LOCUS       NM_133838               3359 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus EH-domain containing 4 (Ehd4), transcript variant 1,
            mRNA.
ACCESSION   NM_133838
VERSION     NM_133838.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Malinova,T.S., Angulo-Urarte,A., Nuchel,J., Tauber,M., van der
            Stoel,M.M., Janssen,V., de Haan,A., Groenen,A.G., Tebbens,M.,
            Graupera,M., Plomann,M. and Huveneers,S.
  TITLE     A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin
            trafficking for endothelial migration and angiogenesis
  JOURNAL   Nat Commun 12 (1), 2610 (2021)
   PUBMED   33972531
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Iseka,F.M., Goetz,B.T., Mushtaq,I., An,W., Cypher,L.R.,
            Bielecki,T.A., Tom,E.C., Arya,P., Bhattacharyya,S., Storck,M.D.,
            Semerad,C.L., Talmadge,J.E., Mosley,R.L., Band,V. and Band,H.
  TITLE     Role of the EHD Family of Endocytic Recycling Regulators for TCR
            Recycling and T Cell Function
  JOURNAL   J Immunol 200 (2), 483-499 (2018)
   PUBMED   29212907
REFERENCE   3  (bases 1 to 3359)
  AUTHORS   Rahman,S.S., Moffitt,A.E.J., Trease,A.J., Foster,K.W., Storck,M.D.,
            Band,H. and Boesen,E.I.
  TITLE     EHD4 is a novel regulator of urinary water homeostasis
  JOURNAL   FASEB J 31 (12), 5217-5233 (2017)
   PUBMED   28778975
REFERENCE   4  (bases 1 to 3359)
  AUTHORS   Yeow,I., Howard,G., Chadwick,J., Mendoza-Topaz,C., Hansen,C.G.,
            Nichols,B.J. and Shvets,E.
  TITLE     EHD Proteins Cooperate to Generate Caveolar Clusters and to
            Maintain Caveolae during Repeated Mechanical Stress
  JOURNAL   Curr Biol 27 (19), 2951-2962 (2017)
   PUBMED   28943089
  REMARK    GeneRIF: EHD1, EHD2, and EHD4 are recruited to caveolae.
            Recruitment of the other EHDs increases markedly when EHD2, which
            has been previously detected at caveolae, is absent. Construction
            of knockout cell lines lacking EHDs 1, 2, and 4 confirms this
            apparent functional redundancy.
REFERENCE   5  (bases 1 to 3359)
  AUTHORS   Bhattacharyya,S., Rainey,M.A., Arya,P., Mohapatra,B.C., Mushtaq,I.,
            Dutta,S., George,M., Storck,M.D., McComb,R.D., Muirhead,D.,
            Todd,G.L., Gould,K., Datta,K., Gelineau-van Waes,J., Band,V. and
            Band,H.
  TITLE     Endocytic recycling protein EHD1 regulates primary cilia
            morphogenesis and SHH signaling during neural tube development
  JOURNAL   Sci Rep 6, 20727 (2016)
   PUBMED   26884322
  REMARK    Erratum:[Sci Rep. 2017 Mar 23;7:42320. doi: 10.1038/srep42320..
            PMID: 28332491]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3359)
  AUTHORS   George,M., Rainey,M.A., Naramura,M., Ying,G., Harms,D.W.,
            Vitaterna,M.H., Doglio,L., Crawford,S.E., Hess,R.A., Band,V. and
            Band,H.
  TITLE     Ehd4 is required to attain normal prepubertal testis size but
            dispensable for fertility in male mice
  JOURNAL   Genesis 48 (5), 328-342 (2010)
   PUBMED   20213691
  REMARK    GeneRIF: Results suggest a role for EHD4 in the proper development
            of postmitotic and postmeiotic germ cells.
REFERENCE   7  (bases 1 to 3359)
  AUTHORS   Sengupta,S., George,M., Miller,K.K., Naik,K., Chou,J.,
            Cheatham,M.A., Dallos,P., Naramura,M., Band,H. and Zheng,J.
  TITLE     EHD4 and CDH23 are interacting partners in cochlear hair cells
  JOURNAL   J Biol Chem 284 (30), 20121-20129 (2009)
   PUBMED   19487694
  REMARK    GeneRIF: EHD4 is a novel CDH23-interacting protein that could
            regulate CDH23 trafficking/localization in a calcium-sensitive
            manner.
REFERENCE   8  (bases 1 to 3359)
  AUTHORS   Blume,J.J., Halbach,A., Behrendt,D., Paulsson,M. and Plomann,M.
  TITLE     EHD proteins are associated with tubular and vesicular compartments
            and interact with specific phospholipids
  JOURNAL   Exp Cell Res 313 (2), 219-231 (2007)
   PUBMED   17097635
REFERENCE   9  (bases 1 to 3359)
  AUTHORS   Naslavsky,N. and Caplan,S.
  TITLE     C-terminal EH-domain-containing proteins: consensus for a role in
            endocytic trafficking, EH?
  JOURNAL   J Cell Sci 118 (Pt 18), 4093-4101 (2005)
   PUBMED   16155252
REFERENCE   10 (bases 1 to 3359)
  AUTHORS   Xu,Y., Shi,H., Wei,S., Wong,S.H. and Hong,W.
  TITLE     Mutually exclusive interactions of EHD1 with GS32 and syndapin II
  JOURNAL   Mol Membr Biol 21 (4), 269-277 (2004)
   PUBMED   15371016
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833774.4.
            
            On Mar 24, 2023 this sequence version replaced NM_133838.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF173639.1, BC012272.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-308               AL833774.4         33484-33791
            309-485             AL833774.4         50956-51132
            486-583             AL833774.4         60342-60439
            584-996             AL833774.4         85599-86011
            997-1161            AL833774.4         90904-91068
            1162-3359           AL833774.4         96347-98544
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.16 cM"
     gene            1..3359
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /note="EH-domain containing 4"
                     /db_xref="GeneID:98878"
                     /db_xref="MGI:MGI:1919619"
     exon            1..308
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..1698
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /note="isoform 1 is encoded by transcript variant 1; EH
                     domain-containing protein 4; EH domain containing protein
                     MPAST2; mPAST2; PAST homolog 2"
                     /codon_start=1
                     /product="EH domain-containing protein 4 isoform 1"
                     /protein_id="NP_598599.2"
                     /db_xref="CCDS:CCDS16615.1"
                     /db_xref="GeneID:98878"
                     /db_xref="MGI:MGI:1919619"
                     /translation="MFSWMGRQAGGRERSGGMDAVQTVTGGLRSLYQRKVLPLEEAYR
                     FHEFHSPALEDADFENKPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIA
                     VMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISIIDSPGI
                     LSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIR
                     VVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEA
                     EAQDLFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPNMFGKENKKREL
                     IYRLPEIYVQLQREYQISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLTNK
                     ISSLMGLISQEEMNMPTQMVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPV
                     YDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAK
                     HLIKIKLDGYELPNSLPPHLVPPSHRKSLPKAD"
     exon            309..485
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /inference="alignment:Splign:2.1.0"
     exon            486..583
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /inference="alignment:Splign:2.1.0"
     exon            584..996
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1161
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..3359
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3342..3347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /note="hexamer: AATATA"
     polyA_site      3359
                     /gene="Ehd4"
                     /gene_synonym="2210022F10Rik; Past2"
                     /note="major polyA site"
ORIGIN      
        1 gagcgagcga cgcgggttct ggggttctta ctgaagtgcg gcggcggcgg cggcaagttg
       61 tggggcggca gcatgttcag ctggatgggg cggcaggctg gcgggcgcga gcgctcgggc
      121 ggcatggacg cggtgcagac ggtgacgggc ggcctgcgct ctctgtacca gcgcaaggtg
      181 ctgccgctgg aggaggcgta ccgcttccac gagtttcact cgccagcgct ggaggacgcc
      241 gacttcgaaa acaagcccat gatcctgctg gtgggccagt acagcacggg caagaccacg
      301 ttcatcagat acttactgga gcaagatttc ccaggcatga gaattggtcc agagccaacc
      361 acagattcct tcattgctgt gatgtatggg gagactgagg gaagcacccc tgggaatgct
      421 ttagttgtgg atcccaaaaa gccattccga aagcttagtc gctttggaaa tgctttcctg
      481 aacagattca tgtgctcaca gctgcccaac caagttctga agagcatcag catcatcgac
      541 agtcctggca ttctgtctgg ggagaagcag cgcataagcc gagggtatga cttctgccag
      601 gtcttacagt ggtttgccga gagggttgac aggatcatcc tgctctttga cgctcacaaa
      661 ttggacatct cagatgagtt ctcagaggcg atcaaggcct tccggggcca ggatgacaaa
      721 atccgagtgg tactgaacaa ggctgaccaa gtggatacac agcagctgat gcgagtctat
      781 ggggccctca tgtggtctct gggcaaggtc atcaacacac ctgaagtact tcgagtctac
      841 attggttcat tttgggcaca gcccctgcaa aacaccgaca accgccggct ctttgaggct
      901 gaagcacagg acctcttccg agacatccag agcctgcccc agaaggctgc agtgcgcaaa
      961 ctcaatgacc tcatcaagcg tgcaaggttg gccaaagtcc acgcctacat catcagctac
     1021 ctgaagaagg aaatgccaaa tatgtttgga aaagaaaata agaagcgaga acttatctac
     1081 aggctcccgg aaatctatgt tcagctgcag cgagaatacc agatttctgc aggggacttc
     1141 cctgaggtca aggccatgca ggagcagctg gagaactacg acttcaccaa gttccactca
     1201 ctgaagccca agctgatcga ggctgtggac aacatgctga ccaacaagat ctcatccctg
     1261 atgggcctca tcagccagga ggagatgaac atgcccacgc agatggtgca gggcggcgcc
     1321 tttgatggca ccacagaggg gcccttcaac cagggctatg gggagggggc caaggagggg
     1381 gccgatgagg aagagtgggt cgtggctaaa gacaagccgg tgtatgacga actcttttac
     1441 acgctgtcac ccatcaatgg caagatatcg ggtgtcaacg ccaagaagga gatggtgacc
     1501 tccaagctgc ccaacagtgt cctgggcaag atctggaagc tggccgactg cgactgcgac
     1561 ggcatgctag acgaggagga gttcgcgctg gccaagcacc tcatcaagat caagctggac
     1621 ggctacgagc tgcccaacag cctgccccca cacctggtgc ccccctccca caggaagtcc
     1681 ctgccaaagg ctgactgagg gcccacagct ggggtgggag gggtggtgcc tgggcctgaa
     1741 gtctgctctg ccactgactc gctgaatgtc cgtggccaaa gttccgtcct ctccgtgact
     1801 cggttttctc atatgtaggg cagggagggc agatcccgga cactagatga ggtctttatg
     1861 cctctaaaat cctgaagttt ctatcaaaat attgagaaga gcagtatata gatatggaga
     1921 tcaaagaata agaagaaaaa aaaattatcc aaagaagaat gtaggtgtga cacaggctgt
     1981 cgaagtctgg gcactcaggg attgagccag ggactcactg gagccatcag agctgtgctg
     2041 gacagtaagt taccatgagc ctctttgtcc ttcagggggc actgagaggc agcaaaggac
     2101 ctaatttatt tttcttactg tgtttcctgc tctatctaga gaggaaaagg aacagaacag
     2161 agagtttctg ccttccatag attaaaaacc aaagagaaga ctaataccca ggtttgaaag
     2221 aggatgtgcc atgtctgtgt gggcccatct gcagtcccca tcagagtagg cagccaggca
     2281 aggagaacct gtcttgtccc agcacctgct gcctgaccct gccaagcctc cctcagttag
     2341 gaattcataa ttcacaaact gtcagcccct ggcaagacct gtgtgccctt caaccactga
     2401 atgtccccaa agcttttttg gtaggtggca gaatttaaat tcaataaagc ttgtcttgat
     2461 ttgtgtggag tcccagagcc ttcggaccca ggtaaagcga gagcagcccc atcctgagct
     2521 gtgagtggaa gagaccagcg ccctggtgat tgatgatgtt gtttgatttt taaagaaaga
     2581 aatgcatttc attgtgtcca ggtatgtttt tctcacaaga tgtagcctct ctgaagaaat
     2641 ctctgtaata ccttttcttc caagctagac acctcacatc aggagtgcct atctgggcca
     2701 ggattggtct acttctgcct cttcagtgtt ctttcccaag cccacctagt caggcgccgc
     2761 aggggctgcc tcctactgta ccctgctctg tggcccagca gaagaacact gtcaccatca
     2821 cttacgaagt aggaatcaca gcgcagcagt gaccacatga ccaacagcag cagaaatgaa
     2881 tgaatctgaa cagcatccct gaatcttcac aaaaacatac tccaatccct aattgtctgc
     2941 atctggcaac ctcagggtca gaaccgggga tttaagagaa aatgctaaga gctcacaccc
     3001 atttcgcagc acttgggagg cagatgcagg gggattacca tgagttcgaa gccagtctga
     3061 actacatcgt ttgttttatg gtagtctgtg taaaacagaa tacttacgcc accattcaca
     3121 cttcctatct tactttctaa ataatggttt taagagaaac acagtgtttg ctttactctg
     3181 aaagcgtgtt tttattaatt ggacacacac aggtgaatgt ttgatcccgg ggctgtaact
     3241 gtatttgtgt cagtgtccat ggagaggtga tgcctgggag gtcttgactt tcctcctagt
     3301 tgtcttctgc tttctctgca cagttgtcat atatctacct aaatataatc acaaatgta
//
