LOCUS       NM_015779                920 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus elastase, neutrophil expressed (Elane), mRNA.
ACCESSION   NM_015779
VERSION     NM_015779.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Lulla,A.R., Akli,S., Karakas,C., Caruso,J.A., Warma,L.D.,
            Fowlkes,N.W., Rao,X., Wang,J., Hunt,K.K., Watowich,S.S. and
            Keyomarsi,K.
  TITLE     Neutrophil Elastase Remodels Mammary Tumors to Facilitate Lung
            Metastasis
  JOURNAL   Mol Cancer Ther 23 (4), 492-506 (2024)
   PUBMED   37796181
  REMARK    GeneRIF: Neutrophil Elastase Remodels Mammary Tumors to Facilitate
            Lung Metastasis.
REFERENCE   2  (bases 1 to 920)
  AUTHORS   Zhang,H., Wu,D., Wang,Y., Shi,Y., Shao,Y., Zeng,F., Spencer,C.B.,
            Ortoga,L., Wu,D. and Miao,C.
  TITLE     Ferritin-mediated neutrophil extracellular traps formation and
            cytokine storm via macrophage scavenger receptor in
            sepsis-associated lung injury
  JOURNAL   Cell Commun Signal 22 (1), 97 (2024)
   PUBMED   38308264
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 920)
  AUTHORS   Liu,C., Yalavarthi,S., Tambralli,A., Zeng,L., Rysenga,C.E.,
            Alizadeh,N., Hudgins,L., Liang,W., NaveenKumar,S.K., Shi,H.,
            Shelef,M.A., Atkins,K.B., Pennathur,S. and Knight,J.S.
  TITLE     Inhibition of neutrophil extracellular trap formation alleviates
            vascular dysfunction in type 1 diabetic mice
  JOURNAL   Sci Adv 9 (43), eadj1019 (2023)
   PUBMED   37878711
REFERENCE   4  (bases 1 to 920)
  AUTHORS   Yang,M., Zhou,X., Pearce,S.W.A., Yang,Z., Chen,Q., Niu,K., Liu,C.,
            Luo,J., Li,D., Shao,Y., Zhang,C., Chen,D., Wu,Q., Cutillas,P.R.,
            Zhao,L., Xiao,Q. and Zhang,L.
  TITLE     Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection
            in Mice
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (10), 1900-1920 (2023)
   PUBMED   37589142
  REMARK    GeneRIF: Causal Role for Neutrophil Elastase in Thoracic Aortic
            Dissection in Mice.
REFERENCE   5  (bases 1 to 920)
  AUTHORS   Tsai,C.H., Lai,A.C., Lin,Y.C., Chi,P.Y., Chen,Y.C., Yang,Y.H.,
            Chen,C.H., Shen,S.Y., Hwang,T.L., Su,M.W., Hsu,I.L., Huang,Y.C.,
            Maitland-van der Zee,A.H., McGeachie,M.J., Tantisira,K.G.,
            Chang,Y.J. and Lee,Y.L.
  TITLE     Neutrophil extracellular trap production and CCL4L2 expression
            influence corticosteroid response in asthma
  JOURNAL   Sci Transl Med 15 (699), eadf3843 (2023)
   PUBMED   37285400
REFERENCE   6  (bases 1 to 920)
  AUTHORS   Iwama,A., Zhang,P., Darlington,G.J., McKercher,S.R., Maki,R. and
            Tenen,D.G.
  TITLE     Use of RDA analysis of knockout mice to identify myeloid genes
            regulated in vivo by PU.1 and C/EBPalpha
  JOURNAL   Nucleic Acids Res 26 (12), 3034-3043 (1998)
   PUBMED   9611252
REFERENCE   7  (bases 1 to 920)
  AUTHORS   Belaaouaj,A., McCarthy,R., Baumann,M., Gao,Z., Ley,T.J.,
            Abraham,S.N. and Shapiro,S.D.
  TITLE     Mice lacking neutrophil elastase reveal impaired host defense
            against gram negative bacterial sepsis
  JOURNAL   Nat Med 4 (5), 615-618 (1998)
   PUBMED   9585238
REFERENCE   8  (bases 1 to 920)
  AUTHORS   Sturrock,A., Franklin,K.F., Wu,S. and Hoidal,J.R.
  TITLE     Characterization and localization of the genes for mouse
            proteinase-3 (Prtn3) and neutrophil elastase (Ela2)
  JOURNAL   Cytogenet Cell Genet 83 (1-2), 104-108 (1998)
   PUBMED   9925946
REFERENCE   9  (bases 1 to 920)
  AUTHORS   Belaaouaj,A., Walsh,B.C., Jenkins,N.A., Copeland,N.G. and
            Shapiro,S.D.
  TITLE     Characterization of the mouse neutrophil elastase gene and
            localization to chromosome 10
  JOURNAL   Mamm Genome 8 (1), 5-8 (1997)
   PUBMED   9021140
REFERENCE   10 (bases 1 to 920)
  AUTHORS   Nuchprayoon,I., Meyers,S., Scott,L.M., Suzow,J., Hiebert,S. and
            Friedman,A.D.
  TITLE     PEBP2/CBF, the murine homolog of the human myeloid AML1 and PEBP2
            beta/CBF beta proto-oncoproteins, regulates the murine
            myeloperoxidase and neutrophil elastase genes in immature myeloid
            cells
  JOURNAL   Mol Cell Biol 14 (8), 5558-5568 (1994)
   PUBMED   8035830
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK143710.1 and DY245521.1.
            
            On Sep 6, 2006 this sequence version replaced NM_015779.1.
            
            Summary: This gene encodes a member of the chymotrypsin-like family
            of serine protease enzymes that hydrolyzes a broad range of protein
            substrates including elastin. This gene is expressed by neutrophils
            where the encoded enzyme is stored in azurophil granules. Upon
            neutrophil activation, the active enzyme is released into the
            extracellular mileu. Mice lacking the encoded protein exhibit
            increased susceptibility to sepsis and death following
            intraperitoneal infection with Gram negative bacteria. This gene is
            located adjacent to a related proteinase gene on chromosome 10.
            [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK143710.1, BC145800.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-394               AK143710.1         1-394
            395-472             DY245521.1         1-78                c
            473-920             AK143710.1         473-920
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..920
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /note="elastase, neutrophil expressed"
                     /db_xref="GeneID:50701"
                     /db_xref="MGI:MGI:2679229"
     exon            1..87
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..821
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /EC_number="3.4.21.37"
                     /note="leukocyte elastase; elastase 2, neutrophil"
                     /codon_start=1
                     /product="neutrophil elastase precursor"
                     /protein_id="NP_056594.2"
                     /db_xref="CCDS:CCDS23994.1"
                     /db_xref="GeneID:50701"
                     /db_xref="MGI:MGI:2679229"
                     /translation="MALGRLSSRTLAAMLLALFLGGPALASEIVGGRPARPHAWPFMA
                     SLQRRGGHFCGATLIARNFVMSAAHCVNGLNFRSVQVVLGAHDLRRQERTRQTFSVQR
                     IFENGFDPSQLLNDIVIIQLNGSATINANVQVAQLPAQGQGVGDRTPCLAMGWGRLGT
                     NRPSPSVLQELNVTVVTNMCRRRVNVCTLVPRRQAGICFGDSGGPLVCNNLVQGIDSF
                     IRGGCGSGLYPDAFAPVAEFADWINSIIRSHNDHLLTHPKDREGRTN"
     exon            88..244
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /inference="alignment:Splign:2.1.0"
     exon            245..386
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /inference="alignment:Splign:2.1.0"
     exon            387..620
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /inference="alignment:Splign:2.1.0"
     exon            621..920
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      898..903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /note="putative"
     polyA_site      920
                     /gene="Elane"
                     /gene_synonym="Ela2; F430011M15Rik; NE"
                     /note="putative"
ORIGIN      
        1 gagccgcgag aacacagccc accatggccc ttggcagact atccagccgg actctggctg
       61 ccatgctact ggcattgttc ctgggtggcc cagcactggc ctcagagatt gttggtggcc
      121 ggccggcccg gccccatgct tggcccttca tggcatccct gcagaggcgt ggaggtcatt
      181 tctgtggtgc caccctcatt gccaggaact tcgtcatgtc agcagcccac tgtgtgaacg
      241 gcctaaattt ccggtcagtg caggtagtgc tgggagccca tgacctgcgg cgacaggagc
      301 gcactcgaca gaccttctct gtgcagcgga tcttcgagaa tggctttgac ccatcacaac
      361 tgctgaacga cattgtgatt atccagctca atggctccgc taccattaac gccaacgtgc
      421 aggtggccca gctgcctgcc cagggccagg gcgtgggtga cagaactcca tgtctggcca
      481 tgggctgggg caggttgggc acaaacagac catcacccag tgtgctacaa gagctcaatg
      541 tgacagtggt gactaacatg tgccgccgtc gtgtgaacgt atgcactctg gtgccacgtc
      601 ggcaggcagg catctgcttc ggggactctg gcggaccctt ggtctgtaac aaccttgtcc
      661 aaggcattga ctccttcatc cgaggaggct gtggatctgg attgtaccca gatgccttcg
      721 cccctgtggc tgagtttgca gattggatca attccattat ccgaagccat aatgaccacc
      781 ttcttaccca tcccaaagac cgagagggca ggaccaacta ggacctgtct gcactcccac
      841 cccgctcccc ttgctccctt gctcccccct ctaccagaga cctctccatt ttactacaat
      901 aaaagcagcc tttgttttgt
//
