LOCUS       NM_001419312            5023 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus EH-domain containing 2 (Ehd2), transcript variant 2,
            mRNA.
ACCESSION   NM_001419312
VERSION     NM_001419312.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Neuhaus,M., Fryklund,C., Taylor,H., Borreguero-Munoz,A.,
            Kopietz,F., Ardalani,H., Rogova,O., Stirrat,L., Bremner,S.K.,
            Spegel,P., Bryant,N.J., Gould,G.W. and Stenkula,K.G.
  TITLE     EHD2 regulates plasma membrane integrity and downstream insulin
            receptor signaling events
  JOURNAL   Mol Biol Cell 34 (12), ar124 (2023)
   PUBMED   37703099
  REMARK    GeneRIF: EHD2 regulates plasma membrane integrity and downstream
            insulin receptor signaling events.
REFERENCE   3  (bases 1 to 5023)
  AUTHORS   Luan,H., Bielecki,T.A., Mohapatra,B.C., Islam,N., Mushtaq,I.,
            Bhat,A.M., Mirza,S., Chakraborty,S., Raza,M., Storck,M.D.,
            Toss,M.S., Meza,J.L., Thoreson,W.B., Coulter,D.W., Rakha,E.A.,
            Band,V. and Band,H.
  TITLE     EHD2 overexpression promotes tumorigenesis and metastasis in
            triple-negative breast cancer by regulating store-operated calcium
            entry
  JOURNAL   Elife 12, e81288 (2023)
   PUBMED   36625722
  REMARK    GeneRIF: EHD2 overexpression promotes tumorigenesis and metastasis
            in triple-negative breast cancer by regulating store-operated
            calcium entry.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5023)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   5  (bases 1 to 5023)
  AUTHORS   Fryklund,C., Moren,B., Shah,S., Grossi,M., Degerman,E.,
            Matthaeus,C. and Stenkula,K.G.
  TITLE     EH Domain-Containing 2 Deficiency Restricts Adipose Tissue
            Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes
  JOURNAL   Front Physiol 12, 740666 (2021)
   PUBMED   34630160
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5023)
  AUTHORS   Braun,A., Pinyol,R., Dahlhaus,R., Koch,D., Fonarev,P., Grant,B.D.,
            Kessels,M.M. and Qualmann,B.
  TITLE     EHD proteins associate with syndapin I and II and such interactions
            play a crucial role in endosomal recycling
  JOURNAL   Mol Biol Cell 16 (8), 3642-3658 (2005)
   PUBMED   15930129
REFERENCE   7  (bases 1 to 5023)
  AUTHORS   Guilherme,A., Soriano,N.A., Furcinitti,P.S. and Czech,M.P.
  TITLE     Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated
            GLUT4 recycling in 3T3-L1 adipocytes
  JOURNAL   J Biol Chem 279 (38), 40062-40075 (2004)
   PUBMED   15247266
REFERENCE   8  (bases 1 to 5023)
  AUTHORS   Guilherme,A., Soriano,N.A., Bose,S., Holik,J., Bose,A.,
            Pomerleau,D.P., Furcinitti,P., Leszyk,J., Corvera,S. and Czech,M.P.
  TITLE     EHD2 and the novel EH domain binding protein EHBP1 couple
            endocytosis to the actin cytoskeleton
  JOURNAL   J Biol Chem 279 (11), 10593-10605 (2004)
   PUBMED   14676205
  REMARK    GeneRIF: appears to connect endocytosis to the actin cytoskeleton
            through interactions of its N-terminal domain with membranes and
            its C-terminal EH domain with the novel EHBP1 protein
REFERENCE   9  (bases 1 to 5023)
  AUTHORS   Hong,H.K., Chakravarti,A. and Takahashi,J.S.
  TITLE     The gene for soluble N-ethylmaleimide sensitive factor attachment
            protein alpha is mutated in hydrocephaly with hop gait (hyh) mice
  JOURNAL   Proc Natl Acad Sci U S A 101 (6), 1748-1753 (2004)
   PUBMED   14755058
REFERENCE   10 (bases 1 to 5023)
  AUTHORS   Chae,T.H., Allen,K.M., Davisson,M.T., Sweet,H.O. and Walsh,C.A.
  TITLE     Mapping of the mouse hyh gene to a YAC/BAC contig on proximal
            Chromosome 7
  JOURNAL   Mamm Genome 13 (5), 239-244 (2002)
   PUBMED   12016511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC148990.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253014.829423.1,
                                           SRR17253011.584462.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AC148990.6         149347-149404       c
            59-518              AC148990.6         145834-146293       c
            519-616             AC148990.6         145307-145404       c
            617-1023            AC148990.6         139424-139830       c
            1024-1188           AC148990.6         133993-134157       c
            1189-5023           AC148990.6         128885-132719       c
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 8.65 cM"
     gene            1..5023
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="EH-domain containing 2"
                     /db_xref="GeneID:259300"
                     /db_xref="MGI:MGI:2154274"
     exon            1..58
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            59..518
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..1740
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="isoform 2 is encoded by transcript variant 2; EH
                     domain-containing protein 2"
                     /codon_start=1
                     /product="EH domain-containing protein 2 isoform 2"
                     /protein_id="NP_001406241.1"
                     /db_xref="GeneID:259300"
                     /db_xref="MGI:MGI:2154274"
                     /translation="MFSWLKKGGARGQRPEAIRTVTSSLKELYRTKLLPLEEHYRFGS
                     FHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMH
                     GETEGTVPGNALVVDPEKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSG
                     AKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVL
                     NKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQ
                     DLFRDIQGLPRHAALRKLNDLVKRARLVHAYIISYLKKEMPTVFGKENKKKQLILKLP
                     VIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDDMLAQDIAKLM
                     PLLRQEELESVEAGVQGGAFEGTRMGPFVERGPDEAIEDGEEGSEDDAEWVVTKDKSK
                     YDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFALAS
                     HLIEAKLEGHGLPTNLPRRLVPPSKRRQKGSAE"
     exon            519..616
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            617..1023
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1188
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..5023
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2973..2978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2995
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="major polyA site"
     regulatory      5003..5008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5023
                     /gene="Ehd2"
                     /gene_synonym="C130052H20Rik"
ORIGIN      
        1 actccactgc ggacaggaca gagctcgggc tgcttgctct gcgctcccca gatccctggt
       61 gagctctcca ccttgtagtc tccttgggtg acccagtggg catcgaaagt caccatgttc
      121 agctggctga agaagggcgg ggcccggggc cagcgacctg aggccatccg caccgtgacc
      181 tcatccctca aggagctgta tcgcacgaaa cttcttcccc tggaggaaca ctaccgtttt
      241 gggtctttcc attcaccggc ccttgaagat gctgacttcg acggcaaacc catggtgctg
      301 gtggccggcc agtatagcac cggcaaaacc agcttcatcc agtaccttct ggagcaggaa
      361 gtccctggct cccgcgtggg tcccgagccc accaccgact gcttcgtggc tgtcatgcat
      421 ggcgagacag agggcaccgt gcccggaaat gctctcgttg tggacccaga gaagcccttc
      481 cgcaaactca acccttttgg aaacactttt ctcaacaggt ttatgtgcgc ccaactcccc
      541 aaccaggtcc tggagagcat cagcatcatc gacacgcctg gcatcctatc aggtgcaaaa
      601 cagagagtga gccgtggcta cgacttccca gctgtcctgc gctggtttgc tgaacgcgtg
      661 gacctcatca tcttgctctt tgatgcccac aagctggaga tttccgatga attctcggag
      721 gccatcggtg ccttgcgtgg ccacgaggat aagatccgcg tggtactcaa caaggcagac
      781 atggtggaga cacagcagct gatgcgcgtc tacggggcgc tcatgtgggc gctgggcaag
      841 gtggtgggca cgcctgaggt ccttcgcgtc tacattggct ccttctggtc ccagccgctc
      901 cttgtgccgg acaaccggcg tcttttcgag ctggaggaac aggacctctt ccgtgatatc
      961 caaggcctac cgcgccatgc ggcgctgcgc aagctcaacg acctagtgaa acgggcgcgg
     1021 ctggtacacg cttacatcat cagctacctg aagaaagaga tgcccacggt gttcgggaag
     1081 gaaaacaaga agaagcagct gatcctgaag ctgcctgtca tctttgcgaa gatccagctg
     1141 gagcaccaca tctcccctgg agactttcct gactgccaga agatgcagga gctgttgatg
     1201 gcacatgact tcaccaagtt ccactcgcta aagccgaagc tgctggaggc gctagatgac
     1261 atgctggccc aagacattgc caagcttatg ccgctgcttc ggcaggagga gctggagagt
     1321 gtcgaggctg gagtgcaggg cggtgcgttt gagggcaccc gcatgggccc cttcgtggag
     1381 cggggccctg atgaagcaat agaggatggt gaggagggct cagaagatga tgccgaatgg
     1441 gtggtgacca aggacaagtc caaatacgat gaaatcttct acaacttagc tcctgctgat
     1501 ggcaagctga gtggctctaa ggccaaaacc tggatggtgg gcacaaagct acccaactct
     1561 gtgctgggga gaatctggaa gctgagcgat gtcgaccgag acggcatgct ggatgatgag
     1621 gagtttgcct tggccagcca cctcattgag gctaagctcg aaggccatgg tctacccacc
     1681 aacctacccc gccgcctggt gccgccttct aagcgacgac agaagggctc tgctgaatga
     1741 gacagcagcg actgctggct catcctccct ctttgccctg ctgtggctcc tccccagctc
     1801 cagttggcta cacggctaca cgcggatccc tgctctggcc cactcaccct gcctgcctac
     1861 cctgcctggc tataaggact tgagcgggga gattctctcc cacaccagac attcagtgaa
     1921 agcacttata gaggaccagg ggggagtgac aagccttctc tgtcagtcct tcacacctgc
     1981 accttcacat acacggagct tatcacagaa acatacacag acatccatcc atcattcata
     2041 taaagacctg tttttttcct tttatttttt gaaagagtct cacataacct aggctaccct
     2101 tgaacttctt atgtagctga ggatgacctt gaactcttca tcttcctggc tccacttctg
     2161 cgtgctggag ttgcaggcat gaaccaccat gcctggtttg tgcagtcctg gggtatgtac
     2221 ccagcacttc ctgcatacta ggcaagcact ctagcaactg agctacatcc acagccccat
     2281 ccagatattt attgagcact tactgtatgc tgggccctgg tggaaccgac tctcaggagt
     2341 gtgacatcta cacagtcaca caaacactga taccagcaag accccctttt atagttaagc
     2401 agctactgtt actcagccat gctagggctc cctactcttt tatgtcttca ggtgtcaggc
     2461 tcactggtgg ttcagagcag acactcagct tcctcgctcc tctcatgaac ctctctttcg
     2521 ctttcctacc cacaaatcct aaattctgac cacatccact tcacaaagat tgtgcagcag
     2581 atagaaaaac cgcaacttcc tggctccttc ggggatggtt tccattcccc agagcccatg
     2641 gtttccccag cgtgtggggt tgggaaccaa agtatctggc ccctctgccc accttcaggt
     2701 cctatttaag taaagtccct atcaaagacc gagaccatgg ggtgggaata ggagatgctt
     2761 taccaaagcc caggttttca tggatatcct ggggcattgg gaattctcgg tgctatagcc
     2821 tctcctcctc tctacagggg cctcacacac tttatgtctt agcatcctta gtacctgacc
     2881 ctggtgggag aaaaaaataa gatggggcgg gggggggggc gttgaggaca gctagaaggg
     2941 gctgaaaacc caagagtgtc tcctacgggg ccaataaagc caaagctgct gcttgtgtcg
     3001 ctgtcaggct atcttggggc ggggacccct aaggaagagg tgcctgggga accattctgg
     3061 ctcacagggg cggtgcagtt tgtgactcaa ctctgcgctg actcagtctc gggctggggg
     3121 ccagtcctgc atattttccg aagaaaagtt actcagggcc ctccccaccc ttgggacacc
     3181 cctccctccc gctttccgcc ttggggcctg ggaacccagt tctgggcgtg gacgaggacc
     3241 agtgccagtt tgtgtacagt ttgcccttag tctccaaagc accttccaac gcgcactgac
     3301 acgaatcgaa tgcaccagag cagatcctgt agcccgagac cactgggtcc tggaggagga
     3361 gggattagtg tgagagtgca caggacactg atccagccca cacccggtgc gcccgagaca
     3421 ctttggcctg gagaatctcc cgcccacgca cgccctgctc cgcagccctg ggcgccctct
     3481 ggtggaaagg tagtgactca ggccaggcca tcacctcaat atcctgccca gggatgagct
     3541 gagttctact ttgaccccgg ggagacatgt tccctccccg tgcctcccca ccccctcccc
     3601 ctttaggagt ccattttaat tccagcatgc caatctgagc ctggggcggg ggtggggagc
     3661 agtgtgtgct caagtcatta atcccagaac agaggcagag gcaggtggtg gatctgtgaa
     3721 ctggagggca gcctggtcta cagagtgagt tccaggacag ctagggctac accgagaaac
     3781 cctgtatcga aagataaagg tgtggtgtgg ctagggctgt agttaacagt acttgccctg
     3841 cttacacggg gctcagtttc ccaccttttg catgacctga aaacaaaacc ctttgtagat
     3901 ctctttggat gactaccact ttccaggggc ctggggatgg gggatgggtg tggaggcagt
     3961 gatagttggc tggcacttgg atgctaggca ccaatattct agatttggac ctttaaaact
     4021 gagttcatgc atgcacaggt ctgtggatca cagatgtgat ggggtctgga gattgaaaca
     4081 gggtcctctg gaagagcagc cagtgctttt aacatctctt caggccacat actcagcttt
     4141 taatgataag tttaaccgct gaataatctc tccaggccaa ttactcagcc ttttttttgt
     4201 taagttttgg gaacttaaat tcaggtcttt attgtttgcc cagaaagctc tcttacccac
     4261 tgagccattc cccaaccctt gtttttcatt ttgagacagt ctctctaccc taaactcatt
     4321 ctgctgtcca gggtggccta tgaacttgcc atcctcctgc ctcagtgcct cttgagtagg
     4381 taggtaaggt taaaaatgaa cagcactacc cagctatttt tttttctttt agagtgtgtg
     4441 tgtgtgtgtg tgtgtgtgtg ttttatacag gcaattccat tggcctgctt tcagggaccc
     4501 agccagtgct tacatcatga cctacggcag ccagatgtac cagtagatgt aaggacccac
     4561 ctgccacccc agctctctct ctctgatctc caccaccaat gttccctctc cttctctgtc
     4621 ccctctctct gccctcatga ttctgccccc catctgtctg cctgtctatc tgtccacttg
     4681 tctgcctctg tcccttcacc aagtcccaca gtactcttgc gttcccccca ataaaccttt
     4741 acactaggtc tgttttatgg tgtaacttct caggggtaca ccttggccag gccctccaaa
     4801 aatactccct tgccacatta tatttcataa cggcgtgtaa gcatatatgc caccacagca
     4861 taccttgtga aggtcaagaa caaacagctt gcaggtattg gctttttcca acatatgggt
     4921 tatgaggatt aatctgaggc ttggcagcaa cagcctttct ctactgaggt atcttggcag
     4981 ccccacaccc agcatcttct ttaataaaaa gagttaaaga aaa
//
