LOCUS       NM_026626               3665 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus EF-hand calcium binding domain 2 (Efcab2), mRNA.
ACCESSION   NM_026626
VERSION     NM_026626.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Shawki,H.H., Ishikawa-Yamauchi,Y., Kawashima,A., Katoh,Y.,
            Matsuda,M., Al-Soudy,A.S., Minisy,F.M., Kuno,A., Gulibaikelamu,X.,
            Hirokawa,T., Takahashi,S. and Oishi,H.
  TITLE     EFCAB2 is a novel calcium-binding protein in mouse testis and sperm
  JOURNAL   PLoS One 14 (4), e0214687 (2019)
   PUBMED   30933994
  REMARK    GeneRIF: EFCAB2 is a novel calcium-binding protein in mouse testis
            and sperm.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3665)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   4  (bases 1 to 3665)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157809.4.
            
            On Apr 11, 2018 this sequence version replaced NM_026626.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029880.1, BC025062.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               AC157809.4         30463-30578
            117-201             AC157809.4         61847-61931
            202-274             AC157809.4         80261-80333
            275-354             AC157809.4         99327-99406
            355-397             AC157809.4         100130-100172
            398-464             AC157809.4         100270-100336
            465-3665            AC157809.4         105758-108958
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 83.16 cM"
     gene            1..3665
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="EF-hand calcium binding domain 2"
                     /db_xref="GeneID:68226"
                     /db_xref="MGI:MGI:1915476"
     exon            1..116
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..580
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="EF-hand calcium-binding domain-containing protein
                     2"
                     /codon_start=1
                     /product="dynein regulatory complex protein 8"
                     /protein_id="NP_080902.1"
                     /db_xref="CCDS:CCDS15557.1"
                     /db_xref="GeneID:68226"
                     /db_xref="MGI:MGI:1915476"
                     /translation="MAEERDAEGTEALIAELHKKIKDAFEVFDHESNNTVDVREIGTI
                     IRSLGCCPTEGELHDFIAEIEEEEPTGYIRFEKFIPVMTRALVERRYRPAAEDILLRA
                     FEVLDPAKRGFLTKDELVKYMTEEGEPFSQEEMEEMLSAAIDPESNTINYRDYITMMV
                     VDEN"
     exon            117..201
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            202..274
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            275..354
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            355..397
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            398..464
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            465..3665
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      576..581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      612
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
     polyA_site      668
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
     regulatory      764..769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: CATAAA"
     polyA_site      788
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
     regulatory      1126..1131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      1150
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
     regulatory      3643..3648
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="hexamer: AACAAA"
     polyA_site      3665
                     /gene="Efcab2"
                     /gene_synonym="1700073K01Rik; D830011E08Rik"
                     /note="major polyA site"
ORIGIN      
        1 accgtgcatc caggggcttt ctccgcccgc ggggtcccgc agggttcgtg ctggatgtct
       61 cccgggcgac cggctccgca ccaagatggc ggaggaaagg gacgcggaag ggacagaggc
      121 actaattgca gaacttcata agaagatcaa agacgcattt gaagtgttcg accatgaatc
      181 gaataataca gtagatgtga gagagattgg gacaattatc aggtcattag gatgctgccc
      241 gactgaagga gagctgcatg atttcattgc agagatagag gaagaagaac ccactggcta
      301 cattcgattt gaaaaattta ttccagtgat gaccagagcc cttgtagaaa gaagatacag
      361 accagctgct gaagatatcc ttttgcgagc tttcgaggtt ttggatccag ctaaacgcgg
      421 gtttctcact aaggatgaac ttgtcaagta catgactgag gaaggtgagc ctttttccca
      481 agaggaaatg gaagaaatgt tgtctgctgc aattgatccc gaatcaaaca ccattaatta
      541 tagggactac ataacaatga tggtggtaga tgaaaattaa atgctgcaaa gacttctttt
      601 ttctcctttt aagacaactg taaacattca atttctagtt gaaatgataa tttgaaaatt
      661 tgtcctaatt aatttcacat tttattgtat aatttctaca tttagtgcct tatgacataa
      721 ttgtttcaag gtattttgtt tactagagat gatcagttta aaacataaat ttatttagta
      781 tgaccagatt tatggaagga caagttaatt atttcaaagg aattgtacag tattttaatg
      841 ttaaatgtat cgttgcttat tttccacaac taggccaatg atacattgaa actaattaga
      901 aagtgagatt gatcctaacc ccctaacatt tgccatagaa agctcttgca gggcaggatg
      961 gccacagact agatgtgagt ggcagcttgg tgatggctgc ctttcagagc agcagtaaac
     1021 acttcagcaa gcagaatatg gagggagagc gaccagaata actattaata attgatttct
     1081 tatgaatgcc aattacaaat ctcatttgag agtctaataa ctttgagtaa aattgaaaat
     1141 tgctcatcca gcattttgcc atttatggaa tagctaaagg aggtgagata attggatcca
     1201 gagaaaggaa gactgaatga aagctgataa tggcaacttc tttttttgct ctttagaatt
     1261 ctcacctatt cagaatatct gtcatgggaa tattgtcctg gatactgcgt gtattcatct
     1321 gttgttacca gaatgtagta tctgataagg gctgactcca gaagaaagag attcatttag
     1381 ctcacagtta tagatggtca aggtccaggc agcattgtac aggctgagct agggccaccc
     1441 gtgtatgcat ctcaccaagg tacagctagc cagacagaag cacaagcaag caagagcaga
     1501 gaggctggga ggaggggtca ggcctgttcc ttttacaata gtcctctcat aagaattact
     1561 ttccaggctg gggagatggc tcaatgggta aagcatttgc tgagtatcat tagataatgt
     1621 ggtggaagct ggagatgaaa gatggataaa cctctaatta atcaaggtca cagtttagtg
     1681 agggagtctt gcaagtaagt caatgagcaa ccacacattc aggggactga aggttgcaca
     1741 aaagaagcaa aagaagcctg gtgggatgag ggtgctttct ggtgggcaga ctcaaagagg
     1801 taacacatga cttacaaact cagtagttct taagttttga caaatagtca ttctttgagc
     1861 agtttacttc agagcatgtc agcagcagga cagcagatca aacccccaga ggtgtcctga
     1921 aacgcaagaa tctgggattg aaagtatggt tgatagatat ttggaacctg atgagagatg
     1981 agaggttgaa ataagaacca tatcctaaga gataatgagc taattggggt taaggtttca
     2041 gtgtttgaac gtgaactttc tctgagaatc agagagaaag gagctggagt tgtaaatagc
     2101 ctgagaaata ctttgaaggg tcaaggccag agggaaggag accagctggt aggctgcttc
     2161 gttggcagtg agagatgggc tagggaaagt caagcacaaa tcctaagtgt ctggcttgaa
     2221 cacttggtaa ataataatat cagccattca gataagatgc ccaaggctta ggctaatagg
     2281 cttgactgtg gtcatctcca gcttgaagag ctggtagaag ataaaaagat caagtggata
     2341 aataagggga catctgctag agacacaggc tttgaagttg ttagcacacg gtgttctgga
     2401 aagaaaataa aagatgacca ggacagaact gggagtacag aagaggaccc ctcagagcat
     2461 ggggagaagc cctgaaagga ggaaaagctg aagaaagaga atccatggca gtagagtctc
     2521 ttaaggaaga aggaaccaag tcagaagtgg tagagttgtg aggtcgctga tgatgaggaa
     2581 atccctcagt ttcagcacca acacatccac tgagaacctc tctcagggca gatttgtgaa
     2641 gtggcaggca ggagagttag tgacttcagg aataaacaga tgtgaggaag agcctggagg
     2701 acacgtgtcg agtggtgaag agagagaagg tagaggggag gggtttggaa gatgtaaacg
     2761 gtgcaagaat gctgcaagct tgtatttcaa gagttgagac atggcagagg agcagaggac
     2821 actcagaggg gcagagatcc agggctacaa aggtcttgac cttggtcatg gacatgaact
     2881 aatgtatata gtccctgcct agtggcttct agctcttata acgtgtgacc tctctgcttc
     2941 tgcctcccta gtgctaggat tctaggcctg aacctctgca cccagttata ctgtctctat
     3001 gaatgagaag gaagatagta ggttatagag ctagaggaga tgatagaaat ctgaagggct
     3061 ttggggcttt gtgagaaatg agagagttgg ggaataatct acaaaagaat tttcaaggat
     3121 tttgtccagt aacactggcc cagaagcaaa atatgtagag tgttgtagtg agtcaagatg
     3181 gtgaaatgaa cagacaaaaa aaaaaaaaaa aaaaaaaaag atcaagattg atgagtcagc
     3241 agaaatgagt gaccttgatg gtctgtaggc aaaggtggga gggaaatcca gctaggagta
     3301 gacttgcatg gaagattgta agactccaaa gggcaaacag tgtgtggtga gaaggcctgt
     3361 cattcctgag aacaatatgc tctgcttgca gtttgacttc tctgtactat tactgagcat
     3421 ttctctgtgt tattattaat tagtaatagt atacagtgta tatacatctc tcactatctg
     3481 tcaggcactg ttaagtgttt tacttaacct actcatttaa aattcctgat gacccagtga
     3541 agtagttacc attactatga gggagtggca aagaagtttt gtaactataa gggactgcat
     3601 taatcagcct ttgtaggctt tgtgatgctg aatcttcacc acaacaaaac taatttttca
     3661 tgtca
//
