LOCUS       NM_001403845            1335 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus EGF-like domain 8 (Egfl8), transcript variant 1, mRNA.
ACCESSION   NM_001403845 XM_006525179
VERSION     NM_001403845.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Chen,J., Zhang,X., DeLaughter,D.M., Trembley,M.A., Saifee,S.,
            Xiao,F., Chen,J., Zhou,P., Seidman,C.E., Seidman,J.G. and Pu,W.T.
  TITLE     Molecular and Spatial Signatures of Mouse Embryonic Endothelial
            Cells at Single-Cell Resolution
  JOURNAL   Circ Res 134 (5), 529-546 (2024)
   PUBMED   38348657
REFERENCE   3  (bases 1 to 1335)
  AUTHORS   Lim,Y.S., Lee,D.Y., Kim,H.Y., Ok,Y., Hwang,S., Moon,Y. and Yoon,S.
  TITLE     Descriptive and functional characterization of epidermal growth
            factor-like domain 8 in mouse cortical thymic epithelial cells by
            integrated analysis of gene expression signatures and networks
  JOURNAL   Int J Mol Med 47 (3) (2021)
   PUBMED   33448309
  REMARK    GeneRIF: Descriptive and functional characterization of epidermal
            growth factorlike domain 8 in mouse cortical thymic epithelial
            cells by integrated analysis of gene expression signatures and
            networks.
REFERENCE   4  (bases 1 to 1335)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 1335)
  AUTHORS   Song,I.J., Ikram,M., Subhan,F., Choi,D.J., Lee,J.R., Kim,H.S.,
            Lim,Y.T. and Yoon,S.
  TITLE     Molecular characterization and expression analysis of mouse
            epidermal growth factor-like domain 8
  JOURNAL   Int J Mol Med 36 (2), 541-550 (2015)
   PUBMED   26096343
  REMARK    GeneRIF: Mouse EGFL8 encodes a protein of 293 amino acids with two
            EGFL domains, an Emilin-like domain and a Ca(2+)-binding EGFL
            domain, which has a molecular mass of 32 kDa.
REFERENCE   6  (bases 1 to 1335)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   7  (bases 1 to 1335)
  AUTHORS   Fitch,M.J., Campagnolo,L., Kuhnert,F. and Stuhlmann,H.
  TITLE     Egfl7, a novel epidermal growth factor-domain gene expressed in
            endothelial cells
  JOURNAL   Dev Dyn 230 (2), 316-324 (2004)
   PUBMED   15162510
REFERENCE   8  (bases 1 to 1335)
  AUTHORS   Xie,T., Rowen,L., Aguado,B., Ahearn,M.E., Madan,A., Qin,S.,
            Campbell,R.D. and Hood,L.
  TITLE     Analysis of the gene-dense major histocompatibility complex class
            III region and its comparison to mouse
  JOURNAL   Genome Res 13 (12), 2621-2636 (2003)
   PUBMED   14656967
REFERENCE   9  (bases 1 to 1335)
  AUTHORS   Allcock,R.J., Martin,A.M. and Price,P.
  TITLE     The mouse as a model for the effects of MHC genes on human disease
  JOURNAL   Immunol Today 21 (7), 328-332 (2000)
   PUBMED   10871872
  REMARK    Review article
REFERENCE   10 (bases 1 to 1335)
  AUTHORS   Yung Yu,C., Yang,Z., Blanchong,C.A. and Miller,W.
  TITLE     The human and mouse MHC class III region: a parade of 21 genes at
            the centromeric segment
  JOURNAL   Immunol Today 21 (7), 320-328 (2000)
   PUBMED   10871871
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT009767.6.
            
            On Apr 5, 2022 this sequence version replaced XM_006525179.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784650.742903.1,
                                           SRR17784643.1501142.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-245               CT009767.6         173156-173400       c
            246-365             CT009767.6         172594-172713       c
            366-488             CT009767.6         172306-172428       c
            489-598             CT009767.6         172096-172205       c
            599-694             CT009767.6         171911-172006       c
            695-865             CT009767.6         171597-171767       c
            866-948             CT009767.6         171436-171518       c
            949-1102            CT009767.6         171204-171357       c
            1103-1335           CT009767.6         170776-171008       c
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 18.19 cM"
     gene            1..1335
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /note="EGF-like domain 8"
                     /db_xref="GeneID:81701"
                     /db_xref="MGI:MGI:1932094"
     exon            1..245
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            246..365
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..1146
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /note="epidermal growth factor-like protein 8; EGF-like
                     protein 8"
                     /codon_start=1
                     /product="epidermal growth factor-like protein 8
                     precursor"
                     /protein_id="NP_001390774.1"
                     /db_xref="GeneID:81701"
                     /db_xref="MGI:MGI:1932094"
                     /translation="MGLWAELCISLRGLSFFLVLMTGEGTRGGSFKESLGVCSKQTLL
                     VPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKK
                     PHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCL
                     NTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAE
                     LRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEER
                     LGACACEDNSLGPSLRG"
     exon            366..488
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            489..598
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            599..694
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            695..865
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            866..948
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1102
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1335
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1309..1314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /note="hexamer: AATAAA"
     polyA_site      1335
                     /gene="Egfl8"
                     /gene_synonym="Ng3"
                     /note="major polyA site"
ORIGIN      
        1 ccataattca gggacctccg aaattgtgta ggtcctgctg gagcaacact tggctgaaga
       61 gaagaaggtg aggaaggcag ggcctgtcta ctgagccaac cagcctgggt ctgaggagct
      121 agtgggaggg agactgctgt gtggtccagc ccctctgcat cttctcagcc agagaggccc
      181 acccaccagt gtaagctgcg gctgctgggg ctgttgctgc tgcagcctcc tggacaggga
      241 aacagccctc agagtgaggg agccatgggg ctctgggctg agctgtgcat ttccctacga
      301 ggactctcat tcttcctggt gctgatgacg ggcgaaggga ccagaggagg atctttcaaa
      361 gagagtttgg gagtgtgctc caagcagacg ctgctggttc ctctccgtta caacgagtcc
      421 tatagtcaac cggtgtacaa accctacctg accttgtgtg cggggaggcg catatgtagc
      481 acctacagga ccacataccg tgtggcctgg cgggaggtga ggcgggaggt accacagaca
      541 cacgtggtgt gctgtcaggg ctggaagaag ccacacccag gagctctcac ctgtgatgcc
      601 atctgctcca agccttgtct taatggaggt gtctgcactg gaccagaccg gtgcgagtgt
      661 gccccaggct ggggaggaaa gcattgccac gtggatgtcg atgaatgcag ggccagcctt
      721 accctctgct ctcatggctg cctcaacaca ctgggcagct tcttgtgcag ctgtccacac
      781 cccctggtgc tgggtctcga tggacgcacc tgtgcagggg ggcctccaga gtccccgaca
      841 agtgccagca tcctcagtgt ggcagttcgg gaagcagaca gcgaagagga gcgagccctg
      901 aggtgggagg ttgcagagct tcgtgggcgt ctggagaagc tggagcagtg ggccacacag
      961 gctggggcct gggttcgagc agtgctgccc atgccccctg aagagctgcg gcctgaacag
     1021 gtggctgagc tttggggccg gggtgacaga attgagtccc tcagcgacca ggtgctgcta
     1081 ctggaggaaa gactgggtgc ctgtgcctgt gaggataaca gcctgggccc aagcctgcgt
     1141 ggatgacgag gaatgcccgc agcagctggg gtccccaact tacacagaag ctacacctgc
     1201 taacctggga tcagaagata tggatatcaa ccactgaagt acaacaggcc gcagagaacc
     1261 ccacgtctct ctttctcttt attttcagtt gcttgctgtt atccagataa taaaaaccaa
     1321 ccacacaaaa ctgta
//
