LOCUS       NM_001256195            6241 bp    mRNA    linear   ROD 17-JUL-2024
DEFINITION  Mus musculus eukaryotic translation initiation factor 4 gamma, 3
            (Eif4g3), transcript variant 2, mRNA.
ACCESSION   NM_001256195
VERSION     NM_001256195.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Kim,S.H., Choi,J.H., Marsal-Garcia,L., Amiri,M., Yanagiya,A. and
            Sonenberg,N.
  TITLE     The mRNA translation initiation factor eIF4G1 controls
            mitochondrial oxidative phosphorylation, axonal morphogenesis, and
            memory
  JOURNAL   Proc Natl Acad Sci U S A 120 (25), e2300008120 (2023)
   PUBMED   37307456
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Kang,J.Y., Wen,Z., Pan,D., Zhang,Y., Li,Q., Zhong,A., Yu,X.,
            Wu,Y.C., Chen,Y., Zhang,X., Kou,P.C., Geng,J., Wang,Y.Y., Hua,M.M.,
            Zong,R., Li,B., Shi,H.J., Li,D., Fu,X.D., Li,J., Nelson,D.L.,
            Guo,X., Zhou,Y., Gou,L.T., Huang,Y. and Liu,M.F.
  TITLE     LLPS of FXR1 drives spermiogenesis by activating translation of
            stored mRNAs
  JOURNAL   Science 377 (6607), eabj6647 (2022)
   PUBMED   35951695
REFERENCE   3  (bases 1 to 6241)
  AUTHORS   Senechal,P., Robert,F., Cencic,R., Yanagiya,A., Chu,J.,
            Sonenberg,N., Paquet,M. and Pelletier,J.
  TITLE     Assessing eukaryotic initiation factor 4F subunit essentiality by
            CRISPR-induced gene ablation in the mouse
  JOURNAL   Cell Mol Life Sci 78 (19-20), 6709-6719 (2021)
   PUBMED   34559254
REFERENCE   4  (bases 1 to 6241)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6241)
  AUTHORS   Gonatopoulos-Pournatzis,T., Niibori,R., Salter,E.W.,
            Weatheritt,R.J., Tsang,B., Farhangmehr,S., Liang,X.,
            Braunschweig,U., Roth,J., Zhang,S., Henderson,T., Sharma,E.,
            Quesnel-Vallieres,M., Permanyer,J., Maier,S., Georgiou,J.,
            Irimia,M., Sonenberg,N., Forman-Kay,J.D., Gingras,A.C.,
            Collingridge,G.L., Woodin,M.A., Cordes,S.P. and Blencowe,B.J.
  TITLE     Autism-Misregulated eIF4G Microexons Control Synaptic Translation
            and Higher Order Cognitive Functions
  JOURNAL   Mol Cell 77 (6), 1176-1192 (2020)
   PUBMED   31999954
REFERENCE   6  (bases 1 to 6241)
  AUTHORS   Unhavaithaya,Y., Hao,Y., Beyret,E., Yin,H., Kuramochi-Miyagawa,S.,
            Nakano,T. and Lin,H.
  TITLE     MILI, a PIWI-interacting RNA-binding protein, is required for germ
            line stem cell self-renewal and appears to positively regulate
            translation
  JOURNAL   J Biol Chem 284 (10), 6507-6519 (2009)
   PUBMED   19114715
REFERENCE   7  (bases 1 to 6241)
  AUTHORS   Sonenberg,N. and Hinnebusch,A.G.
  TITLE     Regulation of translation initiation in eukaryotes: mechanisms and
            biological targets
  JOURNAL   Cell 136 (4), 731-745 (2009)
   PUBMED   19239892
  REMARK    Review article
REFERENCE   8  (bases 1 to 6241)
  AUTHORS   Munton,R.P., Tweedie-Cullen,R., Livingstone-Zatchej,M.,
            Weinandy,F., Waidelich,M., Longo,D., Gehrig,P., Potthast,F.,
            Rutishauser,D., Gerrits,B., Panse,C., Schlapbach,R. and Mansuy,I.M.
  TITLE     Qualitative and quantitative analyses of protein phosphorylation in
            naive and stimulated mouse synaptosomal preparations
  JOURNAL   Mol Cell Proteomics 6 (2), 283-293 (2007)
   PUBMED   17114649
REFERENCE   9  (bases 1 to 6241)
  AUTHORS   Easterday,M.C., Dougherty,J.D., Jackson,R.L., Ou,J., Nakano,I.,
            Paucar,A.A., Roobini,B., Dianati,M., Irvin,D.K., Weissman,I.L.,
            Terskikh,A.V., Geschwind,D.H. and Kornblum,H.I.
  TITLE     Neural progenitor genes. Germinal zone expression and analysis of
            genetic overlap in stem cell populations
  JOURNAL   Dev Biol 264 (2), 309-322 (2003)
   PUBMED   14651920
REFERENCE   10 (bases 1 to 6241)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK019693.1, BC079885.1, CX566741.1 and BE852849.1.
            
            Transcript Variant: This variant (2) contains an alternate exon in
            the 5' UTR and lacks an alternate, in-frame, exon in the 5' coding
            region, compared to variant 1. The resulting protein (isoform 2) is
            shorter when it is compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079885.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AK019693.1         2-47
            47-5639             BC079885.1         1-5593
            5640-6217           CX566741.1         78-655
            6218-6241           BE852849.1         1-24                c
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4"
     gene            1..6241
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="eukaryotic translation initiation factor 4 gamma,
                     3"
                     /db_xref="GeneID:230861"
                     /db_xref="MGI:MGI:1923935"
     exon            1..172
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            173..335
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            336..411
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            412..540
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            541..636
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     CDS             607..5310
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     eIF-4-gamma 3; eIF-4-gamma II"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 4 gamma
                     3 isoform 2"
                     /protein_id="NP_001243124.1"
                     /db_xref="GeneID:230861"
                     /db_xref="MGI:MGI:1923935"
                     /translation="MNSQPQARSPGGFRPIQFFQRPQIQPPRAAIPNSSPSIRPGVQT
                     PTAVYQANQHIMMVNHLPMPYPVTQGHQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPF
                     YPGPGPGDFANAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKD
                     ITEEIMSGGGSRNPTPPIGRPASTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVT
                     AASDQKQEEKPKPDPVFQSPSTVLRLVLSGEKKEQAGQMPETAAGEPTPEPPRTSSPT
                     SLPPLARSSLPSPMSAALSSQPLFTAEDKCELPSSKEEDAPPVPSPTSCTAASGPSLT
                     DNSDICKKPCSVAPHDSQLISSTILINEMNGVGEKLSAKENTVGMLRQEVLPLTLELE
                     ILEHPQEELKVECTPTPIAPSMLPAFSPAPPTPPTSPPCPPVVLSAAIARSPAVATEV
                     QRVADEGESLRTCLSKDAKEMQDKAESESDGQAEETADPQSLHSGRSPAPVQTATTAP
                     KSWKKTKEQTRTPDEVLEAEAEPKAEEELAVDSVLEPEQEKMSQGFPSERDPSALKRG
                     KAEEGNGEEAEPVRNGAESASEGEGGDGNSGSADSSADGLTFPFKAESWKPADTEGKK
                     QYDREFLLDIQFMPACIQKPEGLPPISDVVLDKINQPRLSMRTLDPRILPRGPDFTPA
                     FADFPRQTPGGRGVPLLNVGPRRSQPGQRREPRKIITVSVKEDVHLRKAENAWKPSQK
                     RDSHADDPESIKTQELFRKVRSILNKLTPQMFNQLMKQVSALTVDTEERLKGVIDLVF
                     EKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDV
                     FEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHD
                     CVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIR
                     FMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQ
                     RVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKAS
                     ESAPSGSPSATPLEFDSRRALTSRGSMGREKSDKPIPAGTARPNTFLRGSSKDLLDNQ
                     SQEEQRREMLETVKQLTGGLDAERASTEADRSKTRELAKSEMCAVPAPDKPALSEEEV
                     ERKSKSIIDEFLHINDFKEATQCIEELSAQGPLHVFVKVGVEFTLERSQITRDHMGHL
                     LYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKGGGISMREL
                     IVEFSKPLLPVGRAGVLLSEILHLLCRQMSHKKVGALWREADLSWKDFLPEGEDVHHF
                     LLEQKLDFTESEGPCSSEALSKKELSAEELSQRLEKLIMEEKADDERIFDWVEANLDE
                     SQMSSPTFLRALMTAVCKAAIIADCSTFRVDTAVIKQRVPILLKYLDSDTEKELQALY
                     ALQASIVKLDQPANLLRMFFDCLYDEEVISEDAFYKWESSKDPAEQAGKGVALKSVTA
                     FFTWLREAEEESEDN"
     exon            637..657
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            658..837
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            838..952
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1050
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1173
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1282
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..2107
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2196
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2443
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2577
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2697
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2868
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            2869..3066
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3067..3213
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3214..3450
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..3555
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3556..3688
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3689..3783
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3784..3877
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3878..3938
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            3939..4156
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4157..4272
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4273..4445
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4446..4571
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4572..4653
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4752
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4753..4899
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4900..4981
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            4982..5128
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     exon            5129..6235
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5318..5323
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="hexamer: AATACA"
     polyA_site      5337
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="major polyA site"
     regulatory      5523..5528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="hexamer: AATACA"
     polyA_site      5548
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="major polyA site"
     regulatory      6191..6196
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="hexamer: AATAAA"
     polyA_site      6218
                     /gene="Eif4g3"
                     /gene_synonym="1500002J22Rik; 4930523M17Rik; eIF4GII;
                     G1-419-52; Gm32394; repro8"
                     /note="major polyA site"
ORIGIN      
        1 gtcctgccgg actgctggag gcggccgcag cgccatgttg gatgctctgc tcgttgagtg
       61 aagaaaatcc gtccgcatcg cccgagcccc gctaccgaga agggcgccgc ttcccccggg
      121 gagggggaca gagacccccc gccgccggcc catgaggata ttgccgtgaa aggctcagcg
      181 gctgcagcag gaaccggacc cggcaccgga gcggcggcgg cggcggcggt accgcctcct
      241 catccggcgg cagcggcggc ggcggcggca gtagcggcag cggcggcccc tcctcacccg
      301 aacatcaggg ccctccagac cccggcgccc caacagattc cacgaggacc ggtgcagcag
      361 cctttggagg atcggctctt cccacctacg gtctctgcag tctatagcac ggtaacacaa
      421 gtagcgagac agccaggacc ccccacccct gccccttact cagcacatga aataagcaag
      481 gggctcccca gtcttgcggc cacgcccccg ggacatgcat cgtcccctgg actctctcaa
      541 accccttatc cctctggaca gaatgcaggt ccagccacat tggtctatcc tcaagcccct
      601 cagacaatga actcacaacc tcaggcccgg tctccgggag gattcagacc catccagttt
      661 ttccagaggc ctcaaatcca gcctccccga gctgccatcc caaacagcag tccttccatc
      721 cgtcccggtg tgcagacccc caccgccgtg tatcaggcca accagcacat catgatggtt
      781 aaccatctgc ccatgccgta ccccgtgacc caggggcatc agtactgcat accacagtac
      841 cgccacagcg gccctccgta cgtggggccc ccacagcagt atccagttca gccgcctggg
      901 ccggggcctt tttatcctgg accaggacct ggggacttcg ctaatgctta tggaacgcct
      961 ttttacccaa gtcagccagt gtatcagtca gcacctatca tagtgcctac gcagcagcag
     1021 ccccctccag ccaagagaga gaaaaaaact ataagaattc gagatccaaa tcagggaggt
     1081 aaagacataa cagaggagat tatgtctgga ggtggcagca gaaatcccac tccacctata
     1141 ggaagacccg catccactcc cactcctcct cagcagctgc ccagccaggt ccctgagcac
     1201 agccctgtgg tatatgggac tgtggagagc gctcatcttg ctgccagcac ccctgtcact
     1261 gcagctagcg accagaagca agaagaaaag ccaaaaccag atccagtgtt ccagtctcct
     1321 tcgacggtcc ttaggctcgt actcagtggg gagaagaaag agcaagcagg ccagatgcct
     1381 gagactgctg caggagagcc caccccagag cctcctcgga cttcatcacc caccagcctt
     1441 cctccacttg cccggagttc acttccttcc cccatgtccg ctgcccttag cagccagcca
     1501 ctgttcactg ctgaggacaa gtgtgaactt ccttcctcca aagaagaaga tgcacctcct
     1561 gtccccagcc ccacgtcttg cacagcagcc tcaggccctt cactaacaga taacagtgat
     1621 atatgcaaga aaccctgtag cgtagcgcct catgatagtc agctaatttc tagtactatc
     1681 ctaattaatg aaatgaatgg agttggagaa aaattatcag ccaaggagaa cactgtgggc
     1741 atgctgagac aggaagtgct gccattgact ctcgagttag agattctgga acatccacag
     1801 gaagaattga aagtggaatg tacgccgact cccattgccc cgtccatgct gcctgccttc
     1861 tccccagctc ctccgactcc ccccacttct cctccttgtc ctccggttgt cctctctgcc
     1921 gccattgctc gttctcccgc tgttgccact gaggtccaga gagtcgccga cgagggagaa
     1981 agcctgagaa cttgccttag caaggatgca aaggagatgc aggacaaagc agagtcagaa
     2041 tcagatgggc aagcagaaga gactgcggat ccccagagcc tacattcagg aaggagcccc
     2101 gccccagtgc aaacagctac cactgcgcca aagtcctgga agaaaacaaa agagcagacc
     2161 cgaaccccag atgaagtgtt agaagcagag gcagagccta aagccgaaga agagcttgca
     2221 gttgacagtg tccttgagcc tgagcaagag aagatgagcc aagggtttcc gtctgagaga
     2281 gacccctctg ccctgaagag agggaaagct gaggaaggaa acggagaaga ggctgagcct
     2341 gtgcgaaatg gcgctgagag tgcttctgag ggggaagggg gagatggcaa ctcaggctct
     2401 gctgacagct ctgctgatgg gctcaccttc ccgtttaaag cagagtcctg gaagcctgca
     2461 gatacagaag gcaagaagca gtatgaccgg gagttcctac tggacatcca gtttatgccc
     2521 gcctgtatcc aaaaaccaga aggcctgcct cccatcagcg acgtggtcct tgacaagatc
     2581 aaccagccca gattgtcaat gcggaccctg gatcctcgca ttttgcctcg aggacctgac
     2641 ttcaccccag cctttgcaga ctttccaagg caaacgcctg gtggaagagg tgtgcctctg
     2701 ttgaatgttg gacctcggag gtctcaacct ggccagagaa gggagcccag aaagataatc
     2761 actgtctccg taaaagaaga tgtgcacttg agaaaggcgg agaatgcctg gaagccgagc
     2821 cagaaacggg acagccacgc cgatgacccc gaaagcatta aaactcagga acttttcaga
     2881 aaagttcgaa gtatcttgaa taaactgaca ccacagatgt tcaaccagct gatgaagcaa
     2941 gtgtcggctc ttacggtgga cacggaggag cggctaaaag gggtcatcga ccttgtcttt
     3001 gagaaggcta tcgatgagcc cagcttctct gtggcgtacg caaacatgtg tcggtgccta
     3061 gtaacgctga aggtgcccat ggcagacaag cctggcaaca cagtgaattt ccggaagctg
     3121 ctgcttaacc gctgccagaa ggagttcgag aaagacaaag cagatgatga cgtctttgag
     3181 aagaagcaga aagaactgga agctgccagc gctccagagg agaggacaag gcttcacgac
     3241 gaactggaag aagccaagga caaagcccgg cggagatcca tcggcaacat caagttcatt
     3301 ggagaactct ttaaactgaa aatgctgacg gaggccatca tgcatgactg tgtggtgaag
     3361 ctcctgaaga accacgatga agagtccctg gagtgcctgt gtcgcctgct caccaccatc
     3421 ggcaaagacc tggactttga gaaagcaaag ccacgcatgg accagtactt taaccagatg
     3481 gagaaaatcg tcaaagaaag aaaaacttcc tccagaattc ggttcatgct tcaggatgta
     3541 atcgacctca gactgtgtaa ttgggtgtcc cgaagagcag atcaggggcc aaaaactatc
     3601 gagcagattc acaaagaggc caaaatagaa gaacaagagg agcagaggaa agtccagcag
     3661 ctgatgacca aggagaagag gagaccaggc gtccaaagag tggatgaagg tggatggaat
     3721 actgtacaag gggccaagaa cagtcgtgta ctggacccct ccaaattttt gaaaattact
     3781 aaacccacca ttgatgagaa gattcagctg gttcctaagg cacagctggg cagctggggc
     3841 aaaggcagca gcgggggagc aaaggcaagt gagtccgcac cttcagggtc cccgtcggcc
     3901 acacccttag agtttgattc ccgaagggcg ttaaccagtc gtgggagcat gggcagggag
     3961 aagagcgaca agccaattcc agctggaaca gcccgtccca acacgttcct gaggggcagc
     4021 agcaaagacc tgctggacaa ccagtcccag gaagagcagc gcagagagat gttggagacc
     4081 gtgaaacagc tgacaggagg cctggatgct gagcgggcca gcactgaggc cgaccgaagc
     4141 aaaacgaggg agctagcaaa atcggaaatg tgtgcagtgc cagcccctga caagcctgca
     4201 ctgtcagaag aggaagtgga gaggaagtcc aagtctatca ttgacgaatt cttacacatc
     4261 aatgacttta aggaggccac gcagtgcata gaggagctga gcgcccaggg cccactgcat
     4321 gtgttcgtga aggtgggtgt ggagttcacc ctggaacgga gccagatcac cagggaccac
     4381 atgggccact tactgtatca gctggtgcag tcagaaaaac tcagcaagca ggactttttc
     4441 aaaggttttt ctgaaacctt ggagttggca gatgacatgg ccattgatat tccccatatt
     4501 tggttgtacc tggctgaact ggtcaccccc atgttaaaag gaggggggat ttccatgaga
     4561 gaacttattg tggaattcag caagccatta cttcctgttg gcagagctgg ggtcctgctt
     4621 tctgaaatcc tacaccttct atgcagacaa atgagccata agaaagtagg cgccctgtgg
     4681 agggaggctg acctcagctg gaaggacttt ttaccggaag gggaagatgt ccatcatttt
     4741 ctcttggagc agaagttgga cttcacggaa tccgaagggc cctgctcctc tgaggcactg
     4801 tcaaagaagg agctgtctgc cgaggagctg tctcagcgcc tggaaaagct catcatggag
     4861 gagaaagcgg atgacgagcg gatctttgac tgggtggagg ctaatctcga cgaaagccag
     4921 atgagttcgc ctacattcct tagagcttta atgacagccg tttgcaaagc agctatcata
     4981 gctgactgtt ctaccttcag agtggacact gctgtgatca agcagagagt gccgatctta
     5041 ctcaagtacc tagactcaga cacggagaag gaactacaag cactttatgc actacaagca
     5101 tccatagtaa aacttgacca acctgccaac ttgctccgga tgttttttga ttgcctgtat
     5161 gacgaggagg tgatatcaga ggatgccttc tacaaatggg agagcagcaa ggaccccgct
     5221 gagcaagctg ggaagggcgt ggccctcaag tctgtcacgg cctttttcac gtggcttcga
     5281 gaagcagaag aggagtccga ggataactaa tacttcaaat acacaaacga aacaaaagaa
     5341 acaatttaag tattttttta aaaagtttca cgtcttcgcc aatcacagtg cagcaaggcc
     5401 aactctcgca gaaaccccac gtgtgcgcga gtgggagagg ggacaggaat caggtgatca
     5461 tggaggataa aggtcctgga acaaggaaac agcaaacccg agggcgggcg ctccaaaacc
     5521 aaaatacatg tactatttat agaaaatatt ttctgtttta atcttttctt tttaaacgag
     5581 gactcatact ttaaaaaaaa aaataaaaaa aaaaacacat ttagcaaaaa aaaaaaaaag
     5641 aaaaaagaaa aaaaagaaaa agaaaaaaaa aacagttgaa ctttaattta ttttaaggac
     5701 tgcaaatgcc agtgtaattt ttttaatttg cagtttctgt aaacaacttg tataatagaa
     5761 aagcagagaa ataaatttcc cttcccttca gatgcaccgc acatttgtgt taaggcatag
     5821 ctgttagtcc cgatttgaga aggtttgggg tgaacgaggt aagaaagatc ttttttccct
     5881 tccccggccc ttttcttgct ccactcccag ccctatcttc agatctttct gcctctcagc
     5941 ttgcttcaga aaatttaaaa atcacactag taatcaaaac atacataacc ttggaacaga
     6001 aggaagtact gtggaccaga aaaatccaag aattgttcaa aaaaagtgct accctggaaa
     6061 atacccttaa taccgttgaa atcgttagag taactttaag gcttgtaaat acatagaaca
     6121 aatatttaaa aacataaaaa aggaattgac tcaatactat ttcttttcac tttcaaaata
     6181 taaggaacaa aataaagacg agcattgcaa gtttaaaata aagacttctc cttttaaaaa
     6241 a
//
