LOCUS       NM_023060               2645 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus eukaryotic elongation factor,
            selenocysteine-tRNA-specific (Eefsec), transcript variant 1, mRNA.
ACCESSION   NM_023060
VERSION     NM_023060.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Dubey,A. and Copeland,P.R.
  TITLE     The Selenocysteine-Specific Elongation Factor Contains Unique
            Sequences That Are Required for Both Nuclear Export and
            Selenocysteine Incorporation
  JOURNAL   PLoS One 11 (11), e0165642 (2016)
   PUBMED   27802322
  REMARK    GeneRIF: This study examined the subcellular localization of eEFSec
            in the context of altered Selenocysteine incorporation to
            demonstrate that reduced selenoprotein production does not
            correlate with changes in the nuclear localization of eEFSec.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Gonzalez-Flores,J.N., Gupta,N., DeMong,L.W. and Copeland,P.R.
  TITLE     The selenocysteine-specific elongation factor contains a novel and
            multi-functional domain
  JOURNAL   J Biol Chem 287 (46), 38936-38945 (2012)
   PUBMED   22992746
  REMARK    GeneRIF: SBP2/SECIS complex activates eEFSec by directing
            functional interactions between Domain IV and the ribosome to
            promote Sec-tRNA(Sec) binding and accommodation into the ribosomal
            A-site
REFERENCE   3  (bases 1 to 2645)
  AUTHORS   Danciger,M., Yang,H., Ralston,R., Liu,Y., Matthes,M.T., Peirce,J.
            and Lavail,M.M.
  TITLE     Quantitative genetics of age-related retinal degeneration: a second
            F1 intercross between the A/J and C57BL/6 strains
  JOURNAL   Mol Vis 13, 79-85 (2007)
   PUBMED   17277741
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2645)
  AUTHORS   Small-Howard,A., Morozova,N., Stoytcheva,Z., Forry,E.P.,
            Mansell,J.B., Harney,J.W., Carlson,B.A., Xu,X.M., Hatfield,D.L. and
            Berry,M.J.
  TITLE     Supramolecular complexes mediate selenocysteine incorporation in
            vivo
  JOURNAL   Mol Cell Biol 26 (6), 2337-2346 (2006)
   PUBMED   16508009
REFERENCE   5  (bases 1 to 2645)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 2645)
  AUTHORS   Zhang,S., Lloyd,R., Bowden,G., Glickman,B.W. and de Boer,J.G.
  TITLE     Thymic lymphomas arising in Msh2 deficient mice display a large
            increase in mutation frequency and an altered mutational spectrum
  JOURNAL   Mutat Res 500 (1-2), 67-74 (2002)
   PUBMED   11890935
REFERENCE   7  (bases 1 to 2645)
  AUTHORS   Smits,R., Hofland,N., Edelmann,W., Geugien,M., Jagmohan-Changur,S.,
            Albuquerque,C., Breukel,C., Kucherlapati,R., Kielman,M.F. and
            Fodde,R.
  TITLE     Somatic Apc mutations are selected upon their capacity to
            inactivate the beta-catenin downregulating activity
  JOURNAL   Genes Chromosomes Cancer 29 (3), 229-239 (2000)
   PUBMED   10992298
REFERENCE   8  (bases 1 to 2645)
  AUTHORS   Fagegaltier,D., Hubert,N., Yamada,K., Mizutani,T., Carbon,P. and
            Krol,A.
  TITLE     Characterization of mSelB, a novel mammalian elongation factor for
            selenoprotein translation
  JOURNAL   EMBO J 19 (17), 4796-4805 (2000)
   PUBMED   10970870
REFERENCE   9  (bases 1 to 2645)
  AUTHORS   Tujebajeva,R.M., Copeland,P.R., Xu,X.M., Carlson,B.A., Harney,J.W.,
            Driscoll,D.M., Hatfield,D.L. and Berry,M.J.
  TITLE     Decoding apparatus for eukaryotic selenocysteine insertion
  JOURNAL   EMBO Rep 1 (2), 158-163 (2000)
   PUBMED   11265756
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153694.1 and AC153927.6.
            
            On Aug 25, 2022 this sequence version replaced NM_023060.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147924.1, SRR13861889.63795.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-302               AC153694.1         21221-21522
            303-510             AC153694.1         91317-91524
            511-607             AC153694.1         109086-109182
            608-772             AC153694.1         112081-112245
            773-1432            AC153694.1         169492-170151
            1433-1589           AC153927.6         205827-205983       c
            1590-2645           AC153927.6         181614-182669       c
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 39.32 cM"
     gene            1..2645
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /note="eukaryotic elongation factor,
                     selenocysteine-tRNA-specific"
                     /db_xref="GeneID:65967"
                     /db_xref="MGI:MGI:2137092"
     exon            1..302
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1780
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /note="isoform 1 is encoded by transcript variant 1;
                     MJ0495-like protein SelB; selenocysteine-specific
                     elongation factor; elongation factor sec"
                     /codon_start=1
                     /product="selenocysteine-specific elongation factor
                     isoform 1"
                     /protein_id="NP_075547.1"
                     /db_xref="CCDS:CCDS20335.1"
                     /db_xref="GeneID:65967"
                     /db_xref="MGI:MGI:2137092"
                     /translation="MAGRRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRE
                     RGITLDLGFSCFVVPLPGAEPGSSDTLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLV
                     IDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLAEGKRQAAIDKMTKKMQKTLENT
                     KFRGAPIIPVAAKPGGPEAPETEAPQGISELIELLKSQISIPTRDPSGPFLMSVDHCF
                     SIKGQGTVMTGTILSGTISLGDSVEIPALKVVKKVKSMQMFHTPVTSAMQGDRLGICV
                     TQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRT
                     LFFSPAPDSFDLEPVLDSFDLSREYLFQEQYLCKDSMPTATEGDDEADPKAGHAPGGH
                     CPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGVLLQGLEDKNYIESF
                     LPALRVYKLKHKHGLVERVMDDYSVIGRSLFKKETNIQLFVGLKVQLSTGEQGIIDSA
                     FGQSGKFKIHIPGGLSPESKKILTPTLKKRSRAGRGETTKPEEGTERPEPIQPVTLNL
                     SFKRYVFDTQKRMVQTP"
     exon            303..510
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /inference="alignment:Splign:2.1.0"
     exon            511..607
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /inference="alignment:Splign:2.1.0"
     exon            608..772
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /inference="alignment:Splign:2.1.0"
     exon            773..1432
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1589
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..2645
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2092..2097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /note="hexamer: AATAAA"
     polyA_site      2111
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /note="major polyA site"
     regulatory      2627..2632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /note="hexamer: ATTAAA"
     polyA_site      2645
                     /gene="Eefsec"
                     /gene_synonym="mSelB; sec; Selb"
                     /note="major polyA site"
ORIGIN      
        1 aggcgacggc gtgagaggca gcggcgggat ggccggccgg cgggtaaatg tgaatgtggg
       61 cgtgctgggt cacatcgaca gtggcaaaac agcgctggcg cgcgcgctga gcaccacggc
      121 ctccactgct gccttcgaca agcagccgca gagccgcgag cgcggcataa cgctcgacct
      181 gggcttctcc tgttttgtcg tgccgctgcc gggggcagaa cccggttcta gcgacacgct
      241 gctgcaggtc acactggtcg actgccccgg gcacgcctcc ctcatccgga ccatcattgg
      301 cggtgcccag atcattgatt tgatgatgct cgtgattgat gtgaccaagg gaatgcagac
      361 acagtccgca gagtgccttg tgattggcca gatcgcctgc cagaagctgg ttgtggtgct
      421 gaacaaaata gacctgttag cagaagggaa gagacaggca gcgattgaca aaatgaccaa
      481 gaaaatgcag aagactcttg agaacaccaa attccggggt gcaccgatta ttcccgtggc
      541 agccaaacct gggggaccag aagcccctga aactgaagct ccacagggca tctcagaact
      601 cattgagctc ctgaagtccc agatttccat cccaacgaga gacccctctg gaccattcct
      661 tatgtctgtg gaccactgct tctccatcaa gggtcaaggc accgtgatga ctgggactat
      721 cctctcaggc actatcagcc ttggggacag tgtggagatc cctgccctca aggtggtgaa
      781 gaaagtgaag tccatgcaga tgttccatac acccgtcacc tctgccatgc agggggaccg
      841 actgggcatc tgtgtcaccc agtttgaccc caagctgttg gagcgtgggc tggtatgtgc
      901 acccgagtct ctgcacactg ttcacgcagc cctcatctct gtggagaaga tcccttactt
      961 ccgaggaccc ctgcagacca aggccaagtt ccatatcacc gttggccatg agacagtcat
     1021 gggtcgcacg ctgttcttca gccctgctcc tgacagcttt gacttggagc ccgtgctaga
     1081 ttccttcgac ctttcccgag agtacctctt ccaagagcag tacctgtgca aggattccat
     1141 gcccacagcc acagaaggtg atgacgaggc ggaccccaaa gccggccacg ccccaggggg
     1201 ccactgcccc cggcagcagt gggccctggt agagtttgag aagccagtca cctgcccccg
     1261 actctgcttg gtgattggct ccagactaga tgccgacatt cacaccaaca catgccggct
     1321 ggccttccat ggtgtcttgc tgcaagggct ggaggacaag aactacattg agagcttcct
     1381 gccagcactg agggtttaca agctgaagca caagcatggc cttgtggagc gggtaatgga
     1441 tgactacagc gtgattggac gttccctgtt caaaaaggag accaacatcc agctctttgt
     1501 ggggcttaag gtgcagctgt ccacagggga acagggtatc atcgacagtg ccttcggcca
     1561 aagtggcaag ttcaagatcc acatccctgg tggcctcagc cctgagtcca agaagatcct
     1621 gacacctaca ctcaagaagc ggagccgagc cggccgtgga gagaccacca agccagagga
     1681 gggcactgaa cggccagagc ccatccagcc tgtgacactc aacctgtctt tcaagcgcta
     1741 tgtctttgac acccagaagc gcatggtaca gactccctga atgaaccatc ctgcccagct
     1801 gcaagccaga ccacagtgcc agatgccccc agacatgcct caacctcccc agtctgaggc
     1861 agtcaacccc atcccactgc cattgcactg ttattgagcc ccattgagaa aacctgggga
     1921 aaatgaggaa cagtgccagg agggcctgcc actccatccc tgaacccact caggatagca
     1981 acgaggccta ctagaaaatg gccttagcag agccagcaac ctgggtcttc ccccgataag
     2041 agtgtgcagc cactcagcct tgccccaact agctggcacc ctccaacgga aaataaacat
     2101 cccatatgtg acctagtgtg cccatagtct gcctgtctcc cagggcttcc caggcatcac
     2161 agggagttgt gtgcattggg tttagggctc gagagctgct aggttcaacc aagtggctcc
     2221 tgggatgttg tcagtggacc ttgaagaatg gaggttttct gaacagctga gtttcctggg
     2281 gtcctctggc caggggttag taccctgggg ttccatcctt gcacgagagg tcaataggcc
     2341 ctggcctcca tttttggaac catggactcc caagggtgct agaccagggg tggagtacct
     2401 ggaccctctc catgctcatc ttcagcccca gcaccctcaa gcttagcatg gggactgaga
     2461 taggggcaag aggatagaac tgaccaacga aggaaatcag agcttttcca gacacctgta
     2521 aggggagcag atgtcccaat gtttctcggt ttggcctcca gacagacgtg aaggcctccc
     2581 tttatcccag taatcggtgc attatgcttt aattttcaaa aatggcatta aaaatattaa
     2641 caggg
//
