LOCUS       NM_001289496            5223 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus EGF-like, fibronectin type III and laminin G domains
            (Egflam), transcript variant 1, mRNA.
ACCESSION   NM_001289496
VERSION     NM_001289496.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Choi,J.S., Ayupe,A.C., Beckedorff,F., Catanuto,P., McCartan,R.,
            Levay,K. and Park,K.K.
  TITLE     Single-nucleus RNA sequencing of developing superior colliculus
            identifies neuronal diversity and candidate mediators of circuit
            assembly
  JOURNAL   Cell Rep 42 (9), 113037 (2023)
   PUBMED   37624694
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Yamamoto,H., Kon,T., Omori,Y. and Furukawa,T.
  TITLE     Functional and Evolutionary Diversification of Otx2 and Crx in
            Vertebrate Retinal Photoreceptor and Bipolar Cell Development
  JOURNAL   Cell Rep 30 (3), 658-671 (2020)
   PUBMED   31968244
REFERENCE   3  (bases 1 to 5223)
  AUTHORS   Orlandi,C., Omori,Y., Wang,Y., Cao,Y., Ueno,A., Roux,M.J.,
            Condomitti,G., de Wit,J., Kanagawa,M., Furukawa,T. and
            Martemyanov,K.A.
  TITLE     Transsynaptic Binding of Orphan Receptor GPR179 to
            Dystroglycan-Pikachurin Complex Is Essential for the Synaptic
            Organization of Photoreceptors
  JOURNAL   Cell Rep 25 (1), 130-145 (2018)
   PUBMED   30282023
REFERENCE   4  (bases 1 to 5223)
  AUTHORS   Kominami,T., Ueno,S., Nakanishi,A., Kominami,A., Kondo,M.,
            Furukawa,T. and Terasaki,H.
  TITLE     Temporal Properties of Cone ERGs of Pikachurin Null Mutant Mouse
  JOURNAL   Invest Ophthalmol Vis Sci 57 (3), 1264-1269 (2016)
   PUBMED   26990161
REFERENCE   5  (bases 1 to 5223)
  AUTHORS   Nagaya,M., Ueno,S., Kominami,T., Nakanishi,A., Koyasu,T., Kondo,M.,
            Furukawa,T. and Terasaki,H.
  TITLE     Pikachurin Protein Required for Increase of Cone Electroretinogram
            B-Wave during Light Adaptation
  JOURNAL   PLoS One 10 (6), e0128921 (2015)
   PUBMED   26091521
  REMARK    GeneRIF: Pikachurin Protein Required for Increase of Cone
            Electroretinogram B-Wave during Light Adaptation
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5223)
  AUTHORS   Omori,Y., Araki,F., Chaya,T., Kajimura,N., Irie,S., Terada,K.,
            Muranishi,Y., Tsujii,T., Ueno,S., Koyasu,T., Tamaki,Y., Kondo,M.,
            Amano,S. and Furukawa,T.
  TITLE     Presynaptic dystroglycan-pikachurin complex regulates the proper
            synaptic connection between retinal photoreceptor and bipolar cells
  JOURNAL   J Neurosci 32 (18), 6126-6137 (2012)
   PUBMED   22553019
REFERENCE   7  (bases 1 to 5223)
  AUTHORS   Hu,H., Li,J., Zhang,Z. and Yu,M.
  TITLE     Pikachurin interaction with dystroglycan is diminished by defective
            O-mannosyl glycosylation in congenital muscular dystrophy models
            and rescued by LARGE overexpression
  JOURNAL   Neurosci Lett 489 (1), 10-15 (2011)
   PUBMED   21129441
  REMARK    GeneRIF: These results indicate that pikachurin interactions with
            alpha-dystroglycan and its localization at the photoreceptor ribbon
            synapse require normal glycosylation of alpha-dystroglycan.
REFERENCE   8  (bases 1 to 5223)
  AUTHORS   Kanagawa,M., Omori,Y., Sato,S., Kobayashi,K., Miyagoe-Suzuki,Y.,
            Takeda,S., Endo,T., Furukawa,T. and Toda,T.
  TITLE     Post-translational maturation of dystroglycan is necessary for
            pikachurin binding and ribbon synaptic localization
  JOURNAL   J Biol Chem 285 (41), 31208-31216 (2010)
   PUBMED   20682766
  REMARK    GeneRIF: Post-translational maturation of dystroglycan is necessary
            for pikachurin binding and ribbon synaptic localization.
REFERENCE   9  (bases 1 to 5223)
  AUTHORS   Manabe,R., Tsutsui,K., Yamada,T., Kimura,M., Nakano,I., Shimono,C.,
            Sanzen,N., Furutani,Y., Fukuda,T., Oguri,Y., Shimamoto,K.,
            Kiyozumi,D., Sato,Y., Sado,Y., Senoo,H., Yamashina,S., Fukuda,S.,
            Kawai,J., Sugiura,N., Kimata,K., Hayashizaki,Y. and Sekiguchi,K.
  TITLE     Transcriptome-based systematic identification of extracellular
            matrix proteins
  JOURNAL   Proc Natl Acad Sci U S A 105 (35), 12849-12854 (2008)
   PUBMED   18757743
REFERENCE   10 (bases 1 to 5223)
  AUTHORS   Sato,S., Omori,Y., Katoh,K., Kondo,M., Kanagawa,M., Miyata,K.,
            Funabiki,K., Koyasu,T., Kajimura,N., Miyoshi,T., Sawai,H.,
            Kobayashi,K., Tani,A., Toda,T., Usukura,J., Tano,Y., Fujikado,T.
            and Furukawa,T.
  TITLE     Pikachurin, a dystroglycan ligand, is essential for photoreceptor
            ribbon synapse formation
  JOURNAL   Nat Neurosci 11 (8), 923-931 (2008)
   PUBMED   18641643
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC109195.27 and AC158747.3.
            
            On Feb 3, 2023 this sequence version replaced NM_001289496.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC095994.1, DQ223720.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-735               AC109195.27        118641-119375
            736-845             AC158747.3         11727-11836
            846-929             AC158747.3         13595-13678
            930-1047            AC158747.3         24858-24975
            1048-1183           AC158747.3         26017-26152
            1184-1350           AC158747.3         40127-40293
            1351-1466           AC158747.3         75533-75648
            1467-1785           AC158747.3         76331-76649
            1786-1886           AC158747.3         77571-77671
            1887-1987           AC158747.3         78783-78883
            1988-2132           AC158747.3         79903-80047
            2133-2322           AC158747.3         82888-83077
            2323-2448           AC158747.3         86169-86294
            2449-2692           AC158747.3         87432-87675
            2693-2804           AC158747.3         92229-92340
            2805-2921           AC158747.3         95776-95892
            2922-3102           AC158747.3         98309-98489
            3103-3126           AC158747.3         105409-105432
            3127-3205           AC158747.3         107251-107329
            3206-3349           AC158747.3         110309-110452
            3350-3433           AC158747.3         117863-117946
            3434-3537           AC158747.3         121472-121575
            3538-5223           AC158747.3         122275-123960
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 3.5 cM"
     gene            1..5223
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="EGF-like, fibronectin type III and laminin G
                     domains"
                     /db_xref="GeneID:268780"
                     /db_xref="MGI:MGI:2146149"
     exon            1..735
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     CDS             639..3692
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; pikachurin; nectican; EGF-like, fibronectin
                     type-III and laminin G-like domain-containing protein"
                     /codon_start=1
                     /product="pikachurin isoform 1 precursor"
                     /protein_id="NP_001276425.1"
                     /db_xref="CCDS:CCDS79359.1"
                     /db_xref="GeneID:268780"
                     /db_xref="MGI:MGI:2146149"
                     /translation="MDLISTFSLHFLLLACSLPPGAVSLRTALRKSGKVGPPLDIKLG
                     ALNCTAFSIQWKTPKRSGSSIIGYTVFYSEVGSDKSLRERSHNVPVGQDTLITEEVIG
                     DLKPGTEYQVSVAAYSQTGKGRLSFPRHVTTLSQDSCLPPAAPQQPHVLVVSDSEVAL
                     SWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDTNYQFAVKA
                     MNAHGFSPRSWPSNTVRTLGPGEAGSGHYGPGYITNPGVSEDDDGSEDELDLDVSFEE
                     VKPLPATKVGNKKFSVESKKTSVSNSVMGSRLAQPTSASLHETTVAIPPTPAQRKGKN
                     SVAMMSRLFDMSCDETLCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFF
                     GHSYVTFEPLKNSYQAFQVTLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFR
                     FNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRT
                     PLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGADVGECSS
                     GICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPW
                     PLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTGSKDFLSINMAAGHVEFRFDCGSGTG
                     VLRSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVP
                     NYDDVKKNSGILHPFSGSIQKIILNDRTIHVKHDFTSGVNVENAAHPCVGAPCAHGGS
                     CRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQFIGRSYLTYDNPNILKR
                     VSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSGVASI
                     MVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEI
                     ALHTNRQYLRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK"
     exon            736..845
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            846..929
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1047
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1183
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1350
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1466
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1785
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1886
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..1987
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2132
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2322
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2448
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..2692
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2804
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2921
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            2922..3102
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            3103..3126
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3205
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            3206..3349
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..3433
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            3434..3537
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     exon            3538..5223
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4272..4277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="hexamer: AATAAA"
     polyA_site      4302
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
     regulatory      4671..4676
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="hexamer: ATTAAA"
     polyA_site      4692
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="major polyA site"
     regulatory      4736..4741
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="hexamer: AATAAA"
     regulatory      4746..4751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="hexamer: AATAAG"
     polyA_site      4756
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
     polyA_site      4762
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
     regulatory      5198..5203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
                     /note="hexamer: AATAAA"
     polyA_site      5223
                     /gene="Egflam"
                     /gene_synonym="5930412K08"
ORIGIN      
        1 gcttcttcca agagtgctct acagagtaga gaaacctttg aggtattagg aaaagtggtt
       61 taaactggga gcaagccacc tagaggggta tgggggaaga aaggtccagc cagcgaaacc
      121 gaagcaccaa accaaaagtt aagggttagg atccagccga ggaccctggg cccgcagtct
      181 gagtcaagga acaccgggcg agtgtcgaag tcggtcccac cccggccccc aatctaggtc
      241 acccgcgggg aagggctgag ctgggggctg tcggcgccgg aggtgggcgc ggagtgaatt
      301 gattcagacc ctgcccctcg ttcttccact agattgtaat tccatctcct gcgggtcgag
      361 ccgccctccc tccggcggcc agtgcgtccc tcccctcggc tctcagcgtc cactccccag
      421 ccccgaaaag ccttgccgct gcgagcccgc tccgctcaag cacgccagct tcccaactgc
      481 tgccttgcct cctcggactc ccgagtggct cctggttcag ctccctgggt ctcccacctt
      541 ctcatcctcg cgtcactggc gttccccgcg tcccccagga gacgcccttt ccctgcgcgc
      601 ccgggacttg gggaaacttt gcagacgccg ttggtgagat ggatttaatc tcaaccttct
      661 cgctccattt cttgctcctg gcctgcagcc tcccacccgg cgctgtgtca ctccgaacgg
      721 ctctgcgaaa atcaggcaag gtgggacctc ctcttgacat caagctggga gccttgaact
      781 gtacagcttt cagcatccag tggaaaacac caaagcgttc tggtagctcc atcattggct
      841 acacagtgtt ttactctgag gttggttcag ataagtccct gcgggagcgg tcacacaacg
      901 tcccggttgg ccaggatacc ctaatcactg aggaagtgat tggagatttg aagccaggca
      961 ctgaatatca agtaagcgta gcagcctaca gtcagactgg caaggggagg cttagcttcc
     1021 ctcgacatgt gaccactttg tctcaagatt cctgcctgcc ccctgcagca ccccaacagc
     1081 cacatgtcct tgtggtttcc gattctgaag tggccctttc ttggagacct ggagagaatg
     1141 aaggaagtgc cccaattcag tcctactctg tggaattcat tagaccagat tttgacaaga
     1201 gctggaccat aatccaagag cgcctgcaga tggactccat ggttatcaaa ggccttgatc
     1261 cagataccaa ctaccagttt gctgtgaaag ccatgaatgc tcacggcttc agtccgcgca
     1321 gctggcccag caacaccgtc cgaaccctgg gtcccgggga ggctggaagt ggtcattatg
     1381 gacctgggta tatcaccaat cccggtgtga gtgaagatga tgatggatct gaagacgaac
     1441 tggacctgga tgtatccttt gaggaggtta aaccacttcc tgctaccaaa gttgggaata
     1501 agaagttctc agtggagtcc aagaagactt ctgtctccaa ctcagtgatg ggctccagac
     1561 tcgcccaacc tacctcagcc tctctccacg aaactacagt ggccatccca cccacaccag
     1621 cccaacggaa ggggaagaac agtgtggcca tgatgtcaag gctctttgac atgtcctgtg
     1681 atgaaactct ctgttcagct gacagcttct gtgtcaatga ctacgcctgg gggggctcac
     1741 gatgccattg caacctgggc aaaggaggcg aggcctgctc agaagatatc tttattcagt
     1801 atcctcagtt ctttggccac tcctacgtaa cctttgagcc tctgaagaac tcttatcagg
     1861 catttcaggt tactctggag ttcagggcag aggcggagga tggcttgctg ctctactgcg
     1921 gagagagtga gcacggaaga ggagacttca tgtccctggc tctcatccgg cggtccctcc
     1981 acttcaggtt taattgtgga actgggattg ccatcatcat aagtgagacc aaaatcaaac
     2041 ttggggcctg gcatacggtg acactgtaca gagatggcct gaatgggatg ctgcagttga
     2101 ataatggcac cccagtgaca ggccagtccc agggtcagta cagtaaaatt accttccgga
     2161 cacctctgta cctcgggggc gctcccagtg cttactggct ggtcagagca acagggacaa
     2221 accgtggctt ccaaggctgt gtgcagtcac tttctgttaa tgggaagaag attgacatga
     2281 ggccctggcc attaggaaaa gccctcaatg gagctgatgt tggggagtgc agcagtggga
     2341 tctgtgacga agcctcctgc atccacgggg gcacctgcgc agccatcaag gccgactcct
     2401 acatctgcct ctgtcccctt gggttcagag gtcgacactg tgaagatgct tttgccctga
     2461 ccatccctca gttcagagag tcgctgaggt cctatgctgc cacaccctgg cccctggagc
     2521 ctcagcatta tctttctttc accgagttcg agatcacctt tcggccagac tcgggggatg
     2581 gtgtcctttt gtacagctac gacacgggca gcaaggactt cctctccatc aatatggctg
     2641 cgggccacgt ggaattccgc ttcgactgtg gttctgggac gggagttctc aggagtgagg
     2701 ctcccctcac cctaggccaa tggcatgacc tacgtgtgtc tcgcacagcg aagaatggga
     2761 tcttacaggt ggacaagcag aaggtggtag agggaatggc agagggaggc ttcacccaga
     2821 ttaagtgtaa tacggacatt tttattggtg gagtcccaaa ttacgatgat gtcaagaaga
     2881 actcggggat cctccatcca tttagtggga gcattcagaa gatcatcctc aacgaccgga
     2941 ccatccatgt gaagcatgac tttacatcgg gcgtgaatgt ggagaacgca gcccacccct
     3001 gtgtaggggc cccctgtgcc catgggggca gctgtcggcc caggaaggag ggttatgagt
     3061 gcgactgtcc cttgggcttc gaaggcctga actgccagaa agagtgtggg aaccactgcc
     3121 tcaatactat tatagaagcc atcgagatcc cacagttcat cggccgcagc tacctgacgt
     3181 atgacaaccc aaatatcctc aagagggtgt ctggatcgag gtcaaatgcg ttcatgaggt
     3241 ttaagacaac cgccaaggat ggcctgttgc tgtggagggg ggacagcccc atgagaccta
     3301 acagtgactt catttccctg ggtcttcggg atggagccct gatattcagc tacaacctgg
     3361 gcagtggcgt ggcttccatc atggtgaacg gttccttcag cgatggccgg tggcaccgag
     3421 tcaaggctgt cagggatggc cagtccggga agattacagt ggatgactac ggagccagaa
     3481 caggcaagtc accgggcttg atgcggcagc ttaacatcaa cggtgctctg tacgtgggtg
     3541 gaatgaagga aatcgctctg cacacgaaca gacaatacct gcgaggcctt gtgggctgca
     3601 tctcccattt cacactgtcc accgattacc acatttccct cgtggaagac gccgtggatg
     3661 ggaagaacat caacacttgt ggggctaagt aacaccagct gaccttgccc aagggacata
     3721 gcctctatcc tgcaatcaca gaggcccgga gaccctgcct gatgctgcac tcagaggtct
     3781 tgaaatcagg tgtgtttact ttctctgagg aggacaaagt acaccctggt gagagccaag
     3841 actgaaggcc ctgggtggcc ttctctgcta acgccctgtg aaccgaaccc aatggactac
     3901 tgtggttagg aagcattgga ctttgttgtt caacgtatag agtctgagag agatgatacg
     3961 tcagtgcaag ctctgggaac tctctgctgt agctcaatgg ctctgttgtg ttaacaagag
     4021 tgagagccag actggatgag aaagagtgaa agagcaagag agagagagag acaagacaga
     4081 gagacagaga gacagagaga ccacccctcc aaatagtatt aaaatatttc tgctcttccc
     4141 tgacttatga cactagcagg aagactgggc aaccctgaac ttcatacttc caatctcggt
     4201 ctatggattg tgtagattaa ccccttccat gccctgctgg ctgactcaat gtgctctgac
     4261 tagtccatgg aaataaatgt tgggggtaaa ttaaacatag aataataatc agactgtggc
     4321 atccttcccc caagctttcc tctaatgcaa atattctctc tttttttaat gatgcagatc
     4381 gaagaaagcg ggccccctga ttttgtagct gtactttttg tatgtgacta ttttttatag
     4441 ctgcagtctg tccacaccca tggtgtgctt tttgaaagtt agtggttgat cctaattacg
     4501 aaacaccttc ctaatagctt tctcacaatg gagatgtgat caggccattt gtagtgtcag
     4561 gggagcggta gcaagtgact agggtgggcc attgggagaa gggaccattc tcttttagta
     4621 tatgctgacg gttcaagtgg atgaatatga tgctgtccaa aagtggagag attaaaagga
     4681 atgtatcacc gaggtgggtc attaccgttg ttattttaag agagggaaag tgtgtaataa
     4741 atatgaataa gggaaagagg aagaactcat tcagtcattt gtgaaggtgt gactgatcct
     4801 gcagaggcca catctgcaaa gggacaacaa ggttactaga gagtcggatg ttgtgtggga
     4861 cctagggcca tgtcctcagc agggtctgag gatctgggtc cgacagagaa gggtgctttc
     4921 ttcttctgta ccatggttca gctgtgccct gtgctatgca tacacatgac tgcagacagc
     4981 tggctccctg gctggtcccc acaaggactt actaagggaa aggtgagatg aatgttgccc
     5041 ctctaagtgg aaggaaatac gatccagctc ctggaagagc ctataattag tctcaggcac
     5101 gactctattc ttgtttgtgc agcatccatt cagcagagac ttgagctttg tgcagagcac
     5161 agtttttagg catattaaga cgtctaagag atggctaaat aaaccagaga ggtttgcccc
     5221 aca
//
