LOCUS       NM_010121               4519 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus eukaryotic translation initiation factor 2 alpha
            kinase 3 (Eif2ak3), transcript variant 1, mRNA.
ACCESSION   NM_010121 XM_912971
VERSION     NM_010121.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Shin,Y.C., Latorre-Muro,P., Djurabekova,A., Zdorevskyi,O.,
            Bennett,C.F., Burger,N., Song,K., Xu,C., Paulo,J.A., Gygi,S.P.,
            Sharma,V., Liao,M. and Puigserver,P.
  TITLE     Structural basis of respiratory complex adaptation to cold
            temperatures
  JOURNAL   Cell 187 (23), 6584-6598 (2024)
   PUBMED   39395414
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Song,C., Chen,Q., Xu,J., He,K., Guo,Q., Teng,X., Xue,H., Xiao,L.,
            Tian,D., Jin,S., An,C. and Wu,Y.
  TITLE     H2S alleviated sepsis-induced acute kidney injury by inhibiting
            PERK/Bax-Bcl2 pathway
  JOURNAL   Nitric Oxide 152, 11-18 (2024)
   PUBMED   39271041
  REMARK    GeneRIF: H2S alleviated sepsis-induced acute kidney injury by
            inhibiting PERK/Bax-Bcl2 pathway.
REFERENCE   3  (bases 1 to 4519)
  AUTHORS   Xu,L., Tan,C., Barr,J., Talaba,N., Verheyden,J., Chin,J.S.,
            Gaboyan,S., Kasaraneni,N., Elgamal,R.M., Gaulton,K.J., Lin,G.,
            Afshar,K., Golts,E., Meier,A., Crotty Alexander,L.E., Borok,Z.,
            Shen,Y., Chung,W.K., McCulley,D.J. and Sun,X.
  TITLE     Context-dependent roles of mitochondrial LONP1 in orchestrating the
            balance between airway progenitor versus progeny cells
  JOURNAL   Cell Stem Cell 31 (10), 1465-1483 (2024)
   PUBMED   39181129
REFERENCE   4  (bases 1 to 4519)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4519)
  AUTHORS   De Leo,A., Ugolini,A., Yu,X., Scirocchi,F., Scocozza,D.,
            Peixoto,B., Pace,A., D'Angelo,L., Liu,J.K.C., Etame,A.B.,
            Rughetti,A., Nuti,M., Santoro,A., Vogelbaum,M.A.,
            Conejo-Garcia,J.R., Rodriguez,P.C. and Veglia,F.
  TITLE     Glucose-driven histone lactylation promotes the immunosuppressive
            activity of monocyte-derived macrophages in glioblastoma
  JOURNAL   Immunity 57 (5), 1105-1123 (2024)
   PUBMED   38703775
REFERENCE   6  (bases 1 to 4519)
  AUTHORS   Brewer,J.W. and Diehl,J.A.
  TITLE     PERK mediates cell-cycle exit during the mammalian unfolded protein
            response
  JOURNAL   Proc Natl Acad Sci U S A 97 (23), 12625-12630 (2000)
   PUBMED   11035797
REFERENCE   7  (bases 1 to 4519)
  AUTHORS   Delepine,M., Nicolino,M., Barrett,T., Golamaully,M., Lathrop,G.M.
            and Julier,C.
  TITLE     EIF2AK3, encoding translation initiation factor 2-alpha kinase 3,
            is mutated in patients with Wolcott-Rallison syndrome
  JOURNAL   Nat Genet 25 (4), 406-409 (2000)
   PUBMED   10932183
REFERENCE   8  (bases 1 to 4519)
  AUTHORS   Bertolotti,A., Zhang,Y., Hendershot,L.M., Harding,H.P. and Ron,D.
  TITLE     Dynamic interaction of BiP and ER stress transducers in the
            unfolded-protein response
  JOURNAL   Nat Cell Biol 2 (6), 326-332 (2000)
   PUBMED   10854322
REFERENCE   9  (bases 1 to 4519)
  AUTHORS   Harding,H.P., Zhang,Y., Bertolotti,A., Zeng,H. and Ron,D.
  TITLE     Perk is essential for translational regulation and cell survival
            during the unfolded protein response
  JOURNAL   Mol Cell 5 (5), 897-904 (2000)
   PUBMED   10882126
REFERENCE   10 (bases 1 to 4519)
  AUTHORS   Harding,H.P., Zhang,Y. and Ron,D.
  TITLE     Protein translation and folding are coupled by an
            endoplasmic-reticulum-resident kinase
  JOURNAL   Nature 397 (6716), 271-274 (1999)
   PUBMED   9930704
  REMARK    Erratum:[Nature 1999 Mar 4;398(6722):90]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC054809.1, AC155842.4 and
            BP762515.1.
            
            On Sep 11, 2015 this sequence version replaced NM_010121.2.
            
            Summary: The protein encoded by this gene phosphorylates the alpha
            subunit of eukaryotic translation-initiation factor 2, leading to
            its inactivation, and thus to a rapid reduction of translational
            initiation and repression of global protein synthesis. This protein
            is thought to modulate mitochondrial function. It is a type I
            membrane protein located in the endoplasmic reticulum (ER), where
            it is induced by ER stress caused by malfolded proteins. Mutations
            in a similar gene in human are associated with Wolcott-Rallison
            syndrome. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054809.1, AF076681.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3275              BC054809.1         1-3275
            3276-3276           AC155842.4         39849-39849
            3277-4507           BC054809.1         3277-4507
            4508-4519           BP762515.1         36-47               c
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 32.09 cM"
     gene            1..4519
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /note="eukaryotic translation initiation factor 2 alpha
                     kinase 3"
                     /db_xref="GeneID:13666"
                     /db_xref="MGI:MGI:1341830"
     exon            1..507
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..3556
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; pancreatic eIF2-alpha kinase; PRKR-like
                     endoplasmic reticulum kinase; protein tyrosine kinase
                     EIF2AK3"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase 3 isoform 1 precursor"
                     /protein_id="NP_034251.2"
                     /db_xref="CCDS:CCDS20223.1"
                     /db_xref="GeneID:13666"
                     /db_xref="MGI:MGI:1341830"
                     /translation="MERATRPGPRALLLLLFLLLGCAAGISAVAPARSLLAPASETVF
                     GLGAAAAPTSAARVPAVATAEVTVEDAEALPAAAGEPESRATEPDDDVELRPRGRSLV
                     IISTLDGRIAALDAENDGKKQWDLDVGSGSLVSSSLSKPEVFGNKMIIPSLDGDLFQW
                     DRDRESMEAVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKLRYICSALGCR
                     RWDSDEMEEEEDILLLQRTQKTVRAVGPRSGSEKWNFSVGHFELRYIPDMETRAGFIE
                     STFKPGGNKEDSKIISDVEEQEATMLDTVIKVSVADWKVMAFSRKGGRLEWEYQFCTP
                     IASAWLVRDGKVIPISLFDDTSYTASEEALGDEEDIVEAARGATENSVYLGMYRGQLY
                     LQSSVRVSEKFPTSPKALESVNGENAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCL
                     SNDKYSHEEYSNGALSILQYPYDNGYYLPYYKRERNKRSTQITVRFLDSPHYSKNIRK
                     KDPILLLHWWKEIFGTILLCIVATTFIVRRLFHPQPHRQRKESETQCQTESKYDSVSA
                     DVSDNSWNDMKYSGYVSRYLTDFEPIQCMGRGGFGVVFEAKNKVDDCNYAIKRIRLPN
                     RELAREKVMREVKALAKLEHPGIVRYFNAWLETPPEKWQEEMDEIWLKDESTDWPLSS
                     PSPMDAPSVKIRRMDPFSTKEQIEVIAPSPERSRSFSVGISCGQTSSSESQFSPLEFS
                     GTDCGDNSDSADAAYNLQDSCLTDCEDVEDGTVDGNDEGHSFELCPSEASPYTRSREG
                     TSSSIVFEDSGCGNASSKEEPRGNRLHDGNHYVNKLTDLKCSSSRSSSEATTLSTSPT
                     RPTTLSLDFTKNTVGQLQPSSPKVYLYIQMQLCRKENLKDWMNRRCSLEDREHGVCLH
                     IFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTP
                     MPAYATHTGQVGTKLYMSPEQIHGNNYSHKVDIFSLGLILFELLYPFSTQMERVRILT
                     DVRNLKFPLLFTQKYPQEHMMVQDMLSPSPTERPEATDIIENAIFENLEFPGKTVLRQ
                     RSRSMSSSGTKHSRQPSCSYSPLPGN"
     exon            508..637
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            638..832
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            833..966
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1201
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1364
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1505
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1628
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1849
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..1962
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2085
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            2086..2235
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..3022
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3190
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..3292
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3355
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..4509
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4487..4492
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /note="hexamer: AATAAA"
     polyA_site      4508
                     /gene="Eif2ak3"
                     /gene_synonym="Pek; Perk"
                     /note="major polyA site"
ORIGIN      
        1 cggaaccctc gctcaatggg cgatgtctgc acaaggctgt cactcaggtg gcagtggctg
       61 agacgtggcc gggcagctct gctgctgcgg cgcgaagtcg agaggcggcg gggtccgtgg
      121 cgcgcgctcg cagtgctccg aggctccgag cggcgagacg ggcgggcgcc gacggcaggg
      181 tctccatgcc cgcgcgtggg gcgggccgct gatggagcgc gccacccggc ccgggccgcg
      241 cgcgctgctg ctgcttctgt tcctgctgct gggctgcgcg gcggggatct cggcggtcgc
      301 gcccgcccgc agtttgcttg ctcccgcgtc ggagacagtg tttggcttag gggcagcggc
      361 cgccccgact tcggccgcgc gggtgcctgc ggtggcaacg gccgaagtga ccgtggagga
      421 cgccgaggca ttgccggctg ccgctggcga accggagtca cgcgcgacgg agcccgatga
      481 cgacgtggaa ctgcggcctc gcggcaggtc cttggtaatc atcagcactt tagatggacg
      541 aatcgctgca ctggatgccg agaatgatgg gaaaaagcag tgggatttgg acgtggggtc
      601 tggttccttg gtttcatcta gcctcagcaa gccagaggtg tttgggaaca agatgatcat
      661 cccctccctg gatggagacc tcttccagtg ggaccgggac cgagagagca tggaggccgt
      721 ccccttcacg gtggagtccc tgctcgaatc ttcctacaag tttggagatg atgttgttct
      781 ggttggaggg aaatctctga ctacatacgg actcagtgct tacagtggaa agctgaggta
      841 tatctgttct gccttgggat gtcgccgatg ggatagtgat gaaatggaag aagaggaaga
      901 catcttgctt ctgcagcgta cgcagaagac tgtgcgagct gtcgggcctc gaagcggcag
      961 tgagaagtgg aatttcagtg ttggccactt tgaacttcgg tatattccag acatggaaac
     1021 tagagccgga ttcattgaaa gcacctttaa accgggtgga aacaaagaag actctaaaat
     1081 tatttcagat gtggaagaac aagaagccac catgctggac acagtgataa aagtttccgt
     1141 tgctgattgg aaggtcatgg cgtttagtag gaagggaggc cgcctggaat gggagtacca
     1201 gttttgtact cccatcgcgt ccgcctggct ggtgagggat ggcaaggtca tccccatcag
     1261 cctgtttgat gatacaagtt acacagccag cgaagaagcc ttgggagacg aagaagacat
     1321 tgtagaggct gctcggggag ccacagagaa cagcgtgtac ttagggatgt acagaggcca
     1381 gctgtacctg cagtcgtccg tcagggtctc agaaaagttc cctacaagcc caaaggcctt
     1441 ggagtctgta aatggcgaaa atgcaattat tcctctgccg acgatcaaat ggaagccctt
     1501 aatccattct ccttctagga ctcctgtctt ggttgggtct gatgaatttg acaaatgtct
     1561 aagtaatgat aagtattccc acgaagaata cagtaatggt gcactttcaa tcctccagta
     1621 tccatacgat aacggttact atctgccata ctacaagaga gaaaggaata agcggagcac
     1681 gcagatcaca gtcaggttcc tggacagccc ccactacagc aagaacatcc gcaagaagga
     1741 ccctatcctc ctgctgcact ggtggaagga gatattcggg acgatcctgc tttgcatcgt
     1801 agccacgacc ttcatcgtgc gcaggctttt ccatcctcag ccccacaggc agcggaagga
     1861 gtctgaaact cagtgccaga ctgaaagtaa atacgactcc gtgagtgccg atgtcagtga
     1921 caacagctgg aatgacatga agtactcagg atacgtatcc cgatatctaa cagattttga
     1981 gccaattcag tgcatgggtc gtggtggctt tggcgttgtc tttgaagcta aaaacaaagt
     2041 agatgactgc aattacgcta tcaagaggat ccggctcccc aacagggagt tggcacggga
     2101 gaaggtaatg cgggaagtta aagccttggc taagctggaa cacccaggca ttgtgaggta
     2161 tttcaacgcc tggctggaaa ccccaccaga gaagtggcaa gaagagatgg atgagatctg
     2221 gctcaaagac gaaagcacag actggccgct cagctcccct agcccgatgg atgccccatc
     2281 tgttaagatc cgaaggatgg atcctttctc tacaaaagag cagatcgaag tcatagctcc
     2341 ttctcctgaa agaagtcggt ctttctcggt gggcatttcc tgtggccaga caagctcatc
     2401 ggagagccag ttctctcccc tggagttctc agggacagac tgcggagaca acagtgactc
     2461 agcggacgca gcctacaacc tccaggacag ttgcctgacg gactgcgagg acgtggaaga
     2521 tggcaccgtg gacggcaatg acgagggaca ctcctttgaa ctttgtccgt ccgaagcttc
     2581 tccctatacc cggtcaaggg aaggaacgtc ctcctccata gtgtttgagg actctggctg
     2641 cggcaacgcg tccagtaagg aggagcccag agggaaccgg ctgcatgatg gcaaccatta
     2701 tgttaataag ctaactgatc tcaagtgctc cagcagcagg tcttcttcag aagccaccac
     2761 cttgtctacc tcccctacca ggccaaccac tctaagcttg gatttcacca agaacactgt
     2821 gggccagctc cagcccagct cccccaaggt gtatctgtac attcagatgc agctgtgcag
     2881 gaaggagaac ctcaaagact ggatgaaccg gcgctgcagc ttggaggacc gggagcacgg
     2941 cgtgtgcctg cacatcttcc tgcagatcgc agaggcagtg gagttcctgc acagcaaggg
     3001 actcatgcac agggacctca agccttccaa catattcttc acaatggatg atgtggtcaa
     3061 ggttggggac tttggactgg tgactgctat ggaccaagat gaagaagagc agactgtact
     3121 gactccaatg ccagcctatg ctacgcacac gggacaagta gggaccaagc tatacatgag
     3181 cccagagcag attcatggaa acaactactc ccataaagtg gacatcttct ctttaggctt
     3241 gattctgttt gaactcctct acccattcag cacccagatg gaacgagtcc ggattttaac
     3301 tgatgtcaga aatctcaagt ttcctctact gttcactcag aaatatcccc aagagcatat
     3361 gatggttcaa gacatgctct ctccatcccc cacggagcgg cctgaagcca cagacatcat
     3421 tgaaaatgcc atatttgaga acttggagtt tcccgggaaa acggttctga gacagcggtc
     3481 ccgctccatg agttcatctg gaacaaaaca ttccagacag cccagctgct cgtacagccc
     3541 actgcctggc aactagccct cagctgccct cgaaggtggc agagcaggca ccctgaggaa
     3601 catggctctc cacagcggtg gactcagatt ttatgctttg atcagttgga ctcgggacca
     3661 atttttctaa gtcagactgg atcacgggcc taacccagtt tgatcttaac tgaacttcaa
     3721 ggaaagggct gtgtaaagga cacatgaact tgttgcttgt cggtgtccca agactagctg
     3781 gtcagcttag aaccttcact tttcaccagg cggtagaaga gatcctcaaa tggtctgaac
     3841 tggaaatgtc tttaaagcac aaaagtgtaa aagaccctct cacatgggaa ctacatgttc
     3901 tagaaacgtg ctttctagag atacaagggt gattttggaa gtggttgtta taaagctgac
     3961 ttcatttttt tccctggtga gccgtgaccc atctgcacta atttgcaagg cacatagcac
     4021 aagctgggtc gccatttatg tcggtagtgt catagtctgc agcagtgaat agcgtcattc
     4081 ttcaggtggt ctagggagcg cgaagagctt ttttgtactt tttacctcca ataatgggaa
     4141 aatgaagctt ttaggtattg gtcaaaagat ctgatttgag agttttgggt ttttttttta
     4201 agtgcagtag gaaatggatt atctattaca actaacttct tcaattatgg aatttttatc
     4261 ctagtagaat tctgtcttaa atgtaatact acaagtgggt acattccccc aaactgatta
     4321 tagataagtt taatcatctc aacttgctaa catgttttca tttttcctgt aaatacgttt
     4381 attttttatt tataaaaatt ctgaaatcaa tccatttggg ttggtggtgt acagaacgca
     4441 cgtaagtgtg ataactatta tgacttcttt caagtctaaa tgatttaata aaaaaatttt
     4501 aaattataca aaaaaaaaa
//
