LOCUS       NM_026079               6160 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus elongator complex protein 1 (Elp1), mRNA.
ACCESSION   NM_026079
VERSION     NM_026079.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Arnskotter,F., da Silva,P.B.G., Schouw,M.E., Lukasch,C.,
            Bianchini,L., Sieber,L., Garcia-Lopez,J., Ahmad,S.T., Li,Y.,
            Lin,H., Joshi,P., Spanig,L., Rados,M., Roiuk,M., Sepp,M.,
            Zuckermann,M., Northcott,P.A., Patrizi,A. and Kutscher,L.M.
  TITLE     Loss of Elp1 in cerebellar granule cell progenitors models ataxia
            phenotype of Familial Dysautonomia
  JOURNAL   Neurobiol Dis 199, 106600 (2024)
   PUBMED   38996985
REFERENCE   3  (bases 1 to 6160)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 6160)
  AUTHORS   Harripaul,R., Morini,E., Salani,M., Logan,E., Kirchner,E.,
            Bolduc,J., Chekuri,A., Currall,B., Yadav,R., Erdin,S.,
            Talkowski,M.E., Gao,D. and Slaugenhaupt,S.
  TITLE     Transcriptome analysis in a humanized mouse model of familial
            dysautonomia reveals tissue-specific gene expression disruption in
            the peripheral nervous system
  JOURNAL   Sci Rep 14 (1), 570 (2024)
   PUBMED   38177237
  REMARK    Erratum:[Sci Rep. 2024 May 2;14(1):10103. doi:
            10.1038/s41598-024-60576-8.. PMID: 38698036]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6160)
  AUTHORS   Schultz,A., Cheng,S.Y., Kirchner,E., Costello,S., Miettinen,H.,
            Chaverra,M., King,C., George,L., Zhao,X., Narasimhan,J.,
            Weetall,M., Slaugenhaupt,S., Morini,E., Punzo,C. and Lefcort,F.
  TITLE     Reduction of retinal ganglion cell death in mouse models of
            familial dysautonomia using AAV-mediated gene therapy and splicing
            modulators
  JOURNAL   Sci Rep 13 (1), 18600 (2023)
   PUBMED   37903840
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6160)
  AUTHORS   Johansen,L.D., Naumanen,T., Knudsen,A., Westerlund,N., Gromova,I.,
            Junttila,M., Nielsen,C., Bottzauw,T., Tolkovsky,A., Westermarck,J.,
            Coffey,E.T., Jaattela,M. and Kallunki,T.
  TITLE     IKAP localizes to membrane ruffles with filamin A and regulates
            actin cytoskeleton organization and cell migration
  JOURNAL   J Cell Sci 121 (Pt 6), 854-864 (2008)
   PUBMED   18303054
REFERENCE   7  (bases 1 to 6160)
  AUTHORS   Hims,M.M., Shetty,R.S., Pickel,J., Mull,J., Leyne,M., Liu,L.,
            Gusella,J.F. and Slaugenhaupt,S.A.
  TITLE     A humanized IKBKAP transgenic mouse models a tissue-specific human
            splicing defect
  JOURNAL   Genomics 90 (3), 389-396 (2007)
   PUBMED   17644305
  REMARK    GeneRIF: description of a humanized IKBKAP transgenic mouse that
            models a tissue-specific human splicing defect
REFERENCE   8  (bases 1 to 6160)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 6160)
  AUTHORS   Coli,R., Anderson,S.L., Volpi,S.A. and Rubin,B.Y.
  TITLE     Genomic organization and chromosomal localization of the mouse
            IKBKAP gene
  JOURNAL   Gene 279 (1), 81-89 (2001)
   PUBMED   11722848
REFERENCE   10 (bases 1 to 6160)
  AUTHORS   Cuajungco,M.P., Leyne,M., Mull,J., Gill,S.P., Gusella,J.F. and
            Slaugenhaupt,S.A.
  TITLE     Cloning, characterization, and genomic structure of the mouse
            Ikbkap gene
  JOURNAL   DNA Cell Biol 20 (9), 579-586 (2001)
   PUBMED   11747609
  REMARK    GeneRIF: mutations in the human I-kappaB kinase-associated protein
            (IKBKAP) gene are responsible for familial dysautonomia (FD).no
            animal model of FD currently exists, cloning of the mouse Ikbkap
            gene is an important step toward creating a mouse model for FD
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK035944.1, AK147153.1, AK165168.1, AK032563.1 and AK076152.1.
            
            On Oct 31, 2007 this sequence version replaced NM_026079.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF387811.1, AF140786.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3081              AK035944.1         3-3083
            3082-4836           AK147153.1         3085-4839
            4837-5064           AK165168.1         4315-4542
            5065-6105           AK032563.1         1234-2274
            6106-6160           AK076152.1         2807-2861
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 31.66 cM"
     gene            1..6160
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="elongator complex protein 1"
                     /db_xref="GeneID:230233"
                     /db_xref="MGI:MGI:1914544"
     exon            1..173
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            174..336
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..4188
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="IKK-complex-associated protein IKAP; inhibitor of
                     kappa light polypeptide enhancer in B cells, kinase
                     complex-associated protein; ikappaB kinase
                     complex-associated protein; IKK complex-associated
                     protein"
                     /codon_start=1
                     /product="elongator complex protein 1"
                     /protein_id="NP_080355.2"
                     /db_xref="CCDS:CCDS38765.1"
                     /db_xref="GeneID:230233"
                     /db_xref="MGI:MGI:1914544"
                     /translation="MRNLKLHRTLEFRDIQAPGKPQCFCLRAEQGTVLIGSERGLTEV
                     DPVRREVKTEISLVAEGFLPEDGSGCIVGIQDLLDQESVCVATASGDVIVCNLSTQQL
                     ECVGSVASGISVMSWSPDQELLLLATAQQTLIMMTKDFEVIAEEQIHQDDFGEGKFVT
                     VGWGSKQTQFHGSEGRPTAFPVQLPENALPWDDRRPHITWRGDGQYFAVSVVCRQTEA
                     RKIRVWNREFALQSTSESVPGLGPALAWKPSGSLIASTQDKPNQQDVVFFEKNGLLHG
                     HFTLPFLKDEVKVNDLLWNADSSVLAIWLEDLPKEDSSTLKSYVQLWTVGNYHWYLKQ
                     SLPFSTTGKNQIVSLLWDPVTPCRLHVLCTGWRYLCCDWHWTTDRSSGNSANDLANVA
                     VIDGNRVLVTVFRQTVVPPPMCTYRLLIPHPVNQVIFSAHLGNDLAVLDASNQISVYK
                     CGDKPNMDSTVKLGAVGGNGFKVPLTTPHLEKRYSIQFGNNEEEEEEEVNALQLSFLT
                     WVEDDTFLAISYSHSSSQSIIHHLTVTHSEVDEEQGQLDVSSSVTVDGVVIGLCCCSK
                     TKSLAVQLADGQVLKYLWESPSLAVEPWKNSEGIPVRFVHPCTQMEVATIGGEECVLG
                     LTDRCRFFINDTEVASNITSFAVCDDFLLVTTHSHTCQVFSLSGASLKMLQAALSGSH
                     EASGEILRKVERGSRIVTVVPQDTKLILQMPRGNLEVVHHRALVLAQIRKWLDKLMFK
                     EAFECMRKLRINLNLIHDHNPKVFLENVETFVKQIDSVNHINLFFTELREEDVTKTMY
                     PPPITKSVQVSTHPDGKKLDLICDAMRAAMEAINPRKFCLSILTSHVKKTTPELEIVL
                     QKVQELQGNLPFDPESVSVEEALKYLLLLVDVNELFNHSLGTYDFNLVLMVAEKSQKD
                     PKEYLPFLNTLKKMETNYQRFTIDKYLKRYEKALGHLSKCGPEYFTECLNLIKDKNLY
                     KEALKLYRPDSPQYQAVSMAYGEHLMQEHLYEPAGLVFARCGAQEKALEAFLACGSWQ
                     QALCVAAQLQMSKDKVAGLARTLAGKLVEQRKHSEAATVLEQYAQDYEEAVLLLLEGS
                     AWEEALRLVYKYDRVDIIETSVKPSILEAQKNYMDFLDSETATFIRHKNRLQVVRALR
                     RQAPQVHVDHEVAHGPESDLFSETSSIMSGSEMSGRYSHSNSRISARSSKNRRKAERK
                     KHSLKEGSPLEGLALLEALSEVVQSVEKLKDEVRAILKVLFLFEFEEQAKELQRAFES
                     TLQLMERAVPEIWTPAGQQSSTTPVLGPSSTANSITASYQQQKTCVPALDAGVYMPPK
                     MDPRSQWKLSLLE"
     exon            337..489
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            490..571
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            572..652
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            653..738
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            739..835
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            836..926
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1050
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1144
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1375
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1540
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1640
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1832
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..1939
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2043
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2097
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2203
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2204..2319
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2393
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2472
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2552
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2690
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..2776
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..2925
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..3049
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..3147
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3148..3349
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..3411
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3412..3474
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3535
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3536..3649
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3650..3761
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3762..3889
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            3890..4044
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            4045..4120
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     exon            4121..6160
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4806..4811
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="hexamer: AATAAA"
     polyA_site      4830
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="major polyA site"
     regulatory      5048..5053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="hexamer: ATTAAA"
     polyA_site      5065
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
     regulatory      5422..5427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="hexamer: ATTATA"
     polyA_site      5443
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
     regulatory      5621..5626
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="hexamer: AATATA"
     polyA_site      5642
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
     regulatory      6092..6097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
                     /note="hexamer: AATAAG"
     polyA_site      6113
                     /gene="Elp1"
                     /gene_synonym="3110040G09Rik; 6030413P05; IKAP; Ikbkap"
ORIGIN      
        1 gacgtcactc agcgcgcctc gcgggtgacg ccacgtccgc gccgcctttc tggtgctacc
       61 tggcgtgttt cgcccttcag tggatcgtgt ttctcgtctc ccgtgtgcat cggcggttcc
      121 tttccaaacc cagtgcgccg tgagggctcg gagccgcctc gagggcgtcg tagaaattgc
      181 ttcatcatga gaaatctgaa gttgcatcgg accctggagt ttcgggacat tcaggcacca
      241 gggaaacctc agtgtttctg tctgcgagct gagcagggca cggtgctgat tggctcagaa
      301 cgtggactga cggaagtaga tcctgtgaga agagaagtga agactgaaat ttctttggtg
      361 gcagaaggtt ttcttccaga ggatggaagt ggctgcattg tgggtattca ggacttgctg
      421 gatcaggaat ctgtgtgtgt ggccaccgcc tctggggacg tcatagtctg caatctcagc
      481 acacagcagc tggaatgtgt tgggagtgtg gccagtggta tctctgtcat gagctggagt
      541 cctgatcaag aactgctgct tcttgccaca gctcaacaga ccctgattat gatgacaaaa
      601 gactttgaag tgatcgcaga ggagcagatc caccaggatg attttggaga aggcaagttt
      661 gtcactgttg gatggggcag taagcagacc cagttccacg gctcagaagg caggccaaca
      721 gcctttccgg tgcagttgcc tgagaatgcc ttgccctggg acgaccgcag accacacatt
      781 acctggcgtg gggatgggca gtattttgct gtgagtgttg tttgtcgaca aacagaggct
      841 cggaagatcc gcgtatggaa ccgagagttt gccttgcagt caaccagtga gtctgtgcca
      901 ggactggggc ccgccctggc ttggaaaccc tcaggcagtt tgattgcatc tactcaagat
      961 aaacccaacc agcaagatgt tgtgtttttt gagaaaaatg gacttcttca tggacatttt
     1021 acacttcctt tcttaaaaga tgaagttaag gtaaatgacc tgctgtggaa tgcagattcc
     1081 tcggtgcttg ctatatggct agaagacctc ccaaaggaag acagttccac tctgaaaagc
     1141 tatgtccagc tctggaccgt ggggaactat cactggtatc tgaagcaaag cctgcccttc
     1201 agcaccactg ggaagaacca gatcgtgtct ctgctgtggg acccggtgac gccgtgtcga
     1261 ctccatgttc tctgtacggg ctggcggtat ctatgctgtg actggcactg gaccactgat
     1321 cggagctctg ggaatagtgc caatgacttg gcaaatgtgg ctgttattga tggaaacagg
     1381 gtgttggtga cagtctttcg gcagacggtc gtcccacctc ccatgtgtac ctaccggctg
     1441 ctgatcccac atccagtgaa tcaagtcata ttctctgctc accttgggaa tgaccttgct
     1501 gtcctcgacg ccagtaacca gatttctgtc tacaagtgtg gtgataagcc aaacatggat
     1561 tccacagtga aactaggagc cgtgggtgga aatggattta aagttcctct tacaacacct
     1621 catttggaaa agagatactc gattcagttc gggaacaatg aagaagaaga agaagaagaa
     1681 gtaaacgcac tgcagttgag ctttctcacc tgggttgaag acgacacttt cctggctata
     1741 agctacagtc actccagctc acagtccatc attcaccatt tgactgtgac ccattctgag
     1801 gtggatgagg agcaaggcca gctggatgtc agttcgtctg tgacagtgga tggagtcgtc
     1861 attggtttat gctgctgttc taagaccaag tcattagcag tacagctggc tgatggccag
     1921 gtgctcaagt acctttggga gtcaccttct ctggctgtgg aaccctggaa gaattctgaa
     1981 ggaattcctg ttcgatttgt tcatccatgc acacagatgg aagtagccac gattggagga
     2041 gaggaatgtg tccttggtct gactgacagg tgtcgctttt ttatcaatga cactgaggtc
     2101 gcatcaaata tcacgtcatt tgctgtgtgt gatgactttc tactggtgac aacccattcc
     2161 cacacctgcc aggttttctc cctaagtggt gcctcactta aaatgctaca ggcagctctg
     2221 agtggcagtc atgaggccag tggagaaatt ctgcggaaag tggaaagggg ttcacggatc
     2281 gtgacagttg ttccccagga cacaaagctt atactacaga tgccaagggg aaacctggag
     2341 gttgttcatc atcgggcctt ggtcttggca cagattcgga agtggctgga caaacttatg
     2401 tttaaagaag catttgaatg catgaggaaa ctgagaatta acctcaatct gattcatgac
     2461 cataacccca aggtctttct cgaaaatgtg gaaaccttcg taaaacagat tgattctgta
     2521 aatcatataa atttgttttt cacagaactc agggaagaag atgtcacaaa gaccatgtac
     2581 cctcccccga ttaccaagag tgtccaggtg tccacacatc ctgatgggaa gaaacttgac
     2641 ctcatctgtg acgccatgag agcagccatg gaggccatca atcctcgaaa gttctgcctg
     2701 tcgatactca catctcatgt gaagaaaacc accccggagc tggaaattgt gctgcagaag
     2761 gtacaggaac ttcaaggaaa tcttcccttt gatcctgaga gtgtgagtgt agaggaggct
     2821 ttgaaatacc tgctgctcct ggtggatgtt aatgagttat ttaatcactc ccttgggacc
     2881 tatgacttta atttagtcct catggtagct gagaagtcac aaaaggatcc taaagaatat
     2941 cttccatttc tgaatacact taagaaaatg gaaactaatt accagaggtt caccatagac
     3001 aagtacttga aacgatatga aaaggccctt ggacacctca gcaagtgtgg acctgagtat
     3061 ttcacagaat gcttaaattt gataaaagat aaaaacttgt ataaagaagc tctcaagtta
     3121 tatcgtccag actcaccaca gtaccaggcg gtcagcatgg cttatggcga gcacctgatg
     3181 caggagcacc tgtacgagcc agcagggctg gtgtttgccc gctgtggggc ccaggagaag
     3241 gcacttgaag catttttggc ttgtggcagc tggcagcaag ccctctgtgt ggctgcccag
     3301 cttcagatgt ccaaagacaa ggtggctggc cttgctagaa ctctggcagg aaagctggtg
     3361 gagcagagga agcacagtga ggcagccaca gtcctggaac agtacgccca ggattatgaa
     3421 gaggctgtgc tcctgctgct agaaggatct gcctgggaag aagctctgag gctggtctac
     3481 aagtatgaca gagtggatat tatagaaacc agtgtaaagc cttccatttt agaagcccaa
     3541 aaaaattaca tggactttct ggactctgag acagccacat tcattcgcca taaaaaccgc
     3601 ttgcaggtgg ttcgagcgct caggaggcaa gcaccacagg tccatgtgga tcacgaggtg
     3661 gcccatggcc cagagtcaga cctcttctcg gaaaccagca gtattatgag tggcagtgag
     3721 atgagtggca gatactccca cagtaactcc aggatatctg cgaggtcatc taaaaaccgt
     3781 cggaaggcag agcgcaagaa gcatagcctc aaagaaggga gtcctctgga gggcctggct
     3841 cttctggagg cgctgagtga agtggtacaa agtgttgaga agctaaaaga tgaggtgcgt
     3901 gctattttga aggtgctctt tctctttgag tttgaagagc aagcaaagga gttacagaga
     3961 gcctttgaga gtactctgca gctgatggag agggcagttc cagagatctg gactcctgcc
     4021 ggccaacaga gttcaaccac accggtccta ggtcccagct ccactgcgaa tagcatcaca
     4081 gcctcttacc agcagcagaa gacttgtgtc cctgctctcg atgctggggt ttatatgcca
     4141 ccgaagatgg acccgagaag ccagtggaag ctgagcctgc tggaatgact gccctgttag
     4201 gaaggccttg ccgggaagac cgtttgtcca ctcactcttc gtgcttctag ctgtggagaa
     4261 ctgggatgtg gaagagtctg cctttgaaca gcctcagcta gcactaggca gtccccactt
     4321 tcttttgggt atacatctta ggtactatgt cagaacttcg ttttagccag cttattgcag
     4381 tctttttaaa aattgctgtt tagctggcta tagtaggaca tgcctataat cccagcagcc
     4441 caggaagctg gggcagaaag actgatgcaa gttcaagacc agcatggcct tatctcaaaa
     4501 caacccatcc tgctgttctt gtactcagta ctgtttgaaa gcatggttag gcagatcatg
     4561 aactggagtc tcgtttctgt tacaaccttg gacagctcat cattgatttg atcctcagtt
     4621 cctgttgttc atagggaatg atgtttcatg gtttctacct caaaaagtga agacaagtgg
     4681 aaccacgttt ctaagttgct ctgtgttgtt aacatagtgc atggtattca tgtaggcagt
     4741 tagtgctgcc agcttttagt aagatgtgat tgcatagctg gcatctgtgc attaactcaa
     4801 agcagaataa aaagctgttt ggggactgga gaattgactc agtggtaaag agcggttgtt
     4861 gcttttgcag tggacctggg tttcccagca cccacatagc agctcacaac ggtctgtttt
     4921 ctccagttct agacgatcta acgtcctctt cggatttcac tgagcaccag gcaagcatgt
     4981 ggtacacaaa catatgtgca ggcaaaatac tcatacacac aaaataatct ttaaaaaatt
     5041 ttttttaatt aaatatgtta tcctaccttg ttatgtgcta ctagctttaa ttgggctaga
     5101 cttcccctag ctggcactgt tccagggatg ttctattgcc tcagtatgtt ctgctagaga
     5161 gttaccaaga gtagggtgga cttcagtgat gggtagacag caggcatatg tgggagcagg
     5221 gttggttgtt actctgtccc ccaaacacta cctcctttga ctaacgcatt ctgtctgtgt
     5281 ccaggcagac tcacttggct ttgtgtatgg ctttttgtat gacatcagat gcggagatgc
     5341 agagtctaga gtttgcctag ctggttcagt ctttcagtaa ctctgcttcc tcctcaattt
     5401 tggaactgta taccctatgc tattatatgt tggaagtatg tgatttgctt tttggttttg
     5461 attttacaga agattacaaa taagagattg cataaatctt agaagaaact ttggactttt
     5521 aaacactgtt gagagagtgt tagtctatgg gaacttttga agtaggacta aatgcatttt
     5581 acattatgta tggctgcaag cctgtggggg ccaggagtgg aatatagtgt tttgaatagg
     5641 aatggcctcc atagactcat gtgtttgaac ccttggcccg tgggcagtgg cactattagg
     5701 cgtggccttg ttggtgtaga tgtccttgtt ggaggaagtc tgtcactgtg gaggtgtgct
     5761 ttgagatctt ccatgcttag gctacaccca gtgtagcaga gtctctgctg ctaacttctg
     5821 atcaagatag aactcttgcc ttgtacagca ctgtttctgc ctgcaggctg ccatgcttcc
     5881 tgccatgaca ataatggact aaacctctga gactgtaagc cagccccaaa gaaatgtttc
     5941 tccatgatag ttggtatggt cttggtgtct cttcatagca atgaaaccct aagacattga
     6001 ctgacccatg tttcattgtt tccaagagat ctttggaaga tgagattact ttctctctct
     6061 tgtcaagtct agaaattggt cagtttattt taataagaga ttttgaaaaa ttactgtttg
     6121 acaattttat atttttacat aatgaatttg atttgttctc
//
