LOCUS       NM_011163               4343 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus eukaryotic translation initiation factor 2-alpha
            kinase 2 (Eif2ak2), mRNA.
ACCESSION   NM_011163
VERSION     NM_011163.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Yoshida,T., Latt,K.Z., Rosenberg,A.Z., Santo,B.A., Myakala,K.,
            Ishimoto,Y., Zhao,Y., Shrivastav,S., Jones,B.A., Yang,X.,
            Wang,X.X., Tutino,V.M., Sarder,P., Levi,M., Okamoto,K.,
            Winkler,C.A. and Kopp,J.B.
  TITLE     PKR activation-induced mitochondrial dysfunction in HIV-transgenic
            mice with nephropathy
  JOURNAL   Elife 12, 91260 (2024)
   PUBMED   39207915
  REMARK    GeneRIF: PKR activation-induced mitochondrial dysfunction in
            HIV-transgenic mice with nephropathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4343)
  AUTHORS   Zhong,X., Moresco,J.J., SoRelle,J.A., Song,R., Jiang,Y.,
            Nguyen,M.T., Wang,J., Bu,C.H., Moresco,E.M.Y., Beutler,B. and
            Choi,J.H.
  TITLE     Disruption of the ZFP574-THAP12 complex suppresses B cell
            malignancies in mice
  JOURNAL   Proc Natl Acad Sci U S A 121 (31), e2409232121 (2024)
   PUBMED   39047044
REFERENCE   4  (bases 1 to 4343)
  AUTHORS   Luca,D., Lee,S., Hirota,K., Okabe,Y., Uehori,J., Izawa,K.,
            Lanz,A.L., Schutte,V., Sivri,B., Tsukamoto,Y., Hauck,F.,
            Behrendt,R., Roers,A., Fujita,T., Nishikomori,R., Lee-Kirsch,M.A.
            and Kato,H.
  TITLE     Aberrant RNA sensing in regulatory T cells causes systemic
            autoimmunity
  JOURNAL   Sci Adv 10 (9), eadk0820 (2024)
   PUBMED   38427731
REFERENCE   5  (bases 1 to 4343)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 4343)
  AUTHORS   Tanaka,H. and Samuel,C.E.
  TITLE     Sequence of the murine interferon-inducible RNA-dependent protein
            kinase (PKR) deduced from genomic clones
  JOURNAL   Gene 153 (2), 283-284 (1995)
   PUBMED   7533117
REFERENCE   7  (bases 1 to 4343)
  AUTHORS   Ito,T., Jagus,R. and May,W.S.
  TITLE     Interleukin 3 stimulates protein synthesis by regulating
            double-stranded RNA-dependent protein kinase
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7455-7459 (1994)
   PUBMED   7519779
REFERENCE   8  (bases 1 to 4343)
  AUTHORS   Patel,R.C. and Sen,G.C.
  TITLE     Construction and expression of an enzymatically active human-mouse
            chimeric double-stranded RNA-dependent protein kinase
  JOURNAL   J Interferon Res 12 (5), 389-393 (1992)
   PUBMED   1358989
REFERENCE   9  (bases 1 to 4343)
  AUTHORS   Feng,G.S., Chong,K., Kumar,A. and Williams,B.R.
  TITLE     Identification of double-stranded RNA-binding domains in the
            interferon-induced double-stranded RNA-activated p68 kinase
  JOURNAL   Proc Natl Acad Sci U S A 89 (12), 5447-5451 (1992)
   PUBMED   1351683
REFERENCE   10 (bases 1 to 4343)
  AUTHORS   Icely,P.L., Gros,P., Bergeron,J.J., Devault,A., Afar,D.E. and
            Bell,J.C.
  TITLE     TIK, a novel serine/threonine kinase, is recognized by antibodies
            directed against phosphotyrosine
  JOURNAL   J Biol Chem 266 (24), 16073-16077 (1991)
   PUBMED   1714905
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK028602.1 and BU756642.1.
            
            On Apr 18, 2009 this sequence version replaced NM_011163.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028602.1, BC016422.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2394              AK028602.1         1-2394
            2395-4312           AK028602.1         2396-4313
            4313-4343           BU756642.1         1-31                c
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 49.56 cM"
     gene            1..4343
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /note="eukaryotic translation initiation factor 2-alpha
                     kinase 2"
                     /db_xref="GeneID:19106"
                     /db_xref="MGI:MGI:1353449"
     exon            1..173
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            174..305
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..1737
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="protein kinase, interferon-inducible double
                     stranded RNA dependent; p68 kinase; eIF-2A protein kinase
                     2; P1/eIF-2A protein kinase; protein kinase RNA-activated;
                     serine/threonine-protein kinase TIK; interferon-inducible
                     RNA-dependent protein kinase; dsRNA-activated kinase;
                     T-cell viral integration site; eIF-2 alpha; IFN- type
                     I-induced and dsRNA-activated kinase; IFN-induced and
                     double-stranded RNA-activated kinase; tyrosine-protein
                     kinase EIF2AK2"
                     /codon_start=1
                     /product="interferon-induced, double-stranded
                     RNA-activated protein kinase"
                     /protein_id="NP_035293.1"
                     /db_xref="CCDS:CCDS28980.1"
                     /db_xref="GeneID:19106"
                     /db_xref="MGI:MGI:1353449"
                     /translation="MASDTPGFYMDKLNKYRQMHGVAITYKELSTSGPPHDRRFTFQV
                     LIDEKEFPEAKGRSKQEARNAAAKLAVDILDNENKVDCHTSASEQGLFVGNYIGLVNS
                     FAQKKKLSVNYEQCEPNSELPQRFICKCKIGQTMYGTGSGVTKQEAKQLAAKEAYQKL
                     LKSPPKTAGTSSSVVTSTFSGFSSSSSMTSNGVSQSAPGSFSSENVFTNGLGENKRKS
                     GVKVSPDDVQRNKYTLDARFNSDFEDIEEIGLGGFGQVFKAKHRIDGKRYAIKRVKYN
                     TEKAEHEVQALAELNHVNIVQYHSCWEGVDYDPEHSMSDTSRYKTRCLFIQMEFCDKG
                     TLEQWMRNRNQSKVDKALILDLYEQIVTGVEYIHSKGLIHRDLKPGNIFLVDERHIKI
                     GDFGLATALENDGKSRTRRTGTLQYMSPEQLFLKHYGKEVDIFALGLILAELLHTCFT
                     ESEKIKFFESLRKGDFSNDIFDNKEKSLLKKLLSEKPKDRPETSEILKTLAEWRNISE
                     KKKRNTC"
     exon            306..426
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            427..563
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            564..684
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            685..764
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            765..813
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            814..848
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            849..899
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1022
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1142
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1323
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1455
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1557
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1614
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..4326
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2344..2349
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /note="hexamer: ATTAAA"
     polyA_site      2371
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /note="major polyA site"
     regulatory      4303..4308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /note="hexamer: AATAAA"
     polyA_site      4320
                     /gene="Eif2ak2"
                     /gene_synonym="2310047A08Rik; 4732414G15Rik; Pkr; Prkr;
                     Tik"
                     /note="major polyA site"
ORIGIN      
        1 gaaggcggag tccgccggga aaacgaaaca gaagagaacc ggccaggccc ggacttccat
       61 gggcagcagc agcggcaggg aacggagggc gaatagattt cagagcctgc acctgaagta
      121 caattcgaat cctgctccag ggagcgagcc actgtccgga tccagaaact ttggccactg
      181 ggaggaaaaa tggccagtga taccccaggt ttctacatgg acaaacttaa taaataccgc
      241 cagatgcacg gagtagccat tacgtataaa gaacttagta cttcgggacc tccacatgac
      301 agaaggttta catttcaagt tttaatagat gagaaggaat ttccagaagc caaaggtaga
      361 tcaaagcagg aggcaagaaa cgctgcagcc aaattagctg ttgatatact tgataacgaa
      421 aacaaggtgg attgtcacac gagtgcatct gagcaaggct tgttcgttgg taactacata
      481 ggccttgtca atagctttgc ccagaagaaa aagctgtctg taaattatga acagtgtgag
      541 cccaactctg agttgcctca aagatttatt tgtaaatgca aaattgggca gacaatgtat
      601 ggtactggtt caggtgtcac caaacaggag gcaaagcagt tggctgcgaa agaagcctat
      661 cagaagctgt taaagagccc gccgaaaact gccggaacat cctctagcgt tgtcacatct
      721 acattcagtg gcttttccag cagctcgtct atgacaagta atggtgtttc ccagtcagca
      781 cctggaagtt tttcctcaga gaacgtgttt acgaacggtc tcggagaaaa taaaaggaaa
      841 tcaggagtaa aagtatcccc tgatgatgtg caaagaaata aatatacctt ggacgccagg
      901 tttaacagcg attttgaaga catagaagaa attggcttag gtggatttgg tcaagttttc
      961 aaagcgaaac acagaattga tggaaagaga tacgctatta agcgcgttaa atataacacg
     1021 gagaaggcgg agcacgaagt acaagcgctg gcagaactca atcacgtcaa cattgtccaa
     1081 taccatagtt gttgggaggg agttgactat gatcctgagc acagcatgag tgatacaagt
     1141 cgatacaaaa cccggtgcct ctttattcaa atggaattct gtgataaagg aactttggag
     1201 caatggatga gaaacagaaa tcagagtaaa gtggacaaag ctttgatttt ggacttatat
     1261 gaacaaatcg tgaccggagt ggagtatata cactcgaaag ggttaattca cagagatctt
     1321 aagccaggta atatattttt agtagatgaa agacacatta agatcggaga ctttggcctt
     1381 gcaacagccc tggaaaatga tggaaaatcc cgaacaagga gaacaggaac tcttcaatac
     1441 atgagtccag aacagttatt tttaaagcac tatggaaaag aagtggacat ctttgctttg
     1501 ggccttattc tagctgaact tcttcacacg tgcttcacgg agtcagagaa aataaagttt
     1561 ttcgaaagtc taagaaaagg cgacttctct aatgatatat tcgacaacaa agaaaaaagc
     1621 cttctaaaaa aactactctc agagaaaccc aaggaccgac ctgagacatc tgaaatcctg
     1681 aagaccttgg ctgaatggag gaacatctca gagaaaaaga aaagaaacac atgttagggc
     1741 ctttctgaga aaacattcct ctgccgtggt tttcctttaa cgatctgcag tctgagggga
     1801 gtatcagtga atattatcct tcttttctta ataccactct cccagacagg ttttggttag
     1861 ggtgacccac agacattgta tttattaggc tatgaaaaag tatgcccatt tcctcaattg
     1921 ttaattgctg ggcctgtggc tggctagcta gccaaatatg taaatgcttg tttctcgtct
     1981 gcccaaagag aaaggcaggc tcctgtgtgg gaagtcacag agcccccaaa gccaactgga
     2041 tgaggaagga ctctggcttt tggcataaaa aagagctggt agtcagagct ggggcagaag
     2101 gtcctgcaga cagacagaca gacagacaga cagacagaca gacagacaga gacacaaaga
     2161 catggactag aatggaggag ggagggagga agggagggag ggagagagag agagagaaag
     2221 aaagagagag agaccacatg gagagacaaa atggcttaag ttagctgggc tacctgagag
     2281 actgtcccag aaaacaggcc aacaaccttc cttatgctat atagatgtct cagtgtcttt
     2341 atcattaaac accaagcagg actgctaaaa actctgcaat agggtttttt ttttcctgtt
     2401 acttcaaaag caatcttaca aagttatttt tttgacaatt ccatacatgc attgtgttct
     2461 gatcccactc tgaaccctct gccattcatg ccttgtctgt catgtgaact gttgcctctg
     2521 aatgtggggg tccaaattaa ccctctgccc ttgagtggct tctctcaggt agtgattgtg
     2581 atgagaaaag taatgagatg ctggcaaaga tgtgcagaaa gaagaacact tctccactgc
     2641 tggtaggatt gcaagctggt acaaccaccc tggaaatcag actggaggtt cctcagaaac
     2701 acagtactac ctgaggaccc aacaatacca ctactggtca tatacccaga agatggtcca
     2761 acatgtaata tggacacatg cgccactatg ttcatagtag ccttatttat aatagccagg
     2821 agctggaaag aacccagatg tccctcagca gaggaatgga tacagaaaat gtggcacatt
     2881 tacacaatgg agtactactc agctattaaa aatgaattca tgaaattctt agacaaatgg
     2941 atggatctgg aggatatcat cttgagtgag gtaacccaat cgcaaaagaa cacacatgat
     3001 atgcactcac tgataagtgg atattagccc aaaagctcca aataaccaag atacaattca
     3061 cagactacat gaagctcaag aagaaggaag accaaagtgt gggtgctttg gtccctctta
     3121 gaaggggaac aaagtactca caggagcaaa tatggagata gagtgtagag cagagactga
     3181 aggaaaggcc atccagagac tgtcccatat acagagactg ggaattcatc ccatacacag
     3241 ttaccaaacc cagacactat tgtggatgcc aagacattca tgctgacagg agcctgatat
     3301 ggctgtctcc tgagaggtcc tgccagagcc ttacaataca gagactgatg ctcacagcca
     3361 accactggac tgagtgtggg gtccccaata gaggagttag agaaaggact gaaggagttg
     3421 aaggggtttg caaccccata agaacaacaa tatcaaccaa gcagaccccc cagagctccc
     3481 agtgactaag ccatcaacca aggagtacac atggctccag ctgcatatgt agcagaggat
     3541 ggccttgtca tgtatcaaaa ggaggagagg tccttggtcc tatgaaggtg cgatagatgc
     3601 cccagtatag gggaatcaag ggcagatagg tgggttggag gaacaccctc atagaagcag
     3661 ggggagtaag gaaggatatg gggatttctg ggaggggtgg aaactaggaa agggggtaac
     3721 atttgaaatg taaataaaga aaatatccaa ttaaaaaaaa aagaaaaaga aaaaagaaaa
     3781 gaatagtaat aaaatggtac aggaagtaga gttatattgc aataaaccta ctgttgggct
     3841 ttcaggactg gtttgtggga ggaatgtgaa aaagtttgaa gccccaggtt agagaagtcc
     3901 tcaaatggta tacgtcaaac ttactgtggt agctcaaaag tctcctgaga ggccctgctt
     3961 ggagttagcc ttgtagaggt ccagtctttc cttgttgttc tttcagactt gctttgtaga
     4021 atattggtag ttactttgtg cctttgtatg ctgtaatagt tgttttatag ggcctcacag
     4081 ctaagagttt ctcgctgctt ctcaaagcac tttggacctt tgcatggagt tgagtattaa
     4141 gattatggga atttctgagg tgggactgaa agcattttgc attatgagat ggccatgagc
     4201 caacagagac ttggacacac tcctccactg tcaaccgagg cttctgccaa atcttccctg
     4261 tcatgaagga ttgtatcatc tgaaattgag tctaaataga taaataaata agtaaataaa
     4321 tctctcaaaa aaaaaaaaaa aaa
//
