LOCUS       NM_001199494            2167 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus etoposide induced 2.4 mRNA (Ei24), transcript variant
            1, mRNA.
ACCESSION   NM_001199494
VERSION     NM_001199494.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Zhang,X., Mao,Y., Peng,W., Liu,H., Liang,L., Wang,D., Liu,L.,
            Zhou,Y., Zhang,F., Xiao,Y., Shi,M., Shao,S., Wang,Y., Guo,B. and
            Zhang,X.
  TITLE     Autophagy-related protein EI24 delays the development of pulmonary
            fibrosis by promoting autophagy
  JOURNAL   Life Sci 264, 118664 (2021)
   PUBMED   33127511
  REMARK    GeneRIF: Autophagy-related protein EI24 delays the development of
            pulmonary fibrosis by promoting autophagy.
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Mao,Y., Zhang,X., Peng,W., Liu,H., Zhou,X., Liang,L., Xiang,J.,
            Zhang,H., Wang,D., Liu,L., Zhou,Y., Zhang,F., Xiao,Y., Shi,M.,
            Wang,Y. and Guo,B.
  TITLE     EI24 alleviates renal interstitial fibrosis through inhibition of
            epithelial-mesenchymal transition and fibroblast activation
  JOURNAL   FASEB J 35 (1), e21239 (2021)
   PUBMED   33368642
  REMARK    GeneRIF: EI24 alleviates renal interstitial fibrosis through
            inhibition of epithelial-mesenchymal transition and fibroblast
            activation.
REFERENCE   3  (bases 1 to 2167)
  AUTHORS   Yuan,L., Liu,Q., Wang,Z., Hou,J. and Xu,P.
  TITLE     EI24 tethers endoplasmic reticulum and mitochondria to regulate
            autophagy flux
  JOURNAL   Cell Mol Life Sci 77 (8), 1591-1606 (2020)
   PUBMED   31332481
  REMARK    GeneRIF: This study shows that the autophagy process is inhibited
            in EI24-deficient primary pancreatic beta cells.
            Erratum:[Cell Mol Life Sci. 2020 Jun;77(11):2255-2256. doi:
            10.1007/s00018-019-03355-3. PMID: 31754725]
REFERENCE   4  (bases 1 to 2167)
  AUTHORS   Nam,T.W., Park,S.Y., Lee,J.H., Roh,J.I. and Lee,H.W.
  TITLE     Effect of EI24 expression on the tumorigenesis of ApcMin/+
            colorectal cancer mouse model
  JOURNAL   Biochem Biophys Res Commun 514 (4), 1087-1092 (2019)
   PUBMED   31097220
  REMARK    GeneRIF: The Ei24 plays no significant role in colon cancer caused
            by the substitutional mutation of Apc in mice.
REFERENCE   5  (bases 1 to 2167)
  AUTHORS   Yuan,L., Wang,H., Liu,Q., Wang,Z., Zhang,M., Zhao,Y., Liang,K.,
            Chen,L., Xu,T. and Xu,P.
  TITLE     Etoposide-induced protein 2.4 functions as a regulator of the
            calcium ATPase and protects pancreatic beta-cell survival
  JOURNAL   J Biol Chem 293 (26), 10128-10140 (2018)
   PUBMED   29769309
  REMARK    GeneRIF: Etoposide-induced protein 2.4 functions as a regulator of
            the calcium ATPase and protects pancreatic beta-cell survival
REFERENCE   6  (bases 1 to 2167)
  AUTHORS   McClive,P.J., Hurley,T.M., Sarraj,M.A., van den Bergen,J.A. and
            Sinclair,A.H.
  TITLE     Subtractive hybridisation screen identifies sexually dimorphic gene
            expression in the embryonic mouse gonad
  JOURNAL   Genesis 37 (2), 84-90 (2003)
   PUBMED   14595844
REFERENCE   7  (bases 1 to 2167)
  AUTHORS   Kannan,K., Kaminski,N., Rechavi,G., Jakob-Hirsch,J., Amariglio,N.
            and Givol,D.
  TITLE     DNA microarray analysis of genes involved in p53 mediated
            apoptosis: activation of Apaf-1
  JOURNAL   Oncogene 20 (26), 3449-3455 (2001)
   PUBMED   11423996
REFERENCE   8  (bases 1 to 2167)
  AUTHORS   Gu,Z., Flemington,C., Chittenden,T. and Zambetti,G.P.
  TITLE     ei24, a p53 response gene involved in growth suppression and
            apoptosis
  JOURNAL   Mol Cell Biol 20 (1), 233-241 (2000)
   PUBMED   10594026
REFERENCE   9  (bases 1 to 2167)
  AUTHORS   Gu,Z., Gilbert,D.J., Valentine,V.A., Jenkins,N.A., Copeland,N.G.
            and Zambetti,G.P.
  TITLE     The p53-inducible gene EI24/PIG8 localizes to human chromosome
            11q23 and the proximal region of mouse chromosome 9
  JOURNAL   Cytogenet Cell Genet 89 (3-4), 230-233 (2000)
   PUBMED   10965130
REFERENCE   10 (bases 1 to 2167)
  AUTHORS   Lehar,S.M., Nacht,M., Jacks,T., Vater,C.A., Chittenden,T. and
            Guild,B.C.
  TITLE     Identification and cloning of EI24, a gene induced by p53 in
            etoposide-treated cells
  JOURNAL   Oncogene 12 (6), 1181-1187 (1996)
   PUBMED   8649819
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155921.2.
            
            [WARNING] On Aug 7, 2024 this sequence was replaced by
            NM_001199494.3.
            
            On Apr 10, 2019 this sequence version replaced NM_001199494.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK172233.1, AK159927.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AC155921.2         22798-22919
            123-232             AC155921.2         26783-26892
            233-378             AC155921.2         30207-30352
            379-439             AC155921.2         31879-31939
            440-506             AC155921.2         33583-33649
            507-631             AC155921.2         34072-34196
            632-751             AC155921.2         34480-34599
            752-863             AC155921.2         35553-35664
            864-975             AC155921.2         37288-37399
            976-1050            AC155921.2         37717-37791
            1051-2167           AC155921.2         39863-40979
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 20.68 cM"
     gene            1..2167
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /note="etoposide induced 2.4 mRNA"
                     /db_xref="GeneID:13663"
                     /db_xref="MGI:MGI:108090"
     exon            1..122
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            123..232
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..1213
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /note="p53-induced gene 8 protein"
                     /codon_start=1
                     /product="etoposide-induced protein 2.4"
                     /protein_id="NP_001186423.2"
                     /db_xref="CCDS:CCDS22973.2"
                     /db_xref="GeneID:13663"
                     /db_xref="MGI:MGI:108090"
                     /translation="MADSVKTFLQDLGRGIKDSIWGICTISKLDARIQQKREEQRRRR
                     ASSLLAQRRPQSVERKQESEPRIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSVTA
                     RIIGDPSLHGDVWSWLEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAFEVSGRK
                     PHPFPSVSKIIADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLYCFE
                     YRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFIIS
                     ANEAKTPGKAYLFQLRLFSLVVFLSNRLFHKTVYLQSALSSSSSAEKFPSPHPSPAKL
                     KAAAGH"
     exon            233..378
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            379..439
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            440..506
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            507..631
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            632..751
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            752..863
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            864..975
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1050
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..2167
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2140..2145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /note="hexamer: ATTAAA"
     polyA_site      2165
                     /gene="Ei24"
                     /gene_synonym="PIG8"
                     /note="major polyA site"
ORIGIN      
        1 agtgtgggcg cagccccggc tctccgagcg cccgggcgga gcgggcagcc gggcgcggag
       61 caggcgaacc gagcctcggt gggcgagcgc cctggctgga gccgcggcgg tagggtggcg
      121 gggccctcca tgatgaatgg tttgggggca cttccctctc gctgtatttg atagtctggg
      181 cagtggagag atggctgaca gtgtcaaaac ctttctgcag gaccttggca ggggaatcaa
      241 agactccatc tggggcatct gtaccatctc aaagctagat gctcggatcc agcagaagag
      301 agaggaacag cgtcgaagaa gggcaagtag cctcttggcc cagaggagac cccagagtgt
      361 agagcggaag caagagagtg aaccacgtat tgttagtaga attttccagt gttgtgcttg
      421 gaatggtgga gtattctggt tcagtctcct cttgttttat cgagtgttta ttcctgtact
      481 tcagtcagta acagcccgga ttattggaga tccatcactt catggagatg tttggtcatg
      541 gctggaattc ttcctcacat caattttcag tgctctttgg gtgctccccc tgtttgtgct
      601 tagcaaagtt gtgaatgcca tttggttcca agatatagct gacttggcat ttgaagtatc
      661 agggaggaaa cctcatccat tccccagtgt cagcaaaata attgctgaca tgctcttcaa
      721 ccttttgcta caggcacttt tccttattca ggggatgttt gtgagtctct tccccatcca
      781 tcttgtgggt cagctggtta gtctgctgca tatgtctctt ctctattcac tgtactgctt
      841 tgagtaccgt tggttcaaca aaggaattga aatgcaccag cgattgtcga acatagaaag
      901 gaattggcct tactactttg ggtttggctt gcccttggct ttcctcacag caatgcaatc
      961 ctcctacatt atcagtggct gcctcttttc tatcctgttt cctttattca tcatcagcgc
     1021 caatgaagca aagactcctg gaaaagcata tcttttccag ttgcgcctat tctccttggt
     1081 ggtcttttta agcaacagac ttttccacaa gaccgtctac ctgcagtcag ccctgagcag
     1141 ctcgtcctct gcagagaaat tcccttcgcc acatccttct cctgccaaac tgaaagctgc
     1201 tgcaggccac tgagccctgc tgtcaaaggg gtgggtggga ctgggtggag gatgtggcag
     1261 ctcttttctc tgttttcctc cccctgccgt ggaaggcaga acccactgcc aagggccctc
     1321 tgcatagtcc cttgtctttg aattggaatc ttcctgactc cagtatatgg atttttacca
     1381 ccaccctagg tctgtaagga ccagagtttt ccagctgttt ttttagcact tgccagctcc
     1441 tgtgcctgga ctgattgatt tgagtacttt tttccccctt tccttgtgtc atttaccctc
     1501 ccacttcctc ctgccttcca gcacccctgg atgaatgggc tttgtaattt taactgttgt
     1561 attttgtgaa tttgttgtta ctgtttttct gtgaagcaca tacattgtat gtgggaggta
     1621 aaggggcatt ccagttgctc cagtcactcc ctctatagcc atcactgtct tgttttctgt
     1681 aactcaggtt aggttttggt ctctattgct ctgctgcaga aaaggaaaga aaggagtggg
     1741 ggaaatggag cctgaagagt tggggcagat agacctcagc caaactggct gggttttgag
     1801 gagtcatgtt ctttcttccc cttgaagggg aaagagtttt ttccactggt ccatttaaag
     1861 tttcccagct atggggtggt accagttctg gacaagtgcc actgcatcat agtatgctcg
     1921 gagaatctga accttactct gaagatgaaa tttactgttg gccactgcca ggtcagactg
     1981 gtgttttaag gaatactggg tgcttcatat aggaactgaa ggggtaaact tactaaacca
     2041 ttcaacctgt gattggtgat gttttcctgt cattttaaga gtcgacacat gggtgggggg
     2101 gcagatgtaa aaaaacttgt acaattttaa aatatcacaa ttaaacgtga gctggtttcc
     2161 caaaata
//
