LOCUS       NM_153068               5029 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus EH-domain containing 2 (Ehd2), transcript variant 1,
            mRNA.
ACCESSION   NM_153068 XM_983037
VERSION     NM_153068.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Neuhaus,M., Fryklund,C., Taylor,H., Borreguero-Munoz,A.,
            Kopietz,F., Ardalani,H., Rogova,O., Stirrat,L., Bremner,S.K.,
            Spegel,P., Bryant,N.J., Gould,G.W. and Stenkula,K.G.
  TITLE     EHD2 regulates plasma membrane integrity and downstream insulin
            receptor signaling events
  JOURNAL   Mol Biol Cell 34 (12), ar124 (2023)
   PUBMED   37703099
  REMARK    GeneRIF: EHD2 regulates plasma membrane integrity and downstream
            insulin receptor signaling events.
REFERENCE   3  (bases 1 to 5029)
  AUTHORS   Luan,H., Bielecki,T.A., Mohapatra,B.C., Islam,N., Mushtaq,I.,
            Bhat,A.M., Mirza,S., Chakraborty,S., Raza,M., Storck,M.D.,
            Toss,M.S., Meza,J.L., Thoreson,W.B., Coulter,D.W., Rakha,E.A.,
            Band,V. and Band,H.
  TITLE     EHD2 overexpression promotes tumorigenesis and metastasis in
            triple-negative breast cancer by regulating store-operated calcium
            entry
  JOURNAL   Elife 12, e81288 (2023)
   PUBMED   36625722
  REMARK    GeneRIF: EHD2 overexpression promotes tumorigenesis and metastasis
            in triple-negative breast cancer by regulating store-operated
            calcium entry.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5029)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   5  (bases 1 to 5029)
  AUTHORS   Fryklund,C., Moren,B., Shah,S., Grossi,M., Degerman,E.,
            Matthaeus,C. and Stenkula,K.G.
  TITLE     EH Domain-Containing 2 Deficiency Restricts Adipose Tissue
            Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes
  JOURNAL   Front Physiol 12, 740666 (2021)
   PUBMED   34630160
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5029)
  AUTHORS   Braun,A., Pinyol,R., Dahlhaus,R., Koch,D., Fonarev,P., Grant,B.D.,
            Kessels,M.M. and Qualmann,B.
  TITLE     EHD proteins associate with syndapin I and II and such interactions
            play a crucial role in endosomal recycling
  JOURNAL   Mol Biol Cell 16 (8), 3642-3658 (2005)
   PUBMED   15930129
REFERENCE   7  (bases 1 to 5029)
  AUTHORS   Guilherme,A., Soriano,N.A., Furcinitti,P.S. and Czech,M.P.
  TITLE     Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated
            GLUT4 recycling in 3T3-L1 adipocytes
  JOURNAL   J Biol Chem 279 (38), 40062-40075 (2004)
   PUBMED   15247266
REFERENCE   8  (bases 1 to 5029)
  AUTHORS   Guilherme,A., Soriano,N.A., Bose,S., Holik,J., Bose,A.,
            Pomerleau,D.P., Furcinitti,P., Leszyk,J., Corvera,S. and Czech,M.P.
  TITLE     EHD2 and the novel EH domain binding protein EHBP1 couple
            endocytosis to the actin cytoskeleton
  JOURNAL   J Biol Chem 279 (11), 10593-10605 (2004)
   PUBMED   14676205
  REMARK    GeneRIF: appears to connect endocytosis to the actin cytoskeleton
            through interactions of its N-terminal domain with membranes and
            its C-terminal EH domain with the novel EHBP1 protein
REFERENCE   9  (bases 1 to 5029)
  AUTHORS   Hong,H.K., Chakravarti,A. and Takahashi,J.S.
  TITLE     The gene for soluble N-ethylmaleimide sensitive factor attachment
            protein alpha is mutated in hydrocephaly with hop gait (hyh) mice
  JOURNAL   Proc Natl Acad Sci U S A 101 (6), 1748-1753 (2004)
   PUBMED   14755058
REFERENCE   10 (bases 1 to 5029)
  AUTHORS   Chae,T.H., Allen,K.M., Davisson,M.T., Sweet,H.O. and Walsh,C.A.
  TITLE     Mapping of the mouse hyh gene to a YAC/BAC contig on proximal
            Chromosome 7
  JOURNAL   Mamm Genome 13 (5), 239-244 (2002)
   PUBMED   12016511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC148990.6.
            
            On Mar 31, 2023 this sequence version replaced NM_153068.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046566.1, AK084455.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AC148990.6         149347-149404       c
            59-518              AC148990.6         145834-146293       c
            519-616             AC148990.6         145307-145404       c
            617-1029            AC148990.6         139418-139830       c
            1030-1194           AC148990.6         133993-134157       c
            1195-5029           AC148990.6         128885-132719       c
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 8.65 cM"
     gene            1..5029
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="EH-domain containing 2"
                     /db_xref="GeneID:259300"
                     /db_xref="MGI:MGI:2154274"
     exon            1..58
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            59..518
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..1746
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="isoform 1 is encoded by transcript variant 1; EH
                     domain-containing protein 2"
                     /codon_start=1
                     /product="EH domain-containing protein 2 isoform 1"
                     /protein_id="NP_694708.2"
                     /db_xref="CCDS:CCDS20842.1"
                     /db_xref="GeneID:259300"
                     /db_xref="MGI:MGI:2154274"
                     /translation="MFSWLKKGGARGQRPEAIRTVTSSLKELYRTKLLPLEEHYRFGS
                     FHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMH
                     GETEGTVPGNALVVDPEKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSG
                     AKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVL
                     NKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQ
                     DLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPTVFGKENKKKQLILK
                     LPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDDMLAQDIAK
                     LMPLLRQEELESVEAGVQGGAFEGTRMGPFVERGPDEAIEDGEEGSEDDAEWVVTKDK
                     SKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFAL
                     ASHLIEAKLEGHGLPTNLPRRLVPPSKRRQKGSAE"
     exon            519..616
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            617..1029
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1194
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..5029
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2979..2984
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3001
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="major polyA site"
     regulatory      5009..5014
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5029
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
ORIGIN      
        1 actccactgc ggacaggaca gagctcgggc tgcttgctct gcgctcccca gatccctggt
       61 gagctctcca ccttgtagtc tccttgggtg acccagtggg catcgaaagt caccatgttc
      121 agctggctga agaagggcgg ggcccggggc cagcgacctg aggccatccg caccgtgacc
      181 tcatccctca aggagctgta tcgcacgaaa cttcttcccc tggaggaaca ctaccgtttt
      241 gggtctttcc attcaccggc ccttgaagat gctgacttcg acggcaaacc catggtgctg
      301 gtggccggcc agtatagcac cggcaaaacc agcttcatcc agtaccttct ggagcaggaa
      361 gtccctggct cccgcgtggg tcccgagccc accaccgact gcttcgtggc tgtcatgcat
      421 ggcgagacag agggcaccgt gcccggaaat gctctcgttg tggacccaga gaagcccttc
      481 cgcaaactca acccttttgg aaacactttt ctcaacaggt ttatgtgcgc ccaactcccc
      541 aaccaggtcc tggagagcat cagcatcatc gacacgcctg gcatcctatc aggtgcaaaa
      601 cagagagtga gccgtggcta cgacttccca gctgtcctgc gctggtttgc tgaacgcgtg
      661 gacctcatca tcttgctctt tgatgcccac aagctggaga tttccgatga attctcggag
      721 gccatcggtg ccttgcgtgg ccacgaggat aagatccgcg tggtactcaa caaggcagac
      781 atggtggaga cacagcagct gatgcgcgtc tacggggcgc tcatgtgggc gctgggcaag
      841 gtggtgggca cgcctgaggt ccttcgcgtc tacattggct ccttctggtc ccagccgctc
      901 cttgtgccgg acaaccggcg tcttttcgag ctggaggaac aggacctctt ccgtgatatc
      961 caaggcctac cgcgccatgc ggcgctgcgc aagctcaacg acctagtgaa acgggcgcgg
     1021 ctggtgcggg tacacgctta catcatcagc tacctgaaga aagagatgcc cacggtgttc
     1081 gggaaggaaa acaagaagaa gcagctgatc ctgaagctgc ctgtcatctt tgcgaagatc
     1141 cagctggagc accacatctc ccctggagac tttcctgact gccagaagat gcaggagctg
     1201 ttgatggcac atgacttcac caagttccac tcgctaaagc cgaagctgct ggaggcgcta
     1261 gatgacatgc tggcccaaga cattgccaag cttatgccgc tgcttcggca ggaggagctg
     1321 gagagtgtcg aggctggagt gcagggcggt gcgtttgagg gcacccgcat gggccccttc
     1381 gtggagcggg gccctgatga agcaatagag gatggtgagg agggctcaga agatgatgcc
     1441 gaatgggtgg tgaccaagga caagtccaaa tacgatgaaa tcttctacaa cttagctcct
     1501 gctgatggca agctgagtgg ctctaaggcc aaaacctgga tggtgggcac aaagctaccc
     1561 aactctgtgc tggggagaat ctggaagctg agcgatgtcg accgagacgg catgctggat
     1621 gatgaggagt ttgccttggc cagccacctc attgaggcta agctcgaagg ccatggtcta
     1681 cccaccaacc taccccgccg cctggtgccg ccttctaagc gacgacagaa gggctctgct
     1741 gaatgagaca gcagcgactg ctggctcatc ctccctcttt gccctgctgt ggctcctccc
     1801 cagctccagt tggctacacg gctacacgcg gatccctgct ctggcccact caccctgcct
     1861 gcctaccctg cctggctata aggacttgag cggggagatt ctctcccaca ccagacattc
     1921 agtgaaagca cttatagagg accagggggg agtgacaagc cttctctgtc agtccttcac
     1981 acctgcacct tcacatacac ggagcttatc acagaaacat acacagacat ccatccatca
     2041 ttcatataaa gacctgtttt tttcctttta ttttttgaaa gagtctcaca taacctaggc
     2101 tacccttgaa cttcttatgt agctgaggat gaccttgaac tcttcatctt cctggctcca
     2161 cttctgcgtg ctggagttgc aggcatgaac caccatgcct ggtttgtgca gtcctggggt
     2221 atgtacccag cacttcctgc atactaggca agcactctag caactgagct acatccacag
     2281 ccccatccag atatttattg agcacttact gtatgctggg ccctggtgga accgactctc
     2341 aggagtgtga catctacaca gtcacacaaa cactgatacc agcaagaccc ccttttatag
     2401 ttaagcagct actgttactc agccatgcta gggctcccta ctcttttatg tcttcaggtg
     2461 tcaggctcac tggtggttca gagcagacac tcagcttcct cgctcctctc atgaacctct
     2521 ctttcgcttt cctacccaca aatcctaaat tctgaccaca tccacttcac aaagattgtg
     2581 cagcagatag aaaaaccgca acttcctggc tccttcgggg atggtttcca ttccccagag
     2641 cccatggttt ccccagcgtg tggggttggg aaccaaagta tctggcccct ctgcccacct
     2701 tcaggtccta tttaagtaaa gtccctatca aagaccgaga ccatggggtg ggaataggag
     2761 atgctttacc aaagcccagg ttttcatgga tatcctgggg cattgggaat tctcggtgct
     2821 atagcctctc ctcctctcta caggggcctc acacacttta tgtcttagca tccttagtac
     2881 ctgaccctgg tgggagaaaa aaataagatg gggcgggggg gggggcgttg aggacagcta
     2941 gaaggggctg aaaacccaag agtgtctcct acggggccaa taaagccaaa gctgctgctt
     3001 gtgtcgctgt caggctatct tggggcgggg acccctaagg aagaggtgcc tggggaacca
     3061 ttctggctca caggggcggt gcagtttgtg actcaactct gcgctgactc agtctcgggc
     3121 tgggggccag tcctgcatat tttccgaaga aaagttactc agggccctcc ccacccttgg
     3181 gacacccctc cctcccgctt tccgccttgg ggcctgggaa cccagttctg ggcgtggacg
     3241 aggaccagtg ccagtttgtg tacagtttgc ccttagtctc caaagcacct tccaacgcgc
     3301 actgacacga atcgaatgca ccagagcaga tcctgtagcc cgagaccact gggtcctgga
     3361 ggaggaggga ttagtgtgag agtgcacagg acactgatcc agcccacacc cggtgcgccc
     3421 gagacacttt ggcctggaga atctcccgcc cacgcacgcc ctgctccgca gccctgggcg
     3481 ccctctggtg gaaaggtagt gactcaggcc aggccatcac ctcaatatcc tgcccaggga
     3541 tgagctgagt tctactttga ccccggggag acatgttccc tccccgtgcc tccccacccc
     3601 ctcccccttt aggagtccat tttaattcca gcatgccaat ctgagcctgg ggcgggggtg
     3661 gggagcagtg tgtgctcaag tcattaatcc cagaacagag gcagaggcag gtggtggatc
     3721 tgtgaactgg agggcagcct ggtctacaga gtgagttcca ggacagctag ggctacaccg
     3781 agaaaccctg tatcgaaaga taaaggtgtg gtgtggctag ggctgtagtt aacagtactt
     3841 gccctgctta cacggggctc agtttcccac cttttgcatg acctgaaaac aaaacccttt
     3901 gtagatctct ttggatgact accactttcc aggggcctgg ggatggggga tgggtgtgga
     3961 ggcagtgata gttggctggc acttggatgc taggcaccaa tattctagat ttggaccttt
     4021 aaaactgagt tcatgcatgc acaggtctgt ggatcacaga tgtgatgggg tctggagatt
     4081 gaaacagggt cctctggaag agcagccagt gcttttaaca tctcttcagg ccacatactc
     4141 agcttttaat gataagttta accgctgaat aatctctcca ggccaattac tcagcctttt
     4201 ttttgttaag ttttgggaac ttaaattcag gtctttattg tttgcccaga aagctctctt
     4261 acccactgag ccattcccca acccttgttt ttcattttga gacagtctct ctaccctaaa
     4321 ctcattctgc tgtccagggt ggcctatgaa cttgccatcc tcctgcctca gtgcctcttg
     4381 agtaggtagg taaggttaaa aatgaacagc actacccagc tatttttttt tcttttagag
     4441 tgtgtgtgtg tgtgtgtgtg tgtgtgtttt atacaggcaa ttccattggc ctgctttcag
     4501 ggacccagcc agtgcttaca tcatgaccta cggcagccag atgtaccagt agatgtaagg
     4561 acccacctgc caccccagct ctctctctct gatctccacc accaatgttc cctctccttc
     4621 tctgtcccct ctctctgccc tcatgattct gccccccatc tgtctgcctg tctatctgtc
     4681 cacttgtctg cctctgtccc ttcaccaagt cccacagtac tcttgcgttc cccccaataa
     4741 acctttacac taggtctgtt ttatggtgta acttctcagg ggtacacctt ggccaggccc
     4801 tccaaaaata ctcccttgcc acattatatt tcataacggc gtgtaagcat atatgccacc
     4861 acagcatacc ttgtgaaggt caagaacaaa cagcttgcag gtattggctt tttccaacat
     4921 atgggttatg aggattaatc tgaggcttgg cagcaacagc ctttctctac tgaggtatct
     4981 tggcagcccc acacccagca tcttctttaa taaaaagagt taaagaaaa
//
