LOCUS       NM_028916               2638 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus EF-hand domain (C-terminal) containing 2 (Efhc2),
            mRNA.
ACCESSION   NM_028916 XM_006527705 XM_135814
VERSION     NM_028916.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2638)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   4  (bases 1 to 2638)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   5  (bases 1 to 2638)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
REFERENCE   6  (bases 1 to 2638)
  AUTHORS   Gifford,J.L., Walsh,M.P. and Vogel,H.J.
  TITLE     Structures and metal-ion-binding properties of the Ca2+-binding
            helix-loop-helix EF-hand motifs
  JOURNAL   Biochem J 405 (2), 199-221 (2007)
   PUBMED   17590154
  REMARK    Review article
REFERENCE   7  (bases 1 to 2638)
  AUTHORS   Setter,P.W., Malvey-Dorn,E., Steffen,W., Stephens,R.E. and
            Linck,R.W.
  TITLE     Tektin interactions and a model for molecular functions
  JOURNAL   Exp Cell Res 312 (15), 2880-2896 (2006)
   PUBMED   16831421
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX004977.8, AK029501.1, AK016714.1 and BE956904.1.
            
            On or before Dec 2, 2017 this sequence version replaced
            XM_006527705.3, NM_028916.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.249338.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849381,
                                           SAMN00849384 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                BX004977.8         72886-72953         c
            69-103              BX004977.8         72346-72380         c
            104-1770            AK029501.1         2-1668
            1771-2298           AK016714.1         1662-2189
            2299-2638           BE956904.1         1-340               c
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 12.34 cM"
     gene            1..2638
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /note="EF-hand domain (C-terminal) containing 2"
                     /db_xref="GeneID:74405"
                     /db_xref="MGI:MGI:1921655"
     exon            1..68
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            69..195
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             154..2406
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /codon_start=1
                     /product="EF-hand domain-containing family member C2"
                     /protein_id="NP_083192.2"
                     /db_xref="CCDS:CCDS40883.1"
                     /db_xref="GeneID:74405"
                     /db_xref="MGI:MGI:1921655"
                     /translation="MALPFLPGNSFNRNIGKERFHKSQHWGFCNNVRMLVSENKPGVG
                     GDLLYGQKIKPKHSVFPKGDGTDAPSWVAFDKQVLSFDAYLEDEISDKRQEIFRIRYY
                     KIYFYLEDDTIQVNEPEVINSGLPQGTSIRRQRIPYPPPNDDQFYTVYDFNINISVVF
                     YGRTFKIYDCDPFTKNFLKKIGIKLNPPGQCPLDPYMKMRRETLEFVDPFRPYQSFDT
                     LKRFIQYDGKVLRFFCLWDDSTSLFGDRREFVLHYFLCDGTVEIREVLPSNSGRDAMS
                     SFLRRGKLPKYGPPGIYQPGQITDRAVLNVYGGLSEWRADGYLLDKYQLGKVEQDFYT
                     DQDLSIGATINVWGRKVLLCDCDEFTKTYYRTKYGVDNFTPISCKPPHLPKIERKYPP
                     YTGFGSEEDSFRSCVGLKPTPHRKNFKKFMELDSFGNISNILRYFGKLITHKCADVDR
                     IFVIAFYLSDDTISVFEPIENNSGNAGGMFLKRSRVKKPGQEVFKSEFSEYIKAEELY
                     IGATVNINGYLFILLNADEYTLNYMENNTDKFPYSNFELAIQKLKQEKSKSREITQVF
                     AAADYNHTKVVPYNTFRDILMSITMGKLIDQELITIARHYRVPEIMDPDLAYLIARAH
                     EKFKKNIFENFDMFIYNCVYEDREKKGVLPTKDIRRMCKSSRLPLDDDFLDCLLSRFE
                     DKDHQINYEIFFSVLNWRMNPTPDLQAPPYLKEKCEDVWVGMPSPIPVKYVRYLDFLI
                     DVYGLEDNML"
     exon            196..384
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            385..535
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..759
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            760..1011
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1125
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1264
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1430
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1573
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1770
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1901
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2099
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2192
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2298
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2622
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2601..2606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2622
                     /gene="Efhc2"
                     /gene_synonym="4933407D04Rik; mRib72-2"
                     /note="major polyA site"
ORIGIN      
        1 atacaagaaa ggatcagagg aagaactgaa ggccatgctt acctttggaa tgagtttccc
       61 agtagggggt aacgcagggg tcaaacaagc agcggttgag ggcattcatc tctctttggt
      121 tggaccctgc atcctcatcg gacgttgaca gccatggctc taccgtttct gcctggcaac
      181 agctttaacc gcaatatagg aaaggagagg ttccataaat ctcaacattg gggcttttgc
      241 aacaatgtca ggatgctggt gagtgagaat aagcctggag taggtggaga tctgctatat
      301 ggccaaaaga taaagcctaa acacagtgta tttcccaaag gagatggaac cgatgcacca
      361 tcctgggttg cttttgataa gcaggtgtta tcttttgatg cctatttgga agatgaaata
      421 tctgataaaa gacaagaaat ctttagaata agatactaca aaatctattt ctaccttgaa
      481 gatgacacca ttcaagtaaa tgaaccagaa gtgataaata gtggactgcc tcaaggcact
      541 tctatcaggc gtcaacggat tccttacccc cctcccaatg atgatcagtt ttatactgta
      601 tatgacttca atatcaacat aagtgttgtc ttctatgggc gcacattcaa gatttatgat
      661 tgcgatccat ttacaaaaaa ctttttgaag aaaataggaa tcaaattgaa ccctccagga
      721 caatgtccac tcgatcccta catgaagatg cggagagaga cattagagtt tgtggatccc
      781 tttcgtccct atcagtcttt tgacaccctg aaacgattca tccagtatga tgggaaggtt
      841 ttgcgtttct tctgcttgtg ggatgattca acctcactgt ttggggaccg tagagaattt
      901 gtcctgcatt acttcctgtg tgatggtact gttgaaatca gggaagtact gccatctaat
      961 tcaggccggg atgctatgtc atcattcctt cggcggggta agcttcccaa gtatggccca
     1021 cctggaatct accaaccagg tcagataaca gatcgagcag ttctcaatgt gtatggaggc
     1081 ttgtcagaat ggcgagcaga tggctatctg ctggataaat accagctagg aaaagtagag
     1141 caagactttt acacagacca ggacctgtct ataggtgcca ccatcaatgt atggggaaga
     1201 aaagtcctcc tctgtgactg tgatgagttt accaagacct attataggac aaagtatgga
     1261 gttgataact ttacgcccat ttcatgcaag cctcctcatc ttccaaagat agaaaggaaa
     1321 tacccacctt atactggctt tgggtctgaa gaggattctt tccgctcctg tgtaggcctg
     1381 aagcccacac ctcatcggaa gaacttcaaa aaattcatgg aactagacag ctttggaaac
     1441 ataagcaata tactccgata ttttggaaaa ctaattacac acaaatgcgc tgatgtggac
     1501 aggatctttg tcattgcatt ttatctcagt gatgacacaa tttcagtgtt tgagcctata
     1561 gagaataatt cagggaatgc tggtggaatg ttcttaaaaa gaagtcgtgt gaaaaagcct
     1621 ggacaagaag tttttaaaag tgaattttca gaatacatca aggctgaaga gctctatatt
     1681 ggagccacag tgaatattaa tggctacctg tttattctgc ttaatgctga tgagtacacc
     1741 ttaaactaca tggagaataa cacagacaag tttccatata gcaactttga acttgccata
     1801 caaaaactga agcaggaaaa atcaaaatca agagagatca cacaagtatt tgcggctgct
     1861 gactataatc acacgaaggt ggtaccttac aatacattca gagatatcct gatgagtatc
     1921 accatgggga agctcataga ccaggagctt ataaccattg cacgccacta ccgtgtgcct
     1981 gagattatgg atccagactt agcatactta attgcccggg cccatgaaaa gttcaagaaa
     2041 aatatatttg agaattttga catgttcatt tataactgtg tgtatgaaga cagagaaaaa
     2101 aaaggtgtat tacccaccaa agacatcaga aggatgtgca agtcttccag gctacctctg
     2161 gatgatgact ttctagattg cttactgtcc aggtttgaag acaaagacca tcaaataaat
     2221 tatgagatat ttttctccgt cctgaactgg agaatgaatc caacaccgga tttgcaagcc
     2281 ccgccatacc ttaaagagaa atgtgaagac gtttgggtgg gaatgccatc acctattcct
     2341 gtgaaatacg ttagatactt agactttttg attgatgtat atggcttaga ggacaacatg
     2401 ctatgaaggt tttgatcaac tctctttgat ttactgttct cattatgcca tgtttatttt
     2461 aataaatata atttcattta aaacaaacaa aaaataagaa acaaggtttg tattaaatgg
     2521 aaattcataa accacaatta attttctttg tcactgtagt tcctcagaac agcttgttat
     2581 attgttgatt taccagcact attaaaaata ccatttaaaa caaaaaaaaa aaaaaaaa
//
