LOCUS       NM_012029               1682 bp    mRNA    linear   ROD 24-DEC-2022
DEFINITION  Mus musculus ECSIT signalling integrator (Ecsit), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_012029 XM_978937
VERSION     NM_012029.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Carneiro FRG, Lepelley A, Seeley JJ, Hayden MS and Ghosh S.
  TITLE     An Essential Role for ECSIT in Mitochondrial Complex I Assembly and
            Mitophagy in Macrophages
  JOURNAL   Cell Rep 22 (10), 2654-2666 (2018)
   PUBMED   29514094
  REMARK    GeneRIF: these results demonstrate a key role of ECSIT in complex I
            function, mitochondrial reactive oxygen species production, and
            mitophagy-dependent mitochondrial quality control.
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Wen H, Ma H, Cai Q, Lin S, Lei X, He B, Wu S, Wang Z, Gao Y, Liu W,
            Liu W, Tao Q, Long Z, Yan M, Li D, Kelley KW, Yang Y, Huang H and
            Liu Q.
  TITLE     Recurrent ECSIT mutation encoding V140A triggers hyperinflammation
            and promotes hemophagocytic syndrome in extranodal NK/T cell
            lymphoma
  JOURNAL   Nat Med 24 (2), 154-164 (2018)
   PUBMED   29291352
  REMARK    GeneRIF: ECSIT-T419C knock-in mice showed higher peritoneal NADPH
            oxidase activity than mice with wild-type ECSIT in response to LPS.
            ECSIT-T419C-transfected ENKTL cell lines produced tumor necrosis
            factor (TNF)-alpha and interferon (IFN)-gamma, which induced
            macrophage activation and massive cytokine secretion in cell
            culture and mouse xenografts.
REFERENCE   3  (bases 1 to 1682)
  AUTHORS   Potter PK, Bowl MR, Jeyarajan P, Wisby L, Blease A, Goldsworthy ME,
            Simon MM, Greenaway S, Michel V, Barnard A, Aguilar C, Agnew T,
            Banks G, Blake A, Chessum L, Dorning J, Falcone S, Goosey L, Harris
            S, Haynes A, Heise I, Hillier R, Hough T, Hoslin A, Hutchison M,
            King R, Kumar S, Lad HV, Law G, MacLaren RE, Morse S, Nicol T,
            Parker A, Pickford K, Sethi S, Starbuck B, Stelma F, Cheeseman M,
            Cross SH, Foster RG, Jackson IJ, Peirson SN, Thakker RV, Vincent T,
            Scudamore C, Wells S, El-Amraoui A, Petit C, Acevedo-Arozena A,
            Nolan PM, Cox R, Mallon AM and Brown SD.
  TITLE     Novel gene function revealed by mouse mutagenesis screens for
            models of age-related disease
  JOURNAL   Nat Commun 7, 12444 (2016)
   PUBMED   27534441
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1682)
  AUTHORS   Kondo T, Watanabe M and Hatakeyama S.
  TITLE     TRIM59 interacts with ECSIT and negatively regulates NF-kappaB and
            IRF-3/7-mediated signal pathways
  JOURNAL   Biochem Biophys Res Commun 422 (3), 501-507 (2012)
   PUBMED   22588174
REFERENCE   5  (bases 1 to 1682)
  AUTHORS   West AP, Brodsky IE, Rahner C, Woo DK, Erdjument-Bromage H, Tempst
            P, Walsh MC, Choi Y, Shadel GS and Ghosh S.
  TITLE     TLR signalling augments macrophage bactericidal activity through
            mitochondrial ROS
  JOURNAL   Nature 472 (7344), 476-480 (2011)
   PUBMED   21525932
REFERENCE   6  (bases 1 to 1682)
  AUTHORS   Papin J and Subramaniam S.
  TITLE     Bioinformatics and cellular signaling
  JOURNAL   Curr Opin Biotechnol 15 (1), 78-81 (2004)
   PUBMED   15102471
  REMARK    Review article
REFERENCE   7  (bases 1 to 1682)
  AUTHORS   Xiao C, Shim JH, Kluppel M, Zhang SS, Dong C, Flavell RA, Fu XY,
            Wrana JL, Hogan BL and Ghosh S.
  TITLE     Ecsit is required for Bmp signaling and mesoderm formation during
            mouse embryogenesis
  JOURNAL   Genes Dev 17 (23), 2933-2949 (2003)
   PUBMED   14633973
  REMARK    GeneRIF: functions as an essential component in two important
            signal transduction pathways and establishes a novel role for Ecsit
            as a cofactor for Smad proteins in the bone morphogenetic protein
            signaling pathway
REFERENCE   8  (bases 1 to 1682)
  AUTHORS   Kopp E, Medzhitov R, Carothers J, Xiao C, Douglas I, Janeway CA and
            Ghosh S.
  TITLE     ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1
            signal transduction pathway
  JOURNAL   Genes Dev 13 (16), 2059-2071 (1999)
   PUBMED   10465784
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB589832.1 and AF182510.1.
            
            [WARNING] On May 11, 2023 this sequence was replaced by
            NM_012029.3.
            
            On Dec 10, 2011 this sequence version replaced NM_012029.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, and 3 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422592.201613.1,
                                           SRR13422603.463907.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                CB589832.1         1-45
            46-286              AF182510.1         1-241
            287-1682            AF182510.1         300-1695
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9"
     gene            1..1682
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /note="ECSIT signalling integrator"
                     /db_xref="GeneID:26940"
                     /db_xref="MGI:MGI:1349469"
     exon            1..79
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     exon            80..265
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     exon            266..383
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     CDS             288..1595
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /note="signaling intermediate in Toll
                     pathway-evolutionarily conserved; ECSIT homolog;
                     evolutionarily conserved signaling intermediate in Toll
                     pathway, mitochondrial"
                     /codon_start=1
                     /product="evolutionarily conserved signaling intermediate
                     in Toll pathway, mitochondrial precursor"
                     /protein_id="NP_036159.1"
                     /db_xref="CCDS:CCDS22920.1"
                     /db_xref="GeneID:26940"
                     /db_xref="MGI:MGI:1349469"
                     /translation="MSWVQVNLLVRSLSRGWGGLCRPALSGTPFAQVSLQALRGLHCS
                     AATHKDEPWLVPRPPEPQRKPIKVPAMHEDSFKPSGNRERDKASFLNAVRSFGAHNVR
                     KRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEVFRPRNVIQRIFVHYPRQQEC
                     GVAVLEQMERHGVMPSAETEFLLIQIFGRKSYPMLKFLRMKLWFTRFKNINPYPVPRD
                     LPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSDSTGMEDPTQPHIVGIQSPDQQAAL
                     ARHNPSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEEKVEEIPEEWELYYPQKLDL
                     EYSRSGWDDYEFDVDEVTEGPVFAMCMAGAHDQATLIKWIQGLQETNPTLAQIPVVFR
                     LARSTGELLTTSRLEGQSPPHSPPKGPEEDDETIQAEQQQGQS"
     exon            384..801
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     exon            802..1025
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1083
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1232
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1338
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1673
                     /gene="Ecsit"
                     /gene_synonym="Sitpec"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaagctccg tggctcaaca tgacagcgtc cagcggtccc acagagccgg ctcgggtctg
       61 cggctggcca cgtgagtaga caggacctta ctgtagctca gactgacatg ggacttacta
      121 attatcacca gctggtttga ttctggaggc catcttcctg cctatgtagt cctggctggt
      181 ctggaacttg ctatgcctat atagatcgtc tcacctcaaa ctcagaggtc tgccagccgg
      241 ggtctcccaa gtgctgagat tacaggtgtg gttgaatcta cagagacatg agctgggtgc
      301 aggtcaactt gctggtgcga agcctctcta ggggctgggg aggcctctgc aggcctgccc
      361 tttcaggaac tccctttgct caggtgtccc tccaggctct acgaggcctt cactgtagtg
      421 cagctacaca caaggatgag ccgtggttgg ttcctcgacc cccggagccc cagaggaagc
      481 ccatcaaggt tccagcaatg catgaagact cgttcaagcc atcaggaaac agggagcggg
      541 acaaggcaag cttcctgaac gcagtgcgca gctttggggc gcacaatgtg cgcaagcgcg
      601 gccacgtgga cttcatctac ctagcactgc gcaagatgcc agagtttggt gtggagcggg
      661 acttgtcagt atacaacctg ctgctggatg ttttccccaa ggaggtcttc cggccccgca
      721 acgttatcca gcgcatcttc gtccactacc cacggcagca ggaatgtggg gtcgcagtcc
      781 tggagcagat ggaacgacac ggggtcatgc ccagcgcaga gacagagttc ctactgattc
      841 agatattcgg gcgcaaaagt taccccatgc tcaagttcct gcggatgaag ctgtggttca
      901 cccgattcaa gaatatcaac ccctacccag tgccccgaga tcttccccag gaccctttgg
      961 acctggccaa gctaggcctg cgacacatgg agcctgacct cagtgctaag gtcactgtct
     1021 accagatgtc tttgcccagt gactcgacag gcatggaaga tcccacacag cctcacattg
     1081 taggaatcca gagtccagat cagcaagctg ccctggcccg ccacaaccca tccaggcctg
     1141 tttttgttga gggccccttc cctctctggc ttcgtaataa gtgtgtctac tatcacatcc
     1201 taagagctga cctgccacct cctgaggaag agaaagtaga ggagatccca gaagaatggg
     1261 agctgtacta cccacagaag ctggacctgg aatattcaag gagtggttgg gacgactatg
     1321 agtttgacgt ggatgaagtg acagaaggcc ctgtcttcgc catgtgcatg gctggtgccc
     1381 atgatcaggc aacattgatc aagtggatcc aaggcttgca ggagaccaac ccaaccctgg
     1441 cacagatccc agtggtattc cgcctggcca ggtccacggg ggagctcctg acaacttcaa
     1501 ggctggaggg acagtcccct ccccacagtc ctcccaaggg gcctgaggaa gatgatgaga
     1561 ccattcaggc agagcagcag caggggcaaa gttgagtcag atagagctgg catgaggaga
     1621 aaagactggg ctggtatgaa ataatatgga gaaaataaaa aaatcaaaac ctcaaaaaaa
     1681 aa
//
