LOCUS       NM_027446               2140 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus eukaryotic elongation factor 2 lysine
            methyltransferase (Eef2kmt), mRNA.
ACCESSION   NM_027446 XM_489505 XM_622596
VERSION     NM_027446.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Francis,J.W., Hausmann,S., Ikram,S., Yin,K., Mealey-Farr,R.,
            Flores,N.M., Trinh,A.T., Chasan,T., Thompson,J., Mazur,P.K. and
            Gozani,O.
  TITLE     FAM86A methylation of eEF2 links mRNA translation elongation to
            tumorigenesis
  JOURNAL   Mol Cell 84 (9), 1753-1763 (2024)
   PUBMED   38508183
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2140)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 2140)
  AUTHORS   Phan,L.K., Lin,F., LeDuc,C.A., Chung,W.K. and Leibel,R.L.
  TITLE     The mouse mahoganoid coat color mutation disrupts a novel C3HC4
            RING domain protein
  JOURNAL   J Clin Invest 110 (10), 1449-1459 (2002)
   PUBMED   12438443
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK133590.1.
            
            On Apr 5, 2007 this sequence version replaced NM_027446.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133590.1, AK163493.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2140              AK133590.1         2-2141
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 2.51 cM"
     gene            1..2140
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /note="eukaryotic elongation factor 2 lysine
                     methyltransferase"
                     /db_xref="GeneID:70511"
                     /db_xref="MGI:MGI:1917761"
     exon            1..116
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1028
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /note="protein FAM86A; putative protein
                     N-methyltransferase FAM86A; family with sequence
                     similarity 86; eEF2-lysine methyltransferase"
                     /codon_start=1
                     /product="protein-lysine N-methyltransferase EEF2KMT"
                     /protein_id="NP_081722.1"
                     /db_xref="CCDS:CCDS27935.1"
                     /db_xref="GeneID:70511"
                     /db_xref="MGI:MGI:1917761"
                     /translation="MAPEDHEGATSLLQSFERRFLAARALPSFPWQSLEEKLKDPSGS
                     ELLLAILQRTVKHPVCVQHGPSVKYARCFLSKLIKKHEAVPTEPLDALYEALAEVLMT
                     QESTQCHRSYLLPSGNSVTLSESTAIVSHGTTGLVTWDAALYLAEWAIENPAAFTDRT
                     ILELGSGAGLTGLAICKACCPRAYIFSDCHAQVLEQLRGNVLLNGFSLEPHTPIDAGS
                     SKVTVAQLDWDEVTASQLSAFQADVVIAADVLYCWEMTLSLVRVLKMLEDCQRKSAPD
                     VYVAYTIRSQDTGKLFIEELDRAGIYWEEVPPHTGKLFPYEEHSAIVILKLVLTSRHG
                     V"
     exon            117..179
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     exon            180..260
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     exon            261..362
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     exon            363..496
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     exon            497..762
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     exon            763..909
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     exon            910..2140
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1399..1404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /note="hexamer: AATATA"
     polyA_site      1419
                     /gene="Eef2kmt"
                     /gene_synonym="5730409G15Rik; eEF2-KMT; Fam86; Fam86a"
                     /note="major polyA site"
ORIGIN      
        1 aaccctggcc ctgccccgcg atggcgcctg aggaccacga gggggccacg tctctgctgc
       61 agagtttcga gcgccgcttc ctggcggctc gcgcgctgcc ctcctttccc tggcagagcc
      121 tagaagaaaa actaaaggac ccctcgggtt ctgagctgtt gctggctatt ctgcagagga
      181 ctgtgaagca ccctgtgtgt gtgcagcacg gaccttctgt gaagtatgcc cgctgctttc
      241 tctcaaagct catcaaaaag catgaggctg tacccacgga gcctttggat gcactgtatg
      301 aggcgttggc agaggttctg atgacccaag agtccaccca gtgccaccgg agctatttgc
      361 tgccttcagg aaactcagtt acactctctg agagcactgc catcgtgtcc catggcacca
      421 caggcttggt tacatgggac gctgctctct acctggcaga atgggccatt gagaacccag
      481 cagccttcac tgacaggact atcctggagc taggcagtgg agctggcctt acaggcctgg
      541 caatttgcaa ggcatgctgc cccagagcat acatcttcag cgactgtcat gcccaagtgc
      601 ttgagcagct ccgtggaaac gttctcctca acggcttctc cttagagccg cacaccccca
      661 ttgacgcagg cagctcgaag gtgacagtgg ctcagctgga ctgggacgag gtgacagcct
      721 ctcagctctc tgccttccag gcagatgttg tcattgcagc agatgtactg tactgctggg
      781 agatgacact gtcactggtc agggtcctga agatgctcga ggactgccag aggaagagcg
      841 ctcctgatgt ctatgtagcc tatactatcc gcagccagga cacaggcaag ctgttcatcg
      901 aagagctgga ccgtgctgga atctactggg aagaggtgcc ccctcacact ggcaaactgt
      961 tcccttacga agagcattca gccatcgtaa tcctgaagct ggtgctgacc agtcgccatg
     1021 gtgtttgaag gtgatttgaa aaatgagatg actagccggg tatggtggcg cacgccttta
     1081 gtcccagcac tcgcgcagag gcaggtggat ttctgagttc aaggccagcc tggtctacaa
     1141 agtgagttcc aggacagcca gggctacaca gagaaaacct gtctcaaaaa accaaaaaaa
     1201 aaaaggaaag aaaaggaaag aagagaaaag aaaaatgact gtcatgggaa agcggaaagc
     1261 ggaaaggctt ctgttgagca ggaatgttaa ataacagcac aagtgtacag taagcacagg
     1321 ctgagtgctg gtgcctgtcc tcagcagctc atctcgagtg gacacaggct cagagcaaac
     1381 acttccattg tacaaataaa tatatacatt tattaagtat gactgtcctg tcagacagtt
     1441 cagccttgct cagccaaacc cagtgaacac aggctatccc agagctcaca gcagaaccag
     1501 aacgcaaggg atgtgacaag tccagaaaca aaaacccagg gactgagaag ttggcaggga
     1561 cactggaagt aacagaagtg ctcagaacag aagagagctg ccagatcagt agccagatcc
     1621 tagctgtcgc ccactgctgt cctcaggctt atgaccacta gggggcactc aaaagccccg
     1681 gcaggaaggg tggccacagg gtggatgggg cctgggggtc atctgtgagt gagcgagcag
     1741 agggagcaca gtgtgctgcc agctctcatc ccagcgttgc tgccccgact cctgcagttt
     1801 cttccggaac tggcttagct ttccagcagg gtctgggaac tttgagaaaa gcatctaggg
     1861 aaacaggaaa actggaactc agctccctgc accaggcccc ggcctccttg ctcgtgtcca
     1921 gtggtgcccc tccgctcact cgacacttga tggattcatc tactcaccca cagccctgcc
     1981 ctagagaatg gctagcctcc acctgctatt ctctcagttt cccctcccat cctcaacaca
     2041 cccctgcaga tgcagttaag atggaagcct ttagtgtgtc tatcagggac gggttgggtc
     2101 ctgcccacct gcctttgtaa ataaagtttt attggaactc
//
