LOCUS       NM_001409917            1954 bp    mRNA    linear   ROD 17-NOV-2024
DEFINITION  Mus musculus extracellular matrix protein 1 (Ecm1), transcript
            variant 4, mRNA.
ACCESSION   NM_001409917 XM_017319449
VERSION     NM_001409917.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Sun,C., Fan,W., Basha,S., Tian,T., Jin-Smith,B., Barkin,J., Xie,H.,
            Zhou,J., Yin,X.M., Ling,C., Sun,B., Petersen,B. and Pi,L.
  TITLE     Extracellular matrix protein 1 binds to connective tissue growth
            factor against liver fibrosis and ductular reaction
  JOURNAL   Hepatol Commun 8 (11), e0564 (2024)
   PUBMED   39470347
  REMARK    GeneRIF: Extracellular matrix protein 1 binds to connective tissue
            growth factor against liver fibrosis and ductular reaction.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Yu,Y., Lyu,C., Li,X., Yang,L., Wang,J., Li,H., Xin,Z., Xu,X.,
            Ren,C. and Yang,G.
  TITLE     Remodeling of tumor microenvironment by extracellular matrix
            protein 1a differentially regulates ovarian cancer metastasis
  JOURNAL   Cancer Lett 596, 217022 (2024)
   PUBMED   38849014
  REMARK    GeneRIF: Remodeling of tumor microenvironment by extracellular
            matrix protein 1a differentially regulates ovarian cancer
            metastasis.
REFERENCE   3  (bases 1 to 1954)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1954)
  AUTHORS   Xu,K., Fu,A., Li,Z., Miao,L., Lou,Z., Jiang,K., Lau,C., Su,T.,
            Tong,T., Bao,J., Lyu,A. and Kwan,H.Y.
  TITLE     Elevated extracellular matrix protein 1 in circulating
            extracellular vesicles supports breast cancer progression under
            obesity conditions
  JOURNAL   Nat Commun 15 (1), 1685 (2024)
   PUBMED   38402239
  REMARK    GeneRIF: Elevated extracellular matrix protein 1 in circulating
            extracellular vesicles supports breast cancer progression under
            obesity conditions.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1954)
  AUTHORS   Kong,L., Tian,Q., Guo,F., Mucignat,M.T., Perris,R., Sercu,S.,
            Merregaert,J., Di Cesare,P.E. and Liu,C.J.
  TITLE     Interaction between cartilage oligomeric matrix protein and
            extracellular matrix protein 1 mediates endochondral bone growth
  JOURNAL   Matrix Biol 29 (4), 276-286 (2010)
   PUBMED   20138147
REFERENCE   6  (bases 1 to 1954)
  AUTHORS   Sercu,S., Zhang,L. and Merregaert,J.
  TITLE     The extracellular matrix protein 1: its molecular interaction and
            implication in tumor progression
  JOURNAL   Cancer Invest 26 (4), 375-384 (2008)
   PUBMED   18443958
  REMARK    Review article
REFERENCE   7  (bases 1 to 1954)
  AUTHORS   Deckers,M.M., Smits,P., Karperien,M., Ni,J., Tylzanowski,P.,
            Feng,P., Parmelee,D., Zhang,J., Bouffard,E., Gentz,R., Lowik,C.W.
            and Merregaert,J.
  TITLE     Recombinant human extracellular matrix protein 1 inhibits alkaline
            phosphatase activity and mineralization of mouse embryonic
            metatarsals in vitro
  JOURNAL   Bone 28 (1), 14-20 (2001)
   PUBMED   11165938
REFERENCE   8  (bases 1 to 1954)
  AUTHORS   Smits,P., Bhalerao,J. and Merregaert,J.
  TITLE     Molecular cloning and characterization of the mouse Ecm1 gene and
            its 5' regulatory sequences
  JOURNAL   Gene 226 (2), 253-261 (1999)
   PUBMED   9931498
REFERENCE   9  (bases 1 to 1954)
  AUTHORS   Bhalerao,J., Tylzanowski,P., Filie,J.D., Kozak,C.A. and
            Merregaert,J.
  TITLE     Molecular cloning, characterization, and genetic mapping of the
            cDNA coding for a novel secretory protein of mouse. Demonstration
            of alternative splicing in skin and cartilage
  JOURNAL   J Biol Chem 270 (27), 16385-16394 (1995)
   PUBMED   7608209
REFERENCE   10 (bases 1 to 1954)
  AUTHORS   Capeless,C.G., Boughter,J.D. and Whitney,G.
  TITLE     Hydrolysis of sucrose octa-acetate: qualitative differences in
            taster and demistaster avoidance phenotypes
  JOURNAL   Chem Senses 19 (6), 595-607 (1994)
   PUBMED   7735839
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            MG954306.1.
            
            On Jul 25, 2022 this sequence version replaced XM_017319449.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13948564.1935823.1,
                                           SRR13422597.158747.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               MG954306.1         5679-5874           c
            197-259             MG954306.1         4545-4607           c
            260-364             MG954306.1         4318-4422           c
            365-499             MG954306.1         4114-4248           c
            500-580             MG954306.1         3939-4019           c
            581-625             MG954306.1         3319-3363           c
            626-945             MG954306.1         2778-3097           c
            946-1320            MG954306.1         2153-2527           c
            1321-1541           MG954306.1         1505-1725           c
            1542-1629           MG954306.1         1129-1216           c
            1630-1954           MG954306.1         453-777             c
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 40.91 cM"
     gene            1..1954
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /note="extracellular matrix protein 1"
                     /db_xref="GeneID:13601"
                     /db_xref="MGI:MGI:103060"
     exon            1..196
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1860
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /note="isoform 4 precursor is encoded by transcript
                     variant 4; secretory component p85"
                     /codon_start=1
                     /product="extracellular matrix protein 1 isoform 4
                     precursor"
                     /protein_id="NP_001396846.1"
                     /db_xref="GeneID:13601"
                     /db_xref="MGI:MGI:103060"
                     /translation="MGTVSRAALILACLALASAASEGAFKASDQREMTPERLFQHLHE
                     VGYAAPPSPPQTRRLRVDHSVTSLHDPPLFEEQREGREGPPHPTVCLTTACSVVQPPS
                     SPEDIPVYEEDWPTFLNPNVDKAGPAVPQEAIPLQKEQPPPQVHIEQKEIDPPAQPQE
                     EIVQKEVKPHTLAGQLPPEPRTWNPARHCQQGRRGVWGHRLDGFPPGRPSPDNLKQIC
                     LPERQHVIYGPWNLPQTGYSHLSRQGETLNVLETGYSRCCRCRSDTNRLDCLKLVWED
                     AMTQFCEAEFSVKTRPHLCCRLRGEERFSCFQKEAPRPDYLLRPCPVHQNGMSSGPQL
                     PFPPGLPTPDNVKNICLLRRFRAVPRNLPATDAIQRQLQALTRLETEFQRCCRQGHNH
                     TCTWKAWEGTLDGYCERELAIKTHPHSCCHYPPSPARDECFAHLAPYPNYDRDILTLD
                     LSRVTPNLMGQLCGSGRVLSKHKQIPGLIQNMTIRCCELPYPEQACCGEEEKLAFIEN
                     LCGPRRNSWKDPALCCDLSPEDKQINCFNTNYLRNVALVAGDTGNATGLGEQGPTRGT
                     DANPAPGSKEE"
     exon            197..259
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            260..364
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            365..499
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            500..580
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            581..625
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            626..945
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1320
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            1321..1541
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1629
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1954
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1931..1936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /note="hexamer: AATAAA"
     polyA_site      1954
                     /gene="Ecm1"
                     /gene_synonym="p85"
                     /note="major polyA site"
ORIGIN      
        1 aagttcacaa cggcagtagc aacagccagg ggagcaggct tgcagcaagt ggcccaaccc
       61 cttcaagtct agatctgcct gtgacaacca gcttctgggt gaccagtgac cagttcttgc
      121 cccaggatgg ggaccgtatc cagagcagcc ttgatcttgg cctgcttggc tcttgcttct
      181 gctgcctctg agggagcctt caaggcttca gaccagcgag agatgacgcc agagcgcctc
      241 ttccagcacc tccatgaagt aggttatgca gcaccccctt ccccaccaca aacccggaga
      301 ctccgagttg accactctgt aacttctctg catgaccctc ccctctttga ggaacaaaga
      361 gaaggaaggg agggaccccc ccaccccact gtctgcctca caactgcctg ttctgtagtg
      421 cagccccctt cctctccaga agacatccct gtgtacgagg aagactggcc cactttccta
      481 aaccctaatg tagataaagc tggtcctgct gtccctcaag aagccatccc cctgcagaaa
      541 gagcagcccc ctccccaagt ccatattgaa cagaaggaaa tagacccgcc tgcccagcct
      601 caggaggaga ttgtccagaa agaggtgaag ccacacacct tggcgggcca gctccctcca
      661 gagccccgga cttggaatcc agcccgtcac tgccagcagg gacggagagg tgtctggggc
      721 caccggctgg atggcttccc tcctggacgg ccttctccag acaatctgaa gcagatctgc
      781 cttcctgagc gtcagcatgt gatctacggc ccctggaacc tgccgcagac tggctactct
      841 caccttagtc gccagggaga gaccctcaat gtgctggaga ccggatactc ccgctgctgt
      901 cgctgccgca gcgacacaaa ccgcctagac tgtttgaagc ttgtgtggga ggatgcaatg
      961 acccaatttt gtgaggccga attctctgtc aagacccgcc cccacctgtg ctgcagactg
     1021 cgtggggagg agcgattctc ttgcttccag aaggaagctc ctcgcccaga ctacctgctc
     1081 cgaccctgcc ccgtccacca gaatggcatg tcctcagggc cccagttgcc tttccccccg
     1141 gggttgccca caccggacaa tgtcaaaaac atctgtctcc tgagacgctt ccgcgccgtg
     1201 ccacgcaacc tcccagctac tgacgccatc cagaggcagc tgcaggctct gactcggctg
     1261 gagacggagt tccagcgctg ctgccgccag ggccacaacc acacttgcac atggaaggcc
     1321 tgggagggta ccctggatgg atactgcgag cgggagctgg ctataaagac ccacccccac
     1381 tcgtgctgcc actaccctcc tagtcctgcc cgtgatgagt gcttcgccca cctagctccc
     1441 tatcccaact atgaccggga tatcttgacc cttgacctca gccgagtcac ccccaacctc
     1501 atgggccagc tctgtggaag tggaagggtc cttagcaagc ataaacagat tccggggctg
     1561 atccagaata tgaccatccg ctgctgcgag cttccatatc cagaacaggc ctgctgcggc
     1621 gaagaggaga aactggcctt catcgagaac ctctgtggtc cccggaggaa ttcgtggaaa
     1681 gaccctgccc tctgctgtga cctgtctcct gaagataagc aaatcaactg cttcaatacc
     1741 aactacctga ggaacgtggc tttagtggct ggagacactg ggaatgccac tggcttgggg
     1801 gagcagggcc caactcgggg aacagatgcc aaccccgccc ctgggtccaa ggaagaatga
     1861 gtcaccctga gcctcagagg attagatggg ggaactccgc cctactccac cctcctcgaa
     1921 cactcattac aataaatgcc tcttggattt ggca
//
