LOCUS       NM_177941               3436 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus Eef1akmt4-endothelin converting enzyme 2 readthrough
            (Eef1ece2), transcript variant 1, mRNA.
ACCESSION   NM_177941
VERSION     NM_177941.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc Natl Acad Sci U S A 99 (26), 16899-16903 (2002)
   PUBMED   12477932
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC115541.2, AF489570.1, AU080537.1, AC087898.19 and AF489572.1.
            
            Summary: This locus represents naturally occurring readthrough
            transcription between the adjacent genes eukaryotic translation
            elongation factor 1 alpha lysine specific methyltransferase 4
            (GeneID:110599566) and endothelin converting enzyme 2
            (GeneID:107522). The readthrough transcript representing this gene
            encodes a fusion protein that shares sequence identity with each
            individual gene product. [provided by RefSeq, Jul 2017].
            
            Transcript Variant: This variant (1, also known as 2a-2) represents
            the longer transcript and encodes the longer isoform (a, also known
            as 2a-2).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF489570.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 107522,
                                      110599566
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-193               BC115541.2         3-195
            194-1136            AF489570.1         13-955
            1137-1146           AU080537.1         788-797
            1147-1209           AC087898.19        72220-72282         c
            1210-1532           AF489572.1         903-1225
            1533-1607           AC087898.19        66312-66386         c
            1608-2004           AF489572.1         1301-1697
            2005-2010           AC087898.19        62564-62569         c
            2011-2196           AF489572.1         1704-1889
            2197-2225           AC087898.19        61381-61409         c
            2226-2293           AC087898.19        61194-61261         c
            2294-3436           AF489572.1         1987-3129
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16"
     gene            1..3436
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /note="Eef1akmt4-endothelin converting enzyme 2
                     readthrough"
                     /db_xref="GeneID:110599584"
                     /db_xref="MGI:MGI:6121518"
     exon            1..220
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..2757
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /EC_number="3.4.24.71"
                     /EC_number="2.1.1.-"
                     /note="isoform a is encoded by transcript variant 1; EEF1A
                     lysine methyltransferase 4; EEF1AKMT4-ECE2 readthrough
                     transcript protein"
                     /codon_start=1
                     /product="Eef1akmt4-Ece2 readthrough isoform a"
                     /protein_id="NP_808810.1"
                     /db_xref="GeneID:110599584"
                     /db_xref="MGI:MGI:6121518"
                     /translation="MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGD
                     FASFRALLEPELCPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSPVVVAAMQVRYA
                     HVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGEPDPWNVSSEGVHTVDQVLSE
                     MVEYKRAKLRDEESPEITVEGRATRDSLEVGFQKRTRQLFGSHTQLELVLAGLILVLA
                     ALLLGCLVALWVHRDPAHSTCVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRR
                     NPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEK
                     LGAKPLRDLIDKIGGWNITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSN
                     IIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLEL
                     EIQLANITVPQDQRRDEEKIYHKMSISELQALAPAVDWLEFLSFLLSPLELGDSEPVV
                     VYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKK
                     SCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVW
                     MDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSA
                     KVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGG
                     IGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVNGER
                     LNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGLTNHQLFFVGFAQVWCS
                     VRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW"
     exon            221..504
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            505..591
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            592..721
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            722..937
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1062
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1209
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1275
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1464
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1607
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1722
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1833
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1932
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2010
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..2114
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2225
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2293
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2484
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2580
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..3436
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3322..3327
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /note="hexamer: AATAGA"
     polyA_site      3354
                     /gene="Eef1ece2"
                     /gene_synonym="Eef1akmt4; Eef1akmt4-ece2; Gm49333"
                     /note="major polyA site"
ORIGIN      
        1 cctggctgcg gcgctgccct gagcatggcc tctccccgga ctccggtgtc ccctccggag
       61 ttaccggaga agaacttcca gtaccgccaa gtccagtact gggaccagcg ctacaaagac
      121 gcggccgact ctggacccta cgagtggttc ggagacttcg cttccttccg tgccctccta
      181 gagccggagc tgtgtcctga ggaccgtatc ctcgtgctag gttgcgggaa cagtgccttg
      241 agctatgagc tgttccttgg aggcttccct aatgtgacca gtgtggatta ctctccagta
      301 gtggtggcag ccatgcaagt tcgctatgcc catgtaccca gtctacgctg ggagaccatg
      361 gatgttcgag cactggactt ccctagtggg tcttttgatg ttgtcctcga gaagggtact
      421 ctggatgcca tgctggctgg ggagccagat ccctggaatg tatcttctga aggtgtccac
      481 actgtggacc aggtgctgag tgagatggtg gagtacaaac gtgctaagct tcgggatgaa
      541 gaatcaccag agattacagt tgaaggcagg gccacccggg actcactgga ggtgggattc
      601 cagaagagga caagacaact gtttggttca cacacacagt tggagctggt cttggcaggc
      661 ctcattctag tgttggctgc ccttcttttg ggctgcctcg tggctctgtg ggtccacaga
      721 gacccagccc atagcacctg cgtcacagaa gcctgcattc gagtagctgg aaaaatcctg
      781 gagtctctag accgtggggt gagcccctgt caggactttt accagttctc ctgtggaggc
      841 tggattcgaa gaaaccctct acccaatgga cgttctcgct ggaacacctt caacagcctc
      901 tgggaccaga accaggccat actgaagcac ctacttgaga acaccacttt caattccagc
      961 agtgaagctg agaggaagac tcggagtttc tacctgtcct gcctacagtc ggagcgcatt
     1021 gagaagctag gagccaagcc acttagagac ctcattgaca agatcggtgg ttggaacata
     1081 acggggcctt gggacgagga cagcttcatg gatgtgctca aggcagtcgc agggacctac
     1141 agagccaccc ccttcttcac cgtctacgtc agtgctgatt ctaagagttc taacagcaat
     1201 atcatccagg tggaccagtc tgggcttttt ctgccctctc gagattacta cctaaataga
     1261 actgccaatg agaaagttct cactgcctac ctggactaca tggtggagct gggagtgctg
     1321 ctgggtggac agccgacctc cactcgggag cagatgcagc aggtgctgga gctggagata
     1381 cagctggcta acatcactgt gccccaggac cagcggcgtg atgaggagaa gatctatcac
     1441 aagatgagca tctcagagct gcaggctctc gcgcccgccg tggactggct ggagttcctt
     1501 tctttcttgt tatcgccact tgagttgggt gattctgagc ctgtggtggt gtatgggact
     1561 gagtatttac agcaggtgtc ggagctcatc aaccgtactg aaccaagcat cctgaacaat
     1621 tacctaattt ggaacctggt acagaagacg acctcaagcc ttgaccagcg ctttgagact
     1681 gcacaggaga aactgctgga gaccctctac ggtaccaaga agtcctgcac tccgaggtgg
     1741 cagacctgca tctccaatac agatgatgcc cttggctttg ctctgggttc actctttgtg
     1801 aaagccacat ttgaccgaca aagcaaggaa atcgccgagg ggatgatcaa tgaaatccgc
     1861 tctgcttttg aggagaccct gggagacttg gtttggatgg atgagaagac ccggctggca
     1921 gccaaggaga aagcagatgc catctatgat atgattggtt tccctgattt catcctggag
     1981 cccaaagagc tggatgatgt ttatgatggg tatgaagtct ctgaagattc ttttttccaa
     2041 aacatgttga atctgtacaa cttctcagct aaggtgatgg ctgaccagct ccgcaaacct
     2101 cccagccgag accagtggag catgacacct cagaccgtga acgcttacta ccttccaacc
     2161 aagaatgaaa tcgtcttccc tgctggcatc ttgcaggccc ccttctatgc tcacaaccat
     2221 ccaaaggcct tgaactttgg tggcatcggc gtagtgatgg gccatgagtt gacacatgcc
     2281 tttgatgacc aagggcgtga gtatgacaaa gaagggaatc tgcgtccttg gtggcagaat
     2341 gagtcactga cggctttcca gaaccataca gcctgcatgg aagaacagta cagccagtac
     2401 caggtcaatg gagagaggct caatggactc cagaccctgg gggaaaacat cgccgataat
     2461 gggggcctta aggctgctta caatgcttac aaagcatggc tgagaaagca tggggaggag
     2521 cagccgctgc ctgctgtggg gctcaccaat caccagcttt tcttcgtggg atttgctcag
     2581 gtgtggtgct cggtccgcac accagagagc tctcacgagg ggctggtgac cgacccccac
     2641 agccctgccc gtttccgagt gctgggcact ctctccaact cccgagactt ccttcggcac
     2701 ttcggctgcc ctgtcggctc ccccatgaac ccagggcagc tatgtgaggt gtggtagacc
     2761 tgggtgggga aagaaatggg caactgtggc cagggactgg ggcttccaag ttaggagaga
     2821 gtgaagatca gctggtatgg ttgtctctcc acactgcaag tgagatcaat ctagacccca
     2881 ccaccaccac cacattgtgc ctctgcttta ggggtgcctg agcctctagc agagattcca
     2941 ccattcactg tgacatcctt tcatgtgtca ccctggaggg agtctaggca gaagtgtcag
     3001 ttcccacaga gatgagtctg tctcttttgg actccaagct tactcagttt aggggatcaa
     3061 gaggatatgc ctggggcatg gccatctctc cccagggtat actgagtgct ctatgagatg
     3121 agttcagctc ccttcagccc accctcaggc ttccccccac ctatgttcct tacgctcctc
     3181 ctagtactgc tgctcccttc aggacagacc aactgaagtc atgtggaagg ccaagggctc
     3241 tctcgggtga gaggagaaat atgtatgtac agtgggtgct caaggtgtga gtcttaggga
     3301 taactgattg tccctgggcc aaatagaaaa gcagatcgag tagagaaaag gaaagagttt
     3361 atttttgcag ggaagagggt ggggagggtg tggtcttgcc tttataggac ccgttgctaa
     3421 taaatagatc tatatc
//
