LOCUS       NM_001370916            4442 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Mus musculus EF-hand calcium binding domain 8 (Efcab8), mRNA.
ACCESSION   NM_001370916
VERSION     NM_001370916.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Lau,N.C., Seto,A.G., Kim,J., Kuramochi-Miyagawa,S., Nakano,T.,
            Bartel,D.P. and Kingston,R.E.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833803.8.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were inferred based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849384
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-180               AL833803.8         70915-71094
            181-222             AL833803.8         71600-71641
            223-411             AL833803.8         72560-72748
            412-463             AL833803.8         73433-73484
            464-629             AL833803.8         75310-75475
            630-748             AL833803.8         79303-79421
            749-852             AL833803.8         80825-80928
            853-988             AL833803.8         84377-84512
            989-1094            AL833803.8         86036-86141
            1095-1179           AL833803.8         89312-89396
            1180-1304           AL833803.8         90225-90349
            1305-1378           AL833803.8         94037-94110
            1379-1509           AL833803.8         96556-96686
            1510-1577           AL833803.8         101497-101564
            1578-1729           AL833803.8         101875-102026
            1730-1864           AL833803.8         102541-102675
            1865-1975           AL833803.8         104293-104403
            1976-2203           AL833803.8         106048-106275
            2204-2274           AL833803.8         108037-108107
            2275-2479           AL833803.8         111964-112168
            2480-2659           AL833803.8         114203-114382
            2660-2806           AL833803.8         115408-115554
            2807-3025           AL833803.8         123049-123267
            3026-3184           AL833803.8         123785-123943
            3185-3353           AL833803.8         130535-130703
            3354-3486           AL833803.8         132787-132919
            3487-3682           AL833803.8         134560-134755
            3683-3781           AL833803.8         134825-134923
            3782-4442           AL833803.8         135761-136421
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..4442
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /note="EF-hand calcium binding domain 8"
                     /db_xref="GeneID:100504221"
                     /db_xref="MGI:MGI:3644206"
     exon            1..180
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            181..222
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            223..411
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     CDS             245..4216
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /codon_start=1
                     /product="eF-hand calcium-binding domain-containing
                     protein 8"
                     /protein_id="NP_001357845.1"
                     /db_xref="CCDS:CCDS89569.1"
                     /db_xref="GeneID:100504221"
                     /db_xref="MGI:MGI:3644206"
                     /translation="MPSSLHAPLSGEVSAFSLSCPYFRCQDSTGEDLGRFPSPLPFLM
                     CLLLFFPFRARWPRLMSSEKPGESPKPQKMAQPGGSQKKETSRSVPVTDPTSHNSEIN
                     QRDQQFSDMHLADLQKVFEKEADENGALKKEGFIRIMKGVLSSMSEEMLELLFLKVDS
                     DCNGFVTWQKYVDYMMREFQGKEEMRKSQYRLRFHLPMTVIPLHHGSEVVKVACLTKR
                     FKKMGRFLTVTKDGILQFWSETFSMLESFHLSQSKQYGHQQMWVIDVAYLHNMSLIAV
                     ASTELKIEFFDIGGYKCIRAFTVIDLDSCVMVMDYWTDCHKAVFCYGDTKGNATIFIS
                     DNVAGGLFNPQILPRASKWDHWFTVSLKKLLNEKAPLYRSFRMKGLHPNWCQQVKYIP
                     QLNVVASCSAVEKSSLVLIILPGKEPEKPRLSSLNLRKGILCFDYCPDRNFLATGGYD
                     PHIRLWNPLVSKKPVWLMKGHQTSVTHLLVNSRNASILISISRDKNIRVWDLQDYVCL
                     QSFCGKLFPLGNCPITSTYFYRSSTLICSTYNIGILRGYLESQGPLKTGRMTTHNTPL
                     CAVLYSKIFKQVVSGCLQGLVSVWDISTGKKRMEIPVSGIQESELTAMALDQSERCLL
                     TGLRDGTMQMWNYNTGECLMTFPNPDRVEISGIVHMNKVFYTTGWSKRITNFTFQKIT
                     QALSCYHWQSFHTEDILSMDKYQNQFLGTSSYNGDIVFWNVSTSKPILSFNASKSPLP
                     LEPKKVRDKEICIPKTSIRLDYEDQKWAHRPWKSAISTGKRRLMSAPPVMKHWKDAEQ
                     QRNMAQEKHFRSHKPGLSKMLVNKSGNKLAFDKDTQKRKGDLWRKLLLQSSVSVEKIL
                     FLQTRPRLPHTAALLSSSIDGFIYAWSIHGNGGLLGKFSVDNEVGESVVGAMATDEND
                     WILLTGDSKGNIKIWDIKDYCYFIDQQPLQSSGNSLSVDTENKFRLLIPKQLQVCSEH
                     HLPQEEKEVVEGQTFSLVPPPLLITWNSHLDSVTDILYVNSFQLVVSAGQDRDVKAWR
                     LSGDAIGTFGLNLWKRLQEAAMVDELEQGASLEKKPATTGATLKHSFLEMDKELAEAL
                     VYQRREQAVLLAFLRGRAEKETEAWSKLQQMSLTSPWTKSPSVEDIEESWTRWTKDKQ
                     ASKIVGAAYKPKVRVRNPKLLTSRAQFNWMRQQISPQVYQCLSFTDLKPIQQPDFMMH
                     KILDQVGRHIRWVASDMRSDMEAFRGQIPTDMVAGSPAATPSSLSMVSVGQSPSNWST
                     SLKPLSSTSFLRPSSASPAHSGSSTPPHILGLPRRGQRPQGQMLSPSCSTRSPGEPPS
                     CPL"
     exon            412..463
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            464..629
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            630..748
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            749..852
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            853..988
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1094
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1179
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1304
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1378
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1509
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1577
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1729
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1864
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..1975
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2203
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            2204..2274
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2479
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            2480..2659
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2806
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..3025
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            3026..3184
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..3353
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            3354..3486
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            3487..3682
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            3683..3781
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     exon            3782..4442
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4414..4419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /note="hexamer: AATAAA"
     polyA_site      4442
                     /gene="Efcab8"
                     /gene_synonym="A630008I04; EG329541; Gm38434"
                     /note="major polyA site"
ORIGIN      
        1 agacgaggtt aacgtctcat ctcgcacagc tgtgccttct gagcccttcc cctggagaca
       61 ttgttctctg tgcccccctt tgcttctgct ctgttatcct tccttgattc tcgtagagga
      121 gcttgggagt gcgctccctg ttcctaaaag gactccaaac cgtatttccg ttcttccaag
      181 agttcacaac catctggttc tgtctgcatt cccttcctca agatgagtcg tcacagcttg
      241 cttcatgccc tcgtccctcc atgctcctct ttctggagaa gtttctgcct tttctcttag
      301 ttgtccttac tttcgatgtc aagacagcac gggggaagac ctgggccggt tcccctcccc
      361 actgcccttt ctcatgtgcc tactgctctt cttcccattt agagccagat ggccaagact
      421 aatgtcttct gagaagccag gagagagccc caaacctcaa aagatggccc aaccaggtgg
      481 atcccagaag aaggagactt ccagatctgt tccagtgaca gatcccacca gccacaacag
      541 tgaaatcaac caaagagatc agcagttttc cgatatgcac ctggctgacc tacagaaggt
      601 gtttgagaag gaggcagacg agaatggagc cctgaaaaag gaaggcttca tcaggatcat
      661 gaagggggtg ctgagcagca tgtcggaaga gatgctcgag ttgctgttct tgaaggtgga
      721 ctcagactgc aatggctttg tcacttggca gaagtatgtg gattatatga tgcgtgagtt
      781 ccagggcaag gaggagatga ggaagagcca gtatcgcctg cgcttccacc ttcccatgac
      841 tgtcatcccc ctgcaccatg ggagcgaggt tgtaaaagtg gcatgtttaa ccaaacggtt
      901 caaaaagatg gggcgtttcc tgactgtcac caaagatggg atcctgcagt tctggtctga
      961 gaccttctca atgttggaat ccttccacct ttcccagtcc aagcagtacg gccaccagca
     1021 gatgtgggtt atcgacgtgg catatctgca caacatgagc ctcattgcag ttgcctccac
     1081 tgagctgaag atagaattct ttgatattgg tggctacaaa tgtatccggg ccttcaccgt
     1141 tattgatctg gatagctgtg tcatggtcat ggactattgg accgactgtc acaaagctgt
     1201 gttctgctat ggggacacca agggcaatgc caccatcttc atctctgaca acgtggctgg
     1261 tgggctgttc aatccacaaa tccttccccg ggcctccaaa tgggatcact ggttcactgt
     1321 ttccctgaag aagctcttaa atgagaaggc tcctctgtat agaagcttcc gcatgaaggg
     1381 tctccacccc aactggtgcc agcaggtcaa gtacatccca cagctgaatg tcgtggcctc
     1441 gtgttcagcc gtggagaagt cctctctggt gctgataata ctgccaggca aagaacccga
     1501 gaaacccagg ttatcatcac tgaatttaag aaaaggaatt ctttgctttg actactgtcc
     1561 agacaggaac tttctggcaa ctggtggcta tgacccccac atccgcctgt ggaaccccct
     1621 ggtctcaaaa aagcctgtgt ggctgatgaa gggacaccag acatcggtga cacacctcct
     1681 tgtgaacagc aggaatgcca gcatcctgat cagcatttct agggacaaga acattcgagt
     1741 gtgggatctg caggattatg tgtgcctcca gtccttctgc ggaaagctct ttcccctggg
     1801 caactgcccc ataaccagca cctacttcta caggagcagc accctcatct gtagcaccta
     1861 caatattggg atcctcagag gatacctgga atcccagggc cccctgaaaa cagggaggat
     1921 gacgactcac aacacacccc tgtgtgcagt cctctacagc aagatcttta agcaggtggt
     1981 gagtggctgc ctccaggggc tagtgagtgt gtgggacatc agcacgggca agaagaggat
     2041 ggagataccc gtgtccggca tccaggaaag tgagctgacg gccatggctc tggaccagtc
     2101 ggagaggtgt ctgctcacgg gcttgcgtga cggcacgatg cagatgtgga attacaacac
     2161 cggggagtgc ctgatgacct ttcccaaccc ggacagagtg gagattagtg ggattgtcca
     2221 catgaacaaa gtgttctaca cgaccgggtg gagtaagaga atcaccaatt tcacgttcca
     2281 aaagatcaca caggcgctct cctgctacca ctggcagagt ttccatacag aggacatcct
     2341 gagcatggac aagtatcaga atcagttcct cgggacctct tcctacaatg gggacattgt
     2401 tttctggaac gtcagcacgt caaagcccat cttgagtttc aatgcctcca agagcccctt
     2461 gcctttagag ccaaagaagg tgagagataa agagatatgc atacccaaga cttcaatccg
     2521 tctagactat gaagatcaaa agtgggcaca caggccctgg aagtcagcta tctccactgg
     2581 gaagcggaga ctgatgtcag ctcccccggt gatgaaacat tggaaggacg cagagcaaca
     2641 gaggaatatg gctcaggaga aacatttcag gagtcacaaa cctggattgt ctaaaatgct
     2701 tgtcaataag tcgggtaata aactggcgtt tgataaagac acccaaaaga gaaagggaga
     2761 cttatggagg aagttgctgt tgcagtccag tgtgtcagtg gagaagatcc tttttctgca
     2821 gaccaggccc cgcctgccac acacggctgc cttgctgagc agctctattg acggtttcat
     2881 ctacgcctgg tccatccatg ggaacggagg gctgctggga aagttctccg tggacaatga
     2941 agttggtgag agtgttgtgg gtgccatggc cactgatgag aacgactgga tcctcctcac
     3001 gggggacagt aaaggaaata tcaagatctg ggacatcaag gattactgct actttattga
     3061 ccagcaaccg ctccaatcca gtgggaactc actctccgtt gacacagaga ataagttccg
     3121 gctcttaatt cctaagcaac tccaggtctg ctccgagcac cacttgccac aggaggagaa
     3181 ggaggttgtg gaaggacaga ccttttctct ggttccccca ccacttctga ttacctggaa
     3241 cagccacctg gacagcgtga cagacatcct gtatgtgaac tccttccagc tggtggtcag
     3301 tgcgggccag gaccgggatg tcaaggcttg gagactctct ggtgatgcca taggaacatt
     3361 tggcctgaat ctctggaaga gactgcaaga ggctgcgatg gtggatgaac tggagcaggg
     3421 ggcaagtttg gagaagaagc ctgctacaac cggtgccacc ctgaagcact ccttcctaga
     3481 gatggacaag gaattggctg aagccctggt gtaccagcgg cgagagcagg cagtgctgtt
     3541 agcctttctg cgtgggagag ctgagaaaga aactgaggca tggtccaagc tgcagcagat
     3601 gtccctcaca tctccatgga ccaaatcgcc ttctgttgaa gacatcgagg agagctggac
     3661 tcgatggacc aaggacaagc aggcaagcaa aattgtgggg gcagcataca agcccaaggt
     3721 gcgtgttcgg aaccccaaac tccttacctc cagagctcag tttaactgga tgagacagca
     3781 gatctctcct caggtctacc aatgcctgtc cttcactgac cttaagccga tccaacagcc
     3841 cgacttcatg atgcacaaga tcctggacca agtaggccgg cacatccgct gggtggccag
     3901 tgatatgcgg agtgacatgg aggctttccg gggtcagatc cccacagaca tggtggccgg
     3961 ctcacctgct gccacaccct cctccctctc catggtttcc gtgggacaat caccctccaa
     4021 ctggtctacc tccctgaagc ccctttcctc cacctccttc ctcaggcctt catcagcctc
     4081 tccggcccat tctggttctt ctacgccacc gcatatcctg ggcctgcccc gaagaggtca
     4141 gagaccccaa ggccagatgt tatctccttc ttgcagcacc cgaagcccgg gggagccacc
     4201 ctcgtgtccc ctgtgaggtg gagctcccac ctcatgtgtt gaggtgttca ttattttctc
     4261 tgttctgtag caggggacct atagggtctt gcacgtccct ggctcgctct ctgccaggct
     4321 gggaggatgc agccccttcc ttcccatact gcttctctct agataactcc agctgtcttg
     4381 tggctctgca cagttctgga gaaggaaaaa aaaaataaat attctcctgt gttagagtcc
     4441 ta
//
