LOCUS       NM_139293               3129 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Mus musculus endothelin converting enzyme 2 (Ece2), transcript
            variant 4, mRNA.
ACCESSION   NM_139293
VERSION     NM_139293.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Wang,N., Langfelder,P., Stricos,M., Ramanathan,L., Richman,J.B.,
            Vaca,R., Plascencia,M., Gu,X., Zhang,S., Tamai,T.K., Zhang,L.,
            Gao,F., Ouk,K., Lu,X., Ivanov,L.V., Vogt,T.F., Lu,Q.R.,
            Morton,A.J., Colwell,C.S., Aaronson,J.S., Rosinski,J., Horvath,S.
            and Yang,X.W.
  TITLE     Mapping brain gene coexpression in daytime transcriptomes unveils
            diurnal molecular networks and deciphers perturbation gene
            signatures
  JOURNAL   Neuron 110 (20), 3318-3338 (2022)
   PUBMED   36265442
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Pacheco-Quinto,J., Eckman,C.B. and Eckman,E.A.
  TITLE     Major amyloid-beta-degrading enzymes, endothelin-converting
            enzyme-2 and neprilysin, are expressed by distinct populations of
            GABAergic interneurons in hippocampus and neocortex
  JOURNAL   Neurobiol Aging 48, 83-92 (2016)
   PUBMED   27644077
  REMARK    GeneRIF: It is a amyloid beta degrading enzyme and is expressed by
            distinct populations of GABAergic interneurons in hippocampus and
            neocortex.
REFERENCE   3  (bases 1 to 3129)
  AUTHORS   Gupta,A., Fujita,W., Gomes,I., Bobeck,E. and Devi,L.A.
  TITLE     Endothelin-converting enzyme 2 differentially regulates opioid
            receptor activity
  JOURNAL   Br J Pharmacol 172 (2), 704-719 (2015)
   PUBMED   24990314
  REMARK    GeneRIF: These results suggest that ECE2, by selectively processing
            endogenous opioid peptides in the endocytic compartment, plays a
            role in modulating opioid receptor activity
REFERENCE   4  (bases 1 to 3129)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   5  (bases 1 to 3129)
  AUTHORS   Gupta,A., Gomes,I., Wardman,J. and Devi,L.A.
  TITLE     Opioid receptor function is regulated by post-endocytic peptide
            processing
  JOURNAL   J Biol Chem 289 (28), 19613-19626 (2014)
   PUBMED   24847082
  REMARK    GeneRIF: a role for differential modulation of opioid receptor
            signaling by post-endocytic processing of peptide agonists by ECE2.
REFERENCE   6  (bases 1 to 3129)
  AUTHORS   Eckman,E.A., Watson,M., Marlow,L., Sambamurti,K. and Eckman,C.B.
  TITLE     Alzheimer's disease beta-amyloid peptide is increased in mice
            deficient in endothelin-converting enzyme
  JOURNAL   J Biol Chem 278 (4), 2081-2084 (2003)
   PUBMED   12464614
  REMARK    GeneRIF: ECE2 has a role in limiting Abeta accumulation in the
            mouse brain
REFERENCE   7  (bases 1 to 3129)
  AUTHORS   Ikeda,S., Emoto,N., Alimsardjono,H., Yokoyama,M. and Matsuo,M.
  TITLE     Molecular isolation and characterization of novel four subisoforms
            of ECE-2
  JOURNAL   Biochem Biophys Res Commun 293 (1), 421-426 (2002)
   PUBMED   12054617
  REMARK    GeneRIF: novel four subisoforms of ECE-2 that differ in their
            N-terminal cytoplasmic tails
REFERENCE   8  (bases 1 to 3129)
  AUTHORS   Zhang,J., Leontovich,A. and Sarras,M.P. Jr.
  TITLE     Molecular and functional evidence for early divergence of an
            endothelin-like system during metazoan evolution: analysis of the
            Cnidarian, hydra
  JOURNAL   Development 128 (9), 1607-1615 (2001)
   PUBMED   11290299
REFERENCE   9  (bases 1 to 3129)
  AUTHORS   Yanagisawa,H., Hammer,R.E., Richardson,J.A., Emoto,N.,
            Williams,S.C., Takeda,Si., Clouthier,D.E. and Yanagisawa,M.
  TITLE     Disruption of ECE-1 and ECE-2 reveals a role for
            endothelin-converting enzyme-2 in murine cardiac development
  JOURNAL   J Clin Invest 105 (10), 1373-1382 (2000)
   PUBMED   10811845
REFERENCE   10 (bases 1 to 3129)
  AUTHORS   Emoto,N. and Yanagisawa,M.
  TITLE     Endothelin-converting enzyme-2 is a membrane-bound,
            phosphoramidon-sensitive metalloprotease with acidic pH optimum
  JOURNAL   J Biol Chem 270 (25), 15262-15268 (1995)
   PUBMED   7797512
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF489572.1, AU080537.1 and AC087898.19.
            
            On Jul 26, 2007 this sequence version replaced NM_139293.1.
            
            Transcript Variant: This variant (4) differs in the 5' UTR and CDS
            compared to variant 1. The resulting isoform (d) has a shorter and
            distinct N-terminus compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF489572.1, AF396699.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-829               AF489572.1         1-829
            830-839             AU080537.1         788-797
            840-902             AC087898.19        72220-72282         c
            903-1225            AF489572.1         903-1225
            1226-1300           AC087898.19        66312-66386         c
            1301-1697           AF489572.1         1301-1697
            1698-1703           AC087898.19        62564-62569         c
            1704-1889           AF489572.1         1704-1889
            1890-1918           AC087898.19        61381-61409         c
            1919-1986           AC087898.19        61194-61261         c
            1987-3129           AF489572.1         1987-3129
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 12.48 cM"
     gene            1..3129
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /note="endothelin converting enzyme 2"
                     /db_xref="GeneID:107522"
                     /db_xref="MGI:MGI:1101356"
     exon            1..197
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..2450
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /EC_number="3.4.24.71"
                     /note="isoform d is encoded by transcript variant 4;
                     EEF1AKMT4-ECE2 readthrough transcript protein"
                     /codon_start=1
                     /product="EEF1AKMT4-ECE2 readthrough transcript protein
                     isoform d"
                     /protein_id="NP_647454.2"
                     /db_xref="CCDS:CCDS28052.1"
                     /db_xref="GeneID:107522"
                     /db_xref="MGI:MGI:1101356"
                     /translation="MNVALHELGGGGSMVEYKRAKLRDEESPEITVEGRATRDSLEVG
                     FQKRTRQLFGSHTQLELVLAGLILVLAALLLGCLVALWVHRDPAHSTCVTEACIRVAG
                     KILESLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENT
                     TFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNITGPWDEDSFMDVL
                     KAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYL
                     DYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQA
                     LAPAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLV
                     QKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFD
                     RQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKE
                     LDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTK
                     NEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQ
                     NESLTAFQNHTACMEEQYSQYQVNGERLNGLQTLGENIADNGGLKAAYNAYKAWLRKH
                     GEEQPLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSR
                     DFLRHFGCPVGSPMNPGQLCEVW"
     exon            198..284
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            285..414
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            415..630
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            631..755
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            756..902
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            903..968
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1157
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1300
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1415
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1526
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1625
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1703
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1807
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1918
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..1986
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2177
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2273
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..3129
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3015..3020
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /note="hexamer: AATAGA"
     polyA_site      3047
                     /gene="Ece2"
                     /gene_synonym="1810009K13Rik; 6330509A19Rik;
                     9630025D12Rik; Ece-2; Eef1akmt4-Ece2"
                     /note="major polyA site"
ORIGIN      
        1 acctgagaaa gccgggccgc cgcggccaag ccaggggctg cagcagtgac tgtgatggag
       61 ggcagcggcg gggttcagcg ggtggcctgg gccgtagttc ctgggcgggt caacctcgcc
      121 cagccctaag gcgcggcccg gccttgctcc actccaccat gaacgtcgcg ctgcacgagt
      181 tgggtggcgg aggcagtatg gtggagtaca aacgtgctaa gcttcgggat gaagaatcac
      241 cagagattac agttgaaggc agggccaccc gggactcact ggaggtggga ttccagaaga
      301 ggacaagaca actgtttggt tcacacacac agttggagct ggtcttggca ggcctcattc
      361 tagtgttggc tgcccttctt ttgggctgcc tcgtggctct gtgggtccac agagacccag
      421 cccatagcac ctgcgtcaca gaagcctgca ttcgagtagc tggaaaaatc ctggagtctc
      481 tagaccgtgg ggtgagcccc tgtcaggact tttaccagtt ctcctgtgga ggctggattc
      541 gaagaaaccc tctacccaat ggacgttctc gctggaacac cttcaacagc ctctgggacc
      601 agaaccaggc catactgaag cacctacttg agaacaccac tttcaattcc agcagtgaag
      661 ctgagaggaa gactcggagt ttctacctgt cctgcctaca gtcggagcgc attgagaagc
      721 taggagccaa gccacttaga gacctcattg acaagatcgg tggttggaac ataacggggc
      781 cttgggacga ggacagcttc atggatgtgc tcaaggcagt cgcagggacc tacagagcca
      841 cccccttctt caccgtctac gtcagtgctg attctaagag ttctaacagc aatatcatcc
      901 aggtggacca gtctgggctt tttctgccct ctcgagatta ctacctaaat agaactgcca
      961 atgagaaagt tctcactgcc tacctggact acatggtgga gctgggagtg ctgctgggtg
     1021 gacagccgac ctccactcgg gagcagatgc agcaggtgct ggagctggag atacagctgg
     1081 ctaacatcac tgtgccccag gaccagcggc gtgatgagga gaagatctat cacaagatga
     1141 gcatctcaga gctgcaggct ctcgcgcccg ccgtggactg gctggagttc ctttctttct
     1201 tgttatcgcc acttgagttg ggtgattctg agcctgtggt ggtgtatggg actgagtatt
     1261 tacagcaggt gtcggagctc atcaaccgta ctgaaccaag catcctgaac aattacctaa
     1321 tttggaacct ggtacagaag acgacctcaa gccttgacca gcgctttgag actgcacagg
     1381 agaaactgct ggagaccctc tacggtacca agaagtcctg cactccgagg tggcagacct
     1441 gcatctccaa tacagatgat gcccttggct ttgctctggg ttcactcttt gtgaaagcca
     1501 catttgaccg acaaagcaag gaaatcgccg aggggatgat caatgaaatc cgctctgctt
     1561 ttgaggagac cctgggagac ttggtttgga tggatgagaa gacccggctg gcagccaagg
     1621 agaaagcaga tgccatctat gatatgattg gtttccctga tttcatcctg gagcccaaag
     1681 agctggatga tgtttatgat gggtatgaag tctctgaaga ttcttttttc caaaacatgt
     1741 tgaatctgta caacttctca gctaaggtga tggctgacca gctccgcaaa cctcccagcc
     1801 gagaccagtg gagcatgaca cctcagaccg tgaacgctta ctaccttcca accaagaatg
     1861 aaatcgtctt ccctgctggc atcttgcagg cccccttcta tgctcacaac catccaaagg
     1921 ccttgaactt tggtggcatc ggcgtagtga tgggccatga gttgacacat gcctttgatg
     1981 accaagggcg tgagtatgac aaagaaggga atctgcgtcc ttggtggcag aatgagtcac
     2041 tgacggcttt ccagaaccat acagcctgca tggaagaaca gtacagccag taccaggtca
     2101 atggagagag gctcaatgga ctccagaccc tgggggaaaa catcgccgat aatgggggcc
     2161 ttaaggctgc ttacaatgct tacaaagcat ggctgagaaa gcatggggag gagcagccgc
     2221 tgcctgctgt ggggctcacc aatcaccagc ttttcttcgt gggatttgct caggtgtggt
     2281 gctcggtccg cacaccagag agctctcacg aggggctggt gaccgacccc cacagccctg
     2341 cccgtttccg agtgctgggc actctctcca actcccgaga cttccttcgg cacttcggct
     2401 gccctgtcgg ctcccccatg aacccagggc agctatgtga ggtgtggtag acctgggtgg
     2461 ggaaagaaat gggcaactgt ggccagggac tggggcttcc aagttaggag agagtgaaga
     2521 tcagctggta tggttgtctc tccacactgc aagtgagatc aatctagacc ccaccaccac
     2581 caccacattg tgcctctgct ttaggggtgc ctgagcctct agcagagatt ccaccattca
     2641 ctgtgacatc ctttcatgtg tcaccctgga gggagtctag gcagaagtgt cagttcccac
     2701 agagatgagt ctgtctcttt tggactccaa gcttactcag tttaggggat caagaggata
     2761 tgcctggggc atggccatct ctccccaggg tatactgagt gctctatgag atgagttcag
     2821 ctcccttcag cccaccctca ggcttccccc cacctatgtt ccttacgctc ctcctagtac
     2881 tgctgctccc ttcaggacag accaactgaa gtcatgtgga aggccaaggg ctctctcggg
     2941 tgagaggaga aatatgtatg tacagtgggt gctcaaggtg tgagtcttag ggataactga
     3001 ttgtccctgg gccaaataga aaagcagatc gagtagagaa aaggaaagag tttatttttg
     3061 cagggaagag ggtggggagg gtgtggtctt gcctttatag gacccgttgc taataaatag
     3121 atctatatc
//
