LOCUS       NM_001005509            2077 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus eukaryotic translation initiation factor 2A (Eif2a),
            transcript variant 1, mRNA.
ACCESSION   NM_001005509
VERSION     NM_001005509.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Xu,L., Tan,C., Barr,J., Talaba,N., Verheyden,J., Chin,J.S.,
            Gaboyan,S., Kasaraneni,N., Elgamal,R.M., Gaulton,K.J., Lin,G.,
            Afshar,K., Golts,E., Meier,A., Crotty Alexander,L.E., Borok,Z.,
            Shen,Y., Chung,W.K., McCulley,D.J. and Sun,X.
  TITLE     Context-dependent roles of mitochondrial LONP1 in orchestrating the
            balance between airway progenitor versus progeny cells
  JOURNAL   Cell Stem Cell 31 (10), 1465-1483 (2024)
   PUBMED   39181129
REFERENCE   3  (bases 1 to 2077)
  AUTHORS   Ding,S.M., Shi,L.G., Xing,F., Cui,S.S., Cheng,H.R., Liu,Y.,
            Ji,D.M., Liang,D., Cao,Y.X. and Liu,Y.J.
  TITLE     Melatonin Protects Against Mitochondrial Dyshomeostasis and Ovarian
            Damage Caused by Chronic Unpredictable Mild Stress Through the
            eIF2alpha-AFT4 Signaling Pathway in Mice
  JOURNAL   Reprod Sci 31 (10), 3191-3201 (2024)
   PUBMED   39060751
  REMARK    GeneRIF: Melatonin Protects Against Mitochondrial Dyshomeostasis
            and Ovarian Damage Caused by Chronic Unpredictable Mild Stress
            Through the eIF2alpha-AFT4 Signaling Pathway in Mice.
REFERENCE   4  (bases 1 to 2077)
  AUTHORS   Li,Q., Jiang,S., Lei,K., Han,H., Chen,Y., Lin,W., Xiong,Q., Qi,X.,
            Gan,X., Sheng,R., Wang,Y., Zhang,Y., Ma,J., Li,T., Lin,S., Zhou,C.,
            Chen,D. and Yuan,Q.
  TITLE     Metabolic rewiring during bone development underlies tRNA
            m7G-associated primordial dwarfism
  JOURNAL   J Clin Invest 134 (20), e177220 (2024)
   PUBMED   39255038
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2077)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2077)
  AUTHORS   Scheuner,D., Patel,R., Wang,F., Lee,K., Kumar,K., Wu,J.,
            Nilsson,A., Karin,M. and Kaufman,R.J.
  TITLE     Double-stranded RNA-dependent protein kinase phosphorylation of the
            alpha-subunit of eukaryotic translation initiation factor 2
            mediates apoptosis
  JOURNAL   J Biol Chem 281 (30), 21458-21468 (2006)
   PUBMED   16717090
  REMARK    GeneRIF: Double-stranded RNA-dependent protein kinase
            phosphorylation of the alpha-subunit of eukaryotic translation
            initiation factor 2 mediates apoptosis
REFERENCE   7  (bases 1 to 2077)
  AUTHORS   Hori,O., Miyazaki,M., Tamatani,T., Ozawa,K., Takano,K., Okabe,M.,
            Ikawa,M., Hartmann,E., Mai,P., Stern,D.M., Kitao,Y. and Ogawa,S.
  TITLE     Deletion of SERP1/RAMP4, a component of the endoplasmic reticulum
            (ER) translocation sites, leads to ER stress
  JOURNAL   Mol Cell Biol 26 (11), 4257-4267 (2006)
   PUBMED   16705175
REFERENCE   8  (bases 1 to 2077)
  AUTHORS   Datta,B., Majumdar,A., Datta,R. and Balusu,R.
  TITLE     Treatment of cells with the angiogenic inhibitor fumagillin results
            in increased stability of eukaryotic initiation factor 2-associated
            glycoprotein, p67, and reduced phosphorylation of extracellular
            signal-regulated kinases
  JOURNAL   Biochemistry 43 (46), 14821-14831 (2004)
   PUBMED   15544353
REFERENCE   9  (bases 1 to 2077)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2077)
  AUTHORS   Ito,T., Jagus,R. and May,W.S.
  TITLE     Interleukin 3 stimulates protein synthesis by regulating
            double-stranded RNA-dependent protein kinase
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7455-7459 (1994)
   PUBMED   7519779
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121794.3 and AC119873.38.
            
            On Jan 14, 2022 this sequence version replaced NM_001005509.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK078519.1, BC082557.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC121794.3         19694-19737         c
            45-114              AC121794.3         14624-14693         c
            115-189             AC121794.3         8172-8246           c
            190-308             AC121794.3         6134-6252           c
            309-408             AC121794.3         4641-4740           c
            409-491             AC121794.3         4064-4146           c
            492-565             AC121794.3         712-785             c
            566-710             AC121794.3         408-552             c
            711-827             AC121794.3         164-280             c
            828-1399            AC119873.38        25871-26442
            1400-1513           AC119873.38        30047-30160
            1514-1630           AC119873.38        33013-33129
            1631-1696           AC119873.38        34051-34116
            1697-2077           AC119873.38        34599-34979
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 28.58 cM"
     gene            1..2077
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /note="eukaryotic translation initiation factor 2A"
                     /db_xref="GeneID:229317"
                     /db_xref="MGI:MGI:1098684"
     exon            1..44
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1762
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /note="isoform 1 is encoded by transcript variant 1;
                     eIF-2A"
                     /codon_start=1
                     /product="eukaryotic translation initiation factor 2A
                     isoform 1"
                     /protein_id="NP_001005509.1"
                     /db_xref="CCDS:CCDS17367.1"
                     /db_xref="GeneID:229317"
                     /db_xref="MGI:MGI:1098684"
                     /translation="MAPSTPLLTVRGSEGLYMVNGPPHFTESTVLPRESGRNCKVYTF
                     SKDGTLFAWSNGEKVNIINVANKGLLHSFDLPKAVCLEFSPNNTVLATWQPYTTSKDG
                     TAGTPNLQLYDMKTGACLKSFIQKKMQNWCPSWSDDEIICARNVNNEVHFFENNNFNT
                     IANKLHLQKVNDFNLSPGTQPYKVAVYVPGSKGAPSFVRLYQYPNFAGPQAALANKSF
                     FKADKVTMLWNKKATAVLVIASTEVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPI
                     YDVVWNSSSTEFCAVYGFMPAKATVFNLKCDPVFDFGTGPRNAAFYSPHGHILVLAGF
                     GNLRGQMEVWDVKNYKLISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHY
                     TGSLLHKYDVPSNGELWQVSWQPFLDGIFPAKTIKYQAVPSEVPSEEPKVATAYRPPA
                     LRNKPVTNSKLHEEEPPQNMKPHPGSDKPLSKTALKNQRKHEAKKAAKQEARSDAAPT
                     PVPQSAPRNTVTQSASGDPEVDKKIKNLKKKLKAIEQLKEQAAAGKQLEKNQLEKIQK
                     ETALLQELEDLELGV"
     exon            45..114
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            115..189
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            190..308
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            309..408
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            409..491
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            492..565
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            566..710
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            711..827
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            828..1399
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1513
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1630
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1696
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..2077
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1964..1969
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /note="hexamer: AATAAA"
     polyA_site      1998
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
     regulatory      2057..2062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /note="hexamer: AATAAA"
     polyA_site      2077
                     /gene="Eif2a"
                     /gene_synonym="D030048D22; D3Ertd194e"
                     /note="major polyA site"
ORIGIN      
        1 ctctttccgg gacaagatgg cgccgtccac accgctgttg acagtcagag gatcagaggg
       61 actgtacatg gtgaatggac caccacactt cacagaaagc acggtgcttc ccagagaatc
      121 tggaagaaat tgcaaagtgt acacttttag taaggatggg acattgtttg cctggagcaa
      181 tggagaaaaa gtaaatataa tcaatgtcgc taacaaggga ctactgcact ccttcgacct
      241 cccaaaagca gtttgccttg aattctcacc aaataacact gtcctggcaa cgtggcagcc
      301 ttacactact tctaaagatg gcactgctgg gacgcctaac ctacaacttt atgacatgaa
      361 aactggagca tgtttgaaat cgttcatcca gaaaaaaatg caaaattggt gtccttcctg
      421 gtcagatgat gaaattattt gtgcccggaa tgttaacaac gaagttcact tctttgagaa
      481 caacaatttt aacacaattg caaataaatt acatttgcaa aaagttaatg actttaattt
      541 atcacctgga acccaacctt ataaggtggc agtttatgtc ccaggaagta aaggtgcccc
      601 ctcatttgtt cgactgtatc agtatcccaa ctttgccgga cctcaggcag cactggccaa
      661 taaaagcttc tttaaagctg ataaggttac aatgctgtgg aataaaaaag ctactgctgt
      721 gctggtaata gccagtacag aggttgacaa gacaggagcc tcctactatg gagaacaaac
      781 actgcactac attgcaacaa atggagaaag tgctgtggta caattaccaa aaaatggccc
      841 aatttatgat gtagtttgga attccagttc tactgagttt tgcgctgttt atggttttat
      901 gcctgcgaaa gcaacagttt tcaacctgaa gtgtgatcct gtgtttgact ttgggactgg
      961 tcctcgcaac gcagccttct atagccctca tggacatata ttagtgctgg ctggatttgg
     1021 aaatcttcga ggacaaatgg aagtatggga tgttaaaaac tacaagctta tttctaagcc
     1081 agtggcttct gattctacat attttgcctg gtgcccagat ggtgagcata tcttaacagc
     1141 cacatgtgct cctaggttac gtgttaataa tgggtataag atctggcatt atactggctc
     1201 tcttttgcat aagtatgatg tgccatcaaa tggagaatta tggcaggttt cttggcagcc
     1261 atttttggat ggaatatttc cagcaaaaac gataaagtac caagcagttc caagtgaagt
     1321 gcctagtgag gagcctaaag ttgcaacagc ttatagacct ccagccttaa gaaataagcc
     1381 agtcaccaat tccaaactgc atgaagagga acctccccag aatatgaagc cacatccagg
     1441 aagtgacaag ccattatcaa aaacagccct taaaaatcaa aggaagcatg aagctaaaaa
     1501 agctgcaaaa caggaagcaa gaagtgatgc ggctcctact cctgtcccac agagtgcacc
     1561 acggaacacg gtcacccagt ctgcttctgg tgatcctgaa gtagacaaaa aaattaagaa
     1621 tctaaagaag aaactgaaag ccatagagca gctaaaagag caggcagccg ccgggaaaca
     1681 gctagaaaaa aatcagttgg agaaaattca gaaagagaca gcccttctcc aagagcttga
     1741 agatttggaa ttgggagttt gaagactcac agaaagtgaa tttgttggca ggaaacacta
     1801 taagcaagtc tcttaagccc atggtgttca tggaattgcc atatgcccac tgttaacatc
     1861 agtctatatt tttaaccatt tatccaaggt tgaatatgta taattatcta atgatgtcta
     1921 ttcaattgac tttacatctg cattctgtat catgtcagta tgtaataaag agagatgatt
     1981 ttttccagat aagcttaata aattgacaga catttggttt ttttttaaat gggtaatagt
     2041 atttcctatg taaacaaata aagacatttt aaatttg
//
