtaagc ccctctttct
      961 gtccctgagc ctctctctca tcctgttgac tgagagcttg ggtggacctc cctgtagcca
     1021 gctcactgca actgtgtccc accatgtggc actgtgctcc tctgtctgct aaacacccac
     1081 cagcctgccc caccccaccc caccatacac tttgggaact tgccaagctc tctccagcct
     1141 ctgtgccttt gccctgcagg ccccgtgcgc ccctcactgt cactctccag ccctttgcca
     1201 aggatctgtg gcccagaggc ctctgctctt agtggctagg tcagcctcca gcccactgtc
     1261 caggtggcat gctgtcttct ttgcccccct ctctggtgcc ccagaatacc atggtgacct
     1321 accactatcc tttctgcctt tggatgtcat agcctggatc tgtcaccagg agaggattgt
     1381 gggcctccac gttagtctgt gaatgcacac ttcgagtgac ttgtgtgcag gttttgagag
     1441 ccggttttgc actagctgct cgacagctgc tggcatggcc gtgctcttgc acatgcgccg
     1501 ctgtgggcat ggggattgct gtgcagcctc agctgtgttg tgtggctgct gattaaactg
     1561 tcccctaaac agccactctt cagctcactt cctgccttct gtgcttgtga atagtcctga
     1621 gttgccgctg tggtttgcct ggtttatgtt tgaatggctt tcttagggta tgttacagag
     1681 gggtgcctga gcagattaaa gttgctgtga gcaaggacgc cttccgaact ctgggaggag
     1741 gctggttcct gaccctccta a
//
