LOCUS       NM_146157               6237 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus ER membrane protein complex subunit 1 (Emc1),
            transcript variant 1, mRNA.
ACCESSION   NM_146157
VERSION     NM_146157.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Li,X., Jiang,Z., Su,Y., Wang,K., Jiang,X., Sun,K., Yang,Y.,
            Zhou,Y., Zhu,X. and Zhang,L.
  TITLE     Deletion of Emc1 in photoreceptor cells causes retinal degeneration
            in mice
  JOURNAL   FEBS J 290 (17), 4356-4370 (2023)
   PUBMED   37098815
  REMARK    GeneRIF: Deletion of Emc1 in photoreceptor cells causes retinal
            degeneration in mice.
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Guna,A., Volkmar,N., Christianson,J.C. and Hegde,R.S.
  TITLE     The ER membrane protein complex is a transmembrane domain insertase
  JOURNAL   Science 359 (6374), 470-473 (2018)
   PUBMED   29242231
REFERENCE   3  (bases 1 to 6237)
  AUTHORS   Tang,X., Snowball,J.M., Xu,Y., Na,C.L., Weaver,T.E., Clair,G.,
            Kyle,J.E., Zink,E.M., Ansong,C., Wei,W., Huang,M., Lin,X. and
            Whitsett,J.A.
  TITLE     EMC3 coordinates surfactant protein and lipid homeostasis required
            for respiration
  JOURNAL   J Clin Invest 127 (12), 4314-4325 (2017)
   PUBMED   29083321
REFERENCE   4  (bases 1 to 6237)
  AUTHORS   Fu,B., Wang,L., Li,S. and Dorf,M.E.
  TITLE     ZMPSTE24 defends against influenza and other pathogenic viruses
  JOURNAL   J Exp Med 214 (4), 919-929 (2017)
   PUBMED   28246125
REFERENCE   5  (bases 1 to 6237)
  AUTHORS   Yamamoto,S., Yamazaki,T., Komazaki,S., Yamashita,T., Osaki,M.,
            Matsubayashi,M., Kidoya,H., Takakura,N., Yamazaki,D. and
            Kakizawa,S.
  TITLE     Contribution of calumin to embryogenesis through participation in
            the endoplasmic reticulum-associated degradation activity
  JOURNAL   Dev Biol 393 (1), 33-43 (2014)
   PUBMED   25009997
REFERENCE   6  (bases 1 to 6237)
  AUTHORS   Nakano,Y., Jahan,I., Bonde,G., Sun,X., Hildebrand,M.S.,
            Engelhardt,J.F., Smith,R.J., Cornell,R.A., Fritzsch,B. and Banfi,B.
  TITLE     A mutation in the Srrm4 gene causes alternative splicing defects
            and deafness in the Bronx waltzer mouse
  JOURNAL   PLoS Genet 8 (10), e1002966 (2012)
   PUBMED   23055939
REFERENCE   7  (bases 1 to 6237)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY281473.1, AK049061.1 and AL807833.5.
            
            On Sep 25, 2013 this sequence version replaced NM_146157.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049061.1, AK077496.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BY281473.1         2-12
            12-3234             AK049061.1         3-3225
            3235-6237           AL807833.5         3134-6136
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 70.66 cM"
     gene            1..6237
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /note="ER membrane protein complex subunit 1"
                     /db_xref="GeneID:230866"
                     /db_xref="MGI:MGI:2443696"
     exon            1..131
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..3027
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; novel DUF1620 domain containing protein"
                     /codon_start=1
                     /product="ER membrane protein complex subunit 1 isoform 1
                     precursor"
                     /protein_id="NP_666269.2"
                     /db_xref="CCDS:CCDS38933.1"
                     /db_xref="GeneID:230866"
                     /db_xref="MGI:MGI:2443696"
                     /translation="MAVAVASGFWIWAAVLLVPAAAVYEDQVGKFDWRQQYVGKIKFA
                     SLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLVHGQDAITVS
                     NGGRLMRSWETNIGGLNWEITLDTGSFQALGLVGLQESVRYIAVLKKTTLTLHHLSSG
                     HLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPFSHVNIVKFNVEDGEIVQQVRVWT
                     PWLQHLTGACGVVDEAVLVCPDPSSHSLHTLALETEWELRQIPLQSPDLEFGSGFQPQ
                     VLPTQPSPVAPSRAQFFLQLSPSHYALLHYHHGAVTLLKNFPQATLVSFATTGEKTVA
                     AVMTCRTEVQKPVSAGDGSVASFPETSGAQDSLACFNQTYTINLYLVETGRRLLDTSI
                     SFSLEQKGTRPEQLYIQVFLKKDDSVGYRALVQTQDHLQLFLQQLAGKVVLWSREESL
                     AEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWK
                     MFYDARKPRSQIKNEINIDTLARDEFNLQKMMVTVTASGKLFGIESSSGTILWKQYLP
                     NVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKETGMSSLFVFNPIFGKWSQVAPPVLK
                     RPILQSLLLPVMDQDYAKVLLLVDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQ
                     GRLSGYQLRKDLTTELSWELTIPPEVQRVVKVKGKRSSEHVHSQGRVMGDRSVLYKSL
                     NPNLLAVVTESTDVHHERTFIGIFLIDGVTGRIIHSSVQKKARGPVHLVHSENWVVYQ
                     YWNSKARRNELTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATIT
                     ERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQVHAERFI
                     NYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSV
                     LFGLVFATMITKRLAQVKLLNRAWR"
     exon            132..256
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            257..322
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            323..416
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            417..545
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            546..672
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            673..822
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            823..990
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1062
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1125
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1248
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1345
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1468
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1477
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1677
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1827
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1989
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2109
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2247
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2421
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2632
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2717
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2847
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..6237
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3429
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /note="major polyA site"
     regulatory      4936..4941
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4955
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /note="major polyA site"
     regulatory      6194..6199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      6215
                     /gene="Emc1"
                     /gene_synonym="2700016F22Rik"
ORIGIN      
        1 gggctggcgg caggcggtgc ttgggcttca atcatggcgg tcgcggtggc gtctgggttc
       61 tggatctggg ctgctgtgct gctcgtccct gcggccgcgg tctacgaaga ccaagtgggc
      121 aagtttgact ggagacagca gtatgttggg aagatcaagt ttgcctcctt ggagttttcc
      181 cctggatcca agaagttggt tgtggccaca gagaagaatg tgattgcggc gttaaattct
      241 cggactggag agatcttgtg gcgccatgtt gacaaaggca cagcagaagg agctgtggat
      301 gccatgctgg tccatggaca agatgcaatc actgtatcca acggagggcg cctcatgcgt
      361 tcctgggaga ccaacatcgg gggcctgaac tgggagataa ctctggacac tggcagtttc
      421 caggcacttg ggctagtggg ccttcaggag tcagtgaggt acattgcagt tctgaagaag
      481 acgaccctca ccctccacca cctctccagt gggcacctga agtgggtgga gcatctgcca
      541 gaaagtgaca gcatcctcta ccagatggtg tattcctacg gctccggggt ggtgtgggcc
      601 cttggcatcg ttcccttcag ccacgtgaac atcgtcaagt ttaacgtgga ggatggagag
      661 attgttcagc aggtcagggt ttggactcca tggctgcagc acctcactgg ggcctgtggt
      721 gtggtagatg aggctgtcct ggtatgcccc gaccccagct cacactcact ccacactttg
      781 gccctggaga cggagtggga actgcgacag atccccctgc agtctcctga cttagaattt
      841 ggaagtggat tccagcccca agtgctgccc acacaaccca gcccagtggc tccttctcga
      901 gcccagttct tcctgcagtt gtccccaagc cactacgctc tactgcacta ccaccatggt
      961 gccgtgactc tgcttaagaa cttcccccag gccacgctcg tcagcttcgc caccacaggg
     1021 gagaagacag tggctgcagt catgacctgt cgcactgaag tgcaaaaacc tgtcagtgct
     1081 ggagatgggt ctgtggcaag ctttcctgag acatctggtg cacaggactc tctggcttgc
     1141 ttcaaccaga cctacaccat taacctgtac ttagtggaga caggtcggcg gctcctagac
     1201 acttccattt ccttcagcct ggagcagaag ggcacgcgac ctgagcagct gtacatccag
     1261 gtgttcttga agaaggacga ctcggtgggc taccgggcct tggtgcagac acaggaccat
     1321 ctgcagctgt tcctgcagca gcttgctggg aaggtggtgt tgtggagccg agaggagtcc
     1381 ctggcagaag tcgtgtgcct ggagatggtg gatctccccc tgactggagc gcaggctgag
     1441 ttggaaggag aatttggcaa gaaggcagcg attcaagatg gcttgctggg gatgttcctg
     1501 aaacgcctgt cgtcccagct catcctgctc caggcctgga cttcccacct ctggaaaatg
     1561 ttctacgatg ctcggaagcc ccgaagccag attaagaatg agatcaacat cgacacactg
     1621 gctagagatg agttcaacct gcagaaaatg atggtgacgg taacagcctc aggcaagctc
     1681 tttggcatcg agagcagctc tggaaccatt ctgtggaagc agtatctgcc caatgttaag
     1741 ccagactcct cctttaagct gatggtgcag aggactactg cccatttccc ccatccaccc
     1801 cagtgcacgc tgctggtgaa ggacaaggag acaggaatga gctctctgtt tgtcttcaac
     1861 cccatttttg gcaagtggag ccaggtagct cccccagtgc tgaagcgccc catcttgcag
     1921 tcactgcttc tcccagtcat ggatcaagac tatgccaagg tgttgctgtt ggtggatgac
     1981 gaatacaagg tcacagcttt cccagctacg cggaatgtct tgcggcagct gcatgaactt
     2041 gccccctcca tcttcttcta cttggtggat gcagagcagg ggcggctcag tgggtatcag
     2101 cttcgaaagg atctcactac agaactgagt tgggagctga ccatcccccc agaagtgcag
     2161 cgcgtcgtca aggtcaaggg gaagcgcagc agcgagcacg tacactccca gggccgggtg
     2221 atgggggatc gaagtgtgct ctacaagagt ctgaacccca acttgctggc cgtggtgaca
     2281 gagagcacgg atgtccacca cgagcgcacc tttattggca tcttcctcat tgatggtgtc
     2341 actggccgca tcatccactc ctccgtgcag aagaaggcca ggggccctgt ccaccttgtg
     2401 cattcagaga actgggtggt gtaccagtac tggaattcta aggctcggcg caatgagctg
     2461 acggccctgg agctctacga gggcactgaa cagtacaatg ccactgcctt cagctctcta
     2521 gaccgacccc agctgcccca ggtcctccag cagtcctaca tcttcccctc ctccatcagt
     2581 gccatggaag ctaccatcac cgagaggggc atcaccagcc ggcacttgct cattgggctg
     2641 ccttctggag caatcctttc cctccccaag gccttgctgg atccccggcg cccagagatc
     2701 ccaacagagc aaagcagaga agagaacctg atcccatatt ctccagatgt tcaggtccat
     2761 gcagagcgat tcatcaacta taaccagaca gtctctcgaa tgcgaggcat ctatacagcg
     2821 ccctcaggcc tggagtccac ttgtttggtt gtggcctacg gtttggatat ttaccaaact
     2881 cgagtttacc cgtccaagca gtttgatgtc ctgaaagatg actacgacta tgtgctcatc
     2941 agcagtgtcc tttttggcct ggtttttgcc accatgatca caaagaggct ggcacaggtg
     3001 aaactcctca atcgagcctg gcggtaaaat gggagcaccg tgccagagtg gagcctcggg
     3061 aagagggaca gctaaggaga tggccgtgga ctgttggatc cctgaggttg ggctgggctc
     3121 ttgtctcact tacgtctggg agaagaaggt gcaccttcat ggagaagtgc ttcgggaaca
     3181 cggtgacgaa cagttcttgt ggtcagatac ccacctcagc gtggaggagc tccgtgcatc
     3241 tgatccactc cctgttccct ttatgtgctc attcgtggat gctgtgagcc ttttgttttt
     3301 gttttctttt tgtttttttg ttttttttcc tttcatgaga cctctggttt ctttcttctg
     3361 agactgttta aattgacctt agttaacctt ctgtatttcc agtcttacta gaaatattct
     3421 gacaccttat tctcttgtct cttgctttca agcttgatgg gccagctctg gctttaaggt
     3481 gtgtgtggct gcctgactga tggggtggat gcaggagcca ttcttagcca aggtctgagg
     3541 acttctctct ctgaagagaa gttgaaggat ccatacatgg agtgagaact aattttaacc
     3601 aagctgtcat tccatgccac catttatatg gctgggccat ttctttcagc ctatcaaagc
     3661 caagcacata acagtaattt ggtttttcat ccacaacctg tgctttttct cctgagctat
     3721 agcaaggcta aaatctttaa tacttttctt tcttttttct tttttttgtt tttgtagaca
     3781 gggtttctct gtgtaactct gactgtccta gaactctctt tgtagaccag gttggtcttg
     3841 aactcacaga gatcttcctg cctctgcctc ccaagtgctg ggatctaagg tgtgtgcctt
     3901 agggctaaag ccttttaaaa ctaacagtct gagccttggt gagatcttga ggtccagtac
     3961 atcaaatcta ttgtccctat aggcccctat agacatcctg tagcacagct ggaggaaagc
     4021 agctttgtta aaaccttttt tttttttttt ggcttttcga gacagagttt ctctgtatag
     4081 ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca gaaatccgcc
     4141 tgcctctgtc tcccaagtgc tgggattaaa aggcatgcat caccatgctt agcttttttt
     4201 ttttttttta aagacagagt tttgctcagt tagcccaggc tggcctcaaa cttgcaatcc
     4261 tcctgcctta gcctcccagt gttgggattg ctggtgttgg ccatcatgct tggtggtggg
     4321 caagttttaa atctcattgg tataattttc tgcgtggcat taggggtaga atccaaggtc
     4381 tctcatgcca ggccagttct ctgcactgaa gctctccata aggtgagcca cgagggctgt
     4441 tgtttttctt agttttaaaa tcttaataat ttttttgagg ggctgcaagg aggatgctgt
     4501 gcctcaaacc cagggcctca tgtgtgttag gacacacttt actactactg agctagagcc
     4561 tcaacacatc taatcttttt acttatttta ttgagacagg atctcaccat gtaaccatgg
     4621 ctgggctaga actagctatg tagtctaggg ttgggcttct gtgtgtggtg tgtatgcact
     4681 actgtttggg ctccttctgt ggtgtgtgta tgcactcctg tataggcttc tgtgtggtgt
     4741 gtgtgcattc ctgtctgagt ttctgtgtgg ggtgtgtgtg tgttttcctg tttgggcttc
     4801 tgtgttaggt gtgtgtactc ctgtctgggc ttctgtatgc tgcacagaaa gaatcccaag
     4861 tttgaccttg cttatgaact tggggactgc ttgttctttt gagcagggaa gtcaaagagt
     4921 tgaccacgct cctggaataa agctgatgat cggtggctca gctttgtttt gtgtcttcag
     4981 agttcacttc aaggctgctt ttgatggggg ggtggggaag aaaagccaca catggaggca
     5041 tacccctata atagcagcca cttgtggagg cacaccccta taatagcagc gcgtgagagg
     5101 tggagcaggg agagtcagaa gttgaaggtc atcttcagct acatattcag cctatgctct
     5161 aagagacagt ggttagaagt acctgttgct ttttcagctc cgtccctagt acccacattg
     5221 ggtgcctcac aagcacctgt atccccacct ccaggagatc tagcatcctt gcctggcctc
     5281 ggggtgtgca catatgcaca cacatagtaa cttagaaaac aaacaggaaa aaatggagtt
     5341 atgtagggca aaataccaac atcttcatca gtaataacca gtttcctctc ctgggttagt
     5401 gagtatctga cacttccagt gtcagtccct gcctggactg ctggcttcgt ggaaagccac
     5461 ctgggagaag gcattggaac ccattacact tttctgctct agtttttgtt ttgttataag
     5521 ctctcacctt gtgaatctgg gagatgtgct tggattaaag gcatatatca ctgtacccca
     5581 cctgctctag tcttgaaata gagcagctgg aattttgtgc aagatagata agtaactctt
     5641 accacactgc cctgtttgtt tgtcttggtt ttggttgctt taagatttat gagtgtctcc
     5701 cctgcatctg cgtgcatgcc tggtgcccac ggaggccaga agatgctgga tcccttggaa
     5761 ctagatttag ggacaattgt gaccatgtag gtcctgggaa ctgaactgag ccctctggag
     5821 gaactgaatg atgtgtctct tatagtgcct gctgtgtttc tacagtacag ttagtctgca
     5881 agactgtttt ttcacttacc ggccagaaaa agtggctaat ggtcgggaag tgacctacct
     5941 tttctagaac tcccggtatt tcacctttaa cctttaccat atcaccaaag ctgctcagag
     6001 cagctgactg aaatagccaa ggcctgagtg aagtcttaac ttgtctccta agtctttatt
     6061 ttcctttatt attattatta ttatttatat attgtctgtc tctatagctc tgactttcct
     6121 ggaactccct gtatagacca agctggcctt gcattcacaa agctctgcct gcctctgcct
     6181 tctgagcctt gagattaaaa aagtgtgggc caccatgcct ggcttcatat tattttt
//
