LOCUS       NM_001111277            1571 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus eukaryotic translation initiation factor 2B, subunit 3
            (Eif2b3), transcript variant 1, mRNA.
ACCESSION   NM_001111277
VERSION     NM_001111277.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   3  (bases 1 to 1571)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 1571)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   5  (bases 1 to 1571)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
REFERENCE   6  (bases 1 to 1571)
  AUTHORS   Nelson,P.S., Pritchard,C., Abbott,D. and Clegg,N.
  TITLE     The human (PEDB) and mouse (mPEDB) Prostate Expression Databases
  JOURNAL   Nucleic Acids Res 30 (1), 218-220 (2002)
   PUBMED   11752298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ828648.1, BY277686.1, AK145856.1, BC115657.1 and BE952243.1.
            
            On Sep 20, 2017 this sequence version replaced NM_001111277.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660821.127166.1,
                                           SRR1660819.306631.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 BQ828648.1         2-7
            7-30                BY277686.1         1-24
            31-379              AK145856.1         2-350
            380-1520            BC115657.1         48-1188
            1521-1571           BE952243.1         1-51                c
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 53.41 cM"
     gene            1..1571
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /note="eukaryotic translation initiation factor 2B,
                     subunit 3"
                     /db_xref="GeneID:108067"
                     /db_xref="MGI:MGI:1313286"
     exon            1..75
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            76..233
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1444
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="translation initiation factor eIF2B subunit
                     gamma isoform 1"
                     /protein_id="NP_001104747.1"
                     /db_xref="CCDS:CCDS51279.1"
                     /db_xref="GeneID:108067"
                     /db_xref="MGI:MGI:1313286"
                     /translation="MEFQAVVMAVGGGSRMTDLTSSIPKPLLPVGNKPLIWYPLNLLE
                     RVGFEEVIVVTTKDVQKALCAEFKMKMKLDIVCIPDEADMGTADSLRHIYPKLKTDVL
                     VLSCDLITDVALHEVVDLFRAYDASLAMLMRKGQESIEPVPGQKGKKKPVEQRDFIGV
                     DSTGKRLLFMANEADLDEELVIKGSILQKHPRIHFHTGLVDAHLYCLKKYVVDFLMEN
                     RSITSIRSELIPYLVRKQFSSASSQQRQEDKEEDLKKKELKSLDIYSFIKKDDTLTLA
                     PYDACWNAFRGDKWEDLSRSQVRCYVHIMKEGLCSRVSTLGLYMEANRQVPKLLSVLC
                     PEESMIHSSAQIVNKHLIGADSLIGPDTQIGEKSSIKHSVIGSSCVIRDRTSITNCLL
                     MNSVTVEEGSSIHGSVICNNAVIETGAEIKNCLVGSGQRIEAKAKRMNEVIVGNDQLM
                     EI"
     exon            234..379
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            380..539
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            540..651
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            652..741
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            742..869
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            870..1060
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1138
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1287
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1391
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1555
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1531..1536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1555
                     /gene="Eif2b3"
                     /gene_synonym="1190002P15Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcctgcgctc ttggcttgtc agccagaggc ggcgtgggga gcgctgggag aggctgcggc
       61 gtgcggatag ctgagcttta taaaaatgga gtttcaagcg gtggttatgg ccgttggtgg
      121 gggatctcgg atgacagact tgacttccag cattcccaag cctctgcttc cagttggaaa
      181 caaaccttta atttggtacc cgctgaacct gcttgagcgt gttggatttg aagaagtcat
      241 cgtggttaca accaaagatg tccaaaaggc tctctgcgca gaattcaaga tgaagatgaa
      301 gctagatatt gtgtgcattc ctgatgaggc agacatgggg actgcagatt ctctacgaca
      361 catctatcca aagcttaaga ccgatgtgct ggtcctgagc tgcgatctga taacagatgt
      421 ggctttacat gaggttgtgg acctgttcag agcttatgat gcgtcactgg ccatgctaat
      481 gaggaaagga caagagagca tagaaccagt tccaggtcaa aaggggaaaa aaaaaccagt
      541 ggagcagcgt gacttcattg gagtggacag cacaggaaaa aggctgctct ttatggctaa
      601 tgaagcagac ttggatgaag agctggtcat taagggatcc atcctgcaga agcaccctag
      661 gatacatttc cacacaggcc ttgtggatgc ccatctctac tgcctgaaga agtacgttgt
      721 ggatttctta atggagaata ggtcaatcac ttctatccga agcgaactga ttccatacct
      781 agtaagaaaa cagttttcct cagcttcctc acagcaaagg caggaagaca aagaagagga
      841 tctaaagaaa aaggagctga agtctttaga tatttacagt tttataaaaa aagacgacac
      901 tctgaccttg gctccctatg atgcctgctg gaatgctttt cgaggagaca aatgggaaga
      961 tttgtctaga tcacaggtcc gctgctatgt ccacatcatg aaagaaggac tttgctctcg
     1021 agtaagcaca ctgggactct acatggaagc caacagacag gtccccaaat tgctatctgt
     1081 tctttgtccg gaggaatcaa tgatccattc atcagcccag attgtcaaca aacacctgat
     1141 tggagctgat agcctcatcg ggccagatac acaaattgga gagaagtcat ctattaagca
     1201 ctctgtcatt ggctcatcct gtgtcatacg agacagaacg tctattacca attgcctcct
     1261 catgaactct gtcaccgtgg aagaagggag cagcatccac ggcagtgtca tctgcaacaa
     1321 cgcagtgatc gagacgggcg cagagatcaa gaactgtttg gttggaagtg gtcagaggat
     1381 tgaagccaaa gctaaacgaa tgaacgaggt gatcgtgggg aatgaccagc tcatggaaat
     1441 ctgaaccctg aacaagcccg accccttcct ttgggcctca aactacagat gctgcctggc
     1501 ccacctgttt tgactcttta tttatttttc aataaagaag aatttccagg ctgtaaaaaa
     1561 aaaaaaaaaa a
//
