LOCUS       NM_198303               7797 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus eukaryotic translation initiation factor 5B (Eif5b),
            mRNA.
ACCESSION   NM_198303
VERSION     NM_198303.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Distler,U., Schmeisser,M.J., Pelosi,A., Reim,D., Kuharev,J.,
            Weiczner,R., Baumgart,J., Boeckers,T.M., Nitsch,R., Vogt,J. and
            Tenzer,S.
  TITLE     In-depth protein profiling of the postsynaptic density from mouse
            hippocampus using data-independent acquisition proteomics
  JOURNAL   Proteomics 14 (21-22), 2607-2613 (2014)
   PUBMED   25211037
REFERENCE   3  (bases 1 to 7797)
  AUTHORS   Lee,S., Truesdell,S.S., Bukhari,S.I., Lee,J.H., LeTonqueze,O. and
            Vasudevan,S.
  TITLE     Upregulation of eIF5B controls cell-cycle arrest and specific
            developmental stages
  JOURNAL   Proc Natl Acad Sci U S A 111 (41), E4315-E4322 (2014)
   PUBMED   25261552
  REMARK    GeneRIF: eIF5B overexpression promotes maturation of G0-like
            immature oocytes and causes cell death, an alternative to G0, in
            serum-starved THP1 cells.
REFERENCE   4  (bases 1 to 7797)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   5  (bases 1 to 7797)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 7797)
  AUTHORS   Bohnsack,M.T., Regener,K., Schwappach,B., Saffrich,R.,
            Paraskeva,E., Hartmann,E. and Gorlich,D.
  TITLE     Exp5 exports eEF1A via tRNA from nuclei and synergizes with other
            transport pathways to confine translation to the cytoplasm
  JOURNAL   EMBO J 21 (22), 6205-6215 (2002)
   PUBMED   12426392
  REMARK    GeneRIF: eIF5B, the homologue of bacterial IF2, is a GTPase
            implicated in joining the ribosomal subunits.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK145732.1, BC103806.1, CN724918.1, CN670500.1 and AK173016.1.
            
            On Dec 29, 2005 this sequence version replaced NM_198303.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164131,
                              SAMN01164132 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-526               AK145732.1         2-527
            527-1664            BC103806.1         408-1545
            1665-2068           CN724918.1         185-588
            2069-2483           CN670500.1         102-516
            2484-3661           BC103806.1         3237-4414
            3662-7797           AK173016.1         735-4870
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 16.4 cM"
     gene            1..7797
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="eukaryotic translation initiation factor 5B"
                     /db_xref="GeneID:226982"
                     /db_xref="MGI:MGI:2441772"
     exon            1..315
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..3931
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /EC_number="3.6.5.3"
                     /note="eIF-5B; translation initiation factor IF-2"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 5B"
                     /protein_id="NP_938045.2"
                     /db_xref="CCDS:CCDS35544.1"
                     /db_xref="GeneID:226982"
                     /db_xref="MGI:MGI:2441772"
                     /translation="MGKKQKNKSEDSTKDDTDLGALAAEIEGAGAAKEQEPQKSKGKK
                     KKEKKKQDFDENDILRELEELSLEAQGIRADRDAAAVKPTENNEEESASKQDKKKKGQ
                     KGKKTSFDENDSEELEDKDSKSKKTARPNSEAPLSGSEDADDSNKLSKKGKKAQKSTK
                     KRDGSEEDEDNSKRSKERSRVNSSGESGGESDEFLQSRKGQKKNQKNKSVPTVDSGNE
                     DDDSSFKIKTVAQKKAEKKEREKKKRDEEKAKLRKMKEKEELEKGKKEQSKQREPQKR
                     PEEEVLTLRGTPDTGAASEEKGDTAAALEDDNEGDKKKKDKKKKKTEKDEKEKEKKKG
                     PSKSTVKAIQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQK
                     EKERKERLKKEGKLLTKSQREARARAEVTLRHLQAQGVEVPSKDSLPKKRPVYEDKKK
                     KKTPQQLESKEVSETLEISAPVEAVDQGGPEKEETPPSVEPEEEEDTEDAGLDDWEAM
                     ASDEEREKEGNMIHIEVEENPEEEEEEEEEEEEEESEDEEEEGDSEGSDGDEEDCKLS
                     DEKDSGKAGDTKPSKDASSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHGKNVNTEK
                     LRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIK
                     NFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINIL
                     KSKKCPFIVALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKDEFEERAKAIIVEFAQQG
                     LNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQV
                     MEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQ
                     YEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKDDEIPVLKDELIHELKQTLNAIKL
                     EEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVI
                     LAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPC
                     KMKILPQYIFNSRDPIVIGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDV
                     AKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQ
                     LIVELKKVFEII"
     exon            316..441
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            442..526
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            527..1196
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1414
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1565
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1664
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1754
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1829
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..2110
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2218
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2329
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2397
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2539
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2671
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2854
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..3016
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3148
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3149..3280
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3281..3357
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3358..3522
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3523..3661
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3662..3823
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..7797
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4211..4216
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="hexamer: ATTAAA"
     polyA_site      4239
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="major polyA site"
     regulatory      4463..4468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="hexamer: AATAAA"
     polyA_site      4487
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
     regulatory      5365..5370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="hexamer: AATAAA"
     polyA_site      5388
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
     regulatory      5824..5829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="hexamer: AATAAA"
     polyA_site      5849
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
     regulatory      6700..6705
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="hexamer: AATAAA"
     polyA_site      6720
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
     regulatory      7585..7590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
                     /note="hexamer: AATAAA"
     polyA_site      7628
                     /gene="Eif5b"
                     /gene_synonym="A030003E17Rik; IF2"
ORIGIN      
        1 acctggccgg cggctctact ggagacgctc tagccgaagc gccgctctcg atgagccgcg
       61 ccctgcgtcc ccacgccgag gcggcggggc gatgcgcttg cgtactgagg gctcacacca
      121 tatgtgccct gtcccagtgc gcgggtctgt ggagagccgg gtgcgagagg cggcggcgcg
      181 agggggaccg agccgagcgg gcattggagt cagccgggag acagtgggtc tgtgagacac
      241 cgaaccgagg ggccggggcc gcgagcgccg ttgacaagcg atggggaaga aacagaaaaa
      301 caagagcgaa gacagtacca aggatgatac tgatttgggc gccttggctg cagaaataga
      361 aggagctggt gctgccaagg agcaggagcc tcagaagtca aagggaaaga agaaaaagga
      421 gaaaaagaaa caagactttg atgagaacga cattctgaga gagctggagg agttgtctct
      481 ggaagcccaa ggcatcagag ctgacagaga cgccgctgca gtcaagccta cagaaaacaa
      541 tgaagaagaa tctgcctcaa aacaagataa aaagaagaag ggacaaaaag gcaagaaaac
      601 aagttttgat gagaatgata gtgaagaatt ggaagataaa gattcaaagt caaaaaagac
      661 tgccagacct aactcagagg cacccctttc tgggagtgag gatgctgatg attctaataa
      721 actttctaaa aaaggtaaaa aagcccagaa atccacgaaa aaacgggacg ggtcagaaga
      781 ggatgaagac aatagcaaaa gaagtaaaga acgttcgaga gtaaactcct ctggtgaaag
      841 tggtggtgag tccgatgaat ttttgcagtc cagaaaagga cagaaaaaaa atcagaagaa
      901 caagtccgtt cctaccgtag acagtgggaa tgaggacgat gactcttcct tcaaaattaa
      961 gacggtggcc cagaagaagg cagaaaagaa agagcgcgag aagaaaaaga gagatgaaga
     1021 gaaagcgaag ttgcggaaga tgaaggagaa agaagagcta gagaagggca agaaggagca
     1081 gagtaaacag agggagcctc agaagagacc tgaggaggag gtgttgacac tcagagggac
     1141 tcctgacaca ggagctgcct cggaagagaa aggagacact gctgcagctc tggaagacga
     1201 caatgaagga gacaaaaaga aaaaagataa gaagaaaaag aaaacagaga aagatgagaa
     1261 agaaaaagag aagaagaaag gccctagtaa atctactgtt aaagctatcc aggaagcttt
     1321 ggctaagctc aaagaggagg aggagagaca gaagagagag gaggaagaaa ggattaagcg
     1381 gcttgaggag cttgaagcca aacgaaaaga agaggaacga ttagaacaag aaaaaagaga
     1441 aaggaaaaag caaaaagaaa aagaaagaaa agaacggtta aaaaaagaag gaaagctgtt
     1501 aactaagtcc cagagagaag ccagagccag agccgaagtg acccttagac acctccaagc
     1561 tcagggtgtt gaagtgccat caaaagactc gttgccaaag aaaaggccag tttatgaaga
     1621 taagaagaag aagaaaacac cacagcagct agagagtaaa gaagtatctg aaactttgga
     1681 aataagtgct cctgtggaag ctgtggacca aggaggacca gaaaaggaag agacgccacc
     1741 ttctgttgag ccagaggaag aggaagacac cgaagatgct ggcttagatg actgggaggc
     1801 tatggccagt gatgaggaga gagaaaaaga aggaaatatg attcacatag aagtagaaga
     1861 aaaccctgag gaagaagaag aggaagaaga agaggaggag gaagaagaga gtgaagatga
     1921 agaagaggaa ggggacagtg aaggcagcga tggggacgag gaggactgca agctgtcgga
     1981 tgagaaggac tcagggaagg ctggagacac gaagcccagc aaagacgcta gctcagactc
     2041 cgagtacgac tccgacgacg atcggactaa agaagagcgg gcgtacgaca aagcgaaacg
     2101 gaggattgag aaacgacgac ttgaacatgg taaaaatgta aatacagaaa agctgagagc
     2161 ccctatcatc tgtgtgcttg gacatgtaga cacaggaaag acaaaaattc tagataagct
     2221 ccgtcacaca catgtgcaag atggcgaagc aggtggtatt acacagcaga ttggtgccac
     2281 aaatgttcct cttgaagcta ttaatgaaca aactaagatg attaaaaatt ttgacagaga
     2341 gaatgtccga atcccaggga tgctgattat tgatactcct ggacacgagt ctttcagtaa
     2401 cctgagaaac agaggaagct ctctttgtga cattgccatt ttagttgttg acattatgca
     2461 tggtttagag ccccagacaa ttgaatcaat caacattctc aaatctaaaa agtgcccctt
     2521 cattgttgca cttaataaga ttgacaggtt atatgattgg aagaagagtc ctgattctga
     2581 tgtggctgtt actttaaaaa agcagaagaa gaatacaaag gatgaatttg aggagcgtgc
     2641 aaaggccatt attgtagaat ttgcacagca gggtttgaat gctgctttgt tttatgagaa
     2701 taaagatccc cgcactttcg tgtccttggt acctacctct gcacatactg gtgatggcat
     2761 gggaagtctc atctaccttc ttgtggagtt aactcaaacc atgttgagca agaggcttgc
     2821 acactgtgaa gagctgagag cacaagtcat ggaggttaaa gctctcccag gaatgggcac
     2881 cacaatagat gtcatattga tcaatggacg tttgaaggaa ggggatacaa tcattgtccc
     2941 tggagtagaa gggcccattg tcactcagat tcgaggcctc cttttacctc ctcctatgaa
     3001 ggaattacgt gtgaagaacc aatatgaaaa gcataaagaa gtggaagcgg cccagggagt
     3061 aaagattctt gggaaagacc ttgaaaaaac cctggctggc ttacctctcc tagtggccta
     3121 caaggatgat gaaatcccag ttctcaaaga tgaattgatc catgagttaa agcagacact
     3181 aaatgctatc aaactagaag aaaaaggagt ttatgtccag gcatctacac tgggctcttt
     3241 ggaagctctt cttgaatttc tgaaaacatc agaggtgcca tatgcaggaa ttaacattgg
     3301 cccagtccat aaaaaggacg ttatgaaggc ttcagtgatg ttggaacatg atccccagta
     3361 tgcagtaatt ttggcctttg atgtaagaat tgaacgggac gcacaagaga tggctgatag
     3421 tttgggagtt agaatcttta gtgcagaaat tatttatcac ttatttgatg cctttacaaa
     3481 atacagacag gactacaaaa aacaaaaaca agaagaattt aagcacatag cagtatttcc
     3541 ctgcaagatg aaaatcctcc ctcaatacat tttcaattct cgagatccaa tagtaatagg
     3601 ggtcactgtg gaggctggtc aagtgaaaca aggaacacct atgtgcgttc caagcaaaaa
     3661 ttttgttgac attgggatag taacaagcat tgaaataaac cacaagcagg tggatgttgc
     3721 aaaaaaagga caagaggtct gtgtcaaaat agaacctatc cctggggaat ctcccaaaat
     3781 gtttgggaga cattttgaag ctacagatat acttgtcagc aagatcagcc ggcagtccat
     3841 cgatgcactc aaagactggt tcagagacga aatgcagaag agtgactggc agcttattgt
     3901 ggagctgaag aaagtatttg aaatcatcta attttcccac ctgggacaga atttggagtg
     3961 atgtaatatt atgttgtgat attaccaacg gaacagacgt ttggacgctg atggacttag
     4021 gtgtataaaa tgttttccat gagaaaccaa gcaacttaca ctggtttgac agtggtcagt
     4081 tccccgcccc acagtccagc gtgcctgcac tcacccaccc ttcctttaag acttggctgc
     4141 tgttttaaag tttgcccttc cttcaaattt ggatttttat tacaaatcta aatctttcaa
     4201 ttttatactg attaaatcag tcctgcagta tttgattaac caagcttcta cagattttgt
     4261 gattcttggg actttttcga tgtaagaaat aagtttatgg gtattctttc cacagtgact
     4321 tacccttctc ccacccttag cgacttttct aactagaaaa tgtaggtagg gacttcgctt
     4381 tttccttctg ctctgtgtgc agccatggct ggctacaatt actgcttgta ctagtcagga
     4441 gagctcattg gaacaaactg ccaataaaga ggagacactg cagtcgagta ggttctgcta
     4501 ggcctccatt ctagctcatc agcacctgct aacgcttgcc ctcagagggg cgctctgcat
     4561 acattccatt gtttgcttct gcatgattag acctcagtga ttcatccaca cacctcatga
     4621 ctagactgca tagttactcc tgaggaccca atagcatcct ttgaagaaac ctgttgtagg
     4681 agaacttgaa gttcagagtg ttcattcgtt caaagtatcc actacaaatg aatgtattat
     4741 aagaaatgtg ttatgacaca gcacacacta gagaagagat attattttct caacatcatc
     4801 tcgaacttga gttaggtaat aaatcccagg ctaggatttt attggttttc cttgttcaca
     4861 aaactagtaa aatgctggca ttggactgtt gagcaatttc acagcttttg gacatctgta
     4921 gcaggctggc ttggaggtcc agcctttaag tagaataaac tgttgctagt tcctttaggg
     4981 attcacaaga aaaaaaaaac ctgtacaaaa tatacataca gttctttatt aaacaactgt
     5041 aaacacttca ctgtaaaaat ccataaaact ttataaacaa acgttttgta aatagaatct
     5101 atgctacaag taaaagaatt aacacaatta tttacatgca atactgacaa atttggcact
     5161 tttttgaaaa gaaatgtaca aaacacttgc ttaaaaagaa atgtagaatt atgaaaactc
     5221 agagcatcac tatcatgcac tttgcaaata cctcacaagc actgctggca cagcgcagag
     5281 agccccaggc cctctgcaca gcagtcaggt cactttcagt gtgcttccat aagtctgctg
     5341 taaaaccacc tgaacattgt caagaataaa gtcaaacgcc atgttccata cagactccac
     5401 cgactgctgc atcaacctga aggtgaggaa gaggcaccac tgaagagaaa gctccagcac
     5461 gcatggttgt ccttcagact tagcttcatt cttggttttt tttttctttt tttttttgaa
     5521 ctggatacag tggctcacgc ctttaatctc agccactgag tttaaaggcc agcccagaca
     5581 gtaaaaccaa gtgttaagaa aataaacttg gtttaaaagg cctattaggg cactcagaaa
     5641 catttcatgg tcacttgtgt attggacata taaccatggg agtgattatt tttaacttag
     5701 tattcctgtc aacattatta tatggcattt aaaaccagga accttaaaaa cctgttctat
     5761 ccatatgccc tcaattgact gttgggacat gctttttttt tttcctcttt aagtaaaaaa
     5821 ttaaataaat taccttttca tgtattttat aaccagatcc aatttttcca agtctttctc
     5881 ctctatgagg tcagtgcagt atctcacaac ctgcaggatg tcttcttcca tcggatctag
     5941 ggaggaggaa aaccccaggt gaggacccag cacgccttac agcttgtaat gttagacaag
     6001 gggctcgagc cattgtgctc aggtcctgtt ctcgaatcac agagctacca cacaaacctg
     6061 agatagtagt gatccactct ttgagcaagg tcttcacatc actgaattcc acagctccag
     6121 ctaggttggg tgctgctggt ctaaaacagc tgctctggcc tggcagtaaa ctagataggt
     6181 cctgtgcacc aggggtagaa gccgagactt cttcctgttg aggaagtgaa cgagaatgct
     6241 gtattcagac agtgtcaagt acattaaaat ctagtaacag tcagtatttt gttccagtta
     6301 ccaggggtct ctctgaagcc attccttcat gttgtagaaa accatccatt aacttctggg
     6361 ggcttccata tgcccctggc agagtttttg caggactgct aagcagctta tttttcaaag
     6421 gactctttct gggagaacca atgaggttct tcctcttgtt cctcttgtct ttcattgctg
     6481 gtggctttag ctgaagtaaa ggattctttg gcactaaaag aggaggtatg agagtctcaa
     6541 aatgcaatca ggtaataata ccattgactc tttgtccttc caaccccccc ttacaaccaa
     6601 gagcaggaaa gaaggccagg cacgccgtgg cttaaggcat aaagtcctcc gagctcagct
     6661 gtacaacttc gtattctcat ttagcagtta cgtaggttta ataaaatgtg tgttcaggga
     6721 ctcatgtttt gagatttcat gttttgaacc cacattcttt caaacagcag tctcaaagga
     6781 caagtagctt gacttgtatc caaaaatact aagaccagca caagtaccaa ccagcagtga
     6841 cttacagcac tcgctactcg tctgccttga tcacttcaga cactctctag gatgaacagt
     6901 tataacacaa tgatgtcatc accctagtcc ctttttggga catgacagtt ctctcctctt
     6961 accagatgta ctggttggct gctgatgagt agtgtcctct ccctgccttt gtctttgatc
     7021 atatgctgct ttcagctcct tttgaagctc agcaggaagg gcagcaaaga catcagggtc
     7081 cacctagtac aaggaaagtc acacctaaat taattctgga ggacagctag actgttttca
     7141 aatcacttta acaggtcctt aaacagcaaa gctaggacaa aaccttccaa gacactggat
     7201 atgagcaatc caatttctgt gctaactaat cagaacaaga cactcggtta caaaatggga
     7261 agccaaaaac acatggacac ttctgggcat cttcccctat tcaggaggtc tcatgatctg
     7321 tgtgaaccag taaagtcaac aaagagtgtc ttccagaatc tttagggacc aacacctgcc
     7381 tcccacatct cttccctctg gtgctgaggt gccaaccact gtctgcagaa gtgcaagctc
     7441 ctcaggaatt agatagagga gaggacaggt gagaaaagca tgactgaagg tccctgacat
     7501 caacgtactt gtgagaatgc tggaagggca attacactaa tcttgctgtc actattacaa
     7561 ggttcttgag gctctgggat ctgtaataaa actgttccaa caggatgtgg caacacccct
     7621 gaactgcagc catttactgg ttctttcttc tttccacgtg gctctccctg ctgagcggca
     7681 caaacttgtt ctatttgttc ccggagatca agtgggagcg cttctaaaac agactggtca
     7741 atctgtaaaa taccaaatgt gaacttgatg ctcagaaata aaagtttcag gaacagt
//
