LOCUS       NM_027031                767 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus EF-hand calcium binding domain 9 (Efcab9), mRNA.
ACCESSION   NM_027031
VERSION     NM_027031.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 767)
  AUTHORS   Zhao,Y., Wang,H., Wiesehoefer,C., Shah,N.B., Reetz,E., Hwang,J.Y.,
            Huang,X., Wang,T.E., Lishko,P.V., Davies,K.M., Wennemuth,G.,
            Nicastro,D. and Chung,J.J.
  TITLE     3D structure and in situ arrangements of CatSper channel in the
            sperm flagellum
  JOURNAL   Nat Commun 13 (1), 3439 (2022)
   PUBMED   35715406
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 767)
  AUTHORS   Hwang,J.Y., Wang,H., Lu,Y., Ikawa,M. and Chung,J.J.
  TITLE     C2cd6-encoded CatSpertau targets sperm calcium channel to Ca2+
            signaling domains in the flagellar membrane
  JOURNAL   Cell Rep 38 (3), 110226 (2022)
   PUBMED   34998468
REFERENCE   4  (bases 1 to 767)
  AUTHORS   Yang,F., Gracia Gervasi,M., Orta,G., Tourzani,D.A., De la
            Vega-Beltran,J.L., Ruthel,G., Darszon,A., Visconti,P.E. and
            Wang,P.J.
  TITLE     C2CD6 regulates targeting and organization of the CatSper calcium
            channel complex in sperm flagella
  JOURNAL   Development 149 (2) (2022)
   PUBMED   34919125
REFERENCE   5  (bases 1 to 767)
  AUTHORS   Lin,S., Ke,M., Zhang,Y., Yan,Z. and Wu,J.
  TITLE     Structure of a mammalian sperm cation channel complex
  JOURNAL   Nature 595 (7869), 746-750 (2021)
   PUBMED   34225353
REFERENCE   6  (bases 1 to 767)
  AUTHORS   Hwang,J.Y., Mannowetz,N., Zhang,Y., Everley,R.A., Gygi,S.P.,
            Bewersdorf,J., Lishko,P.V. and Chung,J.J.
  TITLE     Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates
            Sperm Motility
  JOURNAL   Cell 177 (6), 1480-1494 (2019)
   PUBMED   31056283
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC087911.1 and AV260313.1.
            
            On May 28, 2009 this sequence version replaced NM_027031.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087911.1, AK005716.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-748               BC087911.1         1-748
            749-767             AV260313.1         160-178
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 18.97 cM"
     gene            1..767
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /note="EF-hand calcium binding domain 9"
                     /db_xref="GeneID:69306"
                     /db_xref="MGI:MGI:1916556"
     exon            1..190
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..705
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /codon_start=1
                     /product="EF-hand calcium-binding domain-containing
                     protein 9"
                     /protein_id="NP_081307.2"
                     /db_xref="CCDS:CCDS24528.1"
                     /db_xref="GeneID:69306"
                     /db_xref="MGI:MGI:1916556"
                     /translation="MKLTPGCFLWYLYMDKIYCLLSLRNVKALMVYFHLLDVHHRNTL
                     NDVLFFHFLQHVTNLNKSQIGMIFDLLDWTAVGEIGFDQFYVLICILLAHQDHLEDHF
                     MYRHSRPVFELLDLDGEMNIGAANFQNYRFLFNIKKQELRDLFHDFDITGDRLLNYKE
                     FKLYTIFCTDKSIDRKKRRKDREAAREREKEKGKDKEKYLHLKKIYSSMLSHRSIL"
     exon            191..339
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            340..516
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            517..767
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      731..736
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /note="hexamer: AATGAA"
     polyA_site      751
                     /gene="Efcab9"
                     /gene_synonym="1700007I06Rik; 4930403C08Rik"
                     /note="major polyA site"
ORIGIN      
        1 agaattctcc attctgtatc aggttgccag agcctccggc tgaaagctgc cgccatgaaa
       61 ctgactccgg ggtgttttct gtggtacctt tacatggaca aaatctactg cttactgtct
      121 ttgagaaacg tgaaagcctt gatggtgtac tttcatcttc tggatgtgca ccacaggaac
      181 accttgaatg atgtgctctt ctttcatttc ctccagcacg tgactaacct gaacaagtca
      241 cagatcggga tgatatttga cctcctggac tggacagctg taggagagat tggttttgac
      301 cagttctatg tgttgatttg catactgctg gcacatcagg accatctgga agaccacttt
      361 atgtaccgcc attcccggcc agtctttgag ctgcttgatc tggacgggga gatgaatata
      421 ggtgcggcca atttccaaaa ctacagattt ctcttcaata ttaaaaagca ggaactccga
      481 gaccttttcc atgactttga catcacaggt gaccgactgc ttaattacaa ggaatttaag
      541 ctgtacacaa tcttctgcac cgacaaatcc atagacagaa agaaaaggag gaaagacaga
      601 gaggcagcga gagagagaga aaaagagaaa gggaaagata aagagaagta tcttcatctc
      661 aagaagatat attcatctat gttaagccac aggagtatcc tgtgacacac gagtgagcat
      721 ggataattaa aatgaacatg gccacaatga agtagagcat cgaagtg
//
