LOCUS       NM_001285942             658 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus eukaryotic translation initiation factor 3, subunit K
            (Eif3k), transcript variant 2, mRNA.
ACCESSION   NM_001285942
VERSION     NM_001285942.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 658)
  AUTHORS   Fujii,K., Zhulyn,O., Byeon,G.W., Genuth,N.R., Kerr,C.H., Walsh,E.M.
            and Barna,M.
  TITLE     Controlling tissue patterning by translational regulation of
            signaling transcripts through the core translation factor eIF3c
  JOURNAL   Dev Cell 56 (21), 2928-2937 (2021)
   PUBMED   34752747
REFERENCE   3  (bases 1 to 658)
  AUTHORS   Zhao,Y., Zheng,D. and Cvekl,A.
  TITLE     A comprehensive spatial-temporal transcriptomic analysis of
            differentiating nascent mouse lens epithelial and fiber cells
  JOURNAL   Exp Eye Res 175, 56-72 (2018)
   PUBMED   29883638
REFERENCE   4  (bases 1 to 658)
  AUTHORS   Lin,Y.M., Chen,Y.R., Lin,J.R., Wang,W.J., Inoko,A., Inagaki,M.,
            Wu,Y.C. and Chen,R.H.
  TITLE     eIF3k regulates apoptosis in epithelial cells by releasing caspase
            3 from keratin-containing inclusions
  JOURNAL   J Cell Sci 121 (Pt 14), 2382-2393 (2008)
   PUBMED   18577580
REFERENCE   5  (bases 1 to 658)
  AUTHORS   Masutani,M., Sonenberg,N., Yokoyama,S. and Imataka,H.
  TITLE     Reconstitution reveals the functional core of mammalian eIF3
  JOURNAL   EMBO J 26 (14), 3373-3383 (2007)
   PUBMED   17581632
REFERENCE   6  (bases 1 to 658)
  AUTHORS   Wei,Z., Zhang,P., Zhou,Z., Cheng,Z., Wan,M. and Gong,W.
  TITLE     Crystal structure of human eIF3k, the first structure of eIF3
            subunits
  JOURNAL   J Biol Chem 279 (33), 34983-34990 (2004)
   PUBMED   15180986
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166357.3.
            
            On Dec 21, 2021 this sequence version replaced NM_001285942.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027638.1, ERR2844020.84851.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AC166357.3         2316-2440
            126-246             AC166357.3         6124-6244
            247-321             AC166357.3         6839-6913
            322-388             AC166357.3         9445-9511
            389-466             AC166357.3         9888-9965
            467-592             AC166357.3         11457-11582
            593-658             AC166357.3         12676-12741
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 16.94 cM"
     gene            1..658
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /note="eukaryotic translation initiation factor 3, subunit
                     K"
                     /db_xref="GeneID:73830"
                     /db_xref="MGI:MGI:1921080"
     exon            1..125
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..624
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     eukaryotic translation initiation factor 3, subunit 12;
                     eIF-3 p25"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 3
                     subunit K isoform 2"
                     /protein_id="NP_001272871.1"
                     /db_xref="CCDS:CCDS85254.1"
                     /db_xref="GeneID:73830"
                     /db_xref="MGI:MGI:1921080"
                     /translation="MAMFEQMRANVGKLLKGIDRYQFNPAFFQTTVTAQILLKALTNL
                     PHTDFTLCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFE
                     DSVRKFICHVVGITYQHIDRWLLAEMLGDLTDNQLKVWMSKYGWSADESGQVFICSQE
                     ESIKPKNIVEKIDFDSVSSIMASSQ"
     exon            126..246
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /inference="alignment:Splign:2.1.0"
     exon            247..321
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /inference="alignment:Splign:2.1.0"
     exon            322..388
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /inference="alignment:Splign:2.1.0"
     exon            389..466
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /inference="alignment:Splign:2.1.0"
     exon            467..592
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /inference="alignment:Splign:2.1.0"
     exon            593..658
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      638..643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /note="hexamer: AATAAA"
     polyA_site      658
                     /gene="Eif3k"
                     /gene_synonym="1200009C21Rik; Eif3s12"
                     /note="major polyA site"
ORIGIN      
        1 cctctagtgc taagcaccgt gcctggtgtt cgactccagc tgaggctgcc gaaggcaaca
       61 gtgatcatgg cgatgtttga gcagatgaga gcgaacgtgg gcaagttgct caagggtatc
      121 gacaggtacc agttcaaccc agccttcttc cagaccacag tcactgccca gattctgctg
      181 aaagccctca ccaacctgcc ccacaccgac ttcactctgt gtaaatgtat gatcgaccag
      241 gcacatcaag aagagcggcc catccggcag atcttgtacc tcggggacct gctggagacc
      301 tgccactttc aagccttctg gcaagccctg gatgagaaca tggaccttct ggaaggcata
      361 actggctttg aagactctgt ccgaaaattt atctgccacg tggtgggcat cacgtaccag
      421 cacatcgacc gctggctgct ggccgagatg ctcggagacc tgactgacaa ccagctgaag
      481 gtgtggatga gcaagtacgg ctggagcgct gacgagtcag ggcaggtctt catctgcagc
      541 caggaagaga gcattaagcc caagaacatc gtggagaaga ttgactttga cagtgtgtcc
      601 agcatcatgg cctcctccca gtaagccagt gtgcgttaat aaagatttgt tcactccg
//
