LOCUS       NM_181582               1361 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus eukaryotic translation initiation factor 5A (Eif5a),
            transcript variant 5, mRNA.
ACCESSION   NM_181582 NM_181583
VERSION     NM_181582.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Xiong,H., Ye,J., Luo,Q., Li,W., Xu,N. and Yang,H.
  TITLE     Exosomal EIF5A derived from Lewis lung carcinoma induced adipocyte
            wasting in cancer cachexia
  JOURNAL   Cell Signal 112, 110901 (2023)
   PUBMED   37743008
  REMARK    GeneRIF: Exosomal EIF5A derived from Lewis lung carcinoma induced
            adipocyte wasting in cancer cachexia.
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Zeng,J., Ye,Z., Shi,S., Liang,Y., Meng,Q., Zhang,Q. and Le,A.D.
  TITLE     Targeted inhibition of eIF5Ahpu suppresses tumor growth and
            polarization of M2-like tumor-associated macrophages in oral cancer
  JOURNAL   Cell Death Dis 14 (8), 579 (2023)
   PUBMED   37653021
  REMARK    GeneRIF: Targeted inhibition of eIF5A[hpu] suppresses tumor growth
            and polarization of M2-like tumor-associated macrophages in oral
            cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1361)
  AUTHORS   Padgett,L.R., Shinkle,M.R., Rosario,S., Stewart,T.M., Foley,J.R.,
            Casero,R.A. Jr., Park,M.H., Chung,W.K. and Mastracci,T.L.
  TITLE     Deoxyhypusine synthase mutations alter the post-translational
            modification of eukaryotic initiation factor 5A resulting in
            impaired human and mouse neural homeostasis
  JOURNAL   HGG Adv 4 (3), 100206 (2023)
   PUBMED   37333770
  REMARK    GeneRIF: Deoxyhypusine synthase mutations alter the
            post-translational modification of eukaryotic initiation factor 5A
            resulting in impaired human and mouse neural homeostasis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1361)
  AUTHORS   Coni,S., Bordone,R., Ivy,D.M., Yurtsever,Z.N., Di Magno,L.,
            D'Amico,R., Cesaro,B., Fatica,A., Belardinilli,F., Bufalieri,F.,
            Maroder,M., De Smaele,E., Di Marcotullio,L., Giannini,G.,
            Agostinelli,E. and Canettieri,G.
  TITLE     Combined inhibition of polyamine metabolism and eIF5A hypusination
            suppresses colorectal cancer growth through a converging effect on
            MYC translation
  JOURNAL   Cancer Lett 559, 216120 (2023)
   PUBMED   36893894
  REMARK    GeneRIF: Combined inhibition of polyamine metabolism and eIF5A
            hypusination suppresses colorectal cancer growth through a
            converging effect on MYC translation.
REFERENCE   5  (bases 1 to 1361)
  AUTHORS   Schultz,C.R., Sheldon,R.D., Xie,H., Demireva,E.Y., Uhl,K.L.,
            Agnew,D.W., Geerts,D. and Bachmann,A.S.
  TITLE     New K50R mutant mouse models reveal impaired hypusination of eif5a2
            with alterations in cell metabolite landscape
  JOURNAL   Biol Open 12 (3) (2023)
   PUBMED   36848144
REFERENCE   6  (bases 1 to 1361)
  AUTHORS   Nishimura,K., Murozumi,K., Shirahata,A., Park,M.H., Kashiwagi,K.
            and Igarashi,K.
  TITLE     Independent roles of eIF5A and polyamines in cell proliferation
  JOURNAL   Biochem J 385 (Pt 3), 779-785 (2005)
   PUBMED   15377278
  REMARK    GeneRIF: a decrease in either active eIF5A or polyamines inhibits
            cell growth, indicating that eIF5A and polyamines are independently
            involved in cell growth
REFERENCE   7  (bases 1 to 1361)
  AUTHORS   Jin,B.F., He,K., Wang,H.X., Wang,J., Zhou,T., Lan,Y., Hu,M.R.,
            Wei,K.H., Yang,S.C., Shen,B.F. and Zhang,X.M.
  TITLE     Proteomic analysis of ubiquitin-proteasome effects: insight into
            the function of eukaryotic initiation factor 5A
  JOURNAL   Oncogene 22 (31), 4819-4830 (2003)
   PUBMED   12894223
REFERENCE   8  (bases 1 to 1361)
  AUTHORS   Nishimura,K., Ohki,Y., Fukuchi-Shimogori,T., Sakata,K., Saiga,K.,
            Beppu,T., Shirahata,A., Kashiwagi,K. and Igarashi,K.
  TITLE     Inhibition of cell growth through inactivation of eukaryotic
            translation initiation factor 5A (eIF5A) by deoxyspergualin
  JOURNAL   Biochem J 363 (Pt 3), 761-768 (2002)
   PUBMED   11964177
REFERENCE   9  (bases 1 to 1361)
  AUTHORS   Jao,D.L. and Yu Chen,K.
  TITLE     Subcellular localization of the hypusine-containing eukaryotic
            initiation factor 5A by immunofluorescent staining and green
            fluorescent protein tagging
  JOURNAL   J Cell Biochem 86 (3), 590-600 (2002)
   PUBMED   12210765
REFERENCE   10 (bases 1 to 1361)
  AUTHORS   Jenkins,Z.A., Haag,P.G. and Johansson,H.E.
  TITLE     Human eIF5A2 on chromosome 3q25-q27 is a phylogenetically conserved
            vertebrate variant of eukaryotic translation initiation factor 5A
            with tissue-specific expression
  JOURNAL   Genomics 71 (1), 101-109 (2001)
   PUBMED   11161802
  REMARK    GeneRIF: Eukaryotic initiation factor 5A (eIF5A) (eIF-4D, eIF-5A)
            stimulates ribosomal peptidyltransferase activity, transport of
            HIV-1-mRNAs and binds exportins 1 and 4. Contains hypusine at lys
            50. Mouse EIF5A1 and EIF5A2 encode two isoforms: eIF5AI and
            eIF5AII.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL596185.12, AK011306.1,
            AK010183.1, AK218644.1 and AI850684.1.
            
            On Nov 4, 2009 this sequence version replaced NM_181582.3.
            
            Summary: This gene encodes an elongation initiation factor, which
            participates in protein synthesis. The encoded protein also plays
            roles in mRNA metabolism, cell proliferation, and cell cycle
            control. This protein contains a modified lysine residue called
            hypusine, which appears to be necessary for its function.
            Alternatively spliced transcript variants have been described.
            Related pseudogenes exist on chromosomes 2, 5, and 19. [provided by
            RefSeq, Oct 2009].
            
            Transcript Variant: This variant (5, also referred to as variant B)
            differs in the 5' UTR compared to variant 1. Variants 1-9 encode
            the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY129325.1, BC024899.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                AL596185.12        32987-33031         c
            46-149              AK011306.1         3-106
            150-150             AK010183.1         51-51
            151-971             AK011306.1         107-927
            972-1202            AK218644.1         144-374
            1203-1361           AI850684.1         6-164               c
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.92 cM"
     gene            1..1361
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /note="eukaryotic translation initiation factor 5A"
                     /db_xref="GeneID:276770"
                     /db_xref="MGI:MGI:106248"
     exon            1..224
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /inference="alignment:Splign:2.1.0"
     exon            225..410
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..710
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 5A-1"
                     /protein_id="NP_853613.1"
                     /db_xref="CCDS:CCDS24923.1"
                     /db_xref="GeneID:276770"
                     /db_xref="MGI:MGI:106248"
                     /translation="MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMS
                     TSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLL
                     QDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK"
     exon            411..515
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /inference="alignment:Splign:2.1.0"
     exon            516..647
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /inference="alignment:Splign:2.1.0"
     exon            648..721
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /inference="alignment:Splign:2.1.0"
     exon            722..1351
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1319..1324
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /note="hexamer: AATAAA"
     polyA_site      1351
                     /gene="Eif5a"
                     /gene_synonym="D19Wsu54e; eIF-4D; eIF-5A; eIF-5A-1;
                     eIF-5A1; Eif4d; Eif5a1"
                     /note="major polyA site"
ORIGIN      
        1 gcgggggtgg aaggtggtgg agctacctag cacgcttgcg cggctgtcat agggctgctt
       61 ggttggtcag tggggagtcg gcgcctgcgt actaagaccc gtgtgcagca gcggcggcgg
      121 cgctagaggc ggcggcggcg gcggcggcag cacagtagcg ggttcggagg cagcagttgg
      181 gctcgcggcg agcggaccag gtcaagtcag tgcgttcgcg cgagttggaa tcgaagcctc
      241 ttaaaatggc agatgatttg gacttcgaga caggagatgc aggggcctca gccaccttcc
      301 caatgcagtg ctcagcatta cgtaagaatg gttttgtggt gctcaaaggc cggccatgta
      361 agatcgtcga gatgtctact tcgaagactg gcaagcatgg ccatgccaag gtccatctgg
      421 ttggcattga catttttact gggaagaaat atgaagatat ctgcccgtca actcataata
      481 tggatgtccc caacatcaaa cggaatgact tccagctgat tggcatccag gatgggtacc
      541 tatccctgct ccaggacagt ggggaggtac gagaggacct tcgtctgcct gaaggagacc
      601 ttggcaagga gattgagcag aagtatgact gtggagaaga gatcctgatc acagtgctgt
      661 ctgccatgac agaggaggca gctgttgcaa tcaaggccat ggcaaaataa ccggcttcca
      721 gggtggcggt ggtggcagca gtgatccatg agcctacaga ggcccctccc ccagccaggt
      781 tctgctctgg ctgggccctt ggctggactc ctattcaaat ttatttgacg ttttattttg
      841 gttttcctca ccccctcaaa ctgtcgggga gaccctgccc ttcacctagc tcccttggcc
      901 aggaatgagg gagccatggc cttggtgaaa ctccctgcct cttctctcgc agccctgatg
      961 ggggaaaggg agttgggtac tgcttgtggt ttaggttccc ctctcctttt tttcttttta
     1021 attcaatttg gaatcagaaa gctggattct ggtaaatggt cttgtgccct ttatcctact
     1081 catccccgat ctggtcccct gttctccata gtccttcacc cccaagcacc actgtacaga
     1141 ctggggacca gccccttccc tgcctgtgtc tcttcccaaa cccctgtagg ggtggtgaga
     1201 agaggagggg gggaggggac acgatccctc ctcaggcatc tgggaaggcc ttgcccccac
     1261 gggctttacc ctttcctgtg ggctctctcc ctgacacatt tgttaaaaat caaacctgaa
     1321 taaaactaca agtttaatat gaagccccca aaaaaaaaaa a
//
