LOCUS       NM_145537               2294 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus ER degradation enhancer, mannosidase alpha-like 2
            (Edem2), mRNA.
ACCESSION   NM_145537
VERSION     NM_145537.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Slominska-Wojewodzka,M., Pawlik,A., Sokolowska,I., Antoniewicz,J.,
            Wegrzyn,G. and Sandvig,K.
  TITLE     The role of EDEM2 compared with EDEM1 in ricin transport from the
            endoplasmic reticulum to the cytosol
  JOURNAL   Biochem J 457 (3), 485-496 (2014)
   PUBMED   24200403
  REMARK    GeneRIF: Data suggest Edem1/Edem2 participate in ricin
            translocation; overexpression of Edem2, in contrast to Edem1,
            directly promotes ricin subunit A transport from endoplasmic
            reticulum (ER) to cytosol; this translocation is not dependent on
            ER translocon.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY289191.1, AK166998.1 and AL929233.9.
            
            On Jul 21, 2009 this sequence version replaced NM_145537.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029164.1, AK079296.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                BY289191.1         2-66
            66-1942             AK166998.1         2-1878
            1943-2294           AL929233.9         10549-10900         c
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 77.26 cM"
     gene            1..2294
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /note="ER degradation enhancer, mannosidase alpha-like 2"
                     /db_xref="GeneID:108687"
                     /db_xref="MGI:MGI:1915540"
     exon            1..245
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1872
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /note="ER degradation-enhancing alpha-mannosidase-like
                     protein 2"
                     /codon_start=1
                     /product="ER degradation-enhancing alpha-mannosidase-like
                     protein 2 precursor"
                     /protein_id="NP_663512.2"
                     /db_xref="CCDS:CCDS16953.1"
                     /db_xref="GeneID:108687"
                     /db_xref="MGI:MGI:1915540"
                     /translation="MPFRLLIPLGLVCVLLPLHHGAPGPDGTAPDPAHYRERVKAMFY
                     HAYDSYLENAFPYDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNTSEFQRVVEVLQ
                     DNVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARK
                     LLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVA
                     RVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKL
                     MAMFLEYNKAIRNYTHFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMR
                     TFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLEL
                     GRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFHPNNFIHNN
                     GSTFDSVMTPHGECILGAGGYIFNTEAHPIDPAALHCCRRLKEEQWEVEDLIKEFYSL
                     KQSRPKRAQRKTVRSGPWEPQSGPATLSSPANQPREKQPAQQRTPLLSCPSQPFTSKL
                     ALLGQVFLDSS"
     exon            246..356
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            357..396
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            397..502
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            503..628
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            629..840
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            841..982
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1107
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1252
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1374
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..2294
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1921..1926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1950
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /note="major polyA site"
     regulatory      1965..1970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
                     /note="hexamer: GATAAA"
     polyA_site      1988
                     /gene="Edem2"
                     /gene_synonym="9530090G24Rik"
ORIGIN      
        1 agtcaggaag tgagaggctt cttgcccaac gcgcaggcgt cgtggtgcgt atccccggaa
       61 gaaccagccg cggctcggag gatgagctgg tggcggtggc tgcagctgct gcggcggcac
      121 tgacaggaca cgagctctat gcctttccgg ctactcatcc cgctcggcct cgtgtgcgtg
      181 ctgctacccc tgcaccatgg tgcgccaggc cccgacggca ccgcgcccga ccccgcccac
      241 tacagggagc gagtcaaggc catgttctac cacgcctacg acagttacct ggaaaatgcc
      301 tttccctacg atgagctgag acctctcacc tgtgatgggc acgacacctg gggcagtttt
      361 tctctgacgc taattgatgc cctggacacc ttgctgattt tggggaatac ctctgaattc
      421 caaagagtag tggaggttct tcaggacaat gtggactttg atatcgacgt gaacgcctct
      481 gtgttcgaaa ccaacatccg agtggtagga ggactccttt ctgctcatct cttgtcaaag
      541 aaggccggtg tggaggtgga agccgggtgg ccttgttctg ggcctctcct caggatggcg
      601 gaggaagcag cccggaaact cctcccagca tttcagaccc ccacgggcat gccctatgga
      661 actgtgaacc tgcttcatgg cgtgaaccct ggagagaccc ctgttacctg tacagcaggg
      721 attgggacct tcattgtgga atttgctacc ctgagcagcc tcaccggtga ccctgtgttt
      781 gaagatgtgg ccagagtggc cctgatgcgt ctctgggaga gccggtcaga tatcgggctg
      841 gttggcaatc acatcgatgt gctcactggc aagtgggtgg cccaggatgc aggcatcggg
      901 gctggtgtgg attcctattt tgagtacctg gtaaaaggag ccatccttct tcaggataag
      961 aaactcatgg ccatgttcct agagtacaac aaagccatcc ggaattacac gcacttcgat
     1021 gactggtacc tctgggtgca gatgtacaag gggaccgtgt caatgcctgt cttccagtcc
     1081 ttggaggcct actggcctgg ccttcagagc cttattggag acattgacaa tgccatgagg
     1141 acctttctca actactacac tgtatggaag cagtttgggg ggctgccaga attctacaac
     1201 attcctcagg gatacacagt ggagaagcga gaaggctacc cactccgacc ggagctcatc
     1261 gagagcgcaa tgtacctcta ccgcgccact ggggatccca ctctcttaga acttgggaga
     1321 gacgctgtgg aatccattga gaaaatcagc aaggtggagt gtggatttgc cacgatcaag
     1381 gacctccggg accacaagct tgacaaccgc atggagtctt tcttcctggc tgaaactgtg
     1441 aaatacctct atcttctgtt ccacccaaac aacttcatcc acaacaatgg ctccaccttt
     1501 gactctgtga tgactcccca tggggagtgc atcctaggag ccgggggcta catcttcaac
     1561 acggaggctc accccattga ccctgctgcc ttgcactgct gccggaggct caaggaggag
     1621 cagtgggaag tggaggactt gataaaggag ttctactccc tcaaacagag caggccaaag
     1681 agagctcaga ggaagacagt gaggtcgggg ccctgggagc cccagtcagg gccagccacg
     1741 ctctcctcac ctgcaaacca gcccagggag aagcagcctg cccagcagcg aaccccgctt
     1801 ctcagctgcc ccagccagcc cttcacctct aagctggcat tgctaggcca ggttttccta
     1861 gactcctcat agccactgga taattttcta atttttattt ttttgagact aagctctaat
     1921 aataaatctg cttttggcta tcaggtttta catgctggat tcttgataaa atttcactta
     1981 aaaaataagt tttgtgctta tacaccaaga cagttcagag cccccagggc taaaaattgg
     2041 atctctcttc ctttcctggt atctttgagg aaggagatgt caccaccgtt tatttctttg
     2101 tcggtttttc caacatgcgc tttcaacttt tagttgtccc ctttatccat gtggccttcc
     2161 aggcaataca ggtttattaa gcacccttgg gtgacagcca taactgcaga gagtcagcag
     2221 cacacaaagg aaacgggttt cgttcttgtt ggaactagat tccggtagag aaaaataaac
     2281 aagtcagtct atgg
//
