LOCUS       NM_001418546            1420 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus enoyl-Coenzyme A delta isomerase 3 (Eci3), transcript
            variant 2, mRNA.
ACCESSION   NM_001418546 XM_006516765
VERSION     NM_001418546.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1420)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 1420)
  AUTHORS   van Weeghel,M., Ofman,R., Argmann,C.A., Ruiter,J.P., Claessen,N.,
            Oussoren,S.V., Wanders,R.J., Aten,J. and Houten,S.M.
  TITLE     Identification and characterization of Eci3, a murine
            kidney-specific Delta3,Delta2-enoyl-CoA isomerase
  JOURNAL   FASEB J 28 (3), 1365-1374 (2014)
   PUBMED   24344334
  REMARK    GeneRIF: Eci3 specifically evolved in rodents after gene
            duplication of Eci2 and is localized to the peroxisomes
            GeneRIF: Mouse Eci3 is almost exclusively expressed in kidney.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124537.5.
            
            On Mar 21, 2023 this sequence version replaced XM_006516765.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.8823.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                AC124537.5         71692-71748
            58-128              AC124537.5         74708-74778
            129-227             AC124537.5         75003-75101
            228-416             AC124537.5         75440-75628
            417-486             AC124537.5         78572-78641
            487-586             AC124537.5         81321-81420
            587-707             AC124537.5         82401-82521
            708-797             AC124537.5         86668-86757
            798-941             AC124537.5         87287-87430
            942-1420            AC124537.5         88412-88890
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 14.23 cM"
     gene            1..1420
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /note="enoyl-Coenzyme A delta isomerase 3"
                     /db_xref="GeneID:69123"
                     /db_xref="MGI:MGI:1916373"
     exon            1..57
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            58..128
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            129..227
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1103
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /EC_number="5.3.3.8"
                     /note="isoform 1 is encoded by transcript variant 2;
                     kidney specific expressed protein 2; dodecenoyl-CoA
                     isomerase; D3,D2-enoyl-CoA isomerase;
                     delta(3),delta(2)-enoyl-CoA isomerase; enoyl-CoA delta
                     isomerase 3, peroxisomal"
                     /codon_start=1
                     /product="enoyl-CoA delta isomerase 3, peroxisomal isoform
                     1"
                     /protein_id="NP_001405475.1"
                     /db_xref="GeneID:69123"
                     /db_xref="MGI:MGI:1916373"
                     /translation="MPKPGVFNFVNKATWDARNALGSLPKETARKNYVDLVSSLSSSS
                     EAPSQGKRGADEKARESKDILVTSEDGITKITFNRPTKKNAISFQMYLDIMHALKNAS
                     TDNSVITVFTGTGDYYSSGNDLKNLINDAGEIQDVVATSTKILREFVNCFIDFPKPLV
                     AVVNGPAVGIAVTILALFDAVFASDRATFHTPFSQLSQIPEACSTYMFPKIMGPTKAA
                     EMLLFGKKLTAREAWAQGLVTEVFPESTFETEVWTRLKTYSKLSPNVMRISKELIRKH
                     EKQKLYTVNAEECAAALERMPREEYAKALRNFLFRKAKAKL"
     exon            228..416
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            417..486
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            487..586
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            587..707
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            708..797
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            798..941
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1420
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1225..1230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /note="hexamer: AATAAA"
     polyA_site      1247
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /note="major polyA site"
     regulatory      1386..1391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /note="hexamer: AATAAA"
     regulatory      1396..1401
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
                     /note="hexamer: ATTAAA"
     polyA_site      1406
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
     polyA_site      1420
                     /gene="Eci3"
                     /gene_synonym="1810022C23Rik; Kseg2"
ORIGIN      
        1 aaggctggct ttaacttcct tccttgagca gaagagtacg cggtgctggt gtcctagacg
       61 atcttcctga ggaccaggaa ctataagacc ccacccctac ctgcaacagc tacagccctg
      121 ttgagcaggc cacagaagga ccctgtaata tgcctaagcc tggtgtgttt aactttgtca
      181 acaaagccac gtgggatgca agaaatgccc tgggcagcct acccaaggaa actgccagga
      241 agaactatgt ggatctcgta tccagtttga gttcctcatc tgaagccccg agccagggaa
      301 agcgtggagc tgatgagaaa gcccgggagt ccaaagacat cctggtaact tctgaagatg
      361 gcatcacaaa gatcacgttc aatcggccca ccaaaaagaa tgccatatcc ttccagatgt
      421 atctggatat tatgcatgca cttaaaaatg ccagcacgga taactcagtc ataactgttt
      481 tcacaggaac tggtgactac tacagcagtg ggaatgacct aaaaaacttg attaatgacg
      541 ctggtgaaat acaggacgta gtagccacgt ctactaagat actgagggaa tttgtaaact
      601 gttttataga ctttccgaag cctctggttg cagtagtaaa tggcccagct gtgggaatcg
      661 ctgtcaccat cctggcacta tttgatgctg tctttgcttc ggacagggca acgtttcata
      721 ctccattcag ccaactcagc cagatcccgg aagcatgctc cacatacatg tttccaaaga
      781 taatgggccc aacaaaggca gctgagatgc ttctctttgg gaagaagctc acagcaagag
      841 aggcctgggc tcaaggcctt gtcactgaag ttttccctga gagcactttt gagacagaag
      901 tctggaccag gctgaaaaca tattcgaagc tctcgccaaa cgtgatgaga atttccaaag
      961 agttaatcag aaaacatgag aaacaaaagc tatacaccgt gaatgccgaa gagtgcgctg
     1021 cagccctgga aaggatgccg agagaggagt atgcgaaggc actcaggaac ttcctgttca
     1081 gaaaggcaaa ggctaagcta tgaagaccac cagagcgggc atgcactgag agtcccagtc
     1141 tcaggagctt caatgacaca gactcctctg tgcccttgtt attcctagca tagcagttac
     1201 gaggagagtg cctcagctat gaccaataaa aactcccata aaacaaagcc ttatgagttt
     1261 atgcaggata gctagaattg acctgaccca gagggtgata gacctctaca atgaaacttt
     1321 acatctctga acaaagagat ataccgaaaa actctagaaa atggaaagac aacctatagt
     1381 tcatgaataa aattaattaa aaatgatact aaaaaattaa
//
