LOCUS       NM_010113               4773 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus epidermal growth factor (Egf), transcript variant 1,
            mRNA.
ACCESSION   NM_010113
VERSION     NM_010113.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Zheng,Q., Li,M., Chen,L., Zhang,C., Zhao,Y., Liu,G., Yang,F. and
            Zhan,J.
  TITLE     Potential therapeutic target of EGF on bile duct ligation model and
            biliary atresia children
  JOURNAL   Pediatr Res 94 (4), 1297-1307 (2023)
   PUBMED   37138025
  REMARK    GeneRIF: Potential therapeutic target of EGF on bile duct ligation
            model and biliary atresia children.
REFERENCE   3  (bases 1 to 4773)
  AUTHORS   Zhang,X., Xiao,G., Johnson,C., Cai,Y., Horowitz,Z.K., Mennicke,C.,
            Coffey,R., Haider,M., Threadgill,D., Eliscu,R., Oldham,M.C.,
            Greenbaum,A. and Ghashghaei,H.T.
  TITLE     Bulk and mosaic deletions of Egfr reveal regionally defined
            gliogenesis in the developing mouse forebrain
  JOURNAL   iScience 26 (3), 106242 (2023)
   PUBMED   36915679
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4773)
  AUTHORS   Guo,C., Kong,X., Fan,Y. and Zhang,R.
  TITLE     Aerobic Treadmill Exercise Upregulates Epidermal Growth Factor
            Levels and Improves Learning and Memory in d-galactose-Induced
            Aging in a Mouse Model
  JOURNAL   Am J Alzheimers Dis Other Demen 38, 15333175231211082 (2023)
   PUBMED   37977137
  REMARK    GeneRIF: Aerobic Treadmill Exercise Upregulates Epidermal Growth
            Factor Levels and Improves Learning and Memory in
            d-galactose-Induced Aging in a Mouse Model.
REFERENCE   5  (bases 1 to 4773)
  AUTHORS   Lamontagne,J.O., Zhang,H., Zeid,A.M., Strittmatter,K., Rocha,A.D.,
            Williams,T., Zhang,S. and Marneros,A.G.
  TITLE     Transcription factors AP-2alpha and AP-2beta regulate distinct
            segments of the distal nephron in the mammalian kidney
  JOURNAL   Nat Commun 13 (1), 2226 (2022)
   PUBMED   35468900
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4773)
  AUTHORS   Kohda,D. and Inagaki,F.
  TITLE     Three-dimensional nuclear magnetic resonance structures of mouse
            epidermal growth factor in acidic and physiological pH solutions
  JOURNAL   Biochemistry 31 (47), 11928-11939 (1992)
   PUBMED   1445923
REFERENCE   7  (bases 1 to 4773)
  AUTHORS   Dardik,A., Smith,R.M. and Schultz,R.M.
  TITLE     Colocalization of transforming growth factor-alpha and a functional
            epidermal growth factor receptor (EGFR) to the inner cell mass and
            preferential localization of the EGFR on the basolateral surface of
            the trophectoderm in the mouse blastocyst
  JOURNAL   Dev Biol 154 (2), 396-409 (1992)
   PUBMED   1426645
REFERENCE   8  (bases 1 to 4773)
  AUTHORS   Yeh,J., Rosen,K.M. and Villa-Komaroff,L.
  TITLE     Messenger ribonucleic acid for transforming growth factor-alpha,
            but not for epidermal growth factor, is expressed in fetal and
            neonatal mouse brain
  JOURNAL   Am J Obstet Gynecol 167 (1), 242-245 (1992)
   PUBMED   1442934
REFERENCE   9  (bases 1 to 4773)
  AUTHORS   Hiramatsu,M., Kashimata,M., Takayama,F., Tsubakida,K., Ri,K. and
            Minami,N.
  TITLE     Reduced level of epidermal growth factor in the skeletal muscle of
            mice with muscular dystrophy
  JOURNAL   Horm Metab Res 24 (3), 138-139 (1992)
   PUBMED   1577403
REFERENCE   10 (bases 1 to 4773)
  AUTHORS   Ramesh,V., Cheng,S.V., Kozak,C.A., Herron,B.J., Shih,V.E.,
            Taylor,B.A. and Gusella,J.F.
  TITLE     Mapping of ornithine aminotransferase gene sequences to mouse
            chromosomes 7, X, and 3
  JOURNAL   Mamm Genome 3 (1), 17-22 (1992)
   PUBMED   1349842
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC098732.3, AK136439.1 and
            BP761200.1.
            
            On Jul 18, 2015 this sequence version replaced NM_010113.3.
            
            Summary: This gene encodes epidermal growth factor (EGF), the
            founding member of the EGF family of growth factors that are
            implicated in cell proliferation and differentiation. The encoded
            protein can localize to the membrane and function in juxtacrine
            signaling or undergo proteolytic processing to generate a soluble
            form of the hormone. Mice lacking the encoded protein do not
            exhibit an abnormal phenotype but transgenic mice overexpressing
            the encoded protein exhibit hypospermatogenesis. [provided by
            RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060741.1, V00741.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-506               AC098732.3         264133-264638       c
            507-706             AC098732.3         249158-249357       c
            707-888             AC098732.3         246711-246892       c
            889-1113            AC098732.3         246073-246297       c
            1114-1316           AC098732.3         245118-245320       c
            1317-1442           AC098732.3         234431-234556       c
            1443-2294           AK136439.1         36-887
            2295-2295           AC098732.3         220703-220703       c
            2296-4754           AK136439.1         889-3347
            4755-4773           BP761200.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 58.5 cM"
     gene            1..4773
                     /gene="Egf"
                     /note="epidermal growth factor"
                     /db_xref="GeneID:13645"
                     /db_xref="MGI:MGI:95290"
     exon            1..506
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     CDS             359..4012
                     /gene="Egf"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; Pro-epidermal growth factor precursor (EGF)"
                     /codon_start=1
                     /product="pro-epidermal growth factor isoform 1
                     preproprotein"
                     /protein_id="NP_034243.2"
                     /db_xref="CCDS:CCDS17833.1"
                     /db_xref="GeneID:13645"
                     /db_xref="MGI:MGI:95290"
                     /translation="MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSER
                     SGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVER
                     QVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVL
                     LSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDK
                     RLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIW
                     IANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFG
                     LCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGF
                     VLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDN
                     GGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTD
                     YKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGL
                     ALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVG
                     MSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKR
                     RRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVV
                     HPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGEN
                     ADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSD
                     GETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDG
                     ISCFDIDECQRGAHNCGENAACTNTEGGYNCTCAGRPSSPGLSCPDSTAPSLLGEDGH
                     HLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRH
                     AGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSG
                     PNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTS
                     WRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHE
                     RAPDLPRQTEPVQ"
     exon            507..706
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            707..888
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1113
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1316
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1442
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1565
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1688
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1814
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..1951
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2100
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2205
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..2429
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            2430..2597
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2744
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            2745..2870
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            2871..2987
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            2988..3113
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            3114..3236
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            3237..3381
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            3382..3549
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            3550..3670
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            3671..3776
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..4755
                     /gene="Egf"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4733..4738
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Egf"
                     /note="hexamer: AATAAA"
     polyA_site      4755
                     /gene="Egf"
                     /note="major polyA site"
ORIGIN      
        1 ggttccaaaa ggagaaggga ttcctatctg tatataggga aggaatccta tctgcatatt
       61 tcgttgttag caccatccct catcccggtg gggcttggaa ctttccatca attctttcct
      121 gtctcgtttc tctttcatcc tttgcctggt tgtgcctgtc tcagggagaa atcagtcacc
      181 tgcaggcctt gcagggctct taggctctgg gaaatttgtc atacgggtgt caggtacttc
      241 ttattgctgt ccaaagggaa aaaaaaagtg agacaaagaa ctctcccgga gcctttccgg
      301 ctgcactcag aggctctcga gaggtgcaga aggacctgga aaggcagcta aataaaagat
      361 gccctggggc cgaaggccaa cctggctgtt gctcgccttc ctgctggtgt ttttaaagat
      421 tagcatactc agcgtcacag catggcagac cgggaactgt cagccaggtc ctctcgagag
      481 aagcgagaga agcgggactt gtgccggtcc tgcccccttc ctagttttct cacaaggaaa
      541 gagcatctct cggattgacc cagatggaac aaatcaccag caattggtgg tggatgctgg
      601 catctcagca gacatggata ttcattataa aaaagagaga ctctattggg tggatgtaga
      661 aagacaagtt ttgctaagag ttttccttaa cgggacagga ctagagaaag tgtgcaatgt
      721 agagaggaag gtgtctgggc tggccataga ctggatagat gatgaagttc tctgggtaga
      781 ccaacagaac ggagtcatca ccgtaacaga tatgacaggg aaaaattccc gagttcttct
      841 aagttcctta aaacatccgt caaatatagc agtggatcca atagagaggt tgatgttttg
      901 gtcttcagag gtgaccggca gccttcacag agcacacctc aaaggtgttg atgtaaaaac
      961 actgctggag acagggggaa tatcggtgct gactctggat gtcctggaca aacggctctt
     1021 ctgggttcag gacagtggcg aaggaagcca cgcttacatt cattcctgtg attatgaggg
     1081 tggctccgtc cgtcttatca ggcatcaagc acggcacagt ttgtcttcaa tggccttttt
     1141 tggtgatcgg atcttctact cagtgttgaa aagcaaggcg atttggatag ccaacaaaca
     1201 cacggggaag gacacggtca ggattaacct ccatccatcc tttgtgacac ctggaaaact
     1261 gatggtagta caccctcgtg cacagcccag gacagaggac gctgctaagg atcctgaccc
     1321 cgaacttctc aaacagaggg gaagaccatg ccgcttcggt ctctgtgagc gagaccccaa
     1381 gtcccactcg agcgcatgcg ctgagggcta cacgttaagc cgagaccgga agtactgcga
     1441 agatgtcaat gaatgtgcca ctcagaatca cggctgtact cttgggtgtg aaaacacccc
     1501 tggatcctat cactgcacat gccccacagg atttgttctg cttcctgatg ggaaacaatg
     1561 tcacgaactt gtttcctgcc caggcaacgt atcaaagtgc agtcatggct gtgtcctgac
     1621 atcagatggt ccccggtgca tctgtcctgc aggttcagtg cttgggagag atgggaagac
     1681 ttgcactggt tgttcatcgc ctgacaatgg tggatgcagc cagatctgtc ttcctctcag
     1741 gccaggatcc tgggaatgtg attgctttcc tgggtatgac ctacagtcag accgaaagag
     1801 ctgtgcagct tcaggaccac agccactttt actgtttgca aattcccagg acatccgaca
     1861 catgcatttt gatggaacag actacaaagt tctgctcagc cggcagatgg gaatggtttt
     1921 tgccttggat tatgaccctg tggaaagcaa gatatatttt gcacagacag ccctgaagtg
     1981 gatagagagg gctaatatgg atgggtccca gcgagaaaga ctgatcacag aaggagtaga
     2041 tacgcttgaa ggtcttgccc tggactggat tggccggaga atctactgga cagacagtgg
     2101 gaagtctgtt gttggaggga gcgatctgag cgggaagcat catcgaataa tcatccagga
     2161 gagaatctcg aggccgcgag gaatagctgt gcatccaagg gccaggagac tgttctggac
     2221 ggacgtaggg atgtctccac ggattgaaag cgcttccctt caaggttccg accgggtgct
     2281 gatagccagc tccaatctac tggaacccag tggaatcacg attgactact taacagacac
     2341 tttgtactgg tgtgacacca agaggtctgt gattgaaatg gccaatctgg atggctccaa
     2401 acgccgaaga cttatccaga acgacgtagg tcaccccttc tctctagccg tgtttgagga
     2461 tcacctgtgg gtctcggatt gggctatccc atcggtaata agggtgaaca agaggactgg
     2521 ccaaaacagg gtacgtcttc aaggcagcat gctgaagccc tcgtcactgg ttgtggtcca
     2581 tccattggca aaaccaggtg cagatccctg cttatacagg aatggaggct gtgaacacat
     2641 ctgccaagag agcctgggca cagctcggtg tttgtgtcgt gaaggttttg tgaaggcctg
     2701 ggatgggaaa atgtgtctcc ctcaggatta tccaatcctg tcaggtgaaa atgctgatct
     2761 tagtaaagag gtgacatcac tgagcaactc cactcaggct gaagtaccag acgatgatgg
     2821 gacagaatct tccacactag tggctgaaat catggtgtca ggcatgaact atgaagatga
     2881 ctgtggtccc ggggggtgtg gaagccatgc tcgatgcgtt tcagacggag agactgctga
     2941 gtgtcagtgt ctgaaagggt ttgccaggga tggaaacctg tgttctgata tagatgagtg
     3001 tgtgctggct agatcggact gccccagcac ctcgtccagg tgcatcaaca ctgaaggtgg
     3061 ctacgtctgc agatgctcag aaggctacga aggagacggg atctcctgtt tcgatattga
     3121 cgagtgccag cggggggcgc acaactgcgg tgagaatgcc gcctgcacca acacggaggg
     3181 aggctacaac tgcacctgcg caggccgccc atcctcgccc ggactgagtt gccctgactc
     3241 taccgcaccc tctctccttg gggaagatgg ccaccatttg gaccgaaata gttatccagg
     3301 atgcccatcc tcatatgatg gatactgcct caatggtggc gtgtgcatgc atattgaatc
     3361 actggacagc tacacatgca actgtgttat tggctattct ggggatcgat gccagactcg
     3421 agacctacga tggtgggaac tgcgtcatgc tggctacggg cagaagcatg acatcatggt
     3481 ggtggctgtc tgcatggtgg cactggtcct gctgctcgtc ttggggatgt gggggactta
     3541 ctactacagg actcggaagc agctatcaaa ccccccaaag aacccttgtg atgagccaag
     3601 cggaagtgtg agcagcagcg gacccaacag cagcagcggg gcagctgtgg cttcttgtcc
     3661 ccaaccttgg tttgtggtcc tagagaaaca ccaagacccc aagaatggga gtctgcctgc
     3721 ggatggtacg aatggtgcag tagtagatgc tggcctgtct ccctccctgc agctcgggtc
     3781 agtgcatctg acttcatgga gacagaagcc ccacatagat ggaatgggca cagggcaaag
     3841 ctgctggatt ccaccatcaa gtgacagagg accccaggaa atagagggaa actcccacct
     3901 accctcctac agacctgtgg ggccggagaa gctgcattct ctccagtcag ctaatggatc
     3961 gtgtcacgaa agggctccag acctgccacg gcagacagag ccagttcagt agaaactggg
     4021 agtagacaga aggtacagaa gggaaaataa caaaccaggc tgatgatggt agaatgctac
     4081 agacttggta ctccagtttc cacggctaat cactgctcgc tcagggtcct gaagatagct
     4141 gcacagctgc agagctgcac agcgggatag ctgcgacttt tgcttcttgc tttaagcagt
     4201 tccactgaag atactcaaaa gagaagtgga gaaaatcatt agaaaccaaa gtcaagacat
     4261 tcatatataa gctgtgtctt cttcactgga cggtttgcct cttttccttt tgcctcagaa
     4321 ggagtgggtt aaagcaggtg accccatgct ctgtcaaccc ctgaataaat gatgtgatct
     4381 acatagaagt cttagctcac tctcaggaac gcttggaaca ctataacttt tgctatgata
     4441 tactgccaag tgtggcccat gctcataatt gtgctttctg aattgtgata aattagtgaa
     4501 aaaactgtaa cttagaatct gatttattca ggattagatc atctttttat actataaaaa
     4561 tcttcgaatg aaaatattta actttaaaaa cattacctta atcattgtct tttcttcttg
     4621 aagtctttcc cagtgaaaac gctcaattct gctgtttcca tagaattttt aatttatttt
     4681 aagacatgag attgtaaaca aattgcttga tttattttat cctaattatt taaataaaat
     4741 caccctaaag catcaaaaaa aaaaaaaaaa aaa
//
