LOCUS       NM_001419313            5060 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus EH-domain containing 2 (Ehd2), transcript variant 3,
            mRNA.
ACCESSION   NM_001419313 XM_011250582
VERSION     NM_001419313.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Neuhaus,M., Fryklund,C., Taylor,H., Borreguero-Munoz,A.,
            Kopietz,F., Ardalani,H., Rogova,O., Stirrat,L., Bremner,S.K.,
            Spegel,P., Bryant,N.J., Gould,G.W. and Stenkula,K.G.
  TITLE     EHD2 regulates plasma membrane integrity and downstream insulin
            receptor signaling events
  JOURNAL   Mol Biol Cell 34 (12), ar124 (2023)
   PUBMED   37703099
  REMARK    GeneRIF: EHD2 regulates plasma membrane integrity and downstream
            insulin receptor signaling events.
REFERENCE   3  (bases 1 to 5060)
  AUTHORS   Luan,H., Bielecki,T.A., Mohapatra,B.C., Islam,N., Mushtaq,I.,
            Bhat,A.M., Mirza,S., Chakraborty,S., Raza,M., Storck,M.D.,
            Toss,M.S., Meza,J.L., Thoreson,W.B., Coulter,D.W., Rakha,E.A.,
            Band,V. and Band,H.
  TITLE     EHD2 overexpression promotes tumorigenesis and metastasis in
            triple-negative breast cancer by regulating store-operated calcium
            entry
  JOURNAL   Elife 12, e81288 (2023)
   PUBMED   36625722
  REMARK    GeneRIF: EHD2 overexpression promotes tumorigenesis and metastasis
            in triple-negative breast cancer by regulating store-operated
            calcium entry.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5060)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   5  (bases 1 to 5060)
  AUTHORS   Fryklund,C., Moren,B., Shah,S., Grossi,M., Degerman,E.,
            Matthaeus,C. and Stenkula,K.G.
  TITLE     EH Domain-Containing 2 Deficiency Restricts Adipose Tissue
            Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes
  JOURNAL   Front Physiol 12, 740666 (2021)
   PUBMED   34630160
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5060)
  AUTHORS   Braun,A., Pinyol,R., Dahlhaus,R., Koch,D., Fonarev,P., Grant,B.D.,
            Kessels,M.M. and Qualmann,B.
  TITLE     EHD proteins associate with syndapin I and II and such interactions
            play a crucial role in endosomal recycling
  JOURNAL   Mol Biol Cell 16 (8), 3642-3658 (2005)
   PUBMED   15930129
REFERENCE   7  (bases 1 to 5060)
  AUTHORS   Guilherme,A., Soriano,N.A., Furcinitti,P.S. and Czech,M.P.
  TITLE     Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated
            GLUT4 recycling in 3T3-L1 adipocytes
  JOURNAL   J Biol Chem 279 (38), 40062-40075 (2004)
   PUBMED   15247266
REFERENCE   8  (bases 1 to 5060)
  AUTHORS   Guilherme,A., Soriano,N.A., Bose,S., Holik,J., Bose,A.,
            Pomerleau,D.P., Furcinitti,P., Leszyk,J., Corvera,S. and Czech,M.P.
  TITLE     EHD2 and the novel EH domain binding protein EHBP1 couple
            endocytosis to the actin cytoskeleton
  JOURNAL   J Biol Chem 279 (11), 10593-10605 (2004)
   PUBMED   14676205
  REMARK    GeneRIF: appears to connect endocytosis to the actin cytoskeleton
            through interactions of its N-terminal domain with membranes and
            its C-terminal EH domain with the novel EHBP1 protein
REFERENCE   9  (bases 1 to 5060)
  AUTHORS   Hong,H.K., Chakravarti,A. and Takahashi,J.S.
  TITLE     The gene for soluble N-ethylmaleimide sensitive factor attachment
            protein alpha is mutated in hydrocephaly with hop gait (hyh) mice
  JOURNAL   Proc Natl Acad Sci U S A 101 (6), 1748-1753 (2004)
   PUBMED   14755058
REFERENCE   10 (bases 1 to 5060)
  AUTHORS   Chae,T.H., Allen,K.M., Davisson,M.T., Sweet,H.O. and Walsh,C.A.
  TITLE     Mapping of the mouse hyh gene to a YAC/BAC contig on proximal
            Chromosome 7
  JOURNAL   Mamm Genome 13 (5), 239-244 (2002)
   PUBMED   12016511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC148990.6.
            
            On Mar 30, 2023 this sequence version replaced XM_011250582.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219380.72005.1,
                                           SRR13422598.573672.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AC148990.6         149347-149404       c
            59-549              AC148990.6         145834-146324       c
            550-647             AC148990.6         145307-145404       c
            648-1060            AC148990.6         139418-139830       c
            1061-1225           AC148990.6         133993-134157       c
            1226-5060           AC148990.6         128885-132719       c
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 8.65 cM"
     gene            1..5060
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="EH-domain containing 2"
                     /db_xref="GeneID:259300"
                     /db_xref="MGI:MGI:2154274"
     exon            1..58
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            59..549
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1777
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="isoform 1 is encoded by transcript variant 3; EH
                     domain-containing protein 2"
                     /codon_start=1
                     /product="EH domain-containing protein 2 isoform 1"
                     /protein_id="NP_001406242.1"
                     /db_xref="GeneID:259300"
                     /db_xref="MGI:MGI:2154274"
                     /translation="MFSWLKKGGARGQRPEAIRTVTSSLKELYRTKLLPLEEHYRFGS
                     FHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMH
                     GETEGTVPGNALVVDPEKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSG
                     AKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVL
                     NKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQ
                     DLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPTVFGKENKKKQLILK
                     LPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDDMLAQDIAK
                     LMPLLRQEELESVEAGVQGGAFEGTRMGPFVERGPDEAIEDGEEGSEDDAEWVVTKDK
                     SKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFAL
                     ASHLIEAKLEGHGLPTNLPRRLVPPSKRRQKGSAE"
     exon            550..647
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            648..1060
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1225
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..5060
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3010..3015
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3032
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="major polyA site"
     regulatory      5040..5045
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5060
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
ORIGIN      
        1 actccactgc ggacaggaca gagctcgggc tgcttgctct gcgctcccca gatccctgtc
       61 ttccattgtg ttcccccctc ttctggcagg tgagctctcc accttgtagt ctccttgggt
      121 gacccagtgg gcatcgaaag tcaccatgtt cagctggctg aagaagggcg gggcccgggg
      181 ccagcgacct gaggccatcc gcaccgtgac ctcatccctc aaggagctgt atcgcacgaa
      241 acttcttccc ctggaggaac actaccgttt tgggtctttc cattcaccgg cccttgaaga
      301 tgctgacttc gacggcaaac ccatggtgct ggtggccggc cagtatagca ccggcaaaac
      361 cagcttcatc cagtaccttc tggagcagga agtccctggc tcccgcgtgg gtcccgagcc
      421 caccaccgac tgcttcgtgg ctgtcatgca tggcgagaca gagggcaccg tgcccggaaa
      481 tgctctcgtt gtggacccag agaagccctt ccgcaaactc aacccttttg gaaacacttt
      541 tctcaacagg tttatgtgcg cccaactccc caaccaggtc ctggagagca tcagcatcat
      601 cgacacgcct ggcatcctat caggtgcaaa acagagagtg agccgtggct acgacttccc
      661 agctgtcctg cgctggtttg ctgaacgcgt ggacctcatc atcttgctct ttgatgccca
      721 caagctggag atttccgatg aattctcgga ggccatcggt gccttgcgtg gccacgagga
      781 taagatccgc gtggtactca acaaggcaga catggtggag acacagcagc tgatgcgcgt
      841 ctacggggcg ctcatgtggg cgctgggcaa ggtggtgggc acgcctgagg tccttcgcgt
      901 ctacattggc tccttctggt cccagccgct ccttgtgccg gacaaccggc gtcttttcga
      961 gctggaggaa caggacctct tccgtgatat ccaaggccta ccgcgccatg cggcgctgcg
     1021 caagctcaac gacctagtga aacgggcgcg gctggtgcgg gtacacgctt acatcatcag
     1081 ctacctgaag aaagagatgc ccacggtgtt cgggaaggaa aacaagaaga agcagctgat
     1141 cctgaagctg cctgtcatct ttgcgaagat ccagctggag caccacatct cccctggaga
     1201 ctttcctgac tgccagaaga tgcaggagct gttgatggca catgacttca ccaagttcca
     1261 ctcgctaaag ccgaagctgc tggaggcgct agatgacatg ctggcccaag acattgccaa
     1321 gcttatgccg ctgcttcggc aggaggagct ggagagtgtc gaggctggag tgcagggcgg
     1381 tgcgtttgag ggcacccgca tgggcccctt cgtggagcgg ggccctgatg aagcaataga
     1441 ggatggtgag gagggctcag aagatgatgc cgaatgggtg gtgaccaagg acaagtccaa
     1501 atacgatgaa atcttctaca acttagctcc tgctgatggc aagctgagtg gctctaaggc
     1561 caaaacctgg atggtgggca caaagctacc caactctgtg ctggggagaa tctggaagct
     1621 gagcgatgtc gaccgagacg gcatgctgga tgatgaggag tttgccttgg ccagccacct
     1681 cattgaggct aagctcgaag gccatggtct acccaccaac ctaccccgcc gcctggtgcc
     1741 gccttctaag cgacgacaga agggctctgc tgaatgagac agcagcgact gctggctcat
     1801 cctccctctt tgccctgctg tggctcctcc ccagctccag ttggctacac ggctacacgc
     1861 ggatccctgc tctggcccac tcaccctgcc tgcctaccct gcctggctat aaggacttga
     1921 gcggggagat tctctcccac accagacatt cagtgaaagc acttatagag gaccaggggg
     1981 gagtgacaag ccttctctgt cagtccttca cacctgcacc ttcacataca cggagcttat
     2041 cacagaaaca tacacagaca tccatccatc attcatataa agacctgttt ttttcctttt
     2101 attttttgaa agagtctcac ataacctagg ctacccttga acttcttatg tagctgagga
     2161 tgaccttgaa ctcttcatct tcctggctcc acttctgcgt gctggagttg caggcatgaa
     2221 ccaccatgcc tggtttgtgc agtcctgggg tatgtaccca gcacttcctg catactaggc
     2281 aagcactcta gcaactgagc tacatccaca gccccatcca gatatttatt gagcacttac
     2341 tgtatgctgg gccctggtgg aaccgactct caggagtgtg acatctacac agtcacacaa
     2401 acactgatac cagcaagacc cccttttata gttaagcagc tactgttact cagccatgct
     2461 agggctccct actcttttat gtcttcaggt gtcaggctca ctggtggttc agagcagaca
     2521 ctcagcttcc tcgctcctct catgaacctc tctttcgctt tcctacccac aaatcctaaa
     2581 ttctgaccac atccacttca caaagattgt gcagcagata gaaaaaccgc aacttcctgg
     2641 ctccttcggg gatggtttcc attccccaga gcccatggtt tccccagcgt gtggggttgg
     2701 gaaccaaagt atctggcccc tctgcccacc ttcaggtcct atttaagtaa agtccctatc
     2761 aaagaccgag accatggggt gggaatagga gatgctttac caaagcccag gttttcatgg
     2821 atatcctggg gcattgggaa ttctcggtgc tatagcctct cctcctctct acaggggcct
     2881 cacacacttt atgtcttagc atccttagta cctgaccctg gtgggagaaa aaaataagat
     2941 ggggcggggg ggggggcgtt gaggacagct agaaggggct gaaaacccaa gagtgtctcc
     3001 tacggggcca ataaagccaa agctgctgct tgtgtcgctg tcaggctatc ttggggcggg
     3061 gacccctaag gaagaggtgc ctggggaacc attctggctc acaggggcgg tgcagtttgt
     3121 gactcaactc tgcgctgact cagtctcggg ctgggggcca gtcctgcata ttttccgaag
     3181 aaaagttact cagggccctc cccacccttg ggacacccct ccctcccgct ttccgccttg
     3241 gggcctggga acccagttct gggcgtggac gaggaccagt gccagtttgt gtacagtttg
     3301 cccttagtct ccaaagcacc ttccaacgcg cactgacacg aatcgaatgc accagagcag
     3361 atcctgtagc ccgagaccac tgggtcctgg aggaggaggg attagtgtga gagtgcacag
     3421 gacactgatc cagcccacac ccggtgcgcc cgagacactt tggcctggag aatctcccgc
     3481 ccacgcacgc cctgctccgc agccctgggc gccctctggt ggaaaggtag tgactcaggc
     3541 caggccatca cctcaatatc ctgcccaggg atgagctgag ttctactttg accccgggga
     3601 gacatgttcc ctccccgtgc ctccccaccc cctccccctt taggagtcca ttttaattcc
     3661 agcatgccaa tctgagcctg gggcgggggt ggggagcagt gtgtgctcaa gtcattaatc
     3721 ccagaacaga ggcagaggca ggtggtggat ctgtgaactg gagggcagcc tggtctacag
     3781 agtgagttcc aggacagcta gggctacacc gagaaaccct gtatcgaaag ataaaggtgt
     3841 ggtgtggcta gggctgtagt taacagtact tgccctgctt acacggggct cagtttccca
     3901 ccttttgcat gacctgaaaa caaaaccctt tgtagatctc tttggatgac taccactttc
     3961 caggggcctg gggatggggg atgggtgtgg aggcagtgat agttggctgg cacttggatg
     4021 ctaggcacca atattctaga tttggacctt taaaactgag ttcatgcatg cacaggtctg
     4081 tggatcacag atgtgatggg gtctggagat tgaaacaggg tcctctggaa gagcagccag
     4141 tgcttttaac atctcttcag gccacatact cagcttttaa tgataagttt aaccgctgaa
     4201 taatctctcc aggccaatta ctcagccttt tttttgttaa gttttgggaa cttaaattca
     4261 ggtctttatt gtttgcccag aaagctctct tacccactga gccattcccc aacccttgtt
     4321 tttcattttg agacagtctc tctaccctaa actcattctg ctgtccaggg tggcctatga
     4381 acttgccatc ctcctgcctc agtgcctctt gagtaggtag gtaaggttaa aaatgaacag
     4441 cactacccag ctattttttt ttcttttaga gtgtgtgtgt gtgtgtgtgt gtgtgtgttt
     4501 tatacaggca attccattgg cctgctttca gggacccagc cagtgcttac atcatgacct
     4561 acggcagcca gatgtaccag tagatgtaag gacccacctg ccaccccagc tctctctctc
     4621 tgatctccac caccaatgtt ccctctcctt ctctgtcccc tctctctgcc ctcatgattc
     4681 tgccccccat ctgtctgcct gtctatctgt ccacttgtct gcctctgtcc cttcaccaag
     4741 tcccacagta ctcttgcgtt ccccccaata aacctttaca ctaggtctgt tttatggtgt
     4801 aacttctcag gggtacacct tggccaggcc ctccaaaaat actcccttgc cacattatat
     4861 ttcataacgg cgtgtaagca tatatgccac cacagcatac cttgtgaagg tcaagaacaa
     4921 acagcttgca ggtattggct ttttccaaca tatgggttat gaggattaat ctgaggcttg
     4981 gcagcaacag cctttctcta ctgaggtatc ttggcagccc cacacccagc atcttcttta
     5041 ataaaaagag ttaaagaaaa
//
